DE3584482D1 - Verfahren zur herstellung elektrophoren gelmaterials. - Google Patents

Verfahren zur herstellung elektrophoren gelmaterials.

Info

Publication number
DE3584482D1
DE3584482D1 DE8585107899T DE3584482T DE3584482D1 DE 3584482 D1 DE3584482 D1 DE 3584482D1 DE 8585107899 T DE8585107899 T DE 8585107899T DE 3584482 T DE3584482 T DE 3584482T DE 3584482 D1 DE3584482 D1 DE 3584482D1
Authority
DE
Germany
Prior art keywords
electrophoric
producing
gel material
gel
producing electrophoric
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE8585107899T
Other languages
English (en)
Inventor
Baylor, Jr
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Application granted granted Critical
Publication of DE3584482D1 publication Critical patent/DE3584482D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F20/00—Homopolymers and copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride, ester, amide, imide or nitrile thereof
    • C08F20/02—Monocarboxylic acids having less than ten carbon atoms, Derivatives thereof
    • C08F20/52—Amides or imides
    • C08F20/54—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide
    • C08F20/56—Acrylamide; Methacrylamide
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S522/00—Synthetic resins or natural rubbers -- part of the class 520 series
    • Y10S522/91—Treatment through an external filter or mask, nonphotograghic process

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Electrochemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Organic Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Polymerisation Methods In General (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Medicinal Preparation (AREA)
DE8585107899T 1984-06-28 1985-06-26 Verfahren zur herstellung elektrophoren gelmaterials. Expired - Lifetime DE3584482D1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/625,840 US4790919A (en) 1984-06-28 1984-06-28 Process for preparation of electrophoresis gel material

Publications (1)

Publication Number Publication Date
DE3584482D1 true DE3584482D1 (de) 1991-11-28

Family

ID=24507819

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8585107899T Expired - Lifetime DE3584482D1 (de) 1984-06-28 1985-06-26 Verfahren zur herstellung elektrophoren gelmaterials.

Country Status (8)

Country Link
US (1) US4790919A (de)
EP (1) EP0169397B1 (de)
JP (1) JPH0625748B2 (de)
CA (1) CA1261784A (de)
DE (1) DE3584482D1 (de)
DK (1) DK167396B1 (de)
GR (1) GR851565B (de)
IE (1) IE58481B1 (de)

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US4909977A (en) * 1987-05-20 1990-03-20 Bios Corporation Method for molding thin gel slabs horizontally with integrally molded large volume sample wells
US4968535A (en) * 1987-05-29 1990-11-06 Fuji Photo Film Co., Ltd. Method of producing gradient gel medium membrane for electrophoresis
JPS63298046A (ja) * 1987-05-29 1988-12-05 Fuji Photo Film Co Ltd 電気泳動用グラジェントゲル膜の製造方法
US5190629A (en) * 1987-07-16 1993-03-02 Fuji Photo Film Co., Ltd. Electrophoresis medium membrane
US5219923A (en) * 1988-04-29 1993-06-15 At Biochem, Inc. Electrophoretic media
GB8829802D0 (en) * 1988-12-21 1989-02-15 Prittie Allan R Improved raised image plate construction
US5061336A (en) * 1989-05-01 1991-10-29 Soane Technologies, Inc. Gel casting method and apparatus
AU644553B2 (en) * 1990-03-20 1993-12-09 At Biochem, Inc. Electrophoretic media
JP3065099B2 (ja) * 1990-03-20 2000-07-12 エイティー バイオケム インコーポレイテッド 電気泳動用媒質
US5200150A (en) * 1990-06-14 1993-04-06 Hewlett-Packard Company Preparation of gel-filled separation columns
US5061355A (en) * 1990-06-14 1991-10-29 Hewlett-Packard Company Preparation of gel-filled separation columns
US5634943A (en) * 1990-07-12 1997-06-03 University Of Miami Injectable polyethylene oxide gel implant and method for production
JP2588059B2 (ja) * 1990-11-19 1997-03-05 ハイモ株式会社 電気泳動用ポリアクリルアミドゲルの製造方法
GB9115073D0 (en) * 1991-07-12 1991-08-28 Astromed Ltd Improvements in electrophoretic separation
US5321357A (en) * 1992-08-07 1994-06-14 Yale University Three-dimensional detection, dosimetry and imaging of an energy field by formation of a polymer in a gel
US5637202A (en) * 1993-10-27 1997-06-10 California Institute Of Technology Porous electrophoresis sponges
AU1183195A (en) * 1993-11-17 1995-06-06 Applied Hydrogel Technology Corporation Methods for the separation of biological materials
US6783651B1 (en) * 1994-03-31 2004-08-31 Invitrogen Corporation System for pH-neutral stable electrophoresis gel
US5507934A (en) * 1994-11-01 1996-04-16 Visible Genetics Inc. Apparatus for preparing gels for use in electrophoretic separations and similar applications
US5630924A (en) 1995-04-20 1997-05-20 Perseptive Biosystems, Inc. Compositions, methods and apparatus for ultrafast electroseparation analysis
US5916427A (en) * 1995-11-08 1999-06-29 Kirkpatrick; Francis H Methods and reagents for gel electrophoresis
US5618398A (en) * 1995-12-12 1997-04-08 Visible Genetics Inc. Electrophoresis gels and gel holders having fiber spacers and method of making same
US5599434A (en) * 1995-12-12 1997-02-04 Visible Genetics Inc. Electrophoresis gels and gel holders having adhesive affixed fiber spacers and method of making same
SE9604730D0 (sv) * 1996-12-20 1996-12-20 Pharmacia Biotech Ab Electrophoretic gel
US5975895A (en) * 1997-11-12 1999-11-02 Coltene/Whaledent Strobe light curing apparatus and method
US6187250B1 (en) * 1998-08-19 2001-02-13 James Champagne Continuous gel casting method and apparatus
AU2025400A (en) * 1998-11-17 2000-06-05 Proteo Tools Separation, screening, and identification of biological targets
EP1983331B1 (de) * 1999-05-28 2011-07-13 Yokogawa Electric Corporation Optische Anordnung zur Auswertung eines Biochips
EP1126275A3 (de) * 2000-02-18 2002-12-18 The Board Of Trustees Of The Leland Stanford Junior University Kapillarsäule aus geschmolzenem Siliziumdioxid mit Photopolymerbestandteilen
US6875348B2 (en) * 2000-02-18 2005-04-05 The Board Of Trustees Of The Leland Stanford Junior University Separation column having a photopolymerized sol-gel component and associated methods
EP1126276A3 (de) * 2000-02-18 2002-12-18 The Board of Trustees of The Leland S. Stanford Junior University Trennungssäule, die eine poröse Photopolymer-Matrix enthält, sowie Füllungsverfahren der Säule
US6866785B2 (en) * 2001-08-13 2005-03-15 The Board Of Trustees Of The Leland Stanford Junior University Photopolymerized sol-gel column and associated methods
AU2001274887A1 (en) * 2000-05-23 2001-12-03 Mj Geneworks, Inc. Electrophoretic stacking of dna and other samples prior to electrophoresis
GB0104142D0 (en) * 2001-02-20 2001-04-11 Ciba Spec Chem Water Treat Ltd Polymerisation process
JP2004250673A (ja) * 2003-01-28 2004-09-09 National Institute For Materials Science ゲル化コロイド結晶前駆体とゲル化コロイド結晶、及びゲル化コロイド結晶の作製方法とその作製装置
US7622028B2 (en) 2003-05-09 2009-11-24 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
US20060194306A1 (en) * 2003-08-25 2006-08-31 Herr Amy E System for gel electrophoretic immunoassay
US20060191792A1 (en) * 2003-08-25 2006-08-31 Herr Amy E Method and apparatus for gel electrophoretic immunoassay
US20050127002A1 (en) * 2003-12-12 2005-06-16 Zare Richard N. Immobilized-enzyme microreactor devices for characterization of biomolecular analytes and associated methods
US20070284308A1 (en) * 2006-06-12 2007-12-13 Zare Richard N Immobilized-enzyme microreactor devices for characterization of biomolecular analytes and associated methods
CN101616936B (zh) * 2007-02-23 2012-08-08 巴斯夫欧洲公司 高吸水性聚合物在连续带式反应器上的生产
JP2009156767A (ja) * 2007-12-27 2009-07-16 National Institute Of Advanced Industrial & Technology タンパク質アレイ基板
US9481778B2 (en) 2009-09-09 2016-11-01 Northeastern University Initiator-tightened compositions
US9151732B2 (en) * 2010-10-01 2015-10-06 The Board Of Trustees Of The Leland Stanford Junior University Enhanced isotachophoresis assays using additives with spatial gradients
US9976984B2 (en) * 2013-05-10 2018-05-22 The Regents Of The University Of California Free-standing microfluidic gel electrophoresis devices and methods
JP6605454B2 (ja) * 2013-09-24 2019-11-13 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア グレースケールマスクを用いた分離媒体の生成
EP4134668A1 (de) 2015-05-20 2023-02-15 ProteinSimple System und verfahren zur elektrophoretischen trennung und analyse von analyten
US10761425B2 (en) * 2015-11-23 2020-09-01 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Particulate photoinitiators and uses thereof
EP3408389B1 (de) 2016-01-29 2021-03-10 Purigen Biosystems, Inc. Isotachophorese zur reinigung von nukleinsäuren
US11041150B2 (en) 2017-08-02 2021-06-22 Purigen Biosystems, Inc. Systems, devices, and methods for isotachophoresis
WO2023220530A1 (en) * 2022-05-10 2023-11-16 Emd Millipore Corporation Sample well comb mask for photopolymerized electrophoretic gels
WO2023248280A1 (ja) * 2022-06-20 2023-12-28 株式会社東陽テクニカ マイクロチップ、２次元試料分離システム及びマイクロチップの製造方法

Family Cites Families (18)

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CA584381A (en) * 1959-10-06 J. Van Oss Carel Separation of particles of different materials
US2875047A (en) * 1955-01-19 1959-02-24 Oster Gerald Photopolymerization with the formation of coherent plastic masses
GB1054982A (de) * 1963-04-22
US3549366A (en) * 1967-02-16 1970-12-22 Hughes Aircraft Co Ultraviolet hardening of photosensitized polyacrylamide and products
US3773209A (en) * 1971-12-30 1973-11-20 American Can Co Full or partial opening easy-open container end closure
US3847785A (en) * 1972-02-14 1974-11-12 Instr Specialties Co Inc Electrophoresis aparatus
US4248685A (en) * 1972-07-10 1981-02-03 Johnson & Johnson Method for making hydrophilic random interpolymer compositions
US4133942A (en) * 1972-11-06 1979-01-09 Ceskoslovenska Akademie Ved Polymeric carrier for a controlled synthesis of peptides and the method of its preparation
JPS5142084A (de) * 1974-10-08 1976-04-09 Akuriru Kogyo Kk
US4192727A (en) * 1976-08-24 1980-03-11 Union Carbide Corporation Polyelectrolyte hydrogels and methods of their preparation
US4204928A (en) * 1977-03-01 1980-05-27 Stauffer Chemical Company Photopolymerizable composition stabilized with nitrogen-containing aromatic compounds
DE2718700A1 (de) * 1977-04-27 1978-11-02 Hans A Dipl Chem Dr Thoma Verfahren zur gesamtbestimmung von hormonen und pharmaka
DE2722751C2 (de) * 1977-05-20 1990-06-21 Röhm GmbH, 6100 Darmstadt Perlpolymerisat mit einem Teilchendurchmesser von 5 bis 1000 μm und innerem Hohlraum
US4189370A (en) * 1978-06-21 1980-02-19 Societe Sebia Process for obtaining gels of N-methylol-acrylamide copolymers and application of said gels for the stepped gradient separation of seric lipoproteins
FR2453185A1 (fr) * 1979-04-05 1980-10-31 Rhone Poulenc Ind Procede de preparation de polymeres ou copolymeres acryliques hydrosolubles a poids moleculaire eleve et a faible teneur en monomere(s) residuels(s)
CA1206666A (en) * 1982-02-19 1986-06-24 Samuel Nochumson Copolymer of acrylamide and polysaccharide resin as electrophoretic gel medium
JPS59136648A (ja) * 1983-01-26 1984-08-06 Fuji Photo Film Co Ltd ゲル電気泳動用媒体
US4704198A (en) * 1984-04-27 1987-11-03 E. I. Du Pont De Nemours And Company Porosity gradient electrophoresis gel

Also Published As

Publication number Publication date
EP0169397B1 (de) 1991-10-23
IE851598L (en) 1985-12-28
DK167396B1 (da) 1993-10-25
US4790919A (en) 1988-12-13
CA1261784A (en) 1989-09-26
GR851565B (de) 1985-11-25
DK296385A (da) 1985-12-29
DK296385D0 (da) 1985-06-28
JPS6128512A (ja) 1986-02-08
IE58481B1 (en) 1993-09-22
EP0169397A1 (de) 1986-01-29
JPH0625748B2 (ja) 1994-04-06

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition