GB2347213B - Method for determining nuclease activity - Google Patents

Method for determining nuclease activity

Info

Publication number
GB2347213B
GB2347213B GB9903851A GB9903851A GB2347213B GB 2347213 B GB2347213 B GB 2347213B GB 9903851 A GB9903851 A GB 9903851A GB 9903851 A GB9903851 A GB 9903851A GB 2347213 B GB2347213 B GB 2347213B
Authority
GB
United Kingdom
Prior art keywords
nuclease activity
determining
determining nuclease
activity
nuclease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
GB9903851A
Other versions
GB2347213A (en
GB9903851D0 (en
Inventor
Stuart Harbron
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zetatronics Ltd
Original Assignee
Zetatronics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zetatronics Ltd filed Critical Zetatronics Ltd
Priority to GB9903851A priority Critical patent/GB2347213B/en
Publication of GB9903851D0 publication Critical patent/GB9903851D0/en
Priority to PCT/GB2000/000606 priority patent/WO2000049172A1/en
Priority to AU25656/00A priority patent/AU2565600A/en
Priority to EP00903907A priority patent/EP1155143A1/en
Publication of GB2347213A publication Critical patent/GB2347213A/en
Application granted granted Critical
Publication of GB2347213B publication Critical patent/GB2347213B/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/573—Immunoassay; Biospecific binding assay; Materials therefor for enzymes or isoenzymes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/916—Hydrolases (3) acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
    • G01N2333/922—Ribonucleases (RNAses); Deoxyribonucleases (DNAses)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
GB9903851A 1999-02-20 1999-02-20 Method for determining nuclease activity Expired - Fee Related GB2347213B (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
GB9903851A GB2347213B (en) 1999-02-20 1999-02-20 Method for determining nuclease activity
PCT/GB2000/000606 WO2000049172A1 (en) 1999-02-20 2000-02-21 Determination of nuclease activity
AU25656/00A AU2565600A (en) 1999-02-20 2000-02-21 Determination of nuclease activity
EP00903907A EP1155143A1 (en) 1999-02-20 2000-02-21 Determination of nuclease activity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB9903851A GB2347213B (en) 1999-02-20 1999-02-20 Method for determining nuclease activity

Publications (3)

Publication Number Publication Date
GB9903851D0 GB9903851D0 (en) 1999-04-14
GB2347213A GB2347213A (en) 2000-08-30
GB2347213B true GB2347213B (en) 2001-06-27

Family

ID=10848124

Family Applications (1)

Application Number Title Priority Date Filing Date
GB9903851A Expired - Fee Related GB2347213B (en) 1999-02-20 1999-02-20 Method for determining nuclease activity

Country Status (4)

Country Link
EP (1) EP1155143A1 (en)
AU (1) AU2565600A (en)
GB (1) GB2347213B (en)
WO (1) WO2000049172A1 (en)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2156518A (en) * 1984-03-26 1985-10-09 Univ London Enzymic method of detecting analytes and novel substrates therefor
WO1998019168A1 (en) * 1996-10-29 1998-05-07 London Biotechnology Limited Assays and probes with enzyme labels
GB2324370A (en) * 1997-04-14 1998-10-21 Stuart Harbron Method for detecting nucleic acids

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU8043982A (en) * 1981-02-12 1982-08-19 Self, C.H. Method of detecting phosphatase by contacting with nicotinamide coenzyme
US4931223A (en) * 1986-07-24 1990-06-05 Tropix, Inc. Methods of using chemiluminescent 1,2-dioxetanes

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2156518A (en) * 1984-03-26 1985-10-09 Univ London Enzymic method of detecting analytes and novel substrates therefor
WO1998019168A1 (en) * 1996-10-29 1998-05-07 London Biotechnology Limited Assays and probes with enzyme labels
GB2324370A (en) * 1997-04-14 1998-10-21 Stuart Harbron Method for detecting nucleic acids

Also Published As

Publication number Publication date
EP1155143A1 (en) 2001-11-21
WO2000049172A1 (en) 2000-08-24
AU2565600A (en) 2000-09-04
GB2347213A (en) 2000-08-30
GB9903851D0 (en) 1999-04-14

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Legal Events

Date Code Title Description
COOA Change in applicant's name or ownership of the application
PCNP Patent ceased through non-payment of renewal fee

Effective date: 20060220