DE60117998D1 - Mutierte furin polypeptide, welche verbesserte eigenschaften aufweisen - Google Patents

Mutierte furin polypeptide, welche verbesserte eigenschaften aufweisen

Info

Publication number
DE60117998D1
DE60117998D1 DE60117998T DE60117998T DE60117998D1 DE 60117998 D1 DE60117998 D1 DE 60117998D1 DE 60117998 T DE60117998 T DE 60117998T DE 60117998 T DE60117998 T DE 60117998T DE 60117998 D1 DE60117998 D1 DE 60117998D1
Authority
DE
Germany
Prior art keywords
furin
polypeptide
furine
mutate
improved properties
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60117998T
Other languages
English (en)
Other versions
DE60117998T2 (de
Inventor
Barbara Plaimauer
Uwe Schlokat
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baxter AG
Original Assignee
Baxter AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter AG filed Critical Baxter AG
Application granted granted Critical
Publication of DE60117998D1 publication Critical patent/DE60117998D1/de
Publication of DE60117998T2 publication Critical patent/DE60117998T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21075—Furin (3.4.21.75)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6454—Dibasic site splicing serine proteases, e.g. kexin (3.4.21.61); furin (3.4.21.75) and other proprotein convertases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE60117998T 2000-06-09 2001-05-25 Mutierte Furin-Polypeptide mit verbesserten Eigenschaften Expired - Lifetime DE60117998T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US592480 2000-06-09
US09/592,480 US6596526B1 (en) 2000-06-09 2000-06-09 Furin polypeptides with improved characteristics
PCT/EP2001/005991 WO2001094383A2 (en) 2000-06-09 2001-05-25 Mutated furin polypeptides having improved characteristics

Publications (2)

Publication Number Publication Date
DE60117998D1 true DE60117998D1 (de) 2006-05-11
DE60117998T2 DE60117998T2 (de) 2006-11-16

Family

ID=24370816

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60117998T Expired - Lifetime DE60117998T2 (de) 2000-06-09 2001-05-25 Mutierte Furin-Polypeptide mit verbesserten Eigenschaften

Country Status (9)

Country Link
US (1) US6596526B1 (de)
EP (1) EP1294864B1 (de)
JP (3) JP5591432B2 (de)
AT (1) ATE320485T1 (de)
AU (2) AU7843101A (de)
CA (1) CA2411694C (de)
DE (1) DE60117998T2 (de)
ES (1) ES2260255T3 (de)
WO (1) WO2001094383A2 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6596526B1 (en) * 2000-06-09 2003-07-22 Baxter Aktiengesellschaft Furin polypeptides with improved characteristics
EP1799244A4 (de) * 2004-07-23 2010-06-09 Smith Judith Furinhemmer
US20060094104A1 (en) 2004-10-29 2006-05-04 Leopold Grillberger Animal protein-free media for cultivation of cells
PT1974014T (pt) * 2006-01-04 2017-05-26 Baxalta Inc Meios de cultura celulares livres de oligopeptídeos
CN110066782B (zh) * 2007-12-31 2024-04-16 武田药品工业株式会社 基本上无动物蛋白的重组体弗林蛋白酶及其生产方法
US8241623B1 (en) 2009-02-09 2012-08-14 David Bermudes Protease sensitivity expression system
US20130149295A1 (en) 2010-05-12 2013-06-13 Martine Cohen-Solal Furin and biologically active derivatives thereof for use in the prevention or treatment of an inflammatory disease
US20140051832A1 (en) * 2011-03-11 2014-02-20 Universidade De São Paulo - Usp Method for the production of recombinant human factor viii
CN103889454B (zh) 2011-06-02 2017-04-05 百深有限责任公司 重组成对碱性氨基酸蛋白酶的制剂
AR102048A1 (es) * 2014-08-12 2017-02-01 Baxalta Inc Producción del factor x completamente procesado y funcional en un sistema de expresión de mamífero de secreción de furina
JP7253505B2 (ja) 2017-06-23 2023-04-06 武田薬品工業株式会社 第ｖｉｉｉ因子亜種の精製

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0465529T3 (da) 1989-03-21 1998-10-05 Vical Inc Ekspression af exogene polynukleotidsekvenser i et hvirveldyr
NL9000917A (nl) 1989-10-25 1991-05-16 Holland Biotechnology Farmaceutisch preparaat met endoproteolytische activiteit; werkwijze voor endoproteolytische processing van (precursor) eiwitten en voor de (micro) biologische bereiding van eiwitten.
DK0574402T3 (da) * 1990-11-26 1998-05-18 Chiron Corp Ekspression af PACE i værtsceller og fremgangsmåder til anvendelse deraf
US5298422A (en) 1991-11-06 1994-03-29 Baylor College Of Medicine Myogenic vector systems
AT404838B (de) * 1995-11-24 1999-03-25 Immuno Ag Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen
JPH09295945A (ja) 1996-04-30 1997-11-18 Hoechst Yakuhin Kogyo Kk 成熟型骨誘導因子の製造方法
US6596526B1 (en) * 2000-06-09 2003-07-22 Baxter Aktiengesellschaft Furin polypeptides with improved characteristics

Also Published As

Publication number Publication date
DE60117998T2 (de) 2006-11-16
ES2260255T3 (es) 2006-11-01
US6596526B1 (en) 2003-07-22
EP1294864A2 (de) 2003-03-26
JP2011182802A (ja) 2011-09-22
CA2411694C (en) 2013-11-19
CA2411694A1 (en) 2001-12-13
AU7843101A (en) 2001-12-17
JP5591432B2 (ja) 2014-09-17
EP1294864B1 (de) 2006-03-15
JP2004508015A (ja) 2004-03-18
WO2001094383A3 (en) 2002-11-21
JP2014155506A (ja) 2014-08-28
WO2001094383A2 (en) 2001-12-13
AU2001278431B2 (en) 2006-08-10
ATE320485T1 (de) 2006-04-15

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