DE3810462A1 - Verfahren zum aufschluss von zellen in einer zellsuspension und vorrichtung zur durchfuehrung des verfahrens - Google Patents
Verfahren zum aufschluss von zellen in einer zellsuspension und vorrichtung zur durchfuehrung des verfahrensInfo
- Publication number
- DE3810462A1 DE3810462A1 DE3810462A DE3810462A DE3810462A1 DE 3810462 A1 DE3810462 A1 DE 3810462A1 DE 3810462 A DE3810462 A DE 3810462A DE 3810462 A DE3810462 A DE 3810462A DE 3810462 A1 DE3810462 A1 DE 3810462A1
- Authority
- DE
- Germany
- Prior art keywords
- cell suspension
- nozzles
- digestion
- feed pump
- another
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims description 18
- 239000006285 cell suspension Substances 0.000 title claims description 11
- 230000029087 digestion Effects 0.000 claims description 21
- 238000003860 storage Methods 0.000 claims description 2
- 238000010992 reflux Methods 0.000 claims 1
- 238000010079 rubber tapping Methods 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 9
- 239000000725 suspension Substances 0.000 description 9
- 230000003834 intracellular effect Effects 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108010036781 Fumarate Hydratase Proteins 0.000 description 3
- 102100036160 Fumarate hydratase, mitochondrial Human genes 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 238000000265 homogenisation Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 2
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 2
- 239000007884 disintegrant Substances 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000002604 ultrasonography Methods 0.000 description 2
- 238000001238 wet grinding Methods 0.000 description 2
- GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 description 1
- 102000013563 Acid Phosphatase Human genes 0.000 description 1
- 108010051457 Acid Phosphatase Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 101100283604 Caenorhabditis elegans pigk-1 gene Proteins 0.000 description 1
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 1
- 101100130497 Drosophila melanogaster Mical gene Proteins 0.000 description 1
- 101100345589 Mus musculus Mical1 gene Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 238000011072 cell harvest Methods 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 238000004182 chemical digestion Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000005515 coenzyme Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- VHJLVAABSRFDPM-ZXZARUISSA-N dithioerythritol Chemical compound SC[C@H](O)[C@H](O)CS VHJLVAABSRFDPM-ZXZARUISSA-N 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000017525 heat dissipation Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 238000011045 prefiltration Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
- C12N1/066—Lysis of microorganisms by physical methods
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
- B01F25/20—Jet mixers, i.e. mixers using high-speed fluid streams
- B01F25/23—Mixing by intersecting jets
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/06—Mixing of food ingredients
- B01F2101/07—Mixing ingredients into milk or cream, e.g. aerating
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/44—Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
- B01F23/40—Mixing liquids with liquids; Emulsifying
- B01F23/41—Emulsifying
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
- B01F25/50—Circulation mixers, e.g. wherein at least part of the mixture is discharged from and reintroduced into a receptacle
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Mycology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Disintegrating Or Milling (AREA)
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3810462A DE3810462A1 (de) | 1988-03-26 | 1988-03-26 | Verfahren zum aufschluss von zellen in einer zellsuspension und vorrichtung zur durchfuehrung des verfahrens |
| EP89104681A EP0335189A1 (de) | 1988-03-26 | 1989-03-16 | Verfahren zum Homogenisieren von Emulsionen und Vorrichtung zur Durchführung des Verfahrens |
| EP89104680A EP0335188A1 (de) | 1988-03-26 | 1989-03-16 | Verfahren zum Aufschluss von Zellen in einer Zellsuspension und Vorrichtung zur Durchführung des Verfahrens |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3810462A DE3810462A1 (de) | 1988-03-26 | 1988-03-26 | Verfahren zum aufschluss von zellen in einer zellsuspension und vorrichtung zur durchfuehrung des verfahrens |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3810462A1 true DE3810462A1 (de) | 1989-10-05 |
| DE3810462C2 DE3810462C2 (cg-RX-API-DMAC7.html) | 1990-02-08 |
Family
ID=6350870
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE3810462A Granted DE3810462A1 (de) | 1988-03-26 | 1988-03-26 | Verfahren zum aufschluss von zellen in einer zellsuspension und vorrichtung zur durchfuehrung des verfahrens |
Country Status (2)
| Country | Link |
|---|---|
| EP (2) | EP0335188A1 (cg-RX-API-DMAC7.html) |
| DE (1) | DE3810462A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW426733B (en) * | 1995-10-06 | 2001-03-21 | Merck & Co Inc | Method of disrupting cultured cells using an impinging jet device |
| US6135628A (en) * | 1995-10-13 | 2000-10-24 | Boehringer Ingelheim Pharmceuticals, Inc. | Method and apparatus for homogenizing aerosol formulations |
| US5721120A (en) * | 1996-10-02 | 1998-02-24 | Merck & Co., Inc. | Method of disrupting cultured cells using an impinging jet device |
| DE19749735C1 (de) * | 1997-11-11 | 2000-02-10 | Invent Gmbh Entwicklung Neuer Technologien | Verfahren und Vorrichtung zur Herstellung von Enzymen |
| US6843968B2 (en) * | 2000-09-29 | 2005-01-18 | Seiji Kagawa | Method of manufacturing liquid medium containing composite ultrafine particles and apparatus thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3230289A1 (de) * | 1982-08-14 | 1984-02-16 | Bayer Ag, 5090 Leverkusen | Herstellung von pharmazeutischen oder kosmetischen dispersionen |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR346862A (fr) * | 1904-10-07 | 1905-02-13 | Henri Charles Empis | Extraction et préparation des substances contenues dans les cellules organiques |
| US2751335A (en) * | 1951-02-01 | 1956-06-19 | Exxon Research Engineering Co | Method and apparatus for mixing and contacting fluids |
| US2751425A (en) * | 1951-02-01 | 1956-06-19 | Exxon Research Engineering Co | Method and apparatus for mixing and contacting fluids |
| US2976024A (en) * | 1954-10-06 | 1961-03-21 | Pure Oil Co | Apparatus for preparing colloidal dispersions |
| US3391908A (en) * | 1966-03-28 | 1968-07-09 | Exxon Research Engineering Co | Variable flow opposed jet mixer |
| JPS5915005B2 (ja) * | 1979-10-17 | 1984-04-07 | コニカ株式会社 | 分散方法 |
| US4533254A (en) * | 1981-04-17 | 1985-08-06 | Biotechnology Development Corporation | Apparatus for forming emulsions |
-
1988
- 1988-03-26 DE DE3810462A patent/DE3810462A1/de active Granted
-
1989
- 1989-03-16 EP EP89104680A patent/EP0335188A1/de not_active Withdrawn
- 1989-03-16 EP EP89104681A patent/EP0335189A1/de not_active Withdrawn
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3230289A1 (de) * | 1982-08-14 | 1984-02-16 | Bayer Ag, 5090 Leverkusen | Herstellung von pharmazeutischen oder kosmetischen dispersionen |
Non-Patent Citations (3)
| Title |
|---|
| Handbuch der Biotechnologie, herausgegeben von Paul Präve et al., R. Oldenbourg Verlag München, Wien 1987, S.238-241 * |
| KULA-SCHÜTTE: Aufschluß von Mikroorganismen in GVC-Vortragstagung Verfahrenstechnik der Auf- arbeitung von Bioprodukten, Düsseldorf, 14./ 15. Oktober 1985, S. 43 ff * |
| Prospekt "APV Gaulin Hochdrucktechnik" * |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0335189A1 (de) | 1989-10-04 |
| DE3810462C2 (cg-RX-API-DMAC7.html) | 1990-02-08 |
| EP0335188A1 (de) | 1989-10-04 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OP8 | Request for examination as to paragraph 44 patent law | ||
| D2 | Grant after examination | ||
| 8363 | Opposition against the patent | ||
| 8331 | Complete revocation |