DE60335649D1 - Universeller tag-assay - Google Patents

Universeller tag-assay

Info

Publication number
DE60335649D1
DE60335649D1 DE60335649T DE60335649T DE60335649D1 DE 60335649 D1 DE60335649 D1 DE 60335649D1 DE 60335649 T DE60335649 T DE 60335649T DE 60335649 T DE60335649 T DE 60335649T DE 60335649 D1 DE60335649 D1 DE 60335649D1
Authority
DE
Germany
Prior art keywords
universal
detection probes
identifier
target
tag
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60335649T
Other languages
English (en)
Inventor
Donald M Crothers
Erik R Holmlin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GeneOhm Sciences Inc
Original Assignee
GeneOhm Sciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GeneOhm Sciences Inc filed Critical GeneOhm Sciences Inc
Application granted granted Critical
Publication of DE60335649D1 publication Critical patent/DE60335649D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DE60335649T 2002-11-06 2003-11-05 Universeller tag-assay Expired - Lifetime DE60335649D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US42465602P 2002-11-06 2002-11-06
US10/424,542 US20040086892A1 (en) 2002-11-06 2003-04-24 Universal tag assay
PCT/US2003/035378 WO2004044549A2 (en) 2002-11-06 2003-11-05 Universal tag assay

Publications (1)

Publication Number Publication Date
DE60335649D1 true DE60335649D1 (de) 2011-02-17

Family

ID=32180019

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60335649T Expired - Lifetime DE60335649D1 (de) 2002-11-06 2003-11-05 Universeller tag-assay

Country Status (8)

Country Link
US (1) US20040086892A1 (de)
EP (1) EP1558764B1 (de)
JP (1) JP4505414B2 (de)
AT (1) ATE494389T1 (de)
AU (1) AU2003287533A1 (de)
CA (1) CA2505254C (de)
DE (1) DE60335649D1 (de)
WO (1) WO2004044549A2 (de)

Families Citing this family (92)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0205455D0 (en) 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US20050118616A1 (en) * 2002-08-16 2005-06-02 Kawashima Tadashi R. Amplification of target nucleotide sequence without polymerase chain reaction
US7258978B2 (en) * 2002-11-06 2007-08-21 Geneohm Sciences Electrochemical method to measure DNA attachment to an electrode surface in the presence of molecular oxygen
US20040086895A1 (en) * 2002-11-06 2004-05-06 Crothers Donald M. Method of electrochemical detection of somatic cell mutations
US20070099194A1 (en) * 2005-11-02 2007-05-03 Xing Yang Quality control of probe attachment on electrodes
US10533998B2 (en) 2008-07-18 2020-01-14 Bio-Rad Laboratories, Inc. Enzyme quantification
ATE456678T1 (de) * 2003-11-10 2010-02-15 Geneohm Sciences Inc Nukleinsäurenachweisverfahren mit erhöhter empfindlichkeit
US7341834B2 (en) 2003-12-15 2008-03-11 Geneohn Sciences, Inc. Multiplexed electrochemical detection system and method
DE602004023875D1 (de) 2003-12-15 2009-12-10 Geneohm Sciences Inc Kohlenstoffelektrodenoberfläche zur bindung von dna- und proteinmolekülen daran
SE0401270D0 (sv) * 2004-05-18 2004-05-18 Fredrik Dahl Method for amplifying specific nucleic acids in parallel
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
WO2006065598A2 (en) * 2004-12-13 2006-06-22 Geneohm Sciences, Inc. Fluidic cartridges for electrochemical detection of dna
GB0502010D0 (en) * 2005-02-01 2005-03-09 Enigma Diagnostics Ltd Biochemical reagents and their uses
EP1871896B1 (de) * 2005-04-12 2015-06-03 Olink AB Zirkulare Sonden und deren Verwendung zur Identifizierung von Biomolekülen
WO2006108423A2 (en) * 2005-04-12 2006-10-19 In Situ Rcp A/S Under Founding Methods for production of oligonucleotides
DK1907583T4 (da) 2005-06-15 2020-01-27 Complete Genomics Inc Enkeltmolekyle-arrays til genetisk og kemisk analyse
US20090117552A1 (en) * 2005-07-07 2009-05-07 Pamgene Bv Method for Detection and Quantification of Target Nucleic Acids in a Sample
US7960104B2 (en) * 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
US8986926B2 (en) * 2005-12-23 2015-03-24 Nanostring Technologies, Inc. Compositions comprising oriented, immobilized macromolecules and methods for their preparation
EP1984738A2 (de) 2006-01-11 2008-10-29 Raindance Technologies, Inc. Mikrofluidische vorrichtungen und verfahren zur verwendung bei der bildung und kontrolle von nanoreaktoren
JP4989493B2 (ja) * 2006-01-20 2012-08-01 オリンパス株式会社 分子内プローブによる核酸配列の検出方法
WO2007092538A2 (en) 2006-02-07 2007-08-16 President And Fellows Of Harvard College Methods for making nucleotide probes for sequencing and synthesis
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP2530167A1 (de) 2006-05-11 2012-12-05 Raindance Technologies, Inc. Mikrofluidische Vorrichtungen
JP2009536825A (ja) * 2006-05-11 2009-10-22 ジーンオーム サイエンシーズ、インク． 可変ゲノムのための１００％配列一致検出方法
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
WO2008097559A2 (en) 2007-02-06 2008-08-14 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US9551026B2 (en) * 2007-12-03 2017-01-24 Complete Genomincs, Inc. Method for nucleic acid detection using voltage enhancement
EP2315629B1 (de) 2008-07-18 2021-12-15 Bio-Rad Laboratories, Inc. Tröpfchenbibliotheken
US8697363B2 (en) * 2008-08-26 2014-04-15 Fluidigm Corporation Methods for detecting multiple target nucleic acids in multiple samples by use nucleotide tags
DK2414547T3 (da) * 2009-04-02 2014-06-16 Fluidigm Corp Multiprimer-amplifikationsmetode til stregkodning af målnukleinsyrer
US20130296189A1 (en) * 2009-05-08 2013-11-07 Suzhou Institute of Nano-tech and Nano-bionics, Chinese Academy of Sciences Probes utilizing universal tags, a kit comprising the same and detection methods
JP5610258B2 (ja) * 2009-09-09 2014-10-22 国立大学法人 筑波大学 送液装置
AU2010315303B2 (en) * 2009-11-03 2015-08-06 Htg Molecular Diagnostics, Inc. Quantitative Nuclease Protection Sequencing (qNPS)
EP3597771A1 (de) * 2009-11-25 2020-01-22 Gen9, Inc. Verfahren und vorrichtung für chipbasierte dna-fehlerreduktion
CN102648295B (zh) * 2009-12-07 2017-08-08 伊鲁米那股份有限公司 用于多重基因分型的多样品索引
US9399797B2 (en) * 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
EP2534267B1 (de) 2010-02-12 2018-04-11 Raindance Technologies, Inc. Digitale analyse von analyten
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
EP3447155A1 (de) 2010-09-30 2019-02-27 Raindance Technologies, Inc. Sandwichassays in tröpfchen
EP3859011A1 (de) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Verfahren zur bildung gemischter tröpfchen
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
US8741564B2 (en) 2011-05-04 2014-06-03 Htg Molecular Diagnostics, Inc. Quantitative nuclease protection assay (QNPA) and sequencing (QNPS) improvements
WO2012162267A2 (en) 2011-05-20 2012-11-29 Fluidigm Corporation Nucleic acid encoding reactions
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
CA2848304A1 (en) 2011-09-09 2013-03-14 The Board Of Trustees Of The Leland Stanford Junior University Methods for sequencing a polynucleotide
US10837879B2 (en) 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
US9862994B2 (en) 2012-02-09 2018-01-09 Dana-Farber Cancer Institute, Inc. Selective nucleic acid amplification from nucleic acid pools
CA2874343C (en) 2012-05-21 2021-11-09 Fluidigm Corporation Single-particle analysis of particle populations
CN103014168A (zh) * 2012-12-28 2013-04-03 北京大学 基于DNA hairpin和RCA的核酸检测方法
AU2014290454A1 (en) 2013-07-18 2016-01-28 President And Fellows Of Harvard College Specific nucleic acid amplification with compounded selectivity
TWI646230B (zh) 2013-08-05 2019-01-01 扭轉生物科技有限公司 重新合成之基因庫
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US10752940B2 (en) * 2013-11-08 2020-08-25 Ionis Pharmaceuticals, Inc. Compounds and methods for detecting oligonucleotides
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
PT3889271T (pt) * 2014-06-06 2022-12-20 Univ Cornell Método para identificação e enumeração de alterações de sequência de ácidos nucleicos, expressão, cópia ou metilação de adn, utilizando reações de nuclease, ligase, polimerase e sequenciação combinadas
CA2975852A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
CA2975855A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Compositions and methods for synthetic gene assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
CA2998169A1 (en) 2015-09-18 2017-03-23 Twist Bioscience Corporation Oligonucleic acid variant libraries and synthesis thereof
CN108698012A (zh) 2015-09-22 2018-10-23 特韦斯特生物科学公司 用于核酸合成的柔性基底
EP3384077A4 (de) 2015-12-01 2019-05-08 Twist Bioscience Corporation Funktionalisierte oberflächen und herstellung davon
WO2017106777A1 (en) 2015-12-16 2017-06-22 Fluidigm Corporation High-level multiplex amplification
EP3419989A4 (de) 2016-02-26 2019-11-06 Alere San Diego, Inc. Redox-markierte oligonukleotidsonden und deren verwendung
JP7017246B2 (ja) * 2016-07-25 2022-02-08 国立大学法人群馬大学 遺伝子変異を有するポリヌクレオチド配列の簡便検出法
KR102212257B1 (ko) 2016-08-22 2021-02-04 트위스트 바이오사이언스 코포레이션 드 노보 합성된 핵산 라이브러리
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
JP7280181B2 (ja) * 2016-10-01 2023-05-23 バークレー ライツ，インコーポレイテッド Ｄｎａバーコード組成物及びマイクロ流体デバイスによるインサイチュ同定法
JP6346242B2 (ja) 2016-11-04 2018-06-20 株式会社東芝 核酸検出方法及びアッセイキット
US10907274B2 (en) 2016-12-16 2021-02-02 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
US11550939B2 (en) 2017-02-22 2023-01-10 Twist Bioscience Corporation Nucleic acid based data storage using enzymatic bioencryption
US10894959B2 (en) 2017-03-15 2021-01-19 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
EP3638782A4 (de) 2017-06-12 2021-03-17 Twist Bioscience Corporation Verfahren für nahtlose nukleinsäureanordnung
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
KR20240024357A (ko) 2017-10-20 2024-02-23 트위스트 바이오사이언스 코포레이션 폴리뉴클레오타이드 합성을 위한 가열된 나노웰
US10936953B2 (en) 2018-01-04 2021-03-02 Twist Bioscience Corporation DNA-based digital information storage with sidewall electrodes
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
EP3814497A4 (de) 2018-05-18 2022-03-02 Twist Bioscience Corporation Polynukleotide, reagenzien und verfahren zur nukleinsäurehybridisierung
US11608522B2 (en) 2018-06-01 2023-03-21 Korea University Research And Business Foundation Method of detecting target nucleic acid using rolling circle amplification and composition for detecting target nucleic acid
WO2020028194A1 (en) 2018-07-30 2020-02-06 Readcoor, Inc. Methods and systems for sample processing or analysis
BR112021002189A2 (pt) 2018-08-08 2021-05-04 Inivata Ltd. método de sequenciamento que usa pcr multiplex de replicação variável
KR20210043605A (ko) * 2018-08-14 2021-04-21 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산의 체외 검출
US11492727B2 (en) 2019-02-26 2022-11-08 Twist Bioscience Corporation Variant nucleic acid libraries for GLP1 receptor
SG11202109283UA (en) 2019-02-26 2021-09-29 Twist Bioscience Corp Variant nucleic acid libraries for antibody optimization
EP3987019A4 (de) 2019-06-21 2023-04-19 Twist Bioscience Corporation Barcode-basierte nukleinsäuresequenzanordnung
WO2022056347A1 (en) * 2020-09-14 2022-03-17 Mayo Foundation For Medical Education And Research Probabilistic inference for estimating tonic concentrations using multiple cyclic square wave voltammetry
CN112288060A (zh) * 2020-11-09 2021-01-29 北京沃东天骏信息技术有限公司 用于识别标签的方法和装置
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU583258B2 (en) * 1983-12-16 1989-04-27 Medisense Inc. Assay for nucleic acids
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) * 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US4987071A (en) * 1986-12-03 1991-01-22 University Patents, Inc. RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5246921A (en) * 1990-06-26 1993-09-21 The Wistar Institute Of Anatomy And Biology Method for treating leukemias
US5776672A (en) * 1990-09-28 1998-07-07 Kabushiki Kaisha Toshiba Gene detection method
US5605662A (en) * 1993-11-01 1997-02-25 Nanogen, Inc. Active programmable electronic devices for molecular biological analysis and diagnostics
US5384261A (en) * 1991-11-22 1995-01-24 Affymax Technologies N.V. Very large scale immobilized polymer synthesis using mechanically directed flow paths
US5412087A (en) * 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
US5312527A (en) * 1992-10-06 1994-05-17 Concordia University Voltammetric sequence-selective sensor for target polynucleotide sequences
US6096880A (en) * 1993-04-15 2000-08-01 University Of Rochester Circular DNA vectors for synthesis of RNA and DNA
US5714320A (en) * 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
US5952172A (en) * 1993-12-10 1999-09-14 California Institute Of Technology Nucleic acid mediated electron transfer
SE9400522D0 (sv) * 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
US5871918A (en) * 1996-06-20 1999-02-16 The University Of North Carolina At Chapel Hill Electrochemical detection of nucleic acid hybridization
US5942391A (en) * 1994-06-22 1999-08-24 Mount Sinai School Of Medicine Nucleic acid amplification method: ramification-extension amplification method (RAM)
US5876924A (en) * 1994-06-22 1999-03-02 Mount Sinai School Of Medicine Nucleic acid amplification method hybridization signal amplification method (HSAM)
US5604097A (en) * 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
US5695934A (en) * 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
US5846719A (en) * 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US6207369B1 (en) * 1995-03-10 2001-03-27 Meso Scale Technologies, Llc Multi-array, multi-specific electrochemiluminescence testing
US6180346B1 (en) * 1995-06-27 2001-01-30 The Universtiy Of North Carolina At Chapel Hill Electropolymerizable film, and method of making and use thereof
US5854033A (en) * 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
DK0862656T3 (da) * 1995-11-21 2001-04-09 Univ Yale Unimolekylær segmentamplifikation og -detektering
US6458530B1 (en) * 1996-04-04 2002-10-01 Affymetrix Inc. Selecting tag nucleic acids
US5981297A (en) * 1997-02-05 1999-11-09 The United States Of America As Represented By The Secretary Of The Navy Biosensor using magnetically-detected label
US6221586B1 (en) * 1997-04-09 2001-04-24 California Institute Of Technology Electrochemical sensor using intercalative, redox-active moieties
US6124120A (en) * 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
US6686150B1 (en) * 1998-01-27 2004-02-03 Clinical Micro Sensors, Inc. Amplification of nucleic acids with electronic detection
US6316229B1 (en) * 1998-07-20 2001-11-13 Yale University Single molecule analysis target-mediated ligation of bipartite primers
US6232067B1 (en) * 1998-08-17 2001-05-15 The Perkin-Elmer Corporation Adapter directed expression analysis
US6287824B1 (en) * 1998-09-15 2001-09-11 Yale University Molecular cloning using rolling circle amplification
US6177248B1 (en) * 1999-02-24 2001-01-23 Affymetrix, Inc. Downstream genes of tumor suppressor WT1
JP2002539849A (ja) * 1999-03-26 2002-11-26 ホワイトヘッド インスチチュート フォアー バイオメディカル リサーチ ユニバーサルアレイ
EP1190092A2 (de) * 1999-04-06 2002-03-27 Yale University Analyse von sequenzmarkern mit vorgegebenen adressaten
US20010031468A1 (en) * 2000-02-08 2001-10-18 Alex Chenchik Analyte assays employing universal arrays
EP1967595A3 (de) * 2000-02-16 2008-12-03 Illumina, Inc. Paralleles Genotypisieren von mehreren Patientenproben
US20030119004A1 (en) * 2001-12-05 2003-06-26 Wenz H. Michael Methods for quantitating nucleic acids using coupled ligation and amplification

Also Published As

Publication number Publication date
EP1558764B1 (de) 2011-01-05
JP2006507014A (ja) 2006-03-02
EP1558764A2 (de) 2005-08-03
AU2003287533A1 (en) 2004-06-03
JP4505414B2 (ja) 2010-07-21
ATE494389T1 (de) 2011-01-15
CA2505254C (en) 2012-10-30
WO2004044549A3 (en) 2004-10-21
EP1558764A4 (de) 2006-05-24
CA2505254A1 (en) 2004-05-27
WO2004044549A2 (en) 2004-05-27
US20040086892A1 (en) 2004-05-06

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