CA2493716A1 - Uncoupling of dna propagation and expression of protein for phage display - Google Patents

Uncoupling of dna propagation and expression of protein for phage display Download PDF

Info

Publication number
CA2493716A1
CA2493716A1 CA002493716A CA2493716A CA2493716A1 CA 2493716 A1 CA2493716 A1 CA 2493716A1 CA 002493716 A CA002493716 A CA 002493716A CA 2493716 A CA2493716 A CA 2493716A CA 2493716 A1 CA2493716 A1 CA 2493716A1
Authority
CA
Canada
Prior art keywords
phage
polypeptide
test compound
sequence encoding
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002493716A
Other languages
English (en)
French (fr)
Inventor
Pietro Ciceri
Patrick Parvis Zarrinkar
Daniel Kelly Treiber
David J. Lockhart
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DiscoveRx Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2493716A1 publication Critical patent/CA2493716A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CA002493716A 2002-08-07 2003-08-01 Uncoupling of dna propagation and expression of protein for phage display Abandoned CA2493716A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10/214,654 2002-08-07
US10/214,654 US7112435B1 (en) 2002-08-07 2002-08-07 Uncoupling of DNA insert propagation and expression of protein for phage display
PCT/US2003/024221 WO2004015142A1 (en) 2002-08-07 2003-08-01 Uncoupling of dna propagation and expression of protein for phage display

Publications (1)

Publication Number Publication Date
CA2493716A1 true CA2493716A1 (en) 2004-02-19

Family

ID=31714248

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002493716A Abandoned CA2493716A1 (en) 2002-08-07 2003-08-01 Uncoupling of dna propagation and expression of protein for phage display

Country Status (10)

Country Link
US (1) US7112435B1 (enExample)
EP (1) EP1543160B1 (enExample)
JP (2) JP4870351B2 (enExample)
AT (1) ATE483810T1 (enExample)
AU (1) AU2003257132B2 (enExample)
CA (1) CA2493716A1 (enExample)
DE (1) DE60334466D1 (enExample)
DK (1) DK1543160T3 (enExample)
ES (1) ES2350575T3 (enExample)
WO (1) WO2004015142A1 (enExample)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7897381B2 (en) 2002-08-07 2011-03-01 Ambit Biosciences Corporation Uncoupling of DNA insert propagation and expression of protein for phage display
US7833741B2 (en) * 2002-08-07 2010-11-16 Ambit Biosciences Corporation Uncoupling of DNA insert propagation and expression of protein for phage display
CA2576195A1 (en) * 2004-08-05 2006-02-16 Biosite Incorporated Compositions and methods for phage display of polypeptides
GB2428293A (en) * 2005-07-13 2007-01-24 Domantis Ltd Phage display libraries
MY159327A (en) 2009-02-27 2016-12-25 Ambit Biosciences Corp Jak kinase modulating quinazoline derivatives and methods of use thereof
MX2012001974A (es) 2009-08-19 2012-04-11 Ambit Biosciences Corp Compuestos de biarilo y metodos de uso de los mismos.
WO2011056764A1 (en) 2009-11-05 2011-05-12 Ambit Biosciences Corp. Isotopically enriched or fluorinated imidazo[2,1-b][1,3]benzothiazoles
US9296722B2 (en) 2010-05-27 2016-03-29 Ambit Biosciences Corporation Azolyl urea compounds and methods of use thereof
WO2011150201A2 (en) 2010-05-27 2011-12-01 Ambit Biosciences Corporation Azolyl amide compounds and methods of use thereof
EP2611793A1 (en) 2010-09-01 2013-07-10 Ambit Biosciences Corporation 2-cycloquinazoline derivatives and methods of use thereof
EP2663553B1 (en) 2010-09-01 2015-08-26 Ambit Biosciences Corporation Quinoline and isoquinoline derivatives for use as jak modulators
WO2012030948A1 (en) 2010-09-01 2012-03-08 Ambit Biosciences Corporation Quinazoline compounds and methods of use thereof
EP2611448A1 (en) 2010-09-01 2013-07-10 Ambit Biosciences Corporation 7-cyclylquinazoline derivatives and methods of use thereof
AU2011296078B2 (en) 2010-09-01 2015-06-18 Ambit Biosciences Corporation An optically active pyrazolylaminoquinazoline, and pharmaceutical compositions and methods of use thereof
EP2611812A1 (en) 2010-09-01 2013-07-10 Ambit Biosciences Corporation Thienopyridine and thienopyrimidine compounds and methods of use thereof
WO2012030924A1 (en) 2010-09-01 2012-03-08 Ambit Biosciences Corporation Azolopyridine and azolopyrimidine compounds and methods of use thereof
US20130225614A1 (en) 2010-09-01 2013-08-29 Ambit Biosciences Corporation 4-azolylaminoquinazoline derivatives and methods of use thereof
AU2012322095B2 (en) 2011-10-14 2017-06-29 Ambit Biosciences Corporation Heterocyclic compounds and use thereof as modulators of type III receptor tyrosine kinases
US10829484B2 (en) 2015-07-28 2020-11-10 Plexxikon Inc. Compounds and methods for kinase modulation, and indications therefor
WO2024020328A1 (en) 2022-07-16 2024-01-25 Eurofins Discoverx Corporation Binding assays using phage display technology and kits thereof

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5693489A (en) 1984-03-30 1997-12-02 Associated Universities, Inc. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
US5498538A (en) 1990-02-15 1996-03-12 The University Of North Carolina At Chapel Hill Totally synthetic affinity reagents
US6172197B1 (en) 1991-07-10 2001-01-09 Medical Research Council Methods for producing members of specific binding pairs
PT1024191E (pt) 1991-12-02 2008-12-22 Medical Res Council Produção de auto-anticorpos a partir de reportórios de segmentos de anticorpo e exibidos em fagos
US5770356A (en) * 1992-09-04 1998-06-23 The Scripps Research Institute Phagemids coexpressing a surface receptor and a surface heterologous protein
ES2156149T3 (es) 1992-12-04 2001-06-16 Medical Res Council Proteinas de union multivalente y multiespecificas, su fabricacion y su uso.
US5766905A (en) 1996-06-14 1998-06-16 Associated Universities Inc. Cytoplasmic bacteriophage display system
US20030124555A1 (en) * 2001-05-21 2003-07-03 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
US6541011B2 (en) 1998-02-11 2003-04-01 Maxygen, Inc. Antigen library immunization
JP2002522029A (ja) * 1998-07-27 2002-07-23 ジェネンテック・インコーポレーテッド コートタンパク質の改変によるファージ提示における改良した形質転換効率
CA2383186A1 (en) 1999-09-03 2001-03-15 Yale University Method to identify gene function using small molecule probes
US7060506B2 (en) 2000-01-31 2006-06-13 Cyclacel, Ltd. Compositions and methods for monitoring the modification of modification dependent binding partner polypeptides
WO2002085923A2 (en) 2001-04-19 2002-10-31 The Scripps Research Institute In vivo incorporation of unnatural amino acids
US20030186221A1 (en) 2002-04-02 2003-10-02 Lockhart David J. Phage display affinity filter and forward screen

Also Published As

Publication number Publication date
JP2005534333A (ja) 2005-11-17
EP1543160A4 (en) 2007-03-21
JP4870351B2 (ja) 2012-02-08
JP2012050444A (ja) 2012-03-15
EP1543160A1 (en) 2005-06-22
JP5389882B2 (ja) 2014-01-15
WO2004015142A1 (en) 2004-02-19
DK1543160T3 (da) 2011-01-17
DE60334466D1 (de) 2010-11-18
AU2003257132A1 (en) 2004-02-25
ES2350575T3 (es) 2011-01-25
AU2003257132B2 (en) 2011-12-15
US7112435B1 (en) 2006-09-26
EP1543160B1 (en) 2010-10-06
ATE483810T1 (de) 2010-10-15

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued