DE60311470T2 - Verfahren zur reinigung von interleukin-4 und seinen muteinen - Google Patents
Verfahren zur reinigung von interleukin-4 und seinen muteinen Download PDFInfo
- Publication number
- DE60311470T2 DE60311470T2 DE60311470T DE60311470T DE60311470T2 DE 60311470 T2 DE60311470 T2 DE 60311470T2 DE 60311470 T DE60311470 T DE 60311470T DE 60311470 T DE60311470 T DE 60311470T DE 60311470 T2 DE60311470 T2 DE 60311470T2
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- Prior art keywords
- interleukin
- buffer
- inclusion bodies
- refolding
- detergent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- MGFYIUFZLHCRTH-UHFFFAOYSA-N nitrilotriacetic acid Chemical compound OC(=O)CN(CC(O)=O)CC(O)=O MGFYIUFZLHCRTH-UHFFFAOYSA-N 0.000 description 1
- JCXJVPUVTGWSNB-UHFFFAOYSA-N nitrogen dioxide Inorganic materials O=[N]=O JCXJVPUVTGWSNB-UHFFFAOYSA-N 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- IFPHDUVGLXEIOQ-UHFFFAOYSA-N ortho-iodosylbenzoic acid Chemical compound OC(=O)C1=CC=CC=C1I=O IFPHDUVGLXEIOQ-UHFFFAOYSA-N 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 229920001451 polypropylene glycol Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 238000002731 protein assay Methods 0.000 description 1
- 238000001742 protein purification Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 239000012925 reference material Substances 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 235000011008 sodium phosphates Nutrition 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 229960000776 sodium tetradecyl sulfate Drugs 0.000 description 1
- UPUIQOIQVMNQAP-UHFFFAOYSA-M sodium;tetradecyl sulfate Chemical compound [Na+].CCCCCCCCCCCCCCOS([O-])(=O)=O UPUIQOIQVMNQAP-UHFFFAOYSA-M 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000011146 sterile filtration Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000007929 subcutaneous injection Substances 0.000 description 1
- 238000010254 subcutaneous injection Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- WBWWGRHZICKQGZ-GIHLXUJPSA-N taurocholic acid Chemical compound C([C@@H]1C[C@H]2O)[C@@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@@H]([C@@H](CCC(=O)NCCS(O)(=O)=O)C)[C@@]2(C)[C@H](O)C1 WBWWGRHZICKQGZ-GIHLXUJPSA-N 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- HHLJUSLZGFYWKW-UHFFFAOYSA-N triethanolamine hydrochloride Chemical compound Cl.OCCN(CCO)CCO HHLJUSLZGFYWKW-UHFFFAOYSA-N 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
- G01N33/6869—Interleukin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5406—IL-4
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/52—Assays involving cytokines
- G01N2333/54—Interleukins [IL]
- G01N2333/5406—IL-4
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Organic Chemistry (AREA)
- Hematology (AREA)
- General Health & Medical Sciences (AREA)
- Urology & Nephrology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Toxicology (AREA)
- Genetics & Genomics (AREA)
- Food Science & Technology (AREA)
- Microbiology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP02015589 | 2002-07-15 | ||
| EP02015589A EP1382614A1 (en) | 2002-07-15 | 2002-07-15 | Process for the purification of interleukin-4 and its muteins |
| PCT/EP2003/007022 WO2004007549A1 (en) | 2002-07-15 | 2003-07-02 | Process for the purification of interleukin-4 and its muteins |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60311470D1 DE60311470D1 (de) | 2007-03-15 |
| DE60311470T2 true DE60311470T2 (de) | 2007-10-31 |
Family
ID=29762614
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE60311470T Expired - Lifetime DE60311470T2 (de) | 2002-07-15 | 2003-07-02 | Verfahren zur reinigung von interleukin-4 und seinen muteinen |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US20060134690A1 (https=) |
| EP (2) | EP1382614A1 (https=) |
| JP (2) | JP2006506332A (https=) |
| AT (1) | ATE352566T1 (https=) |
| AU (1) | AU2003244635A1 (https=) |
| DE (1) | DE60311470T2 (https=) |
| ES (1) | ES2280764T3 (https=) |
| WO (1) | WO2004007549A1 (https=) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1382614A1 (en) * | 2002-07-15 | 2004-01-21 | Bayer HealthCare AG | Process for the purification of interleukin-4 and its muteins |
| US8189621B2 (en) | 2006-05-12 | 2012-05-29 | Microsoft Corporation | Stack signaling to application with lack of requested bandwidth |
| US8144793B2 (en) | 2006-12-12 | 2012-03-27 | Microsoft Corporation | Cognitive multi-user OFDMA |
| US8241697B2 (en) | 2007-12-20 | 2012-08-14 | Abbott Point Of Care Inc. | Formation of immobilized biological layers for sensing |
| US8268604B2 (en) * | 2007-12-20 | 2012-09-18 | Abbott Point Of Care Inc. | Compositions for forming immobilized biological layers for sensing |
| KR101113495B1 (ko) | 2008-01-23 | 2012-02-29 | 한미홀딩스 주식회사 | 인간 인터페론 베타의 정제방법 |
| WO2010105227A1 (en) * | 2009-03-13 | 2010-09-16 | Aerovance, Inc. | Methods of renaturation of recombinant proteins |
| WO2010128141A1 (en) * | 2009-05-07 | 2010-11-11 | Novo Nordisk Health Care Ag | Stable pharmaceutical compositions of peptide derivatized using an oxime linker |
| EP2611419A2 (en) * | 2010-08-30 | 2013-07-10 | F.Hoffmann-La Roche Ag | Method for producing a tetranectin-apolipoprotein a-1 lipid particle, the lipid particle itself and its use |
| CN103068369A (zh) * | 2010-08-30 | 2013-04-24 | 霍夫曼-拉罗奇有限公司 | 产生四连蛋白-载脂蛋白a-i颗粒的方法，由此获得的脂质颗粒及其应用 |
| CN103320447A (zh) * | 2013-05-20 | 2013-09-25 | 深圳市亚太兴实业有限公司 | 重组人白介素4基因、含重组人白介素4基因的工程菌发酵方法 |
| CN108753753B (zh) * | 2018-05-28 | 2022-04-22 | 中国林业科学研究院林业研究所 | Lrr受体激酶-pxy的纯化方法 |
| CN114599665A (zh) | 2019-08-16 | 2022-06-07 | 应用分子转运公司 | 组合物、制剂及白细胞介素产生和纯化 |
| CN116874552B (zh) * | 2023-09-08 | 2023-12-08 | 成都华任康生物科技有限公司 | 一种目标蛋白的纯化方法及其试剂盒和相关应用 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4137333A1 (de) * | 1991-11-13 | 1993-05-19 | W Prof Dr Sebald | Therapeutische mittel, die antagonisten oder partielle agonisten des humanen interleukin 4 sind oder diese enthalten, hil-4-mutantenproteine sowie vefahren zu deren herstellung |
| CA2155335C (en) * | 1993-02-04 | 2001-06-05 | HANS CHRISTIAN THõGERSEN | Improved method for the refolding of proteins |
| US5563057A (en) * | 1994-10-31 | 1996-10-08 | Wisconsin Alumni Research Foundation | Method for refolding misfolded enzymes with detergent and cyclodextrin |
| JP3344618B2 (ja) * | 1997-06-20 | 2002-11-11 | 株式会社エイチ・エス・ピー研究所 | シャペロン発現プラスミド |
| US5932102A (en) * | 1998-01-12 | 1999-08-03 | Schering Corporation | Immobilized metal, affinity chromatography |
| EP1382614A1 (en) * | 2002-07-15 | 2004-01-21 | Bayer HealthCare AG | Process for the purification of interleukin-4 and its muteins |
-
2002
- 2002-07-15 EP EP02015589A patent/EP1382614A1/en not_active Withdrawn
-
2003
- 2003-07-02 DE DE60311470T patent/DE60311470T2/de not_active Expired - Lifetime
- 2003-07-02 AT AT03738109T patent/ATE352566T1/de not_active IP Right Cessation
- 2003-07-02 US US10/520,883 patent/US20060134690A1/en not_active Abandoned
- 2003-07-02 WO PCT/EP2003/007022 patent/WO2004007549A1/en not_active Ceased
- 2003-07-02 EP EP03738109A patent/EP1523500B1/en not_active Expired - Lifetime
- 2003-07-02 JP JP2004520457A patent/JP2006506332A/ja active Pending
- 2003-07-02 AU AU2003244635A patent/AU2003244635A1/en not_active Abandoned
- 2003-07-02 ES ES03738109T patent/ES2280764T3/es not_active Expired - Lifetime
-
2010
- 2010-11-12 US US12/945,829 patent/US20110060128A1/en not_active Abandoned
- 2010-12-24 JP JP2010288204A patent/JP2011088922A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2011088922A (ja) | 2011-05-06 |
| EP1523500B1 (en) | 2007-01-24 |
| EP1523500A1 (en) | 2005-04-20 |
| AU2003244635A1 (en) | 2004-02-02 |
| ATE352566T1 (de) | 2007-02-15 |
| US20060134690A1 (en) | 2006-06-22 |
| EP1382614A1 (en) | 2004-01-21 |
| DE60311470D1 (de) | 2007-03-15 |
| US20110060128A1 (en) | 2011-03-10 |
| ES2280764T3 (es) | 2007-09-16 |
| WO2004007549A1 (en) | 2004-01-22 |
| JP2006506332A (ja) | 2006-02-23 |
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| 8364 | No opposition during term of opposition | ||
| 8327 | Change in the person/name/address of the patent owner |
Owner name: BAYER SCHERING PHARMA AKTIENGESELLSCHAFT, 1335, DE |