DE60334466D1 - Entkopplung von dna-propagierung und proteinexpression für phagen-display - Google Patents
Entkopplung von dna-propagierung und proteinexpression für phagen-displayInfo
- Publication number
- DE60334466D1 DE60334466D1 DE60334466T DE60334466T DE60334466D1 DE 60334466 D1 DE60334466 D1 DE 60334466D1 DE 60334466 T DE60334466 T DE 60334466T DE 60334466 T DE60334466 T DE 60334466T DE 60334466 D1 DE60334466 D1 DE 60334466D1
- Authority
- DE
- Germany
- Prior art keywords
- display
- protein expression
- phage
- phag
- decoupling
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 229920001184 polypeptide Polymers 0.000 abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 3
- 101710132601 Capsid protein Proteins 0.000 abstract 2
- 101710094648 Coat protein Proteins 0.000 abstract 2
- 102100021181 Golgi phosphoprotein 3 Human genes 0.000 abstract 2
- 101710125418 Major capsid protein Proteins 0.000 abstract 2
- 101710141454 Nucleoprotein Proteins 0.000 abstract 2
- 101710083689 Probable capsid protein Proteins 0.000 abstract 2
- 230000004927 fusion Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 238000002823 phage display Methods 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Virology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/214,654 US7112435B1 (en) | 2002-08-07 | 2002-08-07 | Uncoupling of DNA insert propagation and expression of protein for phage display |
| PCT/US2003/024221 WO2004015142A1 (en) | 2002-08-07 | 2003-08-01 | Uncoupling of dna propagation and expression of protein for phage display |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE60334466D1 true DE60334466D1 (de) | 2010-11-18 |
Family
ID=31714248
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60334466T Expired - Lifetime DE60334466D1 (de) | 2002-08-07 | 2003-08-01 | Entkopplung von dna-propagierung und proteinexpression für phagen-display |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US7112435B1 (enExample) |
| EP (1) | EP1543160B1 (enExample) |
| JP (2) | JP4870351B2 (enExample) |
| AT (1) | ATE483810T1 (enExample) |
| AU (1) | AU2003257132B2 (enExample) |
| CA (1) | CA2493716A1 (enExample) |
| DE (1) | DE60334466D1 (enExample) |
| DK (1) | DK1543160T3 (enExample) |
| ES (1) | ES2350575T3 (enExample) |
| WO (1) | WO2004015142A1 (enExample) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7897381B2 (en) | 2002-08-07 | 2011-03-01 | Ambit Biosciences Corporation | Uncoupling of DNA insert propagation and expression of protein for phage display |
| US7833741B2 (en) * | 2002-08-07 | 2010-11-16 | Ambit Biosciences Corporation | Uncoupling of DNA insert propagation and expression of protein for phage display |
| CA2576195A1 (en) * | 2004-08-05 | 2006-02-16 | Biosite Incorporated | Compositions and methods for phage display of polypeptides |
| GB2428293A (en) * | 2005-07-13 | 2007-01-24 | Domantis Ltd | Phage display libraries |
| MY159327A (en) | 2009-02-27 | 2016-12-25 | Ambit Biosciences Corp | Jak kinase modulating quinazoline derivatives and methods of use thereof |
| MX2012001974A (es) | 2009-08-19 | 2012-04-11 | Ambit Biosciences Corp | Compuestos de biarilo y metodos de uso de los mismos. |
| WO2011056764A1 (en) | 2009-11-05 | 2011-05-12 | Ambit Biosciences Corp. | Isotopically enriched or fluorinated imidazo[2,1-b][1,3]benzothiazoles |
| US9296722B2 (en) | 2010-05-27 | 2016-03-29 | Ambit Biosciences Corporation | Azolyl urea compounds and methods of use thereof |
| WO2011150201A2 (en) | 2010-05-27 | 2011-12-01 | Ambit Biosciences Corporation | Azolyl amide compounds and methods of use thereof |
| EP2611793A1 (en) | 2010-09-01 | 2013-07-10 | Ambit Biosciences Corporation | 2-cycloquinazoline derivatives and methods of use thereof |
| EP2663553B1 (en) | 2010-09-01 | 2015-08-26 | Ambit Biosciences Corporation | Quinoline and isoquinoline derivatives for use as jak modulators |
| WO2012030948A1 (en) | 2010-09-01 | 2012-03-08 | Ambit Biosciences Corporation | Quinazoline compounds and methods of use thereof |
| EP2611448A1 (en) | 2010-09-01 | 2013-07-10 | Ambit Biosciences Corporation | 7-cyclylquinazoline derivatives and methods of use thereof |
| AU2011296078B2 (en) | 2010-09-01 | 2015-06-18 | Ambit Biosciences Corporation | An optically active pyrazolylaminoquinazoline, and pharmaceutical compositions and methods of use thereof |
| EP2611812A1 (en) | 2010-09-01 | 2013-07-10 | Ambit Biosciences Corporation | Thienopyridine and thienopyrimidine compounds and methods of use thereof |
| WO2012030924A1 (en) | 2010-09-01 | 2012-03-08 | Ambit Biosciences Corporation | Azolopyridine and azolopyrimidine compounds and methods of use thereof |
| US20130225614A1 (en) | 2010-09-01 | 2013-08-29 | Ambit Biosciences Corporation | 4-azolylaminoquinazoline derivatives and methods of use thereof |
| AU2012322095B2 (en) | 2011-10-14 | 2017-06-29 | Ambit Biosciences Corporation | Heterocyclic compounds and use thereof as modulators of type III receptor tyrosine kinases |
| US10829484B2 (en) | 2015-07-28 | 2020-11-10 | Plexxikon Inc. | Compounds and methods for kinase modulation, and indications therefor |
| WO2024020328A1 (en) | 2022-07-16 | 2024-01-25 | Eurofins Discoverx Corporation | Binding assays using phage display technology and kits thereof |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5693489A (en) | 1984-03-30 | 1997-12-02 | Associated Universities, Inc. | Cloning and expression of the gene for bacteriophage T7 RNA polymerase |
| US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| US5498538A (en) | 1990-02-15 | 1996-03-12 | The University Of North Carolina At Chapel Hill | Totally synthetic affinity reagents |
| US6172197B1 (en) | 1991-07-10 | 2001-01-09 | Medical Research Council | Methods for producing members of specific binding pairs |
| PT1024191E (pt) | 1991-12-02 | 2008-12-22 | Medical Res Council | Produção de auto-anticorpos a partir de reportórios de segmentos de anticorpo e exibidos em fagos |
| US5770356A (en) * | 1992-09-04 | 1998-06-23 | The Scripps Research Institute | Phagemids coexpressing a surface receptor and a surface heterologous protein |
| ES2156149T3 (es) | 1992-12-04 | 2001-06-16 | Medical Res Council | Proteinas de union multivalente y multiespecificas, su fabricacion y su uso. |
| US5766905A (en) | 1996-06-14 | 1998-06-16 | Associated Universities Inc. | Cytoplasmic bacteriophage display system |
| US20030124555A1 (en) * | 2001-05-21 | 2003-07-03 | Invitrogen Corporation | Compositions and methods for use in isolation of nucleic acid molecules |
| US6541011B2 (en) | 1998-02-11 | 2003-04-01 | Maxygen, Inc. | Antigen library immunization |
| JP2002522029A (ja) * | 1998-07-27 | 2002-07-23 | ジェネンテック・インコーポレーテッド | コートタンパク質の改変によるファージ提示における改良した形質転換効率 |
| CA2383186A1 (en) | 1999-09-03 | 2001-03-15 | Yale University | Method to identify gene function using small molecule probes |
| US7060506B2 (en) | 2000-01-31 | 2006-06-13 | Cyclacel, Ltd. | Compositions and methods for monitoring the modification of modification dependent binding partner polypeptides |
| WO2002085923A2 (en) | 2001-04-19 | 2002-10-31 | The Scripps Research Institute | In vivo incorporation of unnatural amino acids |
| US20030186221A1 (en) | 2002-04-02 | 2003-10-02 | Lockhart David J. | Phage display affinity filter and forward screen |
-
2002
- 2002-08-07 US US10/214,654 patent/US7112435B1/en not_active Expired - Lifetime
-
2003
- 2003-08-01 DE DE60334466T patent/DE60334466D1/de not_active Expired - Lifetime
- 2003-08-01 CA CA002493716A patent/CA2493716A1/en not_active Abandoned
- 2003-08-01 ES ES03784882T patent/ES2350575T3/es not_active Expired - Lifetime
- 2003-08-01 EP EP03784882A patent/EP1543160B1/en not_active Expired - Lifetime
- 2003-08-01 JP JP2004527717A patent/JP4870351B2/ja not_active Expired - Lifetime
- 2003-08-01 AU AU2003257132A patent/AU2003257132B2/en not_active Expired
- 2003-08-01 DK DK03784882.7T patent/DK1543160T3/da active
- 2003-08-01 WO PCT/US2003/024221 patent/WO2004015142A1/en not_active Ceased
- 2003-08-01 AT AT03784882T patent/ATE483810T1/de not_active IP Right Cessation
-
2011
- 2011-09-27 JP JP2011210341A patent/JP5389882B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JP2005534333A (ja) | 2005-11-17 |
| CA2493716A1 (en) | 2004-02-19 |
| EP1543160A4 (en) | 2007-03-21 |
| JP4870351B2 (ja) | 2012-02-08 |
| JP2012050444A (ja) | 2012-03-15 |
| EP1543160A1 (en) | 2005-06-22 |
| JP5389882B2 (ja) | 2014-01-15 |
| WO2004015142A1 (en) | 2004-02-19 |
| DK1543160T3 (da) | 2011-01-17 |
| AU2003257132A1 (en) | 2004-02-25 |
| ES2350575T3 (es) | 2011-01-25 |
| AU2003257132B2 (en) | 2011-12-15 |
| US7112435B1 (en) | 2006-09-26 |
| EP1543160B1 (en) | 2010-10-06 |
| ATE483810T1 (de) | 2010-10-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R082 | Change of representative |
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| R081 | Change of applicant/patentee |
Ref document number: 1543160 Country of ref document: EP Owner name: DISCOVERX CORP., US Free format text: FORMER OWNER: AMBIT BIOSCIENCES CORP., SAN DIEGO, US Effective date: 20120529 |
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