DE68929531D1 - Expressionssystem für die Sekretion von bioaktivem, menschlichem granulozyt-makrophagem Koloniestimulierungsfaktor (GM-CSF) und anderen heterologen Proteinen aus Streptomyces - Google Patents

Expressionssystem für die Sekretion von bioaktivem, menschlichem granulozyt-makrophagem Koloniestimulierungsfaktor (GM-CSF) und anderen heterologen Proteinen aus Streptomyces

Info

Publication number
DE68929531D1
DE68929531D1 DE68929531T DE68929531T DE68929531D1 DE 68929531 D1 DE68929531 D1 DE 68929531D1 DE 68929531 T DE68929531 T DE 68929531T DE 68929531 T DE68929531 T DE 68929531T DE 68929531 D1 DE68929531 D1 DE 68929531D1
Authority
DE
Germany
Prior art keywords
csf
expression system
bioactive
streptomyces
secretion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE68929531T
Other languages
English (en)
Inventor
Robert T Garvin
Lawrence T Malek
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cangene Corp
Original Assignee
Cangene Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cangene Corp filed Critical Cangene Corp
Application granted granted Critical
Publication of DE68929531D1 publication Critical patent/DE68929531D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Toxicology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
DE68929531T 1988-07-25 1989-07-24 Expressionssystem für die Sekretion von bioaktivem, menschlichem granulozyt-makrophagem Koloniestimulierungsfaktor (GM-CSF) und anderen heterologen Proteinen aus Streptomyces Expired - Lifetime DE68929531D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CA000572956A CA1295567C (en) 1988-07-25 1988-07-25 Expression system for the secretion of bioactive human granulocyte, macrophage-colony stimulating factor (gm-csf) and other heterologous proteins from streptomyces

Publications (1)

Publication Number Publication Date
DE68929531D1 true DE68929531D1 (de) 2005-04-28

Family

ID=4138439

Family Applications (1)

Application Number Title Priority Date Filing Date
DE68929531T Expired - Lifetime DE68929531D1 (de) 1988-07-25 1989-07-24 Expressionssystem für die Sekretion von bioaktivem, menschlichem granulozyt-makrophagem Koloniestimulierungsfaktor (GM-CSF) und anderen heterologen Proteinen aus Streptomyces

Country Status (5)

Country Link
EP (1) EP0352707B1 (de)
JP (2) JP2711148B2 (de)
AT (1) ATE291629T1 (de)
CA (1) CA1295567C (de)
DE (1) DE68929531D1 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR930701464A (ko) * 1990-07-11 1993-06-11 스튜어트 알.슈터 이질성 단백질의 생성용 스트렙토마이세스 벡터
FR2666588B1 (fr) * 1990-09-10 1994-02-11 Pasteur Institut Sequence nucleotidique regulatrice de l'initiation de transcription.
JP2818146B2 (ja) * 1996-06-20 1998-10-30 株式会社精工技研 光ファイバ端面研磨装置
JP2787293B2 (ja) * 1996-06-20 1998-08-13 株式会社精工技研 光ファイバ端面研磨装置

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4775622A (en) * 1982-03-08 1988-10-04 Genentech, Inc. Expression, processing and secretion of heterologous protein by yeast
GB8315579D0 (en) * 1983-06-07 1983-07-13 Biogen Nv Streptomyces expression systems
ATE67244T1 (de) * 1984-07-06 1991-09-15 Sandoz Ag Herstellung und reinigung von lymphokinen.
US4745056A (en) * 1984-10-23 1988-05-17 Biotechnica International, Inc. Streptomyces secretion vector
CA1295563C (en) * 1985-11-01 1992-02-11 Robert T. Garvin Production of active proteins containing cystine residues
CA1295566C (en) * 1987-07-21 1992-02-11 Robert T. Garvin Characterization and structure of genes for protease a and protease b from streptomyces griseus
AU1299988A (en) * 1987-03-09 1988-10-10 Cetus Corporation Expression of heterologous genes in streptomyces species

Also Published As

Publication number Publication date
EP0352707B1 (de) 2005-03-23
JPH0286782A (ja) 1990-03-27
JPH0767663A (ja) 1995-03-14
JP2711148B2 (ja) 1998-02-10
ATE291629T1 (de) 2005-04-15
CA1295567C (en) 1992-02-11
JP2688174B2 (ja) 1997-12-08
EP0352707A2 (de) 1990-01-31
EP0352707A3 (de) 1991-09-11

Similar Documents

Publication Publication Date Title
ATE103932T1 (de) Fusionsprotein bestehend aus gm-csf und il-3.
EP1323737A3 (de) Blutbildendes Fusionsprotein mit Il-3 Varianten
ATE312844T1 (de) Biologisch-aktive bakterizide/permeabilitätserhöhende proteinbruchstücke
AU8007087A (en) New forms of colony stimulating factor-1
HU9200548D0 (en) Improved activity process of recombined proteins
SE8101005L (sv) Mikrobiologiskt framstelld polypeptid med samma aminosyrasekvens som hos humant interferon, dna och plasmider, som koderar for denna sekvens, mikroorganismer, som innehaller dessa genetiska informationer och forfaran...
IT8421436A1 (it) Un plasmide ricombinante, un microorganismo trasformante, un segmento di polideossiribonucleotide, un procedimento per produrre una proteina biologicamente attiva e la proteina così prodotta
ATE101203T1 (de) Arab-promoter und verfahren zur herstellung von polypeptiden einschliesslich cecropinen mittels mikrobiologischer verfahren.
AU637139B2 (en) Gm-csf protein, its derivatives, the preparation of proteins of this type, and their use
AU596997C (en) Pharmaceutical composition for the treatment of leukopenia
DE68929531D1 (de) Expressionssystem für die Sekretion von bioaktivem, menschlichem granulozyt-makrophagem Koloniestimulierungsfaktor (GM-CSF) und anderen heterologen Proteinen aus Streptomyces
HUT50195A (en) New peptites hindering the activity of immune system and medical compositions containing them and process for production of these peptides and compositions
SE8101004L (sv) Mikrobiologiskt framstelld polypeptid med samma aminosyrasekvens som hos humant interferon, dna och plasmider, som koderar for denna sekvens, mikroorganismer, som innehaller dessa genetiska informationer och forfarande
ATE254179T1 (de) Neue type-i-interferon varianten, ihre verfahren zur herstellung, und ihre verwendungen
IT8719551A0 (it) Plasmidi ricombinanti utili perl'espressione in bacillus di proteine eterologhe.
EP0723016A3 (de) Leukocyten-aktivierender Faktor
ES2162801T3 (es) Factor que regula la expresion genica.

Legal Events

Date Code Title Description
8332 No legal effect for de