DE3587883D1 - Synthetische Prosthetogene und ihre Verwendung in Enzymkopplungstests. - Google Patents

Synthetische Prosthetogene und ihre Verwendung in Enzymkopplungstests.

Info

Publication number
DE3587883D1
DE3587883D1 DE3587883T DE3587883T DE3587883D1 DE 3587883 D1 DE3587883 D1 DE 3587883D1 DE 3587883 T DE3587883 T DE 3587883T DE 3587883 T DE3587883 T DE 3587883T DE 3587883 D1 DE3587883 D1 DE 3587883D1
Authority
DE
Germany
Prior art keywords
enzyme
phosphate
prosthogenes
synthetic
catalyses
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE3587883T
Other languages
English (en)
Other versions
DE3587883T2 (de
Inventor
Robert Rabin
Christopher John Taylorson
Michael Robert C O An Hollaway
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
London Biotechnology Ltd
Original Assignee
London Biotechnology Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by London Biotechnology Ltd filed Critical London Biotechnology Ltd
Publication of DE3587883D1 publication Critical patent/DE3587883D1/de
Application granted granted Critical
Publication of DE3587883T2 publication Critical patent/DE3587883T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/966—Chemistry: molecular biology and microbiology involving an enzyme system with high turnover rate or complement magnified assay, e.g. multi-enzyme systems
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Saccharide Compounds (AREA)
DE3587883T 1984-03-26 1985-03-25 Synthetische Prosthetogene und ihre Verwendung in Enzymkopplungstests. Expired - Fee Related DE3587883T2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB848407736A GB8407736D0 (en) 1984-03-26 1984-03-26 Detecting specific polynucleotide sequences

Publications (2)

Publication Number Publication Date
DE3587883D1 true DE3587883D1 (de) 1994-08-18
DE3587883T2 DE3587883T2 (de) 1995-03-02

Family

ID=10558660

Family Applications (2)

Application Number Title Priority Date Filing Date
DE3587883T Expired - Fee Related DE3587883T2 (de) 1984-03-26 1985-03-25 Synthetische Prosthetogene und ihre Verwendung in Enzymkopplungstests.
DE8585302045T Expired - Lifetime DE3586183T2 (de) 1984-03-26 1985-03-25 Enzymatisches verfahren zur bestimmung von analyten und substrate hierzu.

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE8585302045T Expired - Lifetime DE3586183T2 (de) 1984-03-26 1985-03-25 Enzymatisches verfahren zur bestimmung von analyten und substrate hierzu.

Country Status (7)

Country Link
US (2) US4745054A (de)
EP (2) EP0156641B1 (de)
JP (1) JPS611398A (de)
AT (1) ATE108454T1 (de)
CA (1) CA1258673A (de)
DE (2) DE3587883T2 (de)
GB (3) GB8407736D0 (de)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8407736D0 (en) * 1984-03-26 1984-05-02 Univ London Detecting specific polynucleotide sequences
US5190864A (en) * 1986-04-15 1993-03-02 Northeastern University Enzyme amplification by using free enzyme to release enzyme from an immobilized enzyme material
US4937188A (en) * 1986-04-15 1990-06-26 Northeastern University Enzyme activity amplification method for increasing assay sensitivity
US4835099A (en) * 1986-11-20 1989-05-30 Becton, Dickinson And Company Signal enhancement in immunoassay by modulation of enzymatic catalysis
GB8628108D0 (en) * 1986-11-25 1986-12-31 London Biotechnology Ltd Riboflavin-linked assay
US5196306A (en) * 1989-03-29 1993-03-23 E. I. Du Pont De Nemours And Company Method for the detection or quantitation of an analyte using an analyte dependent enzyme activation system
ATE193019T1 (de) * 1989-10-13 2000-06-15 Zeneca Ltd Sonden
DE69024364T2 (de) * 1989-10-17 1996-07-11 British Tech Group Verstärkter chemilumineszenzassay
GB8927365D0 (en) * 1989-12-04 1990-01-31 Ici Plc Reagent
EP0597951B1 (de) * 1991-07-26 1999-03-31 Dade Chemistry Systems Inc. Signalerkennungspruefung in der anwesenheit von einem suspendierten festen träger
US5279937A (en) * 1992-04-30 1994-01-18 Detechnology Canada Use of macroglobulins to improve the signal-to-background ratio in affinity binding assays
US5985548A (en) * 1993-02-04 1999-11-16 E. I. Du Pont De Nemours And Company Amplification of assay reporters by nucleic acid replication
EP0716713A1 (de) * 1993-08-18 1996-06-19 Id Biomedical Corporation Zusammensetzungen und Verfahren zur Detektion von gezielten DNS Sequenzen durch Nützung von Molekülen mit Enzymen und damit angrenzenden Sequenzen
US5817455A (en) * 1994-03-01 1998-10-06 Novagen, Inc. Method for in vitro inactivation of RNase S
US5874216A (en) 1996-02-23 1999-02-23 Ensys Environmental Products, Inc. Indirect label assay device for detecting small molecules and method of use thereof
GB2347213B (en) * 1999-02-20 2001-06-27 Stuart Harbron Method for determining nuclease activity
US6322980B1 (en) * 1999-04-30 2001-11-27 Aclara Biosciences, Inc. Single nucleotide detection using degradation of a fluorescent sequence
US6514700B1 (en) * 1999-04-30 2003-02-04 Aclara Biosciences, Inc. Nucleic acid detection using degradation of a tagged sequence
US7537938B2 (en) * 2000-04-28 2009-05-26 Monogram Biosciences, Inc. Biomarker detection in circulating cells
US7771929B2 (en) * 2000-04-28 2010-08-10 Monogram Biosciences, Inc. Tag library compounds, compositions, kits and methods of use
US20030207300A1 (en) * 2000-04-28 2003-11-06 Matray Tracy J. Multiplex analytical platform using molecular tags
US7160735B2 (en) * 2000-04-28 2007-01-09 Monogram Biosciences, Inc. Tagged microparticle compositions and methods
WO2002094998A2 (en) 2001-05-21 2002-11-28 Aclara Biosciences, Inc. Analyzing phosphorylated proteins
PL373962A1 (en) * 2001-05-21 2005-09-19 Aclara Biosciences, Inc. Methods and compositions for analyzing proteins
WO2002097112A2 (en) * 2001-05-26 2002-12-05 Aclara Biosciences, Inc. Catalytic amplification of multiplexed assay signals
FR2827304B1 (fr) 2001-07-16 2004-05-21 Commissariat Energie Atomique Procede et support d'analyse biologique utilisant des oligonucleotides comportant un marqueur activable enzymatiquement
US7045311B2 (en) * 2001-10-25 2006-05-16 Monogram Biosciences, Inc. Whole cell assay systems for cell surface proteases
CN1166422C (zh) * 2001-11-05 2004-09-15 北京源德生物医学工程股份有限公司 用于体外高能聚焦超声波治疗机的坐位架
US20050053939A1 (en) * 2001-11-09 2005-03-10 Ahmed Chenna Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents
US20040229294A1 (en) * 2002-05-21 2004-11-18 Po-Ying Chan-Hui ErbB surface receptor complexes as biomarkers
US7402397B2 (en) * 2002-05-21 2008-07-22 Monogram Biosciences, Inc. Detecting and profiling molecular complexes
US20040229299A1 (en) * 2002-05-21 2004-11-18 Badal M. Youssouf Intracellular complexes as biomarkers
US20040175765A1 (en) * 2002-07-05 2004-09-09 Sharat Singh Cell-screening assay and composition
US20050003369A1 (en) * 2002-10-10 2005-01-06 Affymetrix, Inc. Method for depleting specific nucleic acids from a mixture
EP1680666A4 (de) * 2003-10-27 2008-03-26 Monogram Biosciences Inc Nachweisen von menschlichen antitherapeutischen antikörpern
US7790364B2 (en) * 2006-01-27 2010-09-07 The Board Of Trustees Of The Leland Stanford Junior University Compositions and methods for high throughput screening of pharmacological chaperones
JP2022108322A (ja) 2021-01-13 2022-07-26 株式会社日立ハイテク 分離カラム接続装置及び分離装置
WO2023091588A1 (en) * 2021-11-17 2023-05-25 Nerd Bio Llc Systems and methods for real-time cellular drug-target engagement

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US3852157A (en) * 1971-05-14 1974-12-03 Syva Corp Compounds for enzyme amplification assay
IL39323A (en) * 1971-05-14 1975-08-31 Syva Co Highly sensitive assay method employing an enzyme for amplification
WO1981000725A1 (en) * 1979-09-14 1981-03-19 Charles Hospital Dev Method of determining a substrate in a sample
GB2059421A (en) * 1979-10-03 1981-04-23 Self C H Assay method and reagents therefor
US4259232A (en) * 1979-10-23 1981-03-31 Miles Laboratories, Inc. Flavin adenine dinucleotide-labeled protein and polypeptide conjugates
US4378428A (en) * 1981-03-30 1983-03-29 Baker Instruments Corporation Method for carrying out non-isotopic immunoassays, labeled analytes and kits for use in such assays
US4463090A (en) * 1981-09-30 1984-07-31 Harris Curtis C Cascade amplification enzyme immunoassay
JPS59140900A (ja) * 1983-01-28 1984-08-13 Toyo Jozo Co Ltd 新規な酵素的高感度測定法
DK147184A (da) * 1983-04-18 1984-10-19 Du Pont Fremgangsmaade og reagens til kvantitativ immunokemisk analyse
JPS59213399A (ja) * 1983-05-18 1984-12-03 Toyo Jozo Co Ltd Ｎｈ↓３またはａｔｐの測定法
GB8407736D0 (en) * 1984-03-26 1984-05-02 Univ London Detecting specific polynucleotide sequences

Also Published As

Publication number Publication date
EP0461731A2 (de) 1991-12-18
GB2192889A (en) 1988-01-27
ATE108454T1 (de) 1994-07-15
GB8507686D0 (en) 1985-05-01
DE3586183D1 (de) 1992-07-16
EP0461731A3 (en) 1992-07-15
GB2192889B (en) 1988-06-08
CA1258673A (en) 1989-08-22
JPH0586199B2 (de) 1993-12-10
EP0156641A3 (en) 1987-12-02
GB8718586D0 (en) 1987-09-09
US5057412A (en) 1991-10-15
US4745054A (en) 1988-05-17
EP0461731B1 (de) 1994-07-13
EP0156641A2 (de) 1985-10-02
JPS611398A (ja) 1986-01-07
GB2156518B (en) 1988-06-08
DE3587883T2 (de) 1995-03-02
EP0156641B1 (de) 1992-06-10
DE3586183T2 (de) 1993-01-21
GB2156518A (en) 1985-10-09
GB8407736D0 (en) 1984-05-02

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee