DE3821407A1 - Immunoassay-verfahren und reagenzien zur durchfuehrung des verfahrens - Google Patents
Immunoassay-verfahren und reagenzien zur durchfuehrung des verfahrensInfo
- Publication number
- DE3821407A1 DE3821407A1 DE19883821407 DE3821407A DE3821407A1 DE 3821407 A1 DE3821407 A1 DE 3821407A1 DE 19883821407 DE19883821407 DE 19883821407 DE 3821407 A DE3821407 A DE 3821407A DE 3821407 A1 DE3821407 A1 DE 3821407A1
- Authority
- DE
- Germany
- Prior art keywords
- microcapsules
- antigen
- reagent
- antibody
- salt
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000003018 immunoassay Methods 0.000 title claims abstract description 29
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 36
- 238000000034 method Methods 0.000 title claims description 7
- 239000003094 microcapsule Substances 0.000 claims abstract description 70
- ZLLMYPVFZJLNKV-UHFFFAOYSA-M sodium 3-[3-hydroxy-4-[(5-nitropyridin-2-yl)diazenyl]-N-propylanilino]propane-1-sulfonate Chemical compound [Na+].CCCN(CCCS([O-])(=O)=O)c1ccc(N=Nc2ccc(cn2)[N+]([O-])=O)c(O)c1 ZLLMYPVFZJLNKV-UHFFFAOYSA-M 0.000 claims abstract description 33
- 239000000427 antigen Substances 0.000 claims abstract description 32
- 108091007433 antigens Proteins 0.000 claims abstract description 32
- 102000036639 antigens Human genes 0.000 claims abstract description 32
- 239000012528 membrane Substances 0.000 claims abstract description 27
- 210000004369 blood Anatomy 0.000 claims abstract description 6
- 239000008280 blood Substances 0.000 claims abstract description 6
- 229910021645 metal ion Inorganic materials 0.000 claims description 24
- 230000009089 cytolysis Effects 0.000 claims description 15
- 150000003839 salts Chemical class 0.000 claims description 15
- 239000007795 chemical reaction product Substances 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 14
- 239000006185 dispersion Substances 0.000 claims description 5
- 239000000047 product Substances 0.000 claims description 5
- 159000000007 calcium salts Chemical class 0.000 claims description 4
- 239000002502 liposome Substances 0.000 claims description 4
- 150000002500 ions Chemical class 0.000 claims description 3
- 159000000003 magnesium salts Chemical class 0.000 claims description 3
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- 230000031700 light absorption Effects 0.000 claims 1
- 230000035945 sensitivity Effects 0.000 abstract description 4
- 239000002738 chelating agent Substances 0.000 description 13
- 238000006243 chemical reaction Methods 0.000 description 13
- 239000000243 solution Substances 0.000 description 10
- 238000010521 absorption reaction Methods 0.000 description 8
- 230000000295 complement effect Effects 0.000 description 8
- 230000036046 immunoreaction Effects 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- 238000011088 calibration curve Methods 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 4
- 229910001429 cobalt ion Inorganic materials 0.000 description 4
- XLJKHNWPARRRJB-UHFFFAOYSA-N cobalt(2+) Chemical compound [Co+2] XLJKHNWPARRRJB-UHFFFAOYSA-N 0.000 description 4
- 238000004108 freeze drying Methods 0.000 description 4
- 150000002632 lipids Chemical class 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 3
- 230000009918 complex formation Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000011541 reaction mixture Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- XUIIKFGFIJCVMT-GFCCVEGCSA-N D-thyroxine Chemical compound IC1=CC(C[C@@H](N)C(O)=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-GFCCVEGCSA-N 0.000 description 2
- 102000001554 Hemoglobins Human genes 0.000 description 2
- 108010054147 Hemoglobins Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000008033 biological extinction Effects 0.000 description 2
- 239000000356 contaminant Substances 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 229940034208 thyroxine Drugs 0.000 description 2
- XUIIKFGFIJCVMT-UHFFFAOYSA-N thyroxine-binding globulin Natural products IC1=CC(CC([NH3+])C([O-])=O)=CC(I)=C1OC1=CC(I)=C(O)C(I)=C1 XUIIKFGFIJCVMT-UHFFFAOYSA-N 0.000 description 2
- MLIWQXBKMZNZNF-KUHOPJCQSA-N (2e)-2,6-bis[(4-azidophenyl)methylidene]-4-methylcyclohexan-1-one Chemical compound O=C1\C(=C\C=2C=CC(=CC=2)N=[N+]=[N-])CC(C)CC1=CC1=CC=C(N=[N+]=[N-])C=C1 MLIWQXBKMZNZNF-KUHOPJCQSA-N 0.000 description 1
- BZRWWAYVITZYRZ-UHFFFAOYSA-N 3-[4-[(5-bromopyridin-2-yl)diazenyl]-3-hydroxy-N-propylanilino]propane-1-sulfonic acid Chemical compound OC1=CC(N(CCCS(O)(=O)=O)CCC)=CC=C1N=NC1=CC=C(Br)C=N1 BZRWWAYVITZYRZ-UHFFFAOYSA-N 0.000 description 1
- -1 5-nitro-2-pyridylazo Chemical group 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical class [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000013522 chelant Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 238000010668 complexation reaction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- HZKQOZMTXBKNAP-UHFFFAOYSA-L disodium 3-[3-hydroxy-4-[(5-nitropyridin-2-yl)diazenyl]-N-propylanilino]propane-1-sulfonate Chemical compound [Na+].[Na+].CCCN(CCCS([O-])(=O)=O)c1ccc(N=Nc2ccc(cn2)[N+]([O-])=O)c(O)c1.CCCN(CCCS([O-])(=O)=O)c1ccc(N=Nc2ccc(cn2)[N+]([O-])=O)c(O)c1 HZKQOZMTXBKNAP-UHFFFAOYSA-L 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 239000008384 inner phase Substances 0.000 description 1
- 239000000644 isotonic solution Substances 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 235000001055 magnesium Nutrition 0.000 description 1
- 229940091250 magnesium supplement Drugs 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 229960003742 phenol Drugs 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 159000000000 sodium salts Chemical class 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 239000012798 spherical particle Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 201000009482 yaws Diseases 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/5432—Liposomes or microcapsules
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
- G01N33/586—Liposomes, microcapsules or cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP15770187A JPS643563A (en) | 1987-06-26 | 1987-06-26 | Reagent for immunoassay |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3821407A1 true DE3821407A1 (de) | 1989-01-05 |
| DE3821407C2 DE3821407C2 (https=) | 1992-07-02 |
Family
ID=15655488
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19883821407 Granted DE3821407A1 (de) | 1987-06-26 | 1988-06-24 | Immunoassay-verfahren und reagenzien zur durchfuehrung des verfahrens |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JPS643563A (https=) |
| DE (1) | DE3821407A1 (https=) |
-
1987
- 1987-06-26 JP JP15770187A patent/JPS643563A/ja active Pending
-
1988
- 1988-06-24 DE DE19883821407 patent/DE3821407A1/de active Granted
Non-Patent Citations (1)
| Title |
|---|
| Patents Abstracts of Japan, P-531 December 26, 1986, Vol. 10/No. 389 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS643563A (en) | 1989-01-09 |
| DE3821407C2 (https=) | 1992-07-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OP8 | Request for examination as to paragraph 44 patent law | ||
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |