ES2573958B1 - Bacteria modificada genéticamente deficiente en la asimilación de alcoholes - Google Patents

Bacteria modificada genéticamente deficiente en la asimilación de alcoholes Download PDF

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Publication number
ES2573958B1
ES2573958B1 ES201431826A ES201431826A ES2573958B1 ES 2573958 B1 ES2573958 B1 ES 2573958B1 ES 201431826 A ES201431826 A ES 201431826A ES 201431826 A ES201431826 A ES 201431826A ES 2573958 B1 ES2573958 B1 ES 2573958B1
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Spain
Prior art keywords
alcohols
genetically modified
assimilation
deficient
modified bacteria
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Expired - Fee Related
Application number
ES201431826A
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ES2573958A1 (es
Inventor
María Rosario GÓMEZ GARCÍA
María Del Sol CUENCA MARTÍN
Zulema UDAONDO DOMÍNGUEZ
Amalia ROCA HERNÁNDEZ
Juan Luis RAMOS MARTÍN
María Estrella DUQUE MARTÍN DE OLIVA
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Abengoa Research SL
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Abengoa Research SL
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Priority to ES201431826A priority Critical patent/ES2573958B1/es
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Publication of ES2573958B1 publication Critical patent/ES2573958B1/es
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16—Butanols
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/38—Pseudomonas
    • C12R2001/40—Pseudomonas putida

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Bacteria modificada genéticamente deficiente en la asimilación de alcoholes.#La presente invención se refiere a una célula bacteriana genéticamente modificada deficiente en la asimilación de alcoholes como fuente de carbono respecto a la cepa silvestre de la cual proviene. La invención también se relaciona con un método para producir dicha célula y a una composición microbiológica para producir alcoholes. Asimismo, la invención se relaciona con el uso de las células bacterianas modificadas genéticamente o de la composición microbiológica para producir alcoholes así como a un procedimiento para producir alcoholes a partir de dichas células o de dicha composición microbiológica.

Description

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Claims (1)

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ES201431826A 2014-12-12 2014-12-12 Bacteria modificada genéticamente deficiente en la asimilación de alcoholes Expired - Fee Related ES2573958B1 (es)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES201431826A ES2573958B1 (es) 2014-12-12 2014-12-12 Bacteria modificada genéticamente deficiente en la asimilación de alcoholes

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201431826A ES2573958B1 (es) 2014-12-12 2014-12-12 Bacteria modificada genéticamente deficiente en la asimilación de alcoholes

Publications (2)

Publication Number Publication Date
ES2573958A1 ES2573958A1 (es) 2016-06-13
ES2573958B1 true ES2573958B1 (es) 2017-03-28

Family

ID=56098520

Family Applications (1)

Application Number Title Priority Date Filing Date
ES201431826A Expired - Fee Related ES2573958B1 (es) 2014-12-12 2014-12-12 Bacteria modificada genéticamente deficiente en la asimilación de alcoholes

Country Status (1)

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ES (1) ES2573958B1 (es)

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7842485B2 (en) * 2006-03-15 2010-11-30 The Regents Of The University Of Colorado Enhanced alcohol tolerant microorganism and methods of use thereof
US7659104B2 (en) * 2006-05-05 2010-02-09 E.I. Du Pont De Nemours And Company Solvent tolerant microorganisms and methods of isolation
WO2009143455A2 (en) * 2008-05-22 2009-11-26 Microbia, Inc. Engineering resistance to aliphatic alcohols

Also Published As

Publication number Publication date
ES2573958A1 (es) 2016-06-13

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Effective date: 20210929