DE60104810D1 - Zellfreies Verfahren zur Herstellung von posttranslational veränderten Proteinen - Google Patents

Zellfreies Verfahren zur Herstellung von posttranslational veränderten Proteinen

Info

Publication number
DE60104810D1
DE60104810D1 DE60104810T DE60104810T DE60104810D1 DE 60104810 D1 DE60104810 D1 DE 60104810D1 DE 60104810 T DE60104810 T DE 60104810T DE 60104810 T DE60104810 T DE 60104810T DE 60104810 D1 DE60104810 D1 DE 60104810D1
Authority
DE
Germany
Prior art keywords
post
cell
production
modified proteins
translationally modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE60104810T
Other languages
English (en)
Other versions
DE60104810T2 (de
Inventor
Cha Yong Choi
Sang Hyeon Kang
Taek Jin Kang
Ji Hyoung Woo
Sang Kil Lee
Seung Woo Cho
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DreamBiogen Co Ltd
Original Assignee
DreamBiogen Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DreamBiogen Co Ltd filed Critical DreamBiogen Co Ltd
Publication of DE60104810D1 publication Critical patent/DE60104810D1/de
Application granted granted Critical
Publication of DE60104810T2 publication Critical patent/DE60104810T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE60104810T 2001-02-07 2001-02-22 Zellfreies Verfahren zur Herstellung von posttranslational veränderten Proteinen Expired - Fee Related DE60104810T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2001-0005949A KR100399337B1 (ko) 2001-02-07 2001-02-07 단백질의 무세포 번역후수식법
KR2001005949 2001-02-07

Publications (2)

Publication Number Publication Date
DE60104810D1 true DE60104810D1 (de) 2004-09-16
DE60104810T2 DE60104810T2 (de) 2005-09-15

Family

ID=19705454

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60104810T Expired - Fee Related DE60104810T2 (de) 2001-02-07 2001-02-22 Zellfreies Verfahren zur Herstellung von posttranslational veränderten Proteinen

Country Status (6)

Country Link
US (2) US6780607B2 (de)
EP (1) EP1231278B1 (de)
JP (1) JP2002238595A (de)
KR (1) KR100399337B1 (de)
DE (1) DE60104810T2 (de)
ES (1) ES2227097T3 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2686899B1 (fr) * 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
KR100399337B1 (ko) * 2001-02-07 2003-09-26 드림바이오젠 주식회사 단백질의 무세포 번역후수식법
AU2002349296A1 (en) * 2001-11-30 2003-06-10 Novozymes A/S Method for generating a site-specific library of variants
CA2471363C (en) 2001-12-21 2014-02-11 Human Genome Sciences, Inc. Albumin fusion proteins
JP4441170B2 (ja) * 2002-11-28 2010-03-31 独立行政法人理化学研究所 Ｓ１２リボソームタンパク質に変異を有する大腸菌細胞抽出液及びそれを用いる無細胞系によるタンパク質の製造方法
JP4389870B2 (ja) * 2003-06-10 2009-12-24 株式会社島津製作所 哺乳動物培養細胞抽出液を用いた無細胞系タンパク質合成方法
JP4091901B2 (ja) * 2003-11-13 2008-05-28 株式会社島津製作所 無細胞系タンパク質合成におけるミクロソーム膜添加による翻訳後修飾方法
JP3965437B2 (ja) * 2003-11-14 2007-08-29 国立大学法人山口大学 無細胞系タンパク質合成におけるミクロソーム膜添加による翻訳後修飾方法
WO2005075660A1 (ja) * 2004-02-03 2005-08-18 Toyo Boseki Kabushiki Kaisha 改良された無細胞タンパク質合成用組成物
FR2870259A1 (fr) * 2004-05-12 2005-11-18 Univ D Aix Marseille I Procede de preparation de composes osidiques et des kits pour sa mise en oeuvre
JP4590249B2 (ja) * 2004-11-17 2010-12-01 独立行政法人理化学研究所 糖タンパク質合成用の無細胞タンパク質合成システム
JP4868731B2 (ja) 2004-11-17 2012-02-01 独立行政法人理化学研究所 哺乳動物培養細胞由来の無細胞タンパク質合成システム
JP4787488B2 (ja) * 2004-11-19 2011-10-05 独立行政法人理化学研究所 直鎖状鋳型ｄｎａを用いた無細胞タンパク質合成方法及びそのための細胞抽出液
US20060211083A1 (en) * 2005-01-21 2006-09-21 Federico Katzen Products and processes for in vitro synthesis of biomolecules
WO2007120614A2 (en) * 2006-04-11 2007-10-25 Sloan-Kettering Institute For Cancer Research Homogeneous erythropoietin and other peptides and proteins, methods and intermediates for their preparation
US7610567B2 (en) * 2006-04-27 2009-10-27 Achronix Semiconductor Corporation Systems and methods for performing automated conversion of representations of synchronous circuit designs to and from representations of asynchronous circuit designs
JP5704677B2 (ja) * 2007-11-05 2015-04-22 独立行政法人理化学研究所 膜タンパク質の製造方法
DE102008054716A1 (de) * 2008-12-16 2010-06-17 Evonik Degussa Gmbh Inprozesskontrolle in einem Verfahren zur Herstellung von EPO
CN114891855A (zh) 2013-12-06 2022-08-12 哈佛大学校长及研究员协会 基于纸的合成基因网络
EP3094639A4 (de) * 2014-01-13 2017-08-30 Magdalena Leszcyniecka Verfahren zur optimierung der posttranslationalen modifikationen auf rekombinanten proteinen
US9908064B2 (en) 2015-02-06 2018-03-06 Leidos, Inc. Portable fluidic platform for rapid cell-free production of protein biologics
WO2017022696A1 (ja) 2015-07-31 2017-02-09 国立研究開発法人理化学研究所 膜タンパク質の製造方法およびその利用
MX2021000102A (es) 2018-07-03 2021-05-31 Leidos Inc Materiales y metodos para expresion sin celulas de concatemeros de epitopos de vacuna.
CN111484998B (zh) * 2019-05-30 2023-04-21 康码（上海）生物科技有限公司 体外定量共表达多种蛋白的方法及其应用
WO2023152518A1 (en) * 2022-02-14 2023-08-17 Nuclera Ltd Methods for optimizing cell free protein synthesis reagents

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ210501A (en) * 1983-12-13 1991-08-27 Kirin Amgen Inc Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence
ES2097363T3 (es) 1991-10-11 1997-04-01 Promega Corp Transcripcion y traduccion acopladas en un extracto exento de celulas eucariotas.
US6103489A (en) * 1997-03-21 2000-08-15 University Of Hawaii Cell-free protein synthesis system with protein translocation and processing
CA2364921A1 (en) * 1999-02-22 2000-08-31 European Molecular Biology Laboratory Translation system
KR100399337B1 (ko) * 2001-02-07 2003-09-26 드림바이오젠 주식회사 단백질의 무세포 번역후수식법

Also Published As

Publication number Publication date
US6780607B2 (en) 2004-08-24
KR20020065769A (ko) 2002-08-14
EP1231278A1 (de) 2002-08-14
ES2227097T3 (es) 2005-04-01
KR100399337B1 (ko) 2003-09-26
US20050084926A1 (en) 2005-04-21
JP2002238595A (ja) 2002-08-27
DE60104810T2 (de) 2005-09-15
US20020106719A1 (en) 2002-08-08
EP1231278B1 (de) 2004-08-11

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee