DE60319825D1 - Isolierung von nukleinsäuren unter verwendung eines polykationischen polymers als fällungsmittel - Google Patents

Isolierung von nukleinsäuren unter verwendung eines polykationischen polymers als fällungsmittel

Info

Publication number
DE60319825D1
DE60319825D1 DE60319825T DE60319825T DE60319825D1 DE 60319825 D1 DE60319825 D1 DE 60319825D1 DE 60319825 T DE60319825 T DE 60319825T DE 60319825 T DE60319825 T DE 60319825T DE 60319825 D1 DE60319825 D1 DE 60319825D1
Authority
DE
Germany
Prior art keywords
nucleic acid
precipitating agent
polycationic
polymer
polycanic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60319825T
Other languages
English (en)
Other versions
DE60319825T2 (de
Inventor
Igor Yu Galaev
Per-Erik Gustavsson
Vladimir Izumrudov
Per-Olof Larsson
Per-Olof Wahlund
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cytiva Sweden AB
Original Assignee
GE Healthcare Bio Sciences AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GE Healthcare Bio Sciences AB filed Critical GE Healthcare Bio Sciences AB
Publication of DE60319825D1 publication Critical patent/DE60319825D1/de
Application granted granted Critical
Publication of DE60319825T2 publication Critical patent/DE60319825T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DE60319825T 2002-06-28 2003-06-26 Isolierung von nukleinsäuren unter verwendung eines polykationischen polymers als fällungsmittel Expired - Fee Related DE60319825T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
SE0202074 2002-06-28
SE0202074A SE0202074D0 (sv) 2002-06-28 2002-06-28 Isolation of nucleic acids
SE0301034A SE0301034D0 (sv) 2002-06-28 2003-04-08 Improved isolation of nucleic acids
SE0301034 2003-04-08
PCT/SE2003/001127 WO2004003200A1 (en) 2002-06-28 2003-06-26 Isolation of nucleic acids using a polycationic polymer as precipitation agent

Publications (2)

Publication Number Publication Date
DE60319825D1 true DE60319825D1 (de) 2008-04-30
DE60319825T2 DE60319825T2 (de) 2009-03-05

Family

ID=26655712

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60319825T Expired - Fee Related DE60319825T2 (de) 2002-06-28 2003-06-26 Isolierung von nukleinsäuren unter verwendung eines polykationischen polymers als fällungsmittel

Country Status (10)

Country Link
US (1) US20050222404A1 (de)
EP (1) EP1517990B1 (de)
JP (1) JP2005531329A (de)
AT (1) ATE389718T1 (de)
AU (1) AU2003243108A1 (de)
CA (1) CA2488616A1 (de)
DE (1) DE60319825T2 (de)
ES (1) ES2301823T3 (de)
SE (2) SE0202074D0 (de)
WO (1) WO2004003200A1 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT501380B1 (de) * 2005-02-07 2007-11-15 Vitali Dipl Ing Dshemelinski Verfahren zur reinigung der dna/rna von proteinen und zellulären reststoffen im zellenlysat
DE102005015005A1 (de) * 2005-04-01 2006-10-05 Qiagen Gmbh Verfahren zur Behandlung einer Biomoleküle enthaltenden Probe
US20060234251A1 (en) * 2005-04-19 2006-10-19 Lumigen, Inc. Methods of enhancing isolation of RNA from biological samples
CN101849024B (zh) * 2007-11-05 2015-11-25 荣研化学株式会社 核酸扩增用样品的制备方法及制备试剂盒
US20130158247A1 (en) 2010-06-01 2013-06-20 Qiagen Gmbh Method for isolating and/or purifying nucleic acid(s)
US9604213B2 (en) 2012-02-13 2017-03-28 Neumodx Molecular, Inc. System and method for processing and detecting nucleic acids
CN114134029A (zh) 2012-02-13 2022-03-04 纽莫德克斯莫勒库拉尔公司 用于处理和检测核酸的微流体盒
US11485968B2 (en) 2012-02-13 2022-11-01 Neumodx Molecular, Inc. Microfluidic cartridge for processing and detecting nucleic acids
US9637775B2 (en) 2012-02-13 2017-05-02 Neumodx Molecular, Inc. System and method for processing biological samples
US11648561B2 (en) 2012-02-13 2023-05-16 Neumodx Molecular, Inc. System and method for processing and detecting nucleic acids
EP2912174B1 (de) 2012-10-25 2019-06-19 Neumodx Molecular, Inc. Verfahren und materialien zur isolierung von nukleinsäurematerialien
CN111094564A (zh) 2017-07-12 2020-05-01 伊鲁米纳公司 核酸提取材料、系统和方法
CN114133991A (zh) * 2021-08-27 2022-03-04 深圳市朗司医疗科技有限公司 一种核酸清洗组合试剂及使用方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4055469A (en) * 1976-12-10 1977-10-25 Eastman Kodak Company Purification of microbial enzyme extracts using synthetic polyelectrolytes
US5599667A (en) * 1987-03-02 1997-02-04 Gen-Probe Incorporated Polycationic supports and nucleic acid purification separation and hybridization
EP0281390B1 (de) * 1987-03-02 1994-06-22 Gen-Probe Incorporated Polykationische Träger zur Reinigung, Trennung und Hybridisierung von Nukleinsäure
US5010183A (en) * 1989-07-07 1991-04-23 Macfarlane Donald E Process for purifying DNA and RNA using cationic detergents
CA2299119C (en) * 1999-02-23 2013-02-05 Qiagen Gmbh A method of stabilizing and/or isolating nucleic acids
US20020010145A1 (en) * 1999-07-12 2002-01-24 Willson Richard C. Apparatus, methods and compositions for biotechnical separations

Also Published As

Publication number Publication date
ATE389718T1 (de) 2008-04-15
DE60319825T2 (de) 2009-03-05
EP1517990B1 (de) 2008-03-19
CA2488616A1 (en) 2004-01-08
EP1517990A1 (de) 2005-03-30
SE0301034D0 (sv) 2003-04-08
US20050222404A1 (en) 2005-10-06
WO2004003200A1 (en) 2004-01-08
JP2005531329A (ja) 2005-10-20
SE0202074D0 (sv) 2002-07-02
ES2301823T3 (es) 2008-07-01
AU2003243108A1 (en) 2004-01-19

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8328 Change in the person/name/address of the agent

Representative=s name: HAMMONDS LLP, LONDON, GB

8339 Ceased/non-payment of the annual fee