DE60336228D1 - Verfahren zur gleichzeitigen herstellung von multiplen proteinen; vektoren und zellen, die hierfür verwendung finden - Google Patents

Verfahren zur gleichzeitigen herstellung von multiplen proteinen; vektoren und zellen, die hierfür verwendung finden

Info

Publication number
DE60336228D1
DE60336228D1 DE60336228T DE60336228T DE60336228D1 DE 60336228 D1 DE60336228 D1 DE 60336228D1 DE 60336228 T DE60336228 T DE 60336228T DE 60336228 T DE60336228 T DE 60336228T DE 60336228 D1 DE60336228 D1 DE 60336228D1
Authority
DE
Germany
Prior art keywords
proteins
cells
vectors
apply
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60336228T
Other languages
German (de)
English (en)
Inventor
Arie Pieter Otte
Arthur Leo Kruckeberg
Richard George Sewalt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chromagenics BV
Original Assignee
Chromagenics BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chromagenics BV filed Critical Chromagenics BV
Application granted granted Critical
Publication of DE60336228D1 publication Critical patent/DE60336228D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/822—Reducing position variability, e.g. by the use of scaffold attachment region/matrix attachment region (SAR/MAR); Use of SAR/MAR to regulate gene expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE60336228T 2002-06-14 2003-06-13 Verfahren zur gleichzeitigen herstellung von multiplen proteinen; vektoren und zellen, die hierfür verwendung finden Expired - Lifetime DE60336228D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP02077350 2002-06-14
PCT/NL2003/000432 WO2003106684A2 (en) 2002-06-14 2003-06-13 A method for simultaneous production of multiple proteins; vectors and cells for use therein

Publications (1)

Publication Number Publication Date
DE60336228D1 true DE60336228D1 (de) 2011-04-14

Family

ID=29724494

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60336228T Expired - Lifetime DE60336228D1 (de) 2002-06-14 2003-06-13 Verfahren zur gleichzeitigen herstellung von multiplen proteinen; vektoren und zellen, die hierfür verwendung finden

Country Status (11)

Country Link
US (3) US7364878B2 (https=)
EP (1) EP1513937B1 (https=)
JP (3) JP4647309B2 (https=)
AT (1) ATE500331T1 (https=)
AU (1) AU2003238719B2 (https=)
CA (3) CA2489475C (https=)
DE (1) DE60336228D1 (https=)
DK (1) DK1513937T3 (https=)
ES (1) ES2362273T3 (https=)
NZ (1) NZ537073A (https=)
WO (1) WO2003106684A2 (https=)

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ATE466941T1 (de) * 2001-07-04 2010-05-15 Chromagenics Bv Dns-sequenzen mit anti-repressor-aktivität
CA2489475C (en) 2002-06-14 2011-03-29 Chromagenics B.V. A method for simultaneous production of multiple proteins; vectors and cells for use therein
AU2003243059A1 (en) * 2002-06-14 2003-12-31 Chromagenics B.V. Use of repression blocking sequences in methods for enhancing gene expression
NZ537277A (en) 2002-07-18 2008-04-30 Crucell Holland Bv Recombinant production of mixtures of antibodies
USRE47770E1 (en) 2002-07-18 2019-12-17 Merus N.V. Recombinant production of mixtures of antibodies
EP1573058B1 (en) * 2002-12-18 2011-09-14 ChromaGenics B.V. A method for improving protein production
ATE354645T1 (de) 2002-12-20 2007-03-15 Chromagenics Bv Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
ES2408582T3 (es) 2003-05-30 2013-06-21 Merus B.V. Biblioteca de Fab para la preparación de una mezcla de anticuerpos
US20100069614A1 (en) 2008-06-27 2010-03-18 Merus B.V. Antibody producing non-human mammals
DK1737971T3 (da) 2004-01-20 2017-11-13 Merus Nv Blandinger af bindingsproteiner
PT1763586E (pt) * 2004-07-08 2008-02-25 Chromagenics Bv Nova sequência para melhorar a expressão de ácido nucleico
US20100136616A1 (en) * 2004-11-08 2010-06-03 Chromagenics B.V. Selection of Host Cells Expressing Protein at High Levels
US20060172382A1 (en) * 2004-11-08 2006-08-03 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
EP1809750B1 (en) * 2004-11-08 2012-03-21 ChromaGenics B.V. Selection of host cells expressing protein at high levels
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20070042397A1 (en) * 2005-03-03 2007-02-22 International Business Machines Corporation Techniques for linking non-coding and gene-coding deoxyribonucleic acid sequences and applications thereof
PT1987150E (pt) * 2006-02-21 2011-09-08 Chromagenics Bv Selecção de células hospedeiras expressando níveis elevados de proteína
US20080293086A1 (en) * 2006-09-18 2008-11-27 Cobalt Technologies, Inc. A Delaware Corporation Real time monitoring of microbial enzymatic pathways
JP2010504507A (ja) * 2006-09-20 2010-02-12 インスティチュート・パスツール・コリア 細胞におけるターゲットタンパク質候補の発現を検出および／または定量する方法、ならびに小分子モデュレーターのターゲットタンパク質を同定する方法
US20090081715A1 (en) * 2007-09-07 2009-03-26 Cobalt Technologies, Inc., A Delaware Corporation Engineered Light-Emitting Reporter Genes
WO2009126795A2 (en) 2008-04-09 2009-10-15 Cobalt Technologies, Inc. Immobilized product tolerant microorganisms
EP2443239B1 (en) 2009-06-15 2015-11-25 Cellagenics B.V. Novel stringent selectable markers
RU2011153546A (ru) * 2009-06-26 2013-08-10 Кобальт Текнолоджиз, Инк. Способ и комплексная система для получения биопродукта
CN103038352B (zh) 2010-06-15 2015-12-16 萨拉基尼克有限公司 用于增强基因表达的新型基因间元件
CN103180443B (zh) 2010-09-01 2016-01-13 萨拉基尼克有限公司 用于增强基因表达的来自核糖体蛋白启动子的核酸片段
US9617329B2 (en) 2010-10-01 2017-04-11 Csl Limited Method of cloning nucleic acid
WO2013078433A1 (en) 2011-11-23 2013-05-30 University Of Hawaii Auto-processing domains for polypeptide expression
SI2838917T1 (sl) 2012-04-20 2019-11-29 Merus Nv Postopki in sredstva za produkcijo heterodimernih IG-podobnih molekul
JP6343011B2 (ja) * 2013-10-07 2018-06-13 プレステージ バイオファーマ プライベート リミテッド 抗体発現用バイシストロニック発現ベクター及びそれを用いた抗体の生産方法
US20260041746A1 (en) * 2022-08-09 2026-02-12 Geg Tech Transient expression system for rna, for vaccination
EP4569107A1 (en) * 2022-08-09 2025-06-18 GEG Tech Transient expression system for rna, for gene editing
EP4569106A1 (en) * 2022-08-09 2025-06-18 GEG Tech Transient expression system for rna, for cosmetic uses
WO2024033441A1 (en) * 2022-08-09 2024-02-15 Geg Tech Transient expression system for rna

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AU2003243059A1 (en) * 2002-06-14 2003-12-31 Chromagenics B.V. Use of repression blocking sequences in methods for enhancing gene expression
CA2489475C (en) 2002-06-14 2011-03-29 Chromagenics B.V. A method for simultaneous production of multiple proteins; vectors and cells for use therein
EP1573058B1 (en) 2002-12-18 2011-09-14 ChromaGenics B.V. A method for improving protein production
ATE354645T1 (de) 2002-12-20 2007-03-15 Chromagenics Bv Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
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Also Published As

Publication number Publication date
CA2489475A1 (en) 2003-12-24
CA2722998A1 (en) 2003-12-24
JP5095690B2 (ja) 2012-12-12
US7901908B2 (en) 2011-03-08
ATE500331T1 (de) 2011-03-15
US20080227199A1 (en) 2008-09-18
US20080227151A1 (en) 2008-09-18
WO2003106684A2 (en) 2003-12-24
AU2003238719A1 (en) 2003-12-31
CA2723500C (en) 2014-10-28
JP2005529610A (ja) 2005-10-06
US7364878B2 (en) 2008-04-29
JP5095693B2 (ja) 2012-12-12
AU2003238719B2 (en) 2008-02-21
CA2723500A1 (en) 2003-12-24
CA2489475C (en) 2011-03-29
US20050191723A1 (en) 2005-09-01
WO2003106684A3 (en) 2004-04-08
JP2010004886A (ja) 2010-01-14
NZ537073A (en) 2006-08-31
EP1513937A2 (en) 2005-03-16
JP2010011859A (ja) 2010-01-21
EP1513937B1 (en) 2011-03-02
CA2722998C (en) 2014-12-02
US7960143B2 (en) 2011-06-14
JP4647309B2 (ja) 2011-03-09
DK1513937T3 (da) 2011-05-16
ES2362273T3 (es) 2011-06-30

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