DE3323949A1 - Verfahren und mittel zur raschen und vollstaendigen beseitigung einer truebung in einer biologischen fluessigkeit - Google Patents
Verfahren und mittel zur raschen und vollstaendigen beseitigung einer truebung in einer biologischen fluessigkeitInfo
- Publication number
- DE3323949A1 DE3323949A1 DE19833323949 DE3323949A DE3323949A1 DE 3323949 A1 DE3323949 A1 DE 3323949A1 DE 19833323949 DE19833323949 DE 19833323949 DE 3323949 A DE3323949 A DE 3323949A DE 3323949 A1 DE3323949 A1 DE 3323949A1
- Authority
- DE
- Germany
- Prior art keywords
- serum
- molecule
- aqueous
- carbon atoms
- buffered solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 238000000034 method Methods 0.000 title claims description 35
- 239000007788 liquid Substances 0.000 title claims description 5
- 239000008366 buffered solution Substances 0.000 claims abstract description 17
- 150000003626 triacylglycerols Polymers 0.000 claims abstract description 13
- 239000013060 biological fluid Substances 0.000 claims abstract description 9
- 239000000203 mixture Substances 0.000 claims description 26
- 239000003795 chemical substances by application Substances 0.000 claims description 18
- 125000004432 carbon atom Chemical group C* 0.000 claims description 15
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical group [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 11
- 239000000872 buffer Substances 0.000 claims description 10
- 239000002736 nonionic surfactant Substances 0.000 claims description 10
- 239000003945 anionic surfactant Substances 0.000 claims description 9
- 150000003839 salts Chemical class 0.000 claims description 9
- 239000004094 surface-active agent Substances 0.000 claims description 9
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 8
- 125000006353 oxyethylene group Chemical group 0.000 claims description 6
- 229920000151 polyglycol Polymers 0.000 claims description 6
- 239000010695 polyglycol Substances 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 6
- -1 salt sodium chloride Chemical class 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 125000005526 alkyl sulfate group Chemical group 0.000 claims description 5
- PHYFQTYBJUILEZ-IUPFWZBJSA-N triolein Polymers CCCCCCCC\C=C/CCCCCCCC(=O)OCC(OC(=O)CCCCCCC\C=C/CCCCCCCC)COC(=O)CCCCCCC\C=C/CCCCCCCC PHYFQTYBJUILEZ-IUPFWZBJSA-N 0.000 claims description 5
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims description 4
- 229910019142 PO4 Inorganic materials 0.000 claims description 4
- 239000007983 Tris buffer Substances 0.000 claims description 4
- 125000002877 alkyl aryl group Chemical group 0.000 claims description 4
- 150000008055 alkyl aryl sulfonates Chemical class 0.000 claims description 4
- 125000000217 alkyl group Chemical group 0.000 claims description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 4
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 4
- 239000008351 acetate buffer Substances 0.000 claims description 3
- 239000008363 phosphate buffer Substances 0.000 claims description 3
- 239000008362 succinate buffer Substances 0.000 claims description 3
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 claims description 3
- 239000004359 castor oil Substances 0.000 claims description 2
- 235000019438 castor oil Nutrition 0.000 claims description 2
- 150000002170 ethers Chemical class 0.000 claims description 2
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 claims description 2
- 239000010452 phosphate Substances 0.000 claims 1
- 150000003890 succinate salts Chemical group 0.000 claims 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 claims 1
- 125000000129 anionic group Chemical group 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 description 76
- 239000003153 chemical reaction reagent Substances 0.000 description 45
- 239000000523 sample Substances 0.000 description 16
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 12
- 238000010790 dilution Methods 0.000 description 12
- 239000012895 dilution Substances 0.000 description 12
- 102000007592 Apolipoproteins Human genes 0.000 description 10
- 108010071619 Apolipoproteins Proteins 0.000 description 10
- 238000005259 measurement Methods 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 9
- 239000003085 diluting agent Substances 0.000 description 8
- 230000001000 lipidemic effect Effects 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 6
- 239000004480 active ingredient Substances 0.000 description 6
- 230000008030 elimination Effects 0.000 description 6
- 238000003379 elimination reaction Methods 0.000 description 6
- 229910052742 iron Inorganic materials 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 102000009081 Apolipoprotein A-II Human genes 0.000 description 5
- 108010087614 Apolipoprotein A-II Proteins 0.000 description 5
- 239000004615 ingredient Substances 0.000 description 5
- 229920001223 polyethylene glycol Polymers 0.000 description 5
- 102000005666 Apolipoprotein A-I Human genes 0.000 description 4
- 108010059886 Apolipoprotein A-I Proteins 0.000 description 4
- 239000002202 Polyethylene glycol Substances 0.000 description 4
- 108010074605 gamma-Globulins Proteins 0.000 description 4
- 238000001114 immunoprecipitation Methods 0.000 description 4
- 239000011573 trace mineral Substances 0.000 description 4
- 235000013619 trace mineral Nutrition 0.000 description 4
- 241001494479 Pecora Species 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 235000010323 ascorbic acid Nutrition 0.000 description 3
- 229960005070 ascorbic acid Drugs 0.000 description 3
- 239000011668 ascorbic acid Substances 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000011088 calibration curve Methods 0.000 description 3
- 238000005352 clarification Methods 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 230000001900 immune effect Effects 0.000 description 3
- 239000012188 paraffin wax Substances 0.000 description 3
- 210000002381 plasma Anatomy 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000007974 sodium acetate buffer Substances 0.000 description 3
- 102000018616 Apolipoproteins B Human genes 0.000 description 2
- 108010027006 Apolipoproteins B Proteins 0.000 description 2
- 108010004103 Chylomicrons Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004895 Lipoproteins Human genes 0.000 description 2
- 108090001030 Lipoproteins Proteins 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 108010062497 VLDL Lipoproteins Proteins 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003599 detergent Substances 0.000 description 2
- MTHSVFCYNBDYFN-UHFFFAOYSA-N diethylene glycol Chemical compound OCCOCCO MTHSVFCYNBDYFN-UHFFFAOYSA-N 0.000 description 2
- POULHZVOKOAJMA-UHFFFAOYSA-N dodecanoic acid Polymers CCCCCCCCCCCC(O)=O POULHZVOKOAJMA-UHFFFAOYSA-N 0.000 description 2
- GVGUFUZHNYFZLC-UHFFFAOYSA-N dodecyl benzenesulfonate;sodium Chemical compound [Na].CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 GVGUFUZHNYFZLC-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 150000002334 glycols Chemical class 0.000 description 2
- 230000008105 immune reaction Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000008057 potassium phosphate buffer Substances 0.000 description 2
- 229940080264 sodium dodecylbenzenesulfonate Drugs 0.000 description 2
- 159000000000 sodium salts Chemical class 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- JOXIMZWYDAKGHI-UHFFFAOYSA-N toluene-4-sulfonic acid Chemical compound CC1=CC=C(S(O)(=O)=O)C=C1 JOXIMZWYDAKGHI-UHFFFAOYSA-N 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- GZCWLCBFPRFLKL-UHFFFAOYSA-N 1-prop-2-ynoxypropan-2-ol Chemical compound CC(O)COCC#C GZCWLCBFPRFLKL-UHFFFAOYSA-N 0.000 description 1
- NFAOATPOYUWEHM-UHFFFAOYSA-N 2-(6-methylheptyl)phenol Chemical compound CC(C)CCCCCC1=CC=CC=C1O NFAOATPOYUWEHM-UHFFFAOYSA-N 0.000 description 1
- PLLBRTOLHQQAQQ-UHFFFAOYSA-N 8-methylnonan-1-ol Chemical compound CC(C)CCCCCCCO PLLBRTOLHQQAQQ-UHFFFAOYSA-N 0.000 description 1
- 102000013563 Acid Phosphatase Human genes 0.000 description 1
- 108010051457 Acid Phosphatase Proteins 0.000 description 1
- 101710095342 Apolipoprotein B Proteins 0.000 description 1
- 102100040202 Apolipoprotein B-100 Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 101000766307 Gallus gallus Ovotransferrin Proteins 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 102000004338 Transferrin Human genes 0.000 description 1
- 108090000901 Transferrin Proteins 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000001335 aliphatic alkanes Chemical group 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 125000005227 alkyl sulfonate group Chemical group 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000012062 aqueous buffer Substances 0.000 description 1
- 239000012736 aqueous medium Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000012928 buffer substance Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 239000003093 cationic surfactant Substances 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 239000008395 clarifying agent Substances 0.000 description 1
- 239000008139 complexing agent Substances 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- STWJKLMRMTWJEY-UHFFFAOYSA-N diphenyl 1,10-phenanthroline-4,7-disulfonate Chemical compound C=1C=NC(C2=NC=CC(=C2C=C2)S(=O)(=O)OC=3C=CC=CC=3)=C2C=1S(=O)(=O)OC1=CC=CC=C1 STWJKLMRMTWJEY-UHFFFAOYSA-N 0.000 description 1
- DDIHLFCSEHAOJZ-UHFFFAOYSA-L disodium;4-[3-pyridin-2-yl-6-(4-sulfonatophenyl)-1,2,4-triazin-5-yl]benzenesulfonate Chemical compound [Na+].[Na+].C1=CC(S(=O)(=O)[O-])=CC=C1C1=NN=C(C=2N=CC=CC=2)N=C1C1=CC=C(S([O-])(=O)=O)C=C1 DDIHLFCSEHAOJZ-UHFFFAOYSA-L 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 150000002191 fatty alcohols Chemical class 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000001125 hyperlipoproteinemic effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 235000019488 nut oil Nutrition 0.000 description 1
- 239000010466 nut oil Substances 0.000 description 1
- 229920002113 octoxynol Polymers 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- IYDGMDWEHDFVQI-UHFFFAOYSA-N phosphoric acid;trioxotungsten Chemical compound O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.O=[W](=O)=O.OP(O)(O)=O IYDGMDWEHDFVQI-UHFFFAOYSA-N 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 229920000447 polyanionic polymer Polymers 0.000 description 1
- 239000008389 polyethoxylated castor oil Substances 0.000 description 1
- 229940093429 polyethylene glycol 6000 Drugs 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 210000002307 prostate Anatomy 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- RPACBEVZENYWOL-XFULWGLBSA-M sodium;(2r)-2-[6-(4-chlorophenoxy)hexyl]oxirane-2-carboxylate Chemical compound [Na+].C=1C=C(Cl)C=CC=1OCCCCCC[C@]1(C(=O)[O-])CO1 RPACBEVZENYWOL-XFULWGLBSA-M 0.000 description 1
- 239000012581 transferrin Substances 0.000 description 1
- 238000002834 transmittance Methods 0.000 description 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/104165—Lipid, cholesterol, or triglyceride standard or control
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/107497—Preparation composition [e.g., lysing or precipitation, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25125—Digestion or removing interfering materials
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Immunology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Endocrinology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- External Artificial Organs (AREA)
- Infusion, Injection, And Reservoir Apparatuses (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Detergent Compositions (AREA)
Priority Applications (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19833323949 DE3323949A1 (de) | 1983-07-02 | 1983-07-02 | Verfahren und mittel zur raschen und vollstaendigen beseitigung einer truebung in einer biologischen fluessigkeit |
| AT84107339T ATE40478T1 (de) | 1983-07-02 | 1984-06-26 | Verfahren und mittel zur raschen und vollstaendigen beseitigung einer truebung in einer biologischen fluessigkeit. |
| DE8484107339T DE3476461D1 (en) | 1983-07-02 | 1984-06-26 | Method and means for the rapid and complete removal of turbidity from biological fluids |
| EP84107339A EP0130537B1 (de) | 1983-07-02 | 1984-06-26 | Verfahren und Mittel zur raschen und vollständigen Beseitigung einer Trübung in einer biologischen Flüssigkeit |
| CA000457743A CA1229772A (en) | 1983-07-02 | 1984-06-28 | Process and reagent for the complete and rapid removal of a turbidity in a biological fluid |
| ES533881A ES8603993A1 (es) | 1983-07-02 | 1984-06-29 | Procedimiento para obtener un liquido claro biologico de suero o plasma sanguineo de humano. |
| IE1666/84A IE55448B1 (en) | 1983-07-02 | 1984-06-29 | Process and reagent for the complete and rapid removal of a turbidity in a biological fluid |
| DK324384A DK157771C (da) | 1983-07-02 | 1984-07-02 | Fremgangsmaade, middel og anvendelse af dette middel til hurtig og fuldstaendig fjernelse af en uklarhed i en biologisk vaeske |
| US06/626,633 US4579825A (en) | 1983-07-02 | 1984-07-02 | Process for the complete and rapid removal of turbidity in a biological fluid |
| JP59135366A JPS6036959A (ja) | 1983-07-02 | 1984-07-02 | 生物学的液体中の混濁を迅速にかつ完全に除去する方法及びそのための薬剤 |
| US06/803,496 US4708939A (en) | 1983-07-02 | 1985-12-02 | Process and reagent for the complete and rapid removal of a turbidity in a biological fluid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19833323949 DE3323949A1 (de) | 1983-07-02 | 1983-07-02 | Verfahren und mittel zur raschen und vollstaendigen beseitigung einer truebung in einer biologischen fluessigkeit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE3323949A1 true DE3323949A1 (de) | 1985-01-03 |
Family
ID=6203031
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19833323949 Withdrawn DE3323949A1 (de) | 1983-07-02 | 1983-07-02 | Verfahren und mittel zur raschen und vollstaendigen beseitigung einer truebung in einer biologischen fluessigkeit |
| DE8484107339T Expired DE3476461D1 (en) | 1983-07-02 | 1984-06-26 | Method and means for the rapid and complete removal of turbidity from biological fluids |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE8484107339T Expired DE3476461D1 (en) | 1983-07-02 | 1984-06-26 | Method and means for the rapid and complete removal of turbidity from biological fluids |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US4579825A (enExample) |
| EP (1) | EP0130537B1 (enExample) |
| JP (1) | JPS6036959A (enExample) |
| AT (1) | ATE40478T1 (enExample) |
| CA (1) | CA1229772A (enExample) |
| DE (2) | DE3323949A1 (enExample) |
| DK (1) | DK157771C (enExample) |
| ES (1) | ES8603993A1 (enExample) |
| IE (1) | IE55448B1 (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4218555A1 (de) * | 1992-06-05 | 1993-12-09 | Elemer Dipl Chem Dr Faltusz | Verfahren zur Solubilisierung der Milch für Untersuchungszwecke |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3329952A1 (de) * | 1983-08-19 | 1985-02-28 | Behringwerke Ag, 3550 Marburg | Verfahren zur verringerung einer truebung in kontrollseren |
| SE448324B (sv) * | 1985-09-27 | 1987-02-09 | Pharmacia Ab | Sett vid immunkemisk bestemning av lipoproteiner och apolipoproteiner |
| DE3729502A1 (de) * | 1987-09-03 | 1989-03-23 | Boehringer Mannheim Gmbh | Verfahren und reagenz zur bestimmung von eisen |
| IT1216698B (it) * | 1988-04-01 | 1990-03-08 | New Scient Co Spa | Metodo per la determinazione della presenza di catene leggere libere in campioni di urina, complesso di preparati per l'esecuzione del metodo, e relativo reagente. |
| US5219760A (en) * | 1988-05-26 | 1993-06-15 | Boehringer Mannheim Gmbh | Process for the determination of iron |
| DE3817907A1 (de) * | 1988-05-26 | 1989-11-30 | Boehringer Mannheim Gmbh | Verfahren und reagenz zur bestimmung von eisen |
| DE4018502A1 (de) * | 1990-06-09 | 1991-12-12 | Merck Patent Gmbh | Verfahren und mittel zur beseitigung von truebungen in biologischen fluessigkeiten |
| US5844097A (en) * | 1990-11-30 | 1998-12-01 | Monoclonetics International, Inc. | Methods for the diagnosis of peripheral nerve damage |
| AU667462B2 (en) * | 1990-11-30 | 1996-03-28 | Monoclonetics International Incorporated | Methods for the diagnosis of chronic lower back and cervical pain |
| DE69233452T2 (de) * | 1991-09-09 | 2005-12-15 | Canon K.K. | Quantitativer Nachweis vom Lipid, in reiner Form oder im Komplex mit anderen Verbindungen |
| US5286626A (en) * | 1991-12-13 | 1994-02-15 | Actimed Laboratories, Inc. | Process and apparatus for direct determination of low density lipoprotein |
| DE4401754A1 (de) * | 1994-01-21 | 1995-07-27 | Boehringer Mannheim Gmbh | Verfahren und Reagenz zur Bestimmung von Eisen |
| US5962340A (en) * | 1994-11-02 | 1999-10-05 | Wako Pure Chemical Industries Ltd. | Homogeneous immunoassay method utilizing 5-300 mM magnesium |
| US5614414A (en) * | 1995-03-30 | 1997-03-25 | Streck Laboratories, Inc. | Liquid lipoprotein control |
| US5770451A (en) * | 1995-03-30 | 1998-06-23 | Streck Laboratories, Inc. | Liquid lipoprotein control |
| DE19850074A1 (de) * | 1998-10-30 | 2000-05-04 | Dade Behring Marburg Gmbh | Stabilisierung von biologischen Flüssigkeiten durch Zusatz von Sterinestern |
| ATE254767T1 (de) * | 2001-02-19 | 2003-12-15 | Olympus Diagnostica Gmbh | Mittel zur beseitigung von eintrübungen in biologischen proben |
| US7781171B2 (en) * | 2003-10-15 | 2010-08-24 | Daiichi Pure Chemicals Co., Ltd. | Mehod of pretreating sample and immunological assay method using the same |
| JP4876060B2 (ja) * | 2006-12-01 | 2012-02-15 | 積水メディカル株式会社 | 被検試料の非特異的混濁の判別方法及び試薬 |
| EP2847570B1 (en) * | 2012-05-08 | 2020-01-08 | Bentley Instruments S.a.r.l. | Reagent for clarifying emulsions and method of clarification |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3062623A (en) * | 1958-10-13 | 1962-11-06 | Schain Philip | Reagent for the determination of fat in blood serum |
| US3853465A (en) * | 1972-06-09 | 1974-12-10 | Technicon Instr | Turbidity reduction in serum and plasma samples using polyoxyethylated lauric acid compounds |
| US3955925A (en) * | 1973-11-12 | 1976-05-11 | Proksch Gary J | Preparation of optically clear serum |
| US4011045A (en) * | 1975-02-14 | 1977-03-08 | Bonderman Dean P | Turbidity reduction in triglyceride standards |
| DE2724757C2 (de) * | 1977-06-01 | 1979-08-23 | Boehringer Mannheim Gmbh, 6800 Mannheim | Mittel zur Beseitigung von Trübungen in Serum und Verfahren zu seiner Herstellung |
| DE2816229C2 (de) * | 1978-04-14 | 1983-11-10 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren und Mittel zur Beseitigung von Trübungen |
| DE2829531A1 (de) * | 1978-07-05 | 1980-01-24 | Heuck Claus Christian Dr Rer N | Verfahren zur quantitativen bestimmung eines serumproteins in getruebten serum- und plasma-proben |
| US4289469A (en) * | 1980-05-16 | 1981-09-15 | Gloucester Engineering Co., Inc. | Apparatus for forming and trimming articles from a web |
| DE3021457A1 (de) * | 1980-06-06 | 1982-01-07 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren und mittel zur aufloesung von chylomikronen in waessrigem mediums |
| US4503146A (en) * | 1980-10-01 | 1985-03-05 | Technicon Instruments Corporation | Method for eliminating turbidity in a biological fluid and reagent therefor |
| US4400201A (en) * | 1980-12-15 | 1983-08-23 | Sandoz Ltd. | Novel pyrimidinyl ethers, their use as herbicides, herbicidal compositions comprising said pyrimidinyl ethers and processes for the preparation thereof |
| FR2513766B1 (fr) * | 1981-09-28 | 1985-09-13 | Biomerieux Sa | Procedes et reactifs de separation selective des lipoproteines de faible densite ldl et de quantification de leurs composants |
| DE3303098A1 (de) * | 1983-01-31 | 1984-08-02 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren und reagenz zur glucosebestimmung im haemolysierten blut |
-
1983
- 1983-07-02 DE DE19833323949 patent/DE3323949A1/de not_active Withdrawn
-
1984
- 1984-06-26 EP EP84107339A patent/EP0130537B1/de not_active Expired
- 1984-06-26 DE DE8484107339T patent/DE3476461D1/de not_active Expired
- 1984-06-26 AT AT84107339T patent/ATE40478T1/de active
- 1984-06-28 CA CA000457743A patent/CA1229772A/en not_active Expired
- 1984-06-29 IE IE1666/84A patent/IE55448B1/en not_active IP Right Cessation
- 1984-06-29 ES ES533881A patent/ES8603993A1/es not_active Expired
- 1984-07-02 DK DK324384A patent/DK157771C/da not_active IP Right Cessation
- 1984-07-02 JP JP59135366A patent/JPS6036959A/ja active Granted
- 1984-07-02 US US06/626,633 patent/US4579825A/en not_active Expired - Lifetime
-
1985
- 1985-12-02 US US06/803,496 patent/US4708939A/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4218555A1 (de) * | 1992-06-05 | 1993-12-09 | Elemer Dipl Chem Dr Faltusz | Verfahren zur Solubilisierung der Milch für Untersuchungszwecke |
Also Published As
| Publication number | Publication date |
|---|---|
| DE3476461D1 (en) | 1989-03-02 |
| US4579825A (en) | 1986-04-01 |
| EP0130537B1 (de) | 1989-01-25 |
| ES8603993A1 (es) | 1986-01-16 |
| ES533881A0 (es) | 1986-01-16 |
| DK157771B (da) | 1990-02-12 |
| EP0130537A2 (de) | 1985-01-09 |
| ATE40478T1 (de) | 1989-02-15 |
| DK324384D0 (da) | 1984-07-02 |
| US4708939A (en) | 1987-11-24 |
| CA1229772A (en) | 1987-12-01 |
| DK324384A (da) | 1985-01-03 |
| DK157771C (da) | 1990-07-09 |
| JPH0316620B2 (enExample) | 1991-03-06 |
| JPS6036959A (ja) | 1985-02-26 |
| EP0130537A3 (en) | 1986-02-05 |
| IE55448B1 (en) | 1990-09-12 |
| IE841666L (en) | 1985-01-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0130537B1 (de) | Verfahren und Mittel zur raschen und vollständigen Beseitigung einer Trübung in einer biologischen Flüssigkeit | |
| EP0265933B1 (de) | Verfahren und Reagenz zur spezifischen Bestimmung des Cholesterins der HDL-Fraktion | |
| EP0141343B1 (de) | Verfahren zur Bestimmung der Low Density Lipoproteine (LDL) und Reagenz zu seiner Durchführung | |
| DE69623174T2 (de) | Verfahren und Reagenszusammensetzung zur Durchführung von differenzierenden Leukocytzählungen in frischen und gealteren Vollblutproben, auf der Grundlage der bereitsvorhandenen Peroxidaseaktivität der Leukocyten | |
| DE69621459T2 (de) | Reagens und verfahren zur differentiellen bestimmung von leukozyten im blut | |
| DE2153479A1 (de) | Verfahren und Vorrichtung zum differentiellen Zählen von weißen Blutzellen | |
| EP0092801A1 (de) | Verfahren zur Bestimmung der Low Density Lipoproteine (LDL) und Reagenz zu seiner Durchführung | |
| DE69713498T2 (de) | Methode zum nachweis übertragbarer spongiformer enzephalopathien | |
| EP0012184A1 (de) | Hämolysierlösung, Verwendung einer solchen für die Bestimmung von Glucose im Blut, und Hämolyseverfahren | |
| DE60028776T2 (de) | Verfahren zum vorbehandeln von proben und zum quantifizieren von cholesterin in spezifischen lipoproteinen | |
| DE69333586T2 (de) | Verfahren und zusammensetzung zur konservierung von antigenen und verfahren zur verwendung von durch selbiges verfahren hergestellten zytologischem material | |
| DE3854326T2 (de) | Aufbereitungstechnik für vollblutmuster. | |
| EP0008338A1 (de) | Verfahren zur quantitativen Bestimmung eines Serumproteins in getrübten Serum- und Plasma-Proben und Mittel zur Durchführung des Verfahrens | |
| DE19505894A1 (de) | Verfahren und Reagenz zur spezifischen Bestimmung von LDL in Serumproben | |
| EP1052512B1 (de) | Verfahren und Teilreagenz zur Bestimmung von Eisen in Serum | |
| DE19756255C2 (de) | Verfahren und Diagnostikprodukt zur Bestimmung von in Lipoprotein enthaltenem Triglycerid | |
| EP0039885A1 (de) | Verfahren und Reagens zum Nachweis von Fibrinmonomer | |
| EP0238914A1 (de) | Verfahren und Reagenz zur Bestimmung von Gesamt-Bilirubin | |
| DE2608733A1 (de) | Reagens zur bestimmung von fibrinogen, fibrinspaltprodukten und fibrinogenspaltprodukten im blut und verfahren zur herstellung desselben | |
| DE60101254T2 (de) | Mittel zur Beseitigung von Eintrübungen in biologischen Proben | |
| DE69332764T2 (de) | Hydroxylamin enthaltendes kontrollreagenz | |
| DE3920364C2 (enExample) | ||
| EP0461473B1 (de) | Verfahren und Mittel zur Beseitigung von Trübungen in biologischen Flüssigkeiten | |
| CH657919A5 (de) | Reagens zur beseitigung einer truebung in einer biologischen fluessigkeit. | |
| EP0041704B1 (de) | Verfahren und Mittel zur Auflösung von Chylomikronen in wässrigem Medium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8139 | Disposal/non-payment of the annual fee |