AR102048A1 - Producción del factor x completamente procesado y funcional en un sistema de expresión de mamífero de secreción de furina - Google Patents

Producción del factor x completamente procesado y funcional en un sistema de expresión de mamífero de secreción de furina

Info

Publication number
AR102048A1
AR102048A1 ARP150102587A ARP150102587A AR102048A1 AR 102048 A1 AR102048 A1 AR 102048A1 AR P150102587 A ARP150102587 A AR P150102587A AR P150102587 A ARP150102587 A AR P150102587A AR 102048 A1 AR102048 A1 AR 102048A1
Authority
AR
Argentina
Prior art keywords
factor
furina
secretion
expression system
functional
Prior art date
Application number
ARP150102587A
Other languages
English (en)
Inventor
Dockal Michael
Koehn Jadranka
Horling Franziska
Bhm Ernst
Original Assignee
Baxalta Inc
Baxalta GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxalta Inc, Baxalta GmbH filed Critical Baxalta Inc
Publication of AR102048A1 publication Critical patent/AR102048A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21006—Coagulation factor Xa (3.4.21.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • A61K38/482—Serine endopeptidases (3.4.21)
    • A61K38/4846—Factor VII (3.4.21.21); Factor IX (3.4.21.22); Factor Xa (3.4.21.6); Factor XI (3.4.21.27); Factor XII (3.4.21.38)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/04—Antihaemorrhagics; Procoagulants; Haemostatic agents; Antifibrinolytic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6432—Coagulation factor Xa (3.4.21.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6454—Dibasic site splicing serine proteases, e.g. kexin (3.4.21.61); furin (3.4.21.75) and other proprotein convertases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21075—Furin (3.4.21.75)

Abstract

En la presente se revelan métodos para la producción del Factor X maduro completamente procesado en un sistema de expresión que produce una cantidad controlada de furina entre 50 U/mL y 300 U/mL del sobrenadante de cultivo. También se revelan. células transformadas, sistemas de expresión y vectores de expresión para la expresión de furina y el Factor X.
ARP150102587A 2014-08-12 2015-08-11 Producción del factor x completamente procesado y funcional en un sistema de expresión de mamífero de secreción de furina AR102048A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201462036438P 2014-08-12 2014-08-12

Publications (1)

Publication Number Publication Date
AR102048A1 true AR102048A1 (es) 2017-02-01

Family

ID=54035298

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP150102587A AR102048A1 (es) 2014-08-12 2015-08-11 Producción del factor x completamente procesado y funcional en un sistema de expresión de mamífero de secreción de furina

Country Status (14)

Country Link
US (1) US9873892B2 (es)
EP (1) EP3180427B1 (es)
JP (1) JP6793114B2 (es)
CN (1) CN107002041B (es)
AR (1) AR102048A1 (es)
AU (1) AU2015301726B2 (es)
CA (1) CA2958056C (es)
EA (1) EA035444B1 (es)
ES (1) ES2742724T3 (es)
IL (1) IL250530B (es)
MX (1) MX2017001931A (es)
NZ (1) NZ729729A (es)
TW (1) TWI666319B (es)
WO (1) WO2016025615A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8241623B1 (en) 2009-02-09 2012-08-14 David Bermudes Protease sensitivity expression system
CA3068121A1 (en) 2017-06-23 2018-12-27 Baxalta Incorporated Purification of factor viii subspecies
WO2020257942A1 (en) * 2019-06-28 2020-12-30 Peel Sean A F A method for increasing yields and the specific activity of certain recombinant proteins in mammalian cells by co-expressing full-length furin
CN115298204B (zh) * 2022-01-25 2023-04-25 青岛万明赛伯药业有限公司 proNGF突变体及其用途

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992009698A1 (en) * 1990-11-26 1992-06-11 Genetics Institute, Inc. Expression of pace in host cells and methods of use thereof
AT404838B (de) 1995-11-24 1999-03-25 Immuno Ag Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen
US5876923A (en) 1996-07-26 1999-03-02 Arch Development Corporation Herpes simplex virus ICP4 as an inhibitor of apoptosis
EP2292657A1 (en) 1999-11-12 2011-03-09 Baxter Biotech Technology S.A.R.L. Reduced side-effect hemoglobin compositions
US6596526B1 (en) * 2000-06-09 2003-07-22 Baxter Aktiengesellschaft Furin polypeptides with improved characteristics
US7223390B2 (en) * 2003-05-09 2007-05-29 Research Development Foundation Insertion of furin protease cleavage sites in membrane proteins and uses thereof
PL2152735T3 (pl) * 2007-05-18 2015-08-31 Baxalta Inc Sposób wytwarzania dojrzałego VWF z propeptydu
PT2240578T (pt) * 2007-12-31 2019-06-19 Baxalta GmbH Furina recombinante substancialmente isenta de proteínas animais e métodos para a sua produção
CN102348715B (zh) 2009-02-03 2017-12-08 阿穆尼克斯运营公司 延伸重组多肽和包含该延伸重组多肽的组合物
AR086672A1 (es) * 2011-06-02 2014-01-15 Baxter Int Formulaciones de furina recombinante

Also Published As

Publication number Publication date
CN107002041A (zh) 2017-08-01
EP3180427B1 (en) 2019-05-22
EP3180427A1 (en) 2017-06-21
CA2958056C (en) 2023-07-04
EA035444B1 (ru) 2020-06-17
ES2742724T3 (es) 2020-02-17
IL250530B (en) 2020-03-31
CN107002041B (zh) 2021-06-29
JP6793114B2 (ja) 2020-12-09
NZ729729A (en) 2023-07-28
IL250530A0 (en) 2017-03-30
US9873892B2 (en) 2018-01-23
JP2017524366A (ja) 2017-08-31
TWI666319B (zh) 2019-07-21
CA2958056A1 (en) 2016-02-18
EA201790360A1 (ru) 2017-06-30
AU2015301726B2 (en) 2021-10-21
TW201625791A (zh) 2016-07-16
WO2016025615A1 (en) 2016-02-18
MX2017001931A (es) 2017-06-30
AU2015301726A1 (en) 2017-03-16
US20160046957A1 (en) 2016-02-18

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