CA2411694C - Furin polypeptides with improved characteristics - Google Patents

Furin polypeptides with improved characteristics Download PDF

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Publication number
CA2411694C
CA2411694C CA2411694A CA2411694A CA2411694C CA 2411694 C CA2411694 C CA 2411694C CA 2411694 A CA2411694 A CA 2411694A CA 2411694 A CA2411694 A CA 2411694A CA 2411694 C CA2411694 C CA 2411694C
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CA
Canada
Prior art keywords
furin
amino acids
furin polypeptide
modified
polypeptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2411694A
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English (en)
French (fr)
Other versions
CA2411694A1 (en
Inventor
Barbara Plaimauer
Uwe Schlokat
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Baxalta GmbH
Baxalta Inc
Original Assignee
Baxter AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxter AG filed Critical Baxter AG
Publication of CA2411694A1 publication Critical patent/CA2411694A1/en
Application granted granted Critical
Publication of CA2411694C publication Critical patent/CA2411694C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21075—Furin (3.4.21.75)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6454—Dibasic site splicing serine proteases, e.g. kexin (3.4.21.61); furin (3.4.21.75) and other proprotein convertases
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CA2411694A 2000-06-09 2001-05-25 Furin polypeptides with improved characteristics Expired - Lifetime CA2411694C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/592,480 US6596526B1 (en) 2000-06-09 2000-06-09 Furin polypeptides with improved characteristics
US09/592,480 2000-06-09
PCT/EP2001/005991 WO2001094383A2 (en) 2000-06-09 2001-05-25 Mutated furin polypeptides having improved characteristics

Publications (2)

Publication Number Publication Date
CA2411694A1 CA2411694A1 (en) 2001-12-13
CA2411694C true CA2411694C (en) 2013-11-19

Family

ID=24370816

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2411694A Expired - Lifetime CA2411694C (en) 2000-06-09 2001-05-25 Furin polypeptides with improved characteristics

Country Status (9)

Country Link
US (1) US6596526B1 (de)
EP (1) EP1294864B1 (de)
JP (3) JP5591432B2 (de)
AT (1) ATE320485T1 (de)
AU (2) AU7843101A (de)
CA (1) CA2411694C (de)
DE (1) DE60117998T2 (de)
ES (1) ES2260255T3 (de)
WO (1) WO2001094383A2 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6596526B1 (en) * 2000-06-09 2003-07-22 Baxter Aktiengesellschaft Furin polypeptides with improved characteristics
US8263563B2 (en) * 2004-07-23 2012-09-11 Robert E. Smith Furin inhibitors
US20060094104A1 (en) 2004-10-29 2006-05-04 Leopold Grillberger Animal protein-free media for cultivation of cells
PL2522717T3 (pl) * 2006-01-04 2014-08-29 Baxalta Inc Pożywki do hodowli komórkowych wolne od oligopeptydów
ES2735173T3 (es) * 2007-12-31 2019-12-16 Baxalta GmbH Furina recombinante sustancialmente libre de proteína animal y métodos para producir la misma
US8241623B1 (en) 2009-02-09 2012-08-14 David Bermudes Protease sensitivity expression system
EP2569005A1 (de) 2010-05-12 2013-03-20 INSERM - Institut National de la Santé et de la Recherche Médicale Furin und biologisch aktive derivate davon zur verwendung bei der vorbeugung oder behandlung einer entzündungskrankheit
WO2012122611A1 (en) * 2011-03-11 2012-09-20 Universidade De São Paulo - Usp Method for the production of recombinant human factor viii
KR102050609B1 (ko) 2011-06-02 2019-11-29 박스알타 인코퍼레이티드 재조합 퓨린의 제형
AR102048A1 (es) * 2014-08-12 2017-02-01 Baxalta Inc Producción del factor x completamente procesado y funcional en un sistema de expresión de mamífero de secreción de furina
CA3068121A1 (en) 2017-06-23 2018-12-27 Baxalta Incorporated Purification of factor viii subspecies
CN117756926B (zh) * 2023-12-26 2024-06-07 广东普言生物科技有限公司 一种重组ⅩⅦ型胶原蛋白Pro.C17及其制备方法和应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69032284T2 (de) 1989-03-21 1998-10-08 Vical, Inc., San Diego, Calif. Expression von exogenen polynukleotidsequenzen in wirbeltieren
NL9000917A (nl) 1989-10-25 1991-05-16 Holland Biotechnology Farmaceutisch preparaat met endoproteolytische activiteit; werkwijze voor endoproteolytische processing van (precursor) eiwitten en voor de (micro) biologische bereiding van eiwitten.
ATE158816T1 (de) * 1990-11-26 1997-10-15 Genetics Inst Expression von pace in wirtszellen und verfahren zu dessen verwendung
US5298422A (en) 1991-11-06 1994-03-29 Baylor College Of Medicine Myogenic vector systems
AT404838B (de) * 1995-11-24 1999-03-25 Immuno Ag Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen
JPH09295945A (ja) 1996-04-30 1997-11-18 Hoechst Yakuhin Kogyo Kk 成熟型骨誘導因子の製造方法
US6596526B1 (en) * 2000-06-09 2003-07-22 Baxter Aktiengesellschaft Furin polypeptides with improved characteristics

Also Published As

Publication number Publication date
DE60117998D1 (de) 2006-05-11
JP2014155506A (ja) 2014-08-28
JP2011182802A (ja) 2011-09-22
AU7843101A (en) 2001-12-17
DE60117998T2 (de) 2006-11-16
WO2001094383A3 (en) 2002-11-21
AU2001278431B2 (en) 2006-08-10
EP1294864A2 (de) 2003-03-26
ES2260255T3 (es) 2006-11-01
JP5591432B2 (ja) 2014-09-17
CA2411694A1 (en) 2001-12-13
US6596526B1 (en) 2003-07-22
ATE320485T1 (de) 2006-04-15
WO2001094383A2 (en) 2001-12-13
JP2004508015A (ja) 2004-03-18
EP1294864B1 (de) 2006-03-15

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Effective date: 20210525