DE3715033A1 - Verfahren zur isolierung von fusionsproteinen - Google Patents

Verfahren zur isolierung von fusionsproteinen

Info

Publication number
DE3715033A1
DE3715033A1 DE19873715033 DE3715033A DE3715033A1 DE 3715033 A1 DE3715033 A1 DE 3715033A1 DE 19873715033 DE19873715033 DE 19873715033 DE 3715033 A DE3715033 A DE 3715033A DE 3715033 A1 DE3715033 A1 DE 3715033A1
Authority
DE
Germany
Prior art keywords
fusion protein
solution
dialysis
urea
gene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
DE19873715033
Other languages
German (de)
English (en)
Inventor
Andreas Dr Plueckthun
Rudolf Dipl Chem Glockenhuber
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hoechst AG
Original Assignee
Hoechst AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoechst AG filed Critical Hoechst AG
Priority to DE19873715033 priority Critical patent/DE3715033A1/de
Priority to IL86276A priority patent/IL86276A0/xx
Priority to NZ224484A priority patent/NZ224484A/xx
Priority to FI882079A priority patent/FI882079A/fi
Priority to EP88107151A priority patent/EP0290005A2/de
Priority to NO881963A priority patent/NO881963L/no
Priority to ZA883208A priority patent/ZA883208B/xx
Priority to PT87414A priority patent/PT87414B/pt
Priority to AU15631/88A priority patent/AU1563188A/en
Priority to DK244988A priority patent/DK244988A/da
Priority to KR1019880005243A priority patent/KR880013979A/ko
Priority to JP63110294A priority patent/JPS6447394A/ja
Publication of DE3715033A1 publication Critical patent/DE3715033A1/de
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DE19873715033 1987-05-06 1987-05-06 Verfahren zur isolierung von fusionsproteinen Withdrawn DE3715033A1 (de)

Priority Applications (12)

Application Number Priority Date Filing Date Title
DE19873715033 DE3715033A1 (de) 1987-05-06 1987-05-06 Verfahren zur isolierung von fusionsproteinen
IL86276A IL86276A0 (en) 1987-05-06 1988-05-04 Process for the preparation of a genetically encodable polypeptide
NZ224484A NZ224484A (en) 1987-05-06 1988-05-04 Preparation of a fused polypeptide using beta-galactosidase to form an insoluble fusion protein
FI882079A FI882079A (fi) 1987-05-06 1988-05-04 Foerfarande foer framstaellning av en genetiskt kodbar polypeptid.
EP88107151A EP0290005A2 (de) 1987-05-06 1988-05-04 Verfahren zur Herstellung eines genetisch codierbaren Polypeptids
NO881963A NO881963L (no) 1987-05-06 1988-05-05 Fremgangsmaate for fremstilling av genetisk kodbare polypeptider.
ZA883208A ZA883208B (en) 1987-05-06 1988-05-05 A process for the preparation of a genetically encodable polypeptide
PT87414A PT87414B (pt) 1987-05-06 1988-05-05 Processo para a preparacao de um polipeptido geneticamente codificavel
AU15631/88A AU1563188A (en) 1987-05-06 1988-05-05 A process for the preparation of a genetically encodable polypeptide
DK244988A DK244988A (da) 1987-05-06 1988-05-05 Fremgangsmaade til fremstilling af et polypeptid, der kan kodes for genetisk
KR1019880005243A KR880013979A (ko) 1987-05-06 1988-05-06 유전적으로 암호화 가능한 폴리펩타이드의 제조방법
JP63110294A JPS6447394A (en) 1987-05-06 1988-05-06 Production of polypeptide capable of being encoded genetically

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19873715033 DE3715033A1 (de) 1987-05-06 1987-05-06 Verfahren zur isolierung von fusionsproteinen

Publications (1)

Publication Number Publication Date
DE3715033A1 true DE3715033A1 (de) 1988-11-17

Family

ID=6326923

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19873715033 Withdrawn DE3715033A1 (de) 1987-05-06 1987-05-06 Verfahren zur isolierung von fusionsproteinen

Country Status (12)

Country Link
EP (1) EP0290005A2 (no)
JP (1) JPS6447394A (no)
KR (1) KR880013979A (no)
AU (1) AU1563188A (no)
DE (1) DE3715033A1 (no)
DK (1) DK244988A (no)
FI (1) FI882079A (no)
IL (1) IL86276A0 (no)
NO (1) NO881963L (no)
NZ (1) NZ224484A (no)
PT (1) PT87414B (no)
ZA (1) ZA883208B (no)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3805150A1 (de) * 1987-04-11 1988-10-20 Hoechst Ag Gentechnologisches verfahren zur herstellung von polypeptiden
US5426036A (en) * 1987-05-05 1995-06-20 Hoechst Aktiengesellschaft Processes for the preparation of foreign proteins in streptomycetes
DE4012818A1 (de) 1990-04-21 1991-10-24 Hoechst Ag Verfahren zur herstellung von fremdproteinen in streptomyceten
ATE120761T1 (de) * 1987-05-21 1995-04-15 Creative Biomolecules Inc Multifunktionelle proteine mit vorbestimmter zielsetzung.
CA1339654C (en) * 1988-06-03 1998-02-03 Ursula A. Germann Gene therapy using gene fusion for genetic or acquired disorders
US5358857A (en) * 1989-08-29 1994-10-25 The General Hospital Corp. Method of preparing fusion proteins
US5227293A (en) * 1989-08-29 1993-07-13 The General Hospital Corporation Fusion proteins, their preparation and use

Also Published As

Publication number Publication date
FI882079A0 (fi) 1988-05-04
AU1563188A (en) 1988-11-10
KR880013979A (ko) 1988-12-22
PT87414B (pt) 1992-08-31
NO881963L (no) 1988-11-07
DK244988D0 (da) 1988-05-05
JPS6447394A (en) 1989-02-21
EP0290005A2 (de) 1988-11-09
DK244988A (da) 1988-11-07
NZ224484A (en) 1991-03-26
NO881963D0 (no) 1988-05-05
IL86276A0 (en) 1988-11-15
ZA883208B (en) 1988-11-08
FI882079A (fi) 1988-11-07
PT87414A (pt) 1989-05-31

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