DE674710C - Process for the extraction of substances that lower blood pressure - Google Patents
Process for the extraction of substances that lower blood pressureInfo
- Publication number
- DE674710C DE674710C DEB180451D DEB0180451D DE674710C DE 674710 C DE674710 C DE 674710C DE B180451 D DEB180451 D DE B180451D DE B0180451 D DEB0180451 D DE B0180451D DE 674710 C DE674710 C DE 674710C
- Authority
- DE
- Germany
- Prior art keywords
- substances
- blood pressure
- urine
- extraction
- lower blood
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/22—Urine; Urinary tract, e.g. kidney or bladder; Intraglomerular mesangial cells; Renal mesenchymal cells; Adrenal gland
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Cell Biology (AREA)
- Urology & Nephrology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Immunology (AREA)
- Virology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Description
Verfahren zur Gewinnung von blutdrucksenkenden Stoffen Es ist bekannt, daß der Harn normaler Menschen und Tiere blutdrucksenkende Stoffe enthält, deren chemische Zusammensetzung allerdings noch nicht genau ermittelt werden konnte. Im weiteren ist experimentell nachgewiesen worden, daß durch Eninervung der Niere bei Hundeneine Herabsetzung des Blutdruckes erzielt wird.Process for obtaining substances that lower blood pressure It is known that the urine of normal people and animals contains substances that lower blood pressure, their chemical composition could not yet be determined exactly. in the further it has been experimentally proven that by enervation of the kidney Dogs a reduction in blood pressure is achieved.
Es -wurde die überraschende Beobachtung gemacht, daß dabei eine Vermehrung der blutdrucksenkenden Stoffe im Harn eintritt. Diese Vermehrung der blutdrucksenkenden Stoffe war nicht zu erwarten, weil es wahrscheinlicher war, daß die Entnervungunmittelbar auf die Gefäße der Niere wirke, da ja der Einfluß der Nerven auf die Steueruhdes Blutkreislaufes . bekannt ist. Die blutdrucksenkenden Stoffe können aus dem Harn in an sich bekannter Weise gewonnen -werden.The surprising observation was made that there was an increase in blood pressure lowering substances in the urine. This increase in blood pressure lowering substances was not to be expected, because it was more likely that the nervous system would act directly on the vessels of the kidney, since the nerves had an influence on the control of the blood circulation . is known. The blood pressure lowering substances can be obtained from the urine in a manner known per se.
Für das vorliegende Verfahren können verschiedene Tiere Verwendung finden. Insbesondere eignen sich Säugetiere, wie Hunde, Pferde, Kühe usw.Various animals can be used for the present method Find. Mammals such as dogs, horses, cows, etc. are particularly suitable.
Die Gewinnung der blutdrucksenkenden Stoffe aus dem Harn kann z. B. durch Adsorption, durch Extraktion oder auch durch Ausfällen vorgenommen werden. Die Verfahrens-Stoffe sollen therapeutische Verwendung finden.The extraction of the blood pressure lowering substances from the urine can z. B. be carried out by adsorption, by extraction or by precipitation. The process substances are intended to be used therapeutically.
Beispiel Ein Hund im Gewicht von etwa 15 kg wird in Äthernarkose folgender Operation unterworfen: Nach öffnung der Bauchhöhle wird nach Beis#eiteschieben des Netzes und der Dünndarmschlingen die Niere aufgesucht, stumpf losgelöst und vorgelagert. Die Arteria renalis (Nierenschlagader) wird, soweit als möglich, frei präpariert und hierauf mit SO/oi,-er Phenollösung gepinselt. Dadurch werden die vorzugsweise in der Adventitia (Außenschicht) verlaufenden Nervenstäminchen, die sonst nur schwer zu erkennen sind, deutlicher sichtbar und hierauf in einer möglichst großen Ausdehnung vorsichtig reseziert, ebenso sonstige im Hilus verlaufende Nervenfasern. Die Niete wird hierauf wieder in ihr Lager gebracht und die Bauchwand durch Knopfnähte schichtenweise verschlossen.Example A dog weighing about 15 kg is subjected to the following operation under ether anesthesia: After opening the abdominal cavity, after pushing aside the net and the small intestine loops, the kidney is sought out, bluntly detached and placed in front. The renal artery (renal artery) is, as far as possible, freely dissected and then brushed with a phenol solution. As a result, the small nerve stems that are preferably in the adventitia (outer layer), which are otherwise difficult to see, are more clearly visible and are then carefully resected over the largest possible extent, as are other nerve fibers that run in the hilus. The rivet is then returned to its bearing and the abdominal wall is closed in layers with button sutures.
Falls doppelseitig entnervt wird, erfolgt die Operation der zweiten Seite ungefähr i Woche nach der ersten Operation in gleicher Weise.If both sides are unnerved, the second operation is carried out Page about one week after the first operation in the same way.
Zur Harngewinnung werden die flunde frühestens i Woche nach der Operation für 2 Tage in einen Stoffwechselkäfig gegeben, um die ganze Harnmenge sammeln zu können. Die zweitägige Harnmenge, im Durchschnitt ungefähr 11, wird mit einigen Tropfen Chloroform versetzt, um Zersetzung züi verhindern. Zur Darstellung der wirksamen Stoffe wird der Harn mit einem Adsorptionsmittel, wie Aluminiumhydroxyd, Kaolin, Tonerde, Fullererde, Tierkohle u. dgl., versetzt und etwa i Stunde kräftig durchgeschüttelt. Hierauf wird das Gemisch i Tag lang stehengelassen, sodann das Adsorbat abfiltriert, bei etwa 40' ,O*etrocknet, fein gepulvert und mit Aceton ausgezogen. Der nach dem Verdampfen des Auszuges erhaltene gelbbraune Trockenrückstand enthält blutdrucksenkende Stoffe.To obtain urine, the drops are placed in a metabolic cage for 2 days at the earliest one week after the operation so that the entire amount of urine can be collected. The two-day urine volume, on average, about 1 1, is treated with a few drops of chloroform, züi prevent decomposition. To display the active substances, the urine is mixed with an adsorbent such as aluminum hydroxide, kaolin, clay, fuller's earth, animal charcoal and the like, and shaken vigorously for about an hour. The mixture is then left to stand for a day, then the adsorbate is filtered off, dried at about 40 ', dried, finely powdered and extracted with acetone. The yellow-brown dry residue obtained after evaporation of the extract contains substances that lower blood pressure.
Zur pharmakologisch6n Prüfung wird der Rückstand in Wasser aufgenommen, die wässerige Lösung mit Sodalösung schwach alkalisch gemacht und zur Konservierung etwas Trikresollösung hinzugefügt.For pharmacological testing, the residue is taken up in water, the aqueous solution made weakly alkaline with soda solution and used for preservation added some tricresol solution.
Man kann die blutdrucksenkenden Stoffe auch durch Ausziehen des Harns mit wasserunlöslichen Lösungsmitteln, z. B. höheren Al- koholen, oder auch durch Ausfällen gewinnen.The antihypertensive substances can also be removed by drawing out the urine with water-insoluble solvents, e.g. B. koholen higher Al, or win by precipitation.
Claims (1)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CH674710X | 1937-10-22 |
Publications (1)
Publication Number | Publication Date |
---|---|
DE674710C true DE674710C (en) | 1939-04-21 |
Family
ID=4527992
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DEB180451D Expired DE674710C (en) | 1937-10-22 | 1937-10-24 | Process for the extraction of substances that lower blood pressure |
Country Status (1)
Country | Link |
---|---|
DE (1) | DE674710C (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1017744B (en) * | 1954-04-27 | 1957-10-17 | Jaeger Karl Heinz | Process for obtaining growth-promoting substances for therapeutic purposes |
DE1092609B (en) * | 1956-07-17 | 1960-11-10 | Paul Niehans Dr Med | Process for the production of diagnostically and therapeutically applicable active substances |
-
1937
- 1937-10-24 DE DEB180451D patent/DE674710C/en not_active Expired
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE1017744B (en) * | 1954-04-27 | 1957-10-17 | Jaeger Karl Heinz | Process for obtaining growth-promoting substances for therapeutic purposes |
DE1092609B (en) * | 1956-07-17 | 1960-11-10 | Paul Niehans Dr Med | Process for the production of diagnostically and therapeutically applicable active substances |
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