DE4102594C2 - - Google Patents

Info

Publication number
DE4102594C2
DE4102594C2 DE19914102594 DE4102594A DE4102594C2 DE 4102594 C2 DE4102594 C2 DE 4102594C2 DE 19914102594 DE19914102594 DE 19914102594 DE 4102594 A DE4102594 A DE 4102594A DE 4102594 C2 DE4102594 C2 DE 4102594C2
Authority
DE
Germany
Prior art keywords
dna sequence
promoter
escherichia coli
nri
expression
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE19914102594
Other languages
German (de)
English (en)
Other versions
DE4102594A1 (de
Inventor
Volker Dr. O-6900 Jena De Schroeckh
John E. G. Dr. 3300 Braunschweig De Maccarthy
Dieter Dr. O-6900 Jena De Riesenberg
Wolf-Dieter Prof. Dr. 3300 Braunschweig De Deckwer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Helmholtz Zentrum fuer Infektionsforschung HZI GmbH
Original Assignee
GESELLSCHAFT fur BIOTECHNOLOGISCHE FORSCHUNG MBH (GBF) 3300 BRAUNSCHWEIG DE
INSTITUT fur MIKROBIOLOGIE und EXPERIMENTELLE THERAPIE O-6900 JENA DE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GESELLSCHAFT fur BIOTECHNOLOGISCHE FORSCHUNG MBH (GBF) 3300 BRAUNSCHWEIG DE, INSTITUT fur MIKROBIOLOGIE und EXPERIMENTELLE THERAPIE O-6900 JENA DE filed Critical GESELLSCHAFT fur BIOTECHNOLOGISCHE FORSCHUNG MBH (GBF) 3300 BRAUNSCHWEIG DE
Priority to DE19914102594 priority Critical patent/DE4102594A1/de
Publication of DE4102594A1 publication Critical patent/DE4102594A1/de
Application granted granted Critical
Publication of DE4102594C2 publication Critical patent/DE4102594C2/de
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8114—Kunitz type inhibitors
    • C07K14/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DE19914102594 1991-01-29 1991-01-29 Verfahren zur expression rekombinanter gene unter stickstoffmangel-bedingungen Granted DE4102594A1 (de)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DE19914102594 DE4102594A1 (de) 1991-01-29 1991-01-29 Verfahren zur expression rekombinanter gene unter stickstoffmangel-bedingungen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19914102594 DE4102594A1 (de) 1991-01-29 1991-01-29 Verfahren zur expression rekombinanter gene unter stickstoffmangel-bedingungen

Publications (2)

Publication Number Publication Date
DE4102594A1 DE4102594A1 (de) 1992-07-30
DE4102594C2 true DE4102594C2 (fr) 1993-07-22

Family

ID=6423929

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19914102594 Granted DE4102594A1 (de) 1991-01-29 1991-01-29 Verfahren zur expression rekombinanter gene unter stickstoffmangel-bedingungen

Country Status (1)

Country Link
DE (1) DE4102594A1 (fr)

Also Published As

Publication number Publication date
DE4102594A1 (de) 1992-07-30

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Legal Events

Date Code Title Description
OP8 Request for examination as to paragraph 44 patent law
D2 Grant after examination
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: GESELLSCHAFT FUER BIOTECHNOLOGISCHE FORSCHUNG MBH

8339 Ceased/non-payment of the annual fee