DE3537199A1 - Verfahren zum ermitteln des mittelpunktes eines elektrophoretischen bildes - Google Patents
Verfahren zum ermitteln des mittelpunktes eines elektrophoretischen bildesInfo
- Publication number
- DE3537199A1 DE3537199A1 DE19853537199 DE3537199A DE3537199A1 DE 3537199 A1 DE3537199 A1 DE 3537199A1 DE 19853537199 DE19853537199 DE 19853537199 DE 3537199 A DE3537199 A DE 3537199A DE 3537199 A1 DE3537199 A1 DE 3537199A1
- Authority
- DE
- Germany
- Prior art keywords
- point
- electrophoretic
- determined
- leading edge
- optical density
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000758 substrate Substances 0.000 claims abstract description 38
- 102000009027 Albumins Human genes 0.000 claims abstract description 15
- 108010088751 Albumins Proteins 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 15
- 210000002966 serum Anatomy 0.000 claims abstract description 4
- 230000003287 optical effect Effects 0.000 claims description 52
- 238000001962 electrophoresis Methods 0.000 claims description 8
- 102000006395 Globulins Human genes 0.000 abstract description 2
- 108010044091 Globulins Proteins 0.000 abstract description 2
- NNBZCPXTIHJBJL-UHFFFAOYSA-N decalin Chemical compound C1CCCC2CCCCC21 NNBZCPXTIHJBJL-UHFFFAOYSA-N 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000009826 distribution Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- PXXNTAGJWPJAGM-UHFFFAOYSA-N vertaline Natural products C1C2C=3C=C(OC)C(OC)=CC=3OC(C=C3)=CC=C3CCC(=O)OC1CC1N2CCCC1 PXXNTAGJWPJAGM-UHFFFAOYSA-N 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229940057995 liquid paraffin Drugs 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B11/00—Measuring arrangements characterised by the use of optical techniques
- G01B11/02—Measuring arrangements characterised by the use of optical techniques for measuring length, width or thickness
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP59221133A JPH0621864B2 (ja) | 1984-10-23 | 1984-10-23 | 電気泳動像の分析方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3537199A1 true DE3537199A1 (de) | 1986-04-24 |
| DE3537199C2 DE3537199C2 (enExample) | 1989-03-30 |
Family
ID=16761970
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19853537199 Granted DE3537199A1 (de) | 1984-10-23 | 1985-10-18 | Verfahren zum ermitteln des mittelpunktes eines elektrophoretischen bildes |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JPH0621864B2 (enExample) |
| DE (1) | DE3537199A1 (enExample) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS57204437A (en) * | 1981-06-11 | 1982-12-15 | Hiranuma Sangyo Kk | Measuring method for interval of inspection body in concentration measuring apparatus |
| JPS5845540A (ja) * | 1981-09-14 | 1983-03-16 | Toyo Kagaku Sangyo Kk | デンシトメ−タ−における検体の中心部検出方法 |
-
1984
- 1984-10-23 JP JP59221133A patent/JPH0621864B2/ja not_active Expired - Lifetime
-
1985
- 1985-10-18 DE DE19853537199 patent/DE3537199A1/de active Granted
Non-Patent Citations (1)
| Title |
|---|
| Touchstone, Joseph. C.: Advances in Thin Layer Chromatography, New York u.a. 1982, John Wiley & Sons, S.132-137 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6199841A (ja) | 1986-05-17 |
| DE3537199C2 (enExample) | 1989-03-30 |
| JPH0621864B2 (ja) | 1994-03-23 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OP8 | Request for examination as to paragraph 44 patent law | ||
| 8125 | Change of the main classification |
Ipc: G01N 27/26 |
|
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |