DE68925138T2 - Verfahren zur Herstellung von Proteinen durch Verwendung von CKS-Fusionsproteinen - Google Patents
Verfahren zur Herstellung von Proteinen durch Verwendung von CKS-FusionsproteinenInfo
- Publication number
- DE68925138T2 DE68925138T2 DE68925138T DE68925138T DE68925138T2 DE 68925138 T2 DE68925138 T2 DE 68925138T2 DE 68925138 T DE68925138 T DE 68925138T DE 68925138 T DE68925138 T DE 68925138T DE 68925138 T2 DE68925138 T2 DE 68925138T2
- Authority
- DE
- Germany
- Prior art keywords
- proteins
- production
- cks fusion
- fusion proteins
- cks
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
- G01N33/56988—HIV or HTLV
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
- G01N33/5767—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis non-A, non-B hepatitis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/033—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the internal surface of the plasma membrane, e.g. containing a myristoylation motif
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/33—Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16611—Simplexvirus, e.g. human herpesvirus 1, 2
- C12N2710/16622—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
- C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US16706788A | 1988-03-11 | 1988-03-11 | |
US07/276,263 US5124255A (en) | 1988-03-11 | 1988-11-23 | CKS method of protein synthesis |
Publications (2)
Publication Number | Publication Date |
---|---|
DE68925138D1 DE68925138D1 (de) | 1996-02-01 |
DE68925138T2 true DE68925138T2 (de) | 1996-06-13 |
Family
ID=22605797
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE68925138T Expired - Fee Related DE68925138T2 (de) | 1988-03-11 | 1989-02-20 | Verfahren zur Herstellung von Proteinen durch Verwendung von CKS-Fusionsproteinen |
Country Status (10)
Country | Link |
---|---|
US (1) | US5124255A (de) |
EP (1) | EP0331961B1 (de) |
JP (1) | JPH0813273B2 (de) |
KR (1) | KR890014738A (de) |
AT (1) | ATE131876T1 (de) |
AU (1) | AU625554B2 (de) |
CA (1) | CA1335358C (de) |
DE (1) | DE68925138T2 (de) |
ES (1) | ES2083957T3 (de) |
GR (1) | GR3018698T3 (de) |
Families Citing this family (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU650038B2 (en) * | 1988-03-11 | 1994-06-09 | Abbott Laboratories | CKS method of protein synthesis |
US5312737A (en) * | 1988-03-11 | 1994-05-17 | Abbott Laboratories | CKS method of HCV protein synthesis |
CA2003383A1 (en) * | 1988-11-23 | 1990-05-23 | Sushil G. Devare | Synthetic dna derived recombinant hiv antigens |
WO1990013652A1 (en) * | 1989-05-12 | 1990-11-15 | Triton Diagnostics, Inc. | Herpes simplex virus type 2-glycoprotein g proteins and polypeptides |
DE4002636A1 (de) * | 1990-01-30 | 1991-08-01 | Boehringer Mannheim Gmbh | Expression von hiv1- und 2-polypeptiden und deren verwendung |
CA2049679C (en) * | 1990-08-24 | 2005-06-21 | Sushil G. Devare | Hepatitis c assay utilizing recombinant antigens |
US6172189B1 (en) * | 1990-08-24 | 2001-01-09 | Abbott Laboratories | Hepatitis C assay utilizing recombinant antigens |
JP3242402B2 (ja) | 1990-11-07 | 2001-12-25 | アボット・ラボラトリーズ | C型肝炎ウイルスに対するモノクローナル抗体およびその使用法 |
JPH06510289A (ja) * | 1991-08-21 | 1994-11-17 | アボツト・ラボラトリーズ | Ns5領域由来の組換え抗原を利用したc型肝炎アッセイ |
AU2513592A (en) * | 1991-08-21 | 1993-03-16 | Abbott Laboratories | Hepatitis c assay utilizing recombinant antigens to ns1 |
US5965702A (en) * | 1991-10-21 | 1999-10-12 | Abbott Laboratories | Borrelia burgdorferi antigens and uses thereof |
AU4400593A (en) * | 1992-06-05 | 1994-01-04 | Abbott Laboratories | Methods and reagents for the determination of immunosuppressive agents |
US5607914A (en) * | 1993-01-13 | 1997-03-04 | Pioneer Hi-Bred International, Inc. | Synthetic antimicrobial peptides |
CA2125467C (en) * | 1993-07-06 | 2001-02-06 | Heinz Dobeli | Process for producing hydrophobic polypeptides, proteins or peptides |
DE4405810A1 (de) | 1994-02-23 | 1995-08-24 | Behringwerke Ag | Von einem Retrovirus aus der HIV-Gruppe abgeleitete Peptide und deren Verwendung |
US6511812B1 (en) * | 1994-05-09 | 2003-01-28 | Abbott Laboratories | Method and test kit for use in improving immunoassay specificity |
EP0760099A4 (de) * | 1994-05-09 | 1998-12-09 | Abbott Lab | Verfahren und reagenzien zur verbesserung der immunotest-spezifität |
IT1266740B1 (it) * | 1994-07-01 | 1997-01-14 | Maria Paola Landini | Materiale proteico ricombinante legante anticorpi contro il citomegalovirus umano, reagenti diagnostici derivati da tale |
EP0826060B1 (de) * | 1995-05-15 | 2008-08-27 | Abbott Laboratories | Verankerte expression heterologer proteine |
US7901910B2 (en) | 1996-05-14 | 2011-03-08 | Gregory Thomas Maine | Embedded expression of insoluble heterologous proteins |
IT1290804B1 (it) | 1996-08-16 | 1998-12-11 | Abbott Lab | Saggio western blot perfezionato per l'individuazione di anticorpi specifici contro il virus hcmv |
US20050202499A1 (en) | 1996-10-31 | 2005-09-15 | Billing-Medel Patricia A. | Reagents and methods useful for detecting diseases of the breast |
US5922533A (en) * | 1997-08-15 | 1999-07-13 | Abbott Laboratories | Rapid assay for simultaneous detection and differentiation of antibodies to HIV groups |
US6846905B2 (en) * | 1997-08-15 | 2005-01-25 | Abbott Laboratories | Antigen constructs useful in the detection and differentiation of antibodies to HIV |
CA2293677C (en) * | 1998-04-14 | 2009-01-27 | Otsuka Pharmaceutical Co., Ltd. | Method for assay of antibodies and antibody assay device |
CA2548483A1 (en) * | 2003-08-26 | 2005-03-10 | Uwe Fiebig | Induction of antiviral neutralizing antibodies in humans and animals |
US8138318B2 (en) | 2007-09-13 | 2012-03-20 | Abbott Laboratories | Hepatitis B pre-S2 nucleic acid |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0290516A4 (de) * | 1986-10-24 | 1989-11-14 | Jeffrey A Whitsett | Proteine, die mit oberflächenwirksamen hydrophoben lungenwirkstoffen verbunden sind. |
-
1988
- 1988-11-23 US US07/276,263 patent/US5124255A/en not_active Expired - Lifetime
-
1989
- 1989-02-20 ES ES89102928T patent/ES2083957T3/es not_active Expired - Lifetime
- 1989-02-20 EP EP89102928A patent/EP0331961B1/de not_active Expired - Lifetime
- 1989-02-20 DE DE68925138T patent/DE68925138T2/de not_active Expired - Fee Related
- 1989-02-20 AT AT89102928T patent/ATE131876T1/de not_active IP Right Cessation
- 1989-03-10 JP JP1059466A patent/JPH0813273B2/ja not_active Expired - Fee Related
- 1989-03-10 KR KR1019890002942A patent/KR890014738A/ko not_active Application Discontinuation
- 1989-03-10 AU AU31206/89A patent/AU625554B2/en not_active Ceased
- 1989-03-10 CA CA000593373A patent/CA1335358C/en not_active Expired - Fee Related
-
1996
- 1996-01-17 GR GR960400099T patent/GR3018698T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
GR3018698T3 (en) | 1996-04-30 |
US5124255A (en) | 1992-06-23 |
ES2083957T3 (es) | 1996-05-01 |
EP0331961B1 (de) | 1995-12-20 |
ATE131876T1 (de) | 1996-01-15 |
AU625554B2 (en) | 1992-07-16 |
EP0331961A3 (en) | 1990-07-04 |
CA1335358C (en) | 1995-04-25 |
JPH0235089A (ja) | 1990-02-05 |
AU3120689A (en) | 1989-09-14 |
EP0331961A2 (de) | 1989-09-13 |
KR890014738A (ko) | 1989-10-25 |
JPH0813273B2 (ja) | 1996-02-14 |
DE68925138D1 (de) | 1996-02-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
DE68925138D1 (de) | Verfahren zur Herstellung von Proteinen durch Verwendung von CKS-Fusionsproteinen | |
DE68919246T2 (de) | Verfahren zur Herstellung von Aminosäuren. | |
DE58902544D1 (de) | Verfahren zur herstellung von 4-alkoxy-2-hydroxybenzophenon-5-sulfonsaeuren. | |
ATE81339T1 (de) | Verfahren zur herstellung von zyklischen sulfaten. | |
DE69431799T2 (de) | Verfahren zur erzeugung von russen | |
DE69011880D1 (de) | Verfahren zur Herstellung von L-Aminosäuren durch Fermentation. | |
ATE220714T1 (de) | Verfahren zur herstellung von proteinen | |
ATE218131T1 (de) | Verfahren zur herstellung von mono-n- substituierten tetraazamakrocyclen | |
ATA163789A (de) | Verfahren zur herstellung von keramischen formteilen | |
DE68919707D1 (de) | Verfahren zur erzeugung von unterchloriger säure. | |
DE68926922T2 (de) | Verfahren zur Herstellung von L-alpha-Aminosäuren | |
DE3774954D1 (de) | Verfahren zur herstellung von penemen. | |
AT384222B (de) | Verfahren zur herstellung von 7-amino-3alkoxymethyl-3-cephem-4-carbonsaeuren | |
DE3876917T2 (de) | Verfahren zur erzeugung von dimethylnaphthalenen. | |
ATE125298T1 (de) | Verfahren zur herstellung von motilinähnlichen polypeptiden und seine expression. | |
DE3872007T2 (de) | Verfahren zur herstellung von carbonsaeuren. | |
DE3882413T2 (de) | L-Valin produzierender Mikroorganismus und ein Verfahren zur Herstellung von L-Valin durch Gärung. | |
ATE139999T1 (de) | Verfahren zur herstellung von fluormethyl- substituierter piperidin-carbodithioaten | |
DE68916805T2 (de) | Verfahren zur herstellung von tocopherolen. | |
ATE120748T1 (de) | Verfahren zur herstellung von 2-acylamino-9-acyl- 6-halogen-purinen. | |
DE69606679T2 (de) | Verfahren zur Herstellung von Aryl- und Vinylnitrilen | |
DE3868160D1 (de) | Verfahren zur herstellung von carbonsaeuren. | |
ATE193284T1 (de) | Verfahren zur herstellung von benzylsubstituierten rhodaninederivaten | |
ATE92108T1 (de) | Verfahren zur herstellung von l-phosphinothricin durch transaminierung. | |
ATE82243T1 (de) | Verfahren zur herstellung von propinol. |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8339 | Ceased/non-payment of the annual fee |