DE3329659A1 - Verfahren zur chemischen aenderung der substratspezifitaet eines nativen proteins zur erzeugung eines vorbestimmten modifizierten enzymartigen proteins - Google Patents

Verfahren zur chemischen aenderung der substratspezifitaet eines nativen proteins zur erzeugung eines vorbestimmten modifizierten enzymartigen proteins

Info

Publication number
DE3329659A1
DE3329659A1 DE19833329659 DE3329659A DE3329659A1 DE 3329659 A1 DE3329659 A1 DE 3329659A1 DE 19833329659 DE19833329659 DE 19833329659 DE 3329659 A DE3329659 A DE 3329659A DE 3329659 A1 DE3329659 A1 DE 3329659A1
Authority
DE
Germany
Prior art keywords
protein
inhibitor
column
enzyme
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
DE19833329659
Other languages
German (de)
English (en)
Other versions
DE3329659C2 (enExample
Inventor
Melvin Hubert 43560 Sylvania Ohio Keyes
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
OI Glass Inc
Original Assignee
Owens Illinois Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Owens Illinois Inc filed Critical Owens Illinois Inc
Publication of DE3329659A1 publication Critical patent/DE3329659A1/de
Application granted granted Critical
Publication of DE3329659C2 publication Critical patent/DE3329659C2/de
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
DE19833329659 1982-09-16 1983-08-17 Verfahren zur chemischen aenderung der substratspezifitaet eines nativen proteins zur erzeugung eines vorbestimmten modifizierten enzymartigen proteins Granted DE3329659A1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US41888482A 1982-09-16 1982-09-16

Publications (2)

Publication Number Publication Date
DE3329659A1 true DE3329659A1 (de) 1984-03-22
DE3329659C2 DE3329659C2 (enExample) 1987-08-13

Family

ID=23659947

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19833329659 Granted DE3329659A1 (de) 1982-09-16 1983-08-17 Verfahren zur chemischen aenderung der substratspezifitaet eines nativen proteins zur erzeugung eines vorbestimmten modifizierten enzymartigen proteins

Country Status (8)

Country Link
JP (1) JPS5966884A (enExample)
AU (1) AU539729B2 (enExample)
CA (1) CA1200518A (enExample)
DE (1) DE3329659A1 (enExample)
FR (1) FR2533217B1 (enExample)
GB (1) GB2127025B (enExample)
IT (1) IT1171852B (enExample)
MX (1) MX7645E (enExample)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4001085A (en) * 1973-09-10 1977-01-04 Owens-Illinois, Inc. Immobilization of enzymes on an inorganic matrix

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5325030B2 (enExample) * 1972-12-19 1978-07-24
US4716116A (en) * 1980-12-04 1987-12-29 Owens-Illinois Glass Container Inc. Protein modification to provide enzyme activity

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4001085A (en) * 1973-09-10 1977-01-04 Owens-Illinois, Inc. Immobilization of enzymes on an inorganic matrix

Non-Patent Citations (21)

* Cited by examiner, † Cited by third party
Title
Advances in Chemistry Series, 1971, 21-43 *
Advances in Protein Chemistry, Vol. 24, 2-97, Vol. 33, 74-166, 167-192 *
Analytical Biochemistry, 82, 1977, 83-100 *
Augen.Chem.Int.Ed.Engl., 10, 795 *
BBA, Vol. 621, 227-232 *
BERSIN: Kurzes Lehrbuch der Enzymologie, 1951, 42 *
Biochemistry, 58,1970 *
Biomimethic Chemistry, 35, 1980 *
Bioorganic Chemistry, 6, 1977, 165 *
Handbook of Enzyme Inhibitors, Inhibitor Index, 1-380, 1982 *
Int.J. Peptide Res., 5, 1973, 49-62, 215-218 *
Israel J.Chem., 17, 291, 1978 *
J.Amer.Chem.Soc., 100, 306, 3227, 3918, 7670, 1978 *
J.C.S.Chem.Comm., 11, 457, 1978 *
Journal of Biological Chemistry, 248, 10, 1973, 3750-3752 *
KIRK-OTHMER: Encyclopedia of Chemical Technology, 9, 148-172, 1980 *
Methods Enzymol., 11, 1967, 617 *
Molekulyarnaya Biologiya, Vol. 8, Nr. 6, 857-863 *
Proceedings of a Meeting held inBasel, 20. u. 21.03.80, Inhibitor Index, 275-278 *
SemisyntheticProteins, 1980 *
TIBS, Dez. 1979, 271 *

Also Published As

Publication number Publication date
GB2127025A (en) 1984-04-04
DE3329659C2 (enExample) 1987-08-13
IT8348717A1 (it) 1985-01-20
JPS5966884A (ja) 1984-04-16
FR2533217B1 (fr) 1987-01-30
CA1200518A (en) 1986-02-11
AU539729B2 (en) 1984-10-11
AU1450783A (en) 1984-05-17
JPS613478B2 (enExample) 1986-02-01
GB8314852D0 (en) 1983-07-06
IT8348717A0 (it) 1983-07-20
FR2533217A1 (fr) 1984-03-23
MX7645E (es) 1990-05-30
GB2127025B (en) 1986-01-15
IT1171852B (it) 1987-06-10

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Legal Events

Date Code Title Description
OP8 Request for examination as to paragraph 44 patent law
D2 Grant after examination
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee