GB2188324B - Process for enzyme production with bacterium harboring recombinant plasmids. - Google Patents

Process for enzyme production with bacterium harboring recombinant plasmids.

Info

Publication number
GB2188324B
GB2188324B GB8707130A GB8707130A GB2188324B GB 2188324 B GB2188324 B GB 2188324B GB 8707130 A GB8707130 A GB 8707130A GB 8707130 A GB8707130 A GB 8707130A GB 2188324 B GB2188324 B GB 2188324B
Authority
GB
United Kingdom
Prior art keywords
recombinant plasmids
enzyme production
harboring recombinant
bacterium harboring
bacterium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB8707130A
Other versions
GB2188324A (en
GB8707130D0 (en
Inventor
Takeshi Kobayashi
Shinji Iijima
Masayuki Taniguchi
Shunji Yasuda
Yoshihisa Kato
Takashi Ogawa
Syuji Kawai
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Coorstek KK
Kao Corp
Original Assignee
Kao Corp
Toshiba Ceramics Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kao Corp, Toshiba Ceramics Co Ltd filed Critical Kao Corp
Publication of GB8707130D0 publication Critical patent/GB8707130D0/en
Publication of GB2188324A publication Critical patent/GB2188324A/en
Application granted granted Critical
Publication of GB2188324B publication Critical patent/GB2188324B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/14—Bioreactors or fermenters specially adapted for specific uses for producing enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/71—Expression systems using regulatory sequences derived from the trp-operon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
    • C12N9/2471—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
GB8707130A 1986-03-26 1987-03-25 Process for enzyme production with bacterium harboring recombinant plasmids. Expired - Fee Related GB2188324B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP61067907A JPH0775536B2 (en) 1986-03-26 1986-03-26 Enzyme production equipment using genetically modified bacteria

Publications (3)

Publication Number Publication Date
GB8707130D0 GB8707130D0 (en) 1987-04-29
GB2188324A GB2188324A (en) 1987-09-30
GB2188324B true GB2188324B (en) 1990-12-05

Family

ID=13358439

Family Applications (2)

Application Number Title Priority Date Filing Date
GB8707130A Expired - Fee Related GB2188324B (en) 1986-03-26 1987-03-25 Process for enzyme production with bacterium harboring recombinant plasmids.
GB8929030A Expired - Fee Related GB2225789B (en) 1986-03-26 1989-12-22 Culture apparatus for enzyme production

Family Applications After (1)

Application Number Title Priority Date Filing Date
GB8929030A Expired - Fee Related GB2225789B (en) 1986-03-26 1989-12-22 Culture apparatus for enzyme production

Country Status (4)

Country Link
JP (1) JPH0775536B2 (en)
AU (1) AU600710B2 (en)
DE (1) DE3709959A1 (en)
GB (2) GB2188324B (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01148318A (en) * 1987-12-04 1989-06-09 Toshiba Ceramics Co Ltd Solid-liquid separation device
NO305845B1 (en) 1997-12-09 1999-08-02 Kongsberg Offshore As Anchoring mechanism for a guide post
EP2900804B1 (en) * 2012-09-27 2019-09-18 GE Healthcare Bio-Sciences AB Tangential flow perfusion system
CN106497765B (en) * 2016-11-24 2019-02-19 涡阳县华夏气体有限公司 A kind of refined filtration equipment of industrial enzyme preparation

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2073203A (en) * 1980-03-24 1981-10-14 Genentech Inc Method of producing a polypeptide product and a plasmidic expression vehicle therefor,a method of creating an expression plasmid,a method of cleaving double stranded dna and specific plasmids
EP0197797A2 (en) * 1985-04-10 1986-10-15 Cetus Oncology Corporation Improved recombinant hosts, their production and use

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58141796A (en) * 1982-02-18 1983-08-23 Kyowa Hakko Kogyo Co Ltd Preparation of peptide

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2073203A (en) * 1980-03-24 1981-10-14 Genentech Inc Method of producing a polypeptide product and a plasmidic expression vehicle therefor,a method of creating an expression plasmid,a method of cleaving double stranded dna and specific plasmids
EP0154133A2 (en) * 1980-03-24 1985-09-11 Genentech, Inc. Plasmidic expression vehicles, and method of producing a polypeptide therewith
EP0197797A2 (en) * 1985-04-10 1986-10-15 Cetus Oncology Corporation Improved recombinant hosts, their production and use

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Molecular Cloning: A Laboratory Manu *

Also Published As

Publication number Publication date
DE3709959C2 (en) 1991-04-25
AU600710B2 (en) 1990-08-23
JPH0775536B2 (en) 1995-08-16
GB2188324A (en) 1987-09-30
JPS62224286A (en) 1987-10-02
DE3709959A1 (en) 1987-10-08
GB8929030D0 (en) 1990-02-28
GB2225789A (en) 1990-06-13
GB2225789B (en) 1990-09-12
GB8707130D0 (en) 1987-04-29
AU7065987A (en) 1987-10-01

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Legal Events

Date Code Title Description
PCNP Patent ceased through non-payment of renewal fee

Effective date: 19990325