JPS643563A - Reagent for immunoassay - Google Patents

Reagent for immunoassay

Info

Publication number
JPS643563A
JPS643563A JP15770187A JP15770187A JPS643563A JP S643563 A JPS643563 A JP S643563A JP 15770187 A JP15770187 A JP 15770187A JP 15770187 A JP15770187 A JP 15770187A JP S643563 A JPS643563 A JP S643563A
Authority
JP
Japan
Prior art keywords
paps
nitro
endoplasmic reticulum
metal ions
soln
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP15770187A
Other languages
Japanese (ja)
Inventor
Masumi Suzuki
Kyoko Imai
Yasushi Nomura
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hitachi Ltd
Original Assignee
Hitachi Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Ltd filed Critical Hitachi Ltd
Priority to JP15770187A priority Critical patent/JPS643563A/en
Priority to DE19883821407 priority patent/DE3821407A1/en
Publication of JPS643563A publication Critical patent/JPS643563A/en
Pending legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/5432—Liposomes or microcapsules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/586—Liposomes, microcapsules or cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

PURPOSE:To improve measurement sensitivity by housing a soln. of nitro PAPS into an endoplasmic reticulum having a membrane labeled by an antigen or antibody. CONSTITUTION:This reagent has preferably the endoplasmic reticulum which has the membrane labeled with the antigen or antibody and is housed internally with the soln. of the nitro PAPS and a liquid in which this endoplasmic reticulum is dispersed and contains the metal ions capable of forming a colorable complex between the same and the nitro PAPS. The metal ions include Ni(II), Co(III), Fe(II), Zn(II), Cu(II), etc. The nitro PAPS has high water solubility, can be dissolved at several hundreds millimol or above in 1l buffer soln. and is capable of increasing the concn. of the chelate agent housed in the endoplasmic reticulum. The complex forming reaction between the nitro PAPS and the metal ions is sensitive and the coefft. of molecular absorption is as high as order of 10<5>. Since the absorption wavelength of this complex exists mostly at >=600nm, the errors by the absorption of extraneous matter are decreased. The measurement sensitivity is thus improved.
JP15770187A 1987-06-26 1987-06-26 Reagent for immunoassay Pending JPS643563A (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP15770187A JPS643563A (en) 1987-06-26 1987-06-26 Reagent for immunoassay
DE19883821407 DE3821407A1 (en) 1987-06-26 1988-06-24 Immunoassay method and reagents for carrying out the method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP15770187A JPS643563A (en) 1987-06-26 1987-06-26 Reagent for immunoassay

Publications (1)

Publication Number Publication Date
JPS643563A true JPS643563A (en) 1989-01-09

Family

ID=15655488

Family Applications (1)

Application Number Title Priority Date Filing Date
JP15770187A Pending JPS643563A (en) 1987-06-26 1987-06-26 Reagent for immunoassay

Country Status (2)

Country Link
JP (1) JPS643563A (en)
DE (1) DE3821407A1 (en)

Also Published As

Publication number Publication date
DE3821407A1 (en) 1989-01-05
DE3821407C2 (en) 1992-07-02

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