ES2083957T3 - Metodo para la produccion de proteinas utilizando proteinas fusionadas del tipo cks. - Google Patents
Metodo para la produccion de proteinas utilizando proteinas fusionadas del tipo cks.Info
- Publication number
- ES2083957T3 ES2083957T3 ES89102928T ES89102928T ES2083957T3 ES 2083957 T3 ES2083957 T3 ES 2083957T3 ES 89102928 T ES89102928 T ES 89102928T ES 89102928 T ES89102928 T ES 89102928T ES 2083957 T3 ES2083957 T3 ES 2083957T3
- Authority
- ES
- Spain
- Prior art keywords
- proteins
- cks
- production
- type
- fused
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56983—Viruses
- G01N33/56988—HIV or HTLV
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
- G01N33/5767—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis non-A, non-B hepatitis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/033—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the internal surface of the plasma membrane, e.g. containing a myristoylation motif
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/33—Fusion polypeptide fusions for targeting to specific cell types, e.g. tissue specific targeting, targeting of a bacterial subspecies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16611—Simplexvirus, e.g. human herpesvirus 1, 2
- C12N2710/16622—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/16011—Human Immunodeficiency Virus, HIV
- C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
- C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Urology & Nephrology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Biophysics (AREA)
- Virology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Plant Pathology (AREA)
- Communicable Diseases (AREA)
- Toxicology (AREA)
- AIDS & HIV (AREA)
- Tropical Medicine & Parasitology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
ES DESCUBIERTO UN METODO DE PRODUCIR LAS PROTEINAS DE FUSION DONDE UNA PARTE DE LA PROTEINA DE FUSION ES FORMADA DE LA PROTEINA BACTERIAL CKS.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US16706788A | 1988-03-11 | 1988-03-11 | |
US07/276,263 US5124255A (en) | 1988-03-11 | 1988-11-23 | CKS method of protein synthesis |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2083957T3 true ES2083957T3 (es) | 1996-05-01 |
Family
ID=22605797
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES89102928T Expired - Lifetime ES2083957T3 (es) | 1988-03-11 | 1989-02-20 | Metodo para la produccion de proteinas utilizando proteinas fusionadas del tipo cks. |
Country Status (10)
Country | Link |
---|---|
US (1) | US5124255A (es) |
EP (1) | EP0331961B1 (es) |
JP (1) | JPH0813273B2 (es) |
KR (1) | KR890014738A (es) |
AT (1) | ATE131876T1 (es) |
AU (1) | AU625554B2 (es) |
CA (1) | CA1335358C (es) |
DE (1) | DE68925138T2 (es) |
ES (1) | ES2083957T3 (es) |
GR (1) | GR3018698T3 (es) |
Families Citing this family (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU650038B2 (en) * | 1988-03-11 | 1994-06-09 | Abbott Laboratories | CKS method of protein synthesis |
US5312737A (en) * | 1988-03-11 | 1994-05-17 | Abbott Laboratories | CKS method of HCV protein synthesis |
CA2003383A1 (en) * | 1988-11-23 | 1990-05-23 | Sushil G. Devare | Synthetic dna derived recombinant hiv antigens |
WO1990013652A1 (en) * | 1989-05-12 | 1990-11-15 | Triton Diagnostics, Inc. | Herpes simplex virus type 2-glycoprotein g proteins and polypeptides |
DE4002636A1 (de) * | 1990-01-30 | 1991-08-01 | Boehringer Mannheim Gmbh | Expression von hiv1- und 2-polypeptiden und deren verwendung |
US6172189B1 (en) * | 1990-08-24 | 2001-01-09 | Abbott Laboratories | Hepatitis C assay utilizing recombinant antigens |
CA2049679C (en) * | 1990-08-24 | 2005-06-21 | Sushil G. Devare | Hepatitis c assay utilizing recombinant antigens |
ES2132115T3 (es) | 1990-11-07 | 1999-08-16 | Abbott Lab | Anticuerpos monoclonales contra el virus de la hepatitis c y su procedimiento de utilizacion. |
CA2115926A1 (en) * | 1991-08-21 | 1993-03-04 | Stephen H. Dailey | Hepatitis c assay utilizing recombinant antigens to ns1 |
AU2492792A (en) * | 1991-08-21 | 1993-03-16 | Abbott Laboratories | Hepatitis c assay utilizing recombinant antigens from ns5 region |
US5965702A (en) * | 1991-10-21 | 1999-10-12 | Abbott Laboratories | Borrelia burgdorferi antigens and uses thereof |
WO1993025533A1 (en) * | 1992-06-05 | 1993-12-23 | Abbott Laboratories | Methods and reagents for the determination of immunosuppressive agents |
US5607914A (en) * | 1993-01-13 | 1997-03-04 | Pioneer Hi-Bred International, Inc. | Synthetic antimicrobial peptides |
CA2125467C (en) * | 1993-07-06 | 2001-02-06 | Heinz Dobeli | Process for producing hydrophobic polypeptides, proteins or peptides |
DE4405810A1 (de) | 1994-02-23 | 1995-08-24 | Behringwerke Ag | Von einem Retrovirus aus der HIV-Gruppe abgeleitete Peptide und deren Verwendung |
US6511812B1 (en) * | 1994-05-09 | 2003-01-28 | Abbott Laboratories | Method and test kit for use in improving immunoassay specificity |
JPH10504098A (ja) * | 1994-05-09 | 1998-04-14 | アボツト・ラボラトリーズ | イムノアッセイの特異性の改良に有用な方法及び試薬 |
IT1266740B1 (it) * | 1994-07-01 | 1997-01-14 | Maria Paola Landini | Materiale proteico ricombinante legante anticorpi contro il citomegalovirus umano, reagenti diagnostici derivati da tale |
CA2221192C (en) * | 1995-05-15 | 2011-11-01 | Abbott Laboratories | Embedded expression of heterologous proteins |
US7901910B2 (en) | 1996-05-14 | 2011-03-08 | Gregory Thomas Maine | Embedded expression of insoluble heterologous proteins |
IT1290804B1 (it) | 1996-08-16 | 1998-12-11 | Abbott Lab | Saggio western blot perfezionato per l'individuazione di anticorpi specifici contro il virus hcmv |
US20050202499A1 (en) | 1996-10-31 | 2005-09-15 | Billing-Medel Patricia A. | Reagents and methods useful for detecting diseases of the breast |
US5922533A (en) * | 1997-08-15 | 1999-07-13 | Abbott Laboratories | Rapid assay for simultaneous detection and differentiation of antibodies to HIV groups |
US6846905B2 (en) | 1997-08-15 | 2005-01-25 | Abbott Laboratories | Antigen constructs useful in the detection and differentiation of antibodies to HIV |
CA2293677C (en) * | 1998-04-14 | 2009-01-27 | Otsuka Pharmaceutical Co., Ltd. | Method for assay of antibodies and antibody assay device |
CA2548483A1 (en) * | 2003-08-26 | 2005-03-10 | Uwe Fiebig | Induction of antiviral neutralizing antibodies in humans and animals |
US8138318B2 (en) | 2007-09-13 | 2012-03-20 | Abbott Laboratories | Hepatitis B pre-S2 nucleic acid |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0290516A4 (en) * | 1986-10-24 | 1989-11-14 | Jeffrey A Whitsett | PROTEINS RELATED TO SURFACE-EFFECTIVE HYDROPHOBIC LUNG ACTIVE SUBSTANCES. |
-
1988
- 1988-11-23 US US07/276,263 patent/US5124255A/en not_active Expired - Lifetime
-
1989
- 1989-02-20 ES ES89102928T patent/ES2083957T3/es not_active Expired - Lifetime
- 1989-02-20 EP EP89102928A patent/EP0331961B1/en not_active Expired - Lifetime
- 1989-02-20 DE DE68925138T patent/DE68925138T2/de not_active Expired - Fee Related
- 1989-02-20 AT AT89102928T patent/ATE131876T1/de not_active IP Right Cessation
- 1989-03-10 AU AU31206/89A patent/AU625554B2/en not_active Ceased
- 1989-03-10 JP JP1059466A patent/JPH0813273B2/ja not_active Expired - Fee Related
- 1989-03-10 KR KR1019890002942A patent/KR890014738A/ko not_active Application Discontinuation
- 1989-03-10 CA CA000593373A patent/CA1335358C/en not_active Expired - Fee Related
-
1996
- 1996-01-17 GR GR960400099T patent/GR3018698T3/el unknown
Also Published As
Publication number | Publication date |
---|---|
AU625554B2 (en) | 1992-07-16 |
EP0331961A2 (en) | 1989-09-13 |
ATE131876T1 (de) | 1996-01-15 |
CA1335358C (en) | 1995-04-25 |
DE68925138T2 (de) | 1996-06-13 |
JPH0813273B2 (ja) | 1996-02-14 |
KR890014738A (ko) | 1989-10-25 |
EP0331961A3 (en) | 1990-07-04 |
GR3018698T3 (en) | 1996-04-30 |
DE68925138D1 (de) | 1996-02-01 |
US5124255A (en) | 1992-06-23 |
EP0331961B1 (en) | 1995-12-20 |
JPH0235089A (ja) | 1990-02-05 |
AU3120689A (en) | 1989-09-14 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG2A | Definitive protection |
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