DE60142384D1 - Verfahren zur herstellung von aktive, heterodimere, amv-reverse transkriptase in prokaryonten - Google Patents

Verfahren zur herstellung von aktive, heterodimere, amv-reverse transkriptase in prokaryonten

Info

Publication number
DE60142384D1
DE60142384D1 DE60142384T DE60142384T DE60142384D1 DE 60142384 D1 DE60142384 D1 DE 60142384D1 DE 60142384 T DE60142384 T DE 60142384T DE 60142384 T DE60142384 T DE 60142384T DE 60142384 D1 DE60142384 D1 DE 60142384D1
Authority
DE
Germany
Prior art keywords
amv
reverse transcriptase
prokaryonts
heterodimer
active
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60142384T
Other languages
English (en)
Inventor
Harald Dr Sobek
Rainer Dr Mueller
Manfred Schmidt
Bruno Dr Frey
Bernhard Dr Suppmann
Rainer Dr Schmuck
Johann-Peter Dr Thalhofer
Peter Dr Pallua
Markus Dr Pajatsch
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Priority to DE60142384T priority Critical patent/DE60142384D1/de
Application granted granted Critical
Publication of DE60142384D1 publication Critical patent/DE60142384D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1276—RNA-directed DNA polymerase (2.7.7.49), i.e. reverse transcriptase or telomerase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DE60142384T 2000-09-22 2001-09-19 Verfahren zur herstellung von aktive, heterodimere, amv-reverse transkriptase in prokaryonten Expired - Lifetime DE60142384D1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE60142384T DE60142384D1 (de) 2000-09-22 2001-09-19 Verfahren zur herstellung von aktive, heterodimere, amv-reverse transkriptase in prokaryonten

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10046960A DE10046960A1 (de) 2000-09-22 2000-09-22 Verfahren zur Herstellung einer aktiven, heterodimeren AMW-RT in prokaryotischen Zellen
DE60142384T DE60142384D1 (de) 2000-09-22 2001-09-19 Verfahren zur herstellung von aktive, heterodimere, amv-reverse transkriptase in prokaryonten

Publications (1)

Publication Number Publication Date
DE60142384D1 true DE60142384D1 (de) 2010-07-29

Family

ID=7657206

Family Applications (2)

Application Number Title Priority Date Filing Date
DE10046960A Ceased DE10046960A1 (de) 2000-09-22 2000-09-22 Verfahren zur Herstellung einer aktiven, heterodimeren AMW-RT in prokaryotischen Zellen
DE60142384T Expired - Lifetime DE60142384D1 (de) 2000-09-22 2001-09-19 Verfahren zur herstellung von aktive, heterodimere, amv-reverse transkriptase in prokaryonten

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DE10046960A Ceased DE10046960A1 (de) 2000-09-22 2000-09-22 Verfahren zur Herstellung einer aktiven, heterodimeren AMW-RT in prokaryotischen Zellen

Country Status (8)

Country Link
US (1) US6902920B2 (de)
EP (1) EP1191102B1 (de)
JP (1) JP2002315584A (de)
CN (1) CN1250728C (de)
AT (1) ATE471376T1 (de)
CA (1) CA2357540C (de)
DE (2) DE10046960A1 (de)
ES (1) ES2346511T3 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030219729A1 (en) * 2002-03-15 2003-11-27 Tosoh Corporation Unary avian myeloblastosis virus revers transcriptase and its use
EP2059258B1 (de) * 2006-09-08 2019-11-13 Wyeth LLC Argininwaschung bei der proteinreinigung mittels affinitätschromatografie
ME02505B (me) * 2009-12-29 2017-02-20 Aptevo Res & Development Llc Heterodimerni vezujući proteini i njihove upotrebe
JP2012120506A (ja) * 2010-12-10 2012-06-28 Tosoh Corp Ａｍｖ逆転写酵素遺伝子、該遺伝子によりコードされるａｍｖ逆転写酵素及び該遺伝子を用いるａｍｖ逆転写酵素の製造方法
JP2013126402A (ja) * 2011-12-19 2013-06-27 Tosoh Corp 逆転写酵素の製造方法
JP2013146235A (ja) * 2012-01-20 2013-08-01 Tosoh Corp トリ骨髄芽細胞腫ウイルス逆転写酵素の製造方法
JP6107101B2 (ja) * 2012-12-11 2017-04-05 東ソー株式会社 トリ骨髄芽細胞腫ウイルス逆転写酵素の製造方法
US10364420B2 (en) * 2015-12-07 2019-07-30 Bio-Rad Laboratories, Inc Dimeric reverse transcriptase

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4663290A (en) * 1982-01-21 1987-05-05 Molecular Genetics, Inc. Production of reverse transcriptase
US5244797B1 (en) * 1988-01-13 1998-08-25 Life Technologies Inc Cloned genes encoding reverse transcriptase lacking rnase h activity
AU696497B2 (en) * 1994-04-01 1998-09-10 Gen-Probe Incorporated Highly-purified recombinant reverse transcriptase
JP3656277B2 (ja) * 1995-05-17 2005-06-08 味の素株式会社 組換えｄｎａ法によるトランスグルタミナーゼの効率的製造法
JPH09173078A (ja) * 1995-09-14 1997-07-08 Tadayuki Imanaka 分子シャペロンを用いる蛋白質の製造方法
US5641633A (en) 1995-11-15 1997-06-24 Becton, Dickinson And Company Fluorescence polarization detection of nucleic acids
AU721695B2 (en) * 1996-08-16 2000-07-13 Dong Wha Pharmaceutical Industrial Co., Ltd. HBV polymerase, RNase H enzyme derived from HBV polymerase, processes for preparation and uses for screening antiviral agents thereof
CA2287542A1 (en) * 1997-04-22 1998-10-29 Life Technologies, Inc. Methods for the production of aslv reverse transcriptases composed of multiple subunits
JP3344618B2 (ja) * 1997-06-20 2002-11-11 株式会社エイチ・エス・ピー研究所 シャペロン発現プラスミド
JP2002534125A (ja) * 1999-01-15 2002-10-15 モレキュラー バイオロジー リソーシーズ 生物学的に活性な逆転写酵素

Also Published As

Publication number Publication date
JP2002315584A (ja) 2002-10-29
CA2357540C (en) 2010-07-13
US6902920B2 (en) 2005-06-07
ES2346511T3 (es) 2010-10-18
EP1191102B1 (de) 2010-06-16
US20020115147A1 (en) 2002-08-22
EP1191102A2 (de) 2002-03-27
CA2357540A1 (en) 2002-03-22
CN1250728C (zh) 2006-04-12
DE10046960A1 (de) 2002-04-11
EP1191102A3 (de) 2002-07-03
CN1346889A (zh) 2002-05-01
ATE471376T1 (de) 2010-07-15

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