DE3709959A1 - Verfahren und vorrichtung zur herstellung von enzymen mit einem rekombinante plasmide enthaltenden bakterium - Google Patents
Verfahren und vorrichtung zur herstellung von enzymen mit einem rekombinante plasmide enthaltenden bakteriumInfo
- Publication number
- DE3709959A1 DE3709959A1 DE19873709959 DE3709959A DE3709959A1 DE 3709959 A1 DE3709959 A1 DE 3709959A1 DE 19873709959 DE19873709959 DE 19873709959 DE 3709959 A DE3709959 A DE 3709959A DE 3709959 A1 DE3709959 A1 DE 3709959A1
- Authority
- DE
- Germany
- Prior art keywords
- repressor
- filter
- enzyme
- culture medium
- fermenter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000894006 Bacteria Species 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 13
- 108090000790 Enzymes Proteins 0.000 claims abstract description 38
- 102000004190 Enzymes Human genes 0.000 claims abstract description 31
- 102000008169 Co-Repressor Proteins Human genes 0.000 claims abstract description 26
- 108010060434 Co-Repressor Proteins Proteins 0.000 claims abstract description 26
- 239000001963 growth medium Substances 0.000 claims abstract description 24
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 15
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 6
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims description 17
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims description 17
- 239000000706 filtrate Substances 0.000 claims description 17
- 239000013612 plasmid Substances 0.000 claims description 13
- 241000588724 Escherichia coli Species 0.000 claims description 12
- 239000011148 porous material Substances 0.000 claims description 11
- 239000000919 ceramic Substances 0.000 claims description 8
- 108010005774 beta-Galactosidase Proteins 0.000 claims description 7
- 238000009295 crossflow filtration Methods 0.000 claims description 5
- 230000035897 transcription Effects 0.000 claims description 5
- 238000013518 transcription Methods 0.000 claims description 5
- 238000011001 backwashing Methods 0.000 claims description 4
- TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 claims description 4
- 239000002131 composite material Substances 0.000 claims description 2
- 239000012634 fragment Substances 0.000 claims description 2
- 229910021645 metal ion Inorganic materials 0.000 claims description 2
- 238000005215 recombination Methods 0.000 claims description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 229910010293 ceramic material Inorganic materials 0.000 claims 1
- 238000012258 culturing Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 description 14
- 238000001914 filtration Methods 0.000 description 12
- 230000000694 effects Effects 0.000 description 7
- 102000005936 beta-Galactosidase Human genes 0.000 description 5
- 230000007423 decrease Effects 0.000 description 5
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- WBQJTPDOGLYTBE-VIFPVBQESA-N 1-nitroso-L-tryptophan Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CN(N=O)C2=C1 WBQJTPDOGLYTBE-VIFPVBQESA-N 0.000 description 1
- 101100165177 Caenorhabditis elegans bath-15 gene Proteins 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 101150044453 Y gene Proteins 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000012365 batch cultivation Methods 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000011224 oxide ceramic Substances 0.000 description 1
- 229910052574 oxide ceramic Inorganic materials 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 229920003002 synthetic resin Polymers 0.000 description 1
- 239000000057 synthetic resin Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/14—Bioreactors or fermenters specially adapted for specific uses for producing enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
- C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
- C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Sustainable Development (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19873744928 DE3744928C2 (de) | 1986-03-26 | 1987-03-26 | Verfahren zur Herstellung eines rekombinanten Plasmids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP61067907A JPH0775536B2 (ja) | 1986-03-26 | 1986-03-26 | 遺伝子組み換え菌を用いた酵素生産装置 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3709959A1 true DE3709959A1 (de) | 1987-10-08 |
| DE3709959C2 DE3709959C2 (enrdf_load_stackoverflow) | 1991-04-25 |
Family
ID=13358439
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19873709959 Granted DE3709959A1 (de) | 1986-03-26 | 1987-03-26 | Verfahren und vorrichtung zur herstellung von enzymen mit einem rekombinante plasmide enthaltenden bakterium |
Country Status (4)
| Country | Link |
|---|---|
| JP (1) | JPH0775536B2 (enrdf_load_stackoverflow) |
| AU (1) | AU600710B2 (enrdf_load_stackoverflow) |
| DE (1) | DE3709959A1 (enrdf_load_stackoverflow) |
| GB (2) | GB2188324B (enrdf_load_stackoverflow) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01148318A (ja) * | 1987-12-04 | 1989-06-09 | Toshiba Ceramics Co Ltd | 固液分離装置 |
| NO305845B1 (no) | 1997-12-09 | 1999-08-02 | Kongsberg Offshore As | Forankringsmekanisme for en f°ringsstolpe |
| JP6321661B2 (ja) * | 2012-09-27 | 2018-05-09 | ジーイー・ヘルスケア・バイオサイエンス・アクチボラグ | タンジェンシャルフロー灌流システム及び方法 |
| CN106497765B (zh) * | 2016-11-24 | 2019-02-19 | 涡阳县华夏气体有限公司 | 一种工业酶制剂的精滤设备 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ZA811368B (en) * | 1980-03-24 | 1982-04-28 | Genentech Inc | Bacterial polypedtide expression employing tryptophan promoter-operator |
| JPS58141796A (ja) * | 1982-02-18 | 1983-08-23 | Kyowa Hakko Kogyo Co Ltd | ペプチドの製造法 |
| CA1338664C (en) * | 1985-04-10 | 1996-10-22 | Cetus Oncology Corporation | Recombinant hosts |
-
1986
- 1986-03-26 JP JP61067907A patent/JPH0775536B2/ja not_active Expired - Lifetime
-
1987
- 1987-03-25 GB GB8707130A patent/GB2188324B/en not_active Expired
- 1987-03-26 DE DE19873709959 patent/DE3709959A1/de active Granted
- 1987-03-26 AU AU70659/87A patent/AU600710B2/en not_active Ceased
-
1989
- 1989-12-22 GB GB8929030A patent/GB2225789B/en not_active Expired
Non-Patent Citations (1)
| Title |
|---|
| NICHTS ERMITTELT * |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62224286A (ja) | 1987-10-02 |
| GB2188324B (en) | 1990-12-05 |
| GB8929030D0 (en) | 1990-02-28 |
| GB2225789A (en) | 1990-06-13 |
| AU7065987A (en) | 1987-10-01 |
| AU600710B2 (en) | 1990-08-23 |
| GB2225789B (en) | 1990-09-12 |
| GB2188324A (en) | 1987-09-30 |
| GB8707130D0 (en) | 1987-04-29 |
| JPH0775536B2 (ja) | 1995-08-16 |
| DE3709959C2 (enrdf_load_stackoverflow) | 1991-04-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8110 | Request for examination paragraph 44 | ||
| 8172 | Supplementary division/partition in: |
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| Q171 | Divided out to: |
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| AH | Division in |
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| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| AH | Division in |
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| 8339 | Ceased/non-payment of the annual fee |