DE602005006941D1 - Verfahren zum Kontaminationsschutz von DNA-Amplifikationsreaktionen zur Methylierungsanalyse durch modifizierte Vorbehandlung der Nukleinsäuren - Google Patents
Verfahren zum Kontaminationsschutz von DNA-Amplifikationsreaktionen zur Methylierungsanalyse durch modifizierte Vorbehandlung der NukleinsäurenInfo
- Publication number
- DE602005006941D1 DE602005006941D1 DE602005006941T DE602005006941T DE602005006941D1 DE 602005006941 D1 DE602005006941 D1 DE 602005006941D1 DE 602005006941 T DE602005006941 T DE 602005006941T DE 602005006941 T DE602005006941 T DE 602005006941T DE 602005006941 D1 DE602005006941 D1 DE 602005006941D1
- Authority
- DE
- Germany
- Prior art keywords
- dna
- ung
- methylation analysis
- nucleic acids
- dna amplification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title abstract 4
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 102000039446 nucleic acids Human genes 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 title abstract 3
- 238000004458 analytical method Methods 0.000 title abstract 2
- 230000004544 DNA amplification Effects 0.000 title 1
- 238000006243 chemical reaction Methods 0.000 title 1
- 238000011109 contamination Methods 0.000 title 1
- 230000011987 methylation Effects 0.000 title 1
- 238000007069 methylation reaction Methods 0.000 title 1
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical class O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 abstract 4
- 230000003321 amplification Effects 0.000 abstract 3
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 3
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 abstract 2
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 abstract 2
- 230000007067 DNA methylation Effects 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 229940104302 cytosine Drugs 0.000 abstract 1
- 230000009615 deamination Effects 0.000 abstract 1
- 238000006481 deamination reaction Methods 0.000 abstract 1
- 238000005202 decontamination Methods 0.000 abstract 1
- 230000003588 decontaminative effect Effects 0.000 abstract 1
- 230000006326 desulfonation Effects 0.000 abstract 1
- 238000005869 desulfonation reaction Methods 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 abstract 1
- 230000002779 inactivation Effects 0.000 abstract 1
- 238000006277 sulfonation reaction Methods 0.000 abstract 1
- 229940035893 uracil Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP04090389 | 2004-10-11 | ||
| US61826704P | 2004-10-12 | 2004-10-12 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE602005006941D1 true DE602005006941D1 (de) | 2008-07-03 |
Family
ID=36000858
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE602005006941T Active DE602005006941D1 (de) | 2004-10-11 | 2005-10-11 | Verfahren zum Kontaminationsschutz von DNA-Amplifikationsreaktionen zur Methylierungsanalyse durch modifizierte Vorbehandlung der Nukleinsäuren |
Country Status (11)
| Country | Link |
|---|---|
| US (4) | US20080096199A1 (de) |
| EP (1) | EP1659186B1 (de) |
| JP (1) | JP4727670B2 (de) |
| AT (1) | ATE396282T1 (de) |
| AU (1) | AU2005293703B2 (de) |
| CA (1) | CA2581500C (de) |
| DE (1) | DE602005006941D1 (de) |
| DK (1) | DK1659186T3 (de) |
| ES (1) | ES2308376T3 (de) |
| PT (1) | PT1659186E (de) |
| WO (1) | WO2006040187A2 (de) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE60322044D1 (de) | 2002-02-21 | 2008-08-21 | Asm Scient Inc | Rekombinase-polymerase-amplifikation |
| US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
| US7399590B2 (en) | 2002-02-21 | 2008-07-15 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| US7435561B2 (en) | 2005-07-25 | 2008-10-14 | Asm Scientific, Inc. | Methods for multiplexing recombinase polymerase amplification |
| EP2029782B1 (de) | 2006-05-04 | 2014-11-26 | Alere San Diego, Inc. | Rekombinase-polymerase-verstärkung |
| CN101153336B (zh) | 2006-09-27 | 2011-09-07 | 香港中文大学 | 检测dna甲基化程度的方法和试剂盒 |
| JP2008136404A (ja) * | 2006-11-30 | 2008-06-19 | Sysmex Corp | Ｄｎａメチル化検出における非メチル化シトシン変換処理後のｄｎａ量の確認方法 |
| DK2479289T3 (en) | 2007-06-08 | 2016-05-02 | Epigenomics Ag | Method for methylation analysis |
| EP2201131B8 (de) * | 2007-09-17 | 2014-12-10 | MDxHealth SA | Verbesserter methylierungsnachweis |
| EP2071035A1 (de) | 2007-12-13 | 2009-06-17 | Epigenomics AG | Vermittler und Verstärkungsverfahren |
| EP2189538A1 (de) | 2008-11-21 | 2010-05-26 | Epigenomics AG | Analysemolekül und Verfahren zur Analyse einer Nucleotid-Position in einer Nukleinsäure |
| FR2940805B1 (fr) | 2009-01-05 | 2015-10-16 | Biomerieux Sa | Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede |
| US8039215B2 (en) * | 2009-03-10 | 2011-10-18 | Roche Molecular Systems, Inc. | Multiplex quantitative nucleic acid amplification and melting assay |
| WO2010135310A1 (en) | 2009-05-20 | 2010-11-25 | Biosite Incorporated | Dna glycosylase/lyase and ap endonuclease substrates |
| WO2010141940A1 (en) | 2009-06-05 | 2010-12-09 | Alere San Diego, Inc. | Recombinase polymerase amplification reagents and kits |
| US8846673B2 (en) | 2009-08-11 | 2014-09-30 | Bristol-Myers Squibb Company | Azaindazoles as kinase inhibitors and use thereof |
| US9777319B2 (en) | 2012-06-29 | 2017-10-03 | General Electric Company | Method for isothermal DNA amplification starting from an RNA template |
| JP6455438B2 (ja) * | 2012-12-13 | 2019-01-23 | ワン バイオームド ピーティーイー リミテッド | 固相デバイスにおける核酸の単離及び分析のためのラベルフリー方法 |
| JP6623324B2 (ja) * | 2015-09-07 | 2019-12-25 | 株式会社ファスマック | 等温増幅反応産物の多項目同時検出方法 |
| CN116445593A (zh) | 2016-08-10 | 2023-07-18 | 格里尔公司 | 测定一生物样品的一甲基化图谱的方法 |
| DE202019005627U1 (de) | 2018-04-02 | 2021-05-31 | Grail, Inc. | Methylierungsmarker und gezielte Methylierungssondenpanels |
| CN113286881A (zh) | 2018-09-27 | 2021-08-20 | 格里尔公司 | 甲基化标记和标靶甲基化探针板 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2122203C (en) * | 1993-05-11 | 2001-12-18 | Melinda S. Fraiser | Decontamination of nucleic acid amplification reactions |
| US6369237B1 (en) * | 1997-03-07 | 2002-04-09 | President And Fellows Of Harvard College | DNA glycosylase inhibitors, and uses related thereto |
| WO2002086169A1 (en) * | 2001-04-23 | 2002-10-31 | Dana-Farber Cancer Institute, Inc. | Methods for rapid screening of polymorphisms, mutations and methylation |
| US6960436B2 (en) * | 2002-02-06 | 2005-11-01 | Epigenomics Ag | Quantitative methylation detection in DNA samples |
| AUPS064202A0 (en) | 2002-02-20 | 2002-03-14 | Biomolecular Research Solutions Pty Ltd | Improved method for the analysis of nucleic acid samples |
| WO2006034009A2 (en) * | 2004-09-20 | 2006-03-30 | Sigma-Aldrich Co. | Purification of biomolecules from contaminating intact nucleic acids |
-
2005
- 2005-10-11 DK DK05090284T patent/DK1659186T3/da active
- 2005-10-11 AU AU2005293703A patent/AU2005293703B2/en not_active Ceased
- 2005-10-11 AT AT05090284T patent/ATE396282T1/de active
- 2005-10-11 DE DE602005006941T patent/DE602005006941D1/de active Active
- 2005-10-11 WO PCT/EP2005/011133 patent/WO2006040187A2/en active Application Filing
- 2005-10-11 US US11/665,196 patent/US20080096199A1/en not_active Abandoned
- 2005-10-11 CA CA2581500A patent/CA2581500C/en active Active
- 2005-10-11 ES ES05090284T patent/ES2308376T3/es active Active
- 2005-10-11 US US11/248,721 patent/US7700282B2/en active Active
- 2005-10-11 JP JP2007536113A patent/JP4727670B2/ja active Active
- 2005-10-11 EP EP05090284A patent/EP1659186B1/de active Active
- 2005-10-11 PT PT05090284T patent/PT1659186E/pt unknown
-
2010
- 2010-02-19 US US12/709,300 patent/US8753810B2/en active Active
-
2014
- 2014-06-13 US US14/304,836 patent/US20140295445A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1659186B1 (de) | 2008-05-21 |
| EP1659186A1 (de) | 2006-05-24 |
| DK1659186T3 (da) | 2008-09-22 |
| PT1659186E (pt) | 2008-08-29 |
| US8753810B2 (en) | 2014-06-17 |
| US20080096199A1 (en) | 2008-04-24 |
| AU2005293703B2 (en) | 2010-02-18 |
| WO2006040187A3 (en) | 2006-10-05 |
| US20060115835A1 (en) | 2006-06-01 |
| US7700282B2 (en) | 2010-04-20 |
| ES2308376T3 (es) | 2008-12-01 |
| ATE396282T1 (de) | 2008-06-15 |
| US20140295445A1 (en) | 2014-10-02 |
| CA2581500C (en) | 2013-02-12 |
| CA2581500A1 (en) | 2006-04-20 |
| AU2005293703A1 (en) | 2006-04-20 |
| US20100216152A1 (en) | 2010-08-26 |
| WO2006040187A2 (en) | 2006-04-20 |
| JP2008515447A (ja) | 2008-05-15 |
| JP4727670B2 (ja) | 2011-07-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8364 | No opposition during term of opposition | ||
| R082 | Change of representative |
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