DE3606231A1 - Verfahren zum messen des gesamtproteingehaltes einer probe mit hilfe eines elektrophoretischen bildes - Google Patents
Verfahren zum messen des gesamtproteingehaltes einer probe mit hilfe eines elektrophoretischen bildesInfo
- Publication number
- DE3606231A1 DE3606231A1 DE19863606231 DE3606231A DE3606231A1 DE 3606231 A1 DE3606231 A1 DE 3606231A1 DE 19863606231 DE19863606231 DE 19863606231 DE 3606231 A DE3606231 A DE 3606231A DE 3606231 A1 DE3606231 A1 DE 3606231A1
- Authority
- DE
- Germany
- Prior art keywords
- electrophoretic
- sample
- integrated
- image
- images
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 title claims description 28
- 235000018102 proteins Nutrition 0.000 claims description 54
- 102000004169 proteins and genes Human genes 0.000 claims description 54
- 108090000623 proteins and genes Proteins 0.000 claims description 54
- 238000012937 correction Methods 0.000 claims description 20
- 235000004252 protein component Nutrition 0.000 claims description 15
- 238000001962 electrophoresis Methods 0.000 claims description 13
- 238000010521 absorption reaction Methods 0.000 claims description 10
- 238000002835 absorbance Methods 0.000 claims description 8
- 230000002159 abnormal effect Effects 0.000 claims description 2
- 239000000969 carrier Substances 0.000 claims description 2
- 230000003287 optical effect Effects 0.000 claims description 2
- 230000005540 biological transmission Effects 0.000 claims 1
- 238000012360 testing method Methods 0.000 description 32
- 230000008033 biological extinction Effects 0.000 description 6
- 238000010186 staining Methods 0.000 description 5
- 102000009027 Albumins Human genes 0.000 description 4
- 108010088751 Albumins Proteins 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000011088 calibration curve Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 108010074605 gamma-Globulins Proteins 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 102000002572 Alpha-Globulins Human genes 0.000 description 2
- 108010068307 Alpha-Globulins Proteins 0.000 description 2
- 238000004040 coloring Methods 0.000 description 2
- NNBZCPXTIHJBJL-UHFFFAOYSA-N decalin Chemical compound C1CCCC2CCCCC21 NNBZCPXTIHJBJL-UHFFFAOYSA-N 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 108010044091 Globulins Proteins 0.000 description 1
- 102000006395 Globulins Human genes 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- CZBZUDVBLSSABA-UHFFFAOYSA-N butylated hydroxyanisole Chemical compound COC1=CC=C(O)C(C(C)(C)C)=C1.COC1=CC=C(O)C=C1C(C)(C)C CZBZUDVBLSSABA-UHFFFAOYSA-N 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000009499 grossing Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- PXXNTAGJWPJAGM-UHFFFAOYSA-N vertaline Natural products C1C2C=3C=C(OC)C(OC)=CC=3OC(C=C3)=CC=C3CCC(=O)OC1CC1N2CCCC1 PXXNTAGJWPJAGM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60036606A JPH065227B2 (ja) | 1985-02-27 | 1985-02-27 | 電気泳動装置による総蛋白値の定量方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3606231A1 true DE3606231A1 (de) | 1986-09-11 |
| DE3606231C2 DE3606231C2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1991-10-17 |
Family
ID=12474455
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19863606231 Granted DE3606231A1 (de) | 1985-02-27 | 1986-02-26 | Verfahren zum messen des gesamtproteingehaltes einer probe mit hilfe eines elektrophoretischen bildes |
Country Status (3)
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5627643A (en) * | 1992-04-07 | 1997-05-06 | Amersham Pharm Biotech Ab | Method and detector for separation processes |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8513538D0 (en) * | 1985-05-29 | 1985-07-03 | Mackay C D | Electrophoresis |
| US4920498A (en) * | 1985-08-17 | 1990-04-24 | Olympus Optical Co., Ltd. | Method of processing and analyzing electrophoretic image, and method of displaying electrophoregram and a medium for recording electrophoregram |
| JPH01155242A (ja) * | 1987-12-14 | 1989-06-19 | Shimadzu Corp | クロマトスキャナのデータ処理方法 |
| JP2762451B2 (ja) * | 1988-03-31 | 1998-06-04 | アイシン精機株式会社 | 遺伝子物質の電気泳動パターン分析装置 |
| US5844097A (en) * | 1990-11-30 | 1998-12-01 | Monoclonetics International, Inc. | Methods for the diagnosis of peripheral nerve damage |
| JPH06503418A (ja) * | 1990-11-30 | 1994-04-14 | モノクロネティックス インターナショナル、インコーポレーテッド | 慢性下位脊椎痛および頚部痛の診断法 |
| US5460709A (en) * | 1993-06-21 | 1995-10-24 | Helena Laboratories Corporation | Automatic electrophoresis method and apparatus |
| US5370347A (en) * | 1993-07-07 | 1994-12-06 | Helena Laboratories Corporation | Support system for an equipment housing |
| GB9509410D0 (en) | 1995-05-10 | 1995-07-05 | Imperial College | Molecular imaging |
| JP3091176B2 (ja) | 1998-02-18 | 2000-09-25 | 株式会社ヘレナ研究所 | 分離分析検査データ処理装置 |
| US6733645B1 (en) * | 2000-04-18 | 2004-05-11 | Caliper Technologies Corp. | Total analyte quantitation |
| AU2002212752A1 (en) | 2000-11-10 | 2002-05-21 | Arkray, Inc. | Measuring method and instrument comprising image sensor |
| DE10248555B4 (de) * | 2002-10-18 | 2004-12-02 | Roche Diagnostics Gmbh | Verfahren und Analysesystem zur Ermittlung der Konzentration eines Analyten in einer Probe, die aus dem Analyten und der Probenmatrix besteht und Testelement dafür |
| JP5575410B2 (ja) * | 2009-02-16 | 2014-08-20 | 株式会社東芝 | 自動分析装置 |
| CN101957337B (zh) * | 2010-08-26 | 2013-04-24 | 哈尔滨医科大学 | 一种电泳成分分析方法和系统 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE390766B (sv) * | 1972-12-19 | 1977-01-17 | Lkb Produkter Ab | Forfarande vid motflodesisotachofores |
| SE369279B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1973-01-15 | 1974-08-19 | Lkb Produkter Ab | |
| US3941678A (en) * | 1974-02-28 | 1976-03-02 | Shimadzu Seisakusho Ltd. | Apparatus for electrophoretic analysis |
| JPS5123795A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) * | 1974-08-21 | 1976-02-25 | Shimadzu Corp | |
| US4154669A (en) * | 1977-02-11 | 1979-05-15 | Pen Kem, Inc. | Automatic electrophoresis apparatus |
| US4181594A (en) * | 1979-03-27 | 1980-01-01 | University Of Pittsburgh | Matrix recovery electrophoresis apparatus |
| JPS55156851A (en) * | 1979-05-25 | 1980-12-06 | Olympus Optical Co Ltd | Fractionation processing method for electrophoresis |
| US4315812A (en) * | 1980-05-28 | 1982-02-16 | Karlson Eskil L | Apparatus for continuous electrochromatographic separation |
| US4420383A (en) * | 1980-07-10 | 1983-12-13 | Olympus Optical Co., Ltd. | Fractionating method in electrophoreses |
| DE3277081D1 (en) * | 1981-07-31 | 1987-10-01 | Shimadzu Corp | Electrophoretic apparatus |
| JPS5844340A (ja) * | 1981-09-10 | 1983-03-15 | Kureha Chem Ind Co Ltd | 電気泳動度測定装置 |
| US4594064A (en) * | 1983-11-10 | 1986-06-10 | The United States Of America As Represented By The United States Department Of Energy | Automated apparatus for producing gradient gels |
| US4569739A (en) * | 1984-12-31 | 1986-02-11 | Dorr-Oliver Incorporated | Electrofilter using an improved electrode assembly |
-
1985
- 1985-02-27 JP JP60036606A patent/JPH065227B2/ja not_active Expired - Lifetime
-
1986
- 1986-02-24 US US06/832,221 patent/US4666578A/en not_active Expired - Fee Related
- 1986-02-26 DE DE19863606231 patent/DE3606231A1/de active Granted
Non-Patent Citations (2)
| Title |
|---|
| MAURER, H.Rainer: Disk-Elektrophorese, Berlin, WAlter de Gruyter & Co., 1968, S. 78-85 * |
| Methoden der qualitativen und quantitativen Immunelektrophorese, Behring Institut (Behringwerke AG), Dezember 1975, S.10-15 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5627643A (en) * | 1992-04-07 | 1997-05-06 | Amersham Pharm Biotech Ab | Method and detector for separation processes |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH065227B2 (ja) | 1994-01-19 |
| JPS61196154A (ja) | 1986-08-30 |
| US4666578A (en) | 1987-05-19 |
| DE3606231C2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1991-10-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OP8 | Request for examination as to paragraph 44 patent law | ||
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |