DE3211314C2 - Verfahren zur Ermittlung von Kennwerten von Suspensionen n-Paraffine oxidierender Hefen - Google Patents
Verfahren zur Ermittlung von Kennwerten von Suspensionen n-Paraffine oxidierender HefenInfo
- Publication number
- DE3211314C2 DE3211314C2 DE19823211314 DE3211314A DE3211314C2 DE 3211314 C2 DE3211314 C2 DE 3211314C2 DE 19823211314 DE19823211314 DE 19823211314 DE 3211314 A DE3211314 A DE 3211314A DE 3211314 C2 DE3211314 C2 DE 3211314C2
- Authority
- DE
- Germany
- Prior art keywords
- cells
- histogram
- suspensions
- dead
- brightness
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 63
- 239000000725 suspension Substances 0.000 title claims abstract description 59
- 238000000034 method Methods 0.000 title claims abstract description 49
- 239000012188 paraffin wax Substances 0.000 title claims description 31
- 230000001590 oxidative effect Effects 0.000 title 1
- 238000002360 preparation method Methods 0.000 claims abstract description 31
- 239000011159 matrix material Substances 0.000 claims abstract description 9
- 150000002632 lipids Chemical class 0.000 claims description 42
- 238000000354 decomposition reaction Methods 0.000 claims description 6
- 238000005375 photometry Methods 0.000 claims description 5
- 239000002245 particle Substances 0.000 abstract description 6
- 239000006285 cell suspension Substances 0.000 abstract description 4
- 239000007962 solid dispersion Substances 0.000 abstract description 2
- 239000000839 emulsion Substances 0.000 abstract 2
- 238000011835 investigation Methods 0.000 abstract 2
- 239000006185 dispersion Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 93
- 239000001963 growth medium Substances 0.000 description 12
- 238000004458 analytical method Methods 0.000 description 6
- 210000005253 yeast cell Anatomy 0.000 description 6
- 238000009630 liquid culture Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 4
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 3
- 229960000907 methylthioninium chloride Drugs 0.000 description 3
- 230000002906 microbiologic effect Effects 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 238000003113 dilution method Methods 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 239000010410 layer Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004020 luminiscence type Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- YDIKCZBMBPOGFT-PWUSVEHZSA-N Malvidin 3-galactoside Chemical compound [Cl-].COC1=C(O)C(OC)=CC(C=2C(=CC=3C(O)=CC(O)=CC=3[O+]=2)O[C@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)=C1 YDIKCZBMBPOGFT-PWUSVEHZSA-N 0.000 description 1
- 230000010718 Oxidation Activity Effects 0.000 description 1
- PXUQTDZNOHRWLI-QOPOCTTISA-O Primulin Natural products O(C)c1c(O)c(OC)cc(-c2c(O[C@H]3[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O3)cc3c(O)cc(O)cc3[o+]2)c1 PXUQTDZNOHRWLI-QOPOCTTISA-O 0.000 description 1
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 1
- DPKHZNPWBDQZCN-UHFFFAOYSA-N acridine orange free base Chemical compound C1=CC(N(C)C)=CC2=NC3=CC(N(C)C)=CC=C3C=C21 DPKHZNPWBDQZCN-UHFFFAOYSA-N 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Natural products C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 1
- 239000013590 bulk material Substances 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000005337 ground glass Substances 0.000 description 1
- 229930195733 hydrocarbon Natural products 0.000 description 1
- 150000002430 hydrocarbons Chemical class 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- -1 isolated dead cells Substances 0.000 description 1
- 238000005088 metallography Methods 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000002135 phase contrast microscopy Methods 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1468—Optical investigation techniques, e.g. flow cytometry with spatial resolution of the texture or inner structure of the particle
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19823211314 DE3211314C2 (de) | 1982-03-26 | 1982-03-26 | Verfahren zur Ermittlung von Kennwerten von Suspensionen n-Paraffine oxidierender Hefen |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19823211314 DE3211314C2 (de) | 1982-03-26 | 1982-03-26 | Verfahren zur Ermittlung von Kennwerten von Suspensionen n-Paraffine oxidierender Hefen |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE3211314A1 DE3211314A1 (de) | 1983-09-29 |
| DE3211314C2 true DE3211314C2 (de) | 1987-04-23 |
Family
ID=6159461
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19823211314 Expired DE3211314C2 (de) | 1982-03-26 | 1982-03-26 | Verfahren zur Ermittlung von Kennwerten von Suspensionen n-Paraffine oxidierender Hefen |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE3211314C2 (fr) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2510771B2 (ja) * | 1990-07-25 | 1996-06-26 | 株式会社日立製作所 | 培養生体の活性診断方法及びシステム |
| GB2254414A (en) * | 1991-03-21 | 1992-10-07 | Univ London | Volume measurement of microbial organisms. |
| GB2578299A (en) * | 2018-10-19 | 2020-05-06 | Institute Of Tech Sligo | Systems for controlling an emulsification process |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2449586A (en) * | 1942-08-15 | 1948-09-21 | Borg Warner | Transmission and control therefor |
| US3720837A (en) * | 1971-12-20 | 1973-03-13 | Miles Lab | Measuring and indicating apparatus |
| JPS5126595A (en) * | 1974-08-29 | 1976-03-04 | Shimadzu Corp | Denshitomeeta no beesurainhoseisochi |
| DE2741321C3 (de) * | 1977-09-14 | 1981-05-27 | Wibau Industrie und Verwaltung GmbH, 6466 Gründau | Verfahren zum Bestimmen der Teilchengrößenverteilung eines fallenden oder fließenden Gutstromes durch Aufnehmen und Auswerten von Videobildern |
-
1982
- 1982-03-26 DE DE19823211314 patent/DE3211314C2/de not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| DE3211314A1 (de) | 1983-09-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OM8 | Search report available as to paragraph 43 lit. 1 sentence 1 patent law | ||
| 8110 | Request for examination paragraph 44 | ||
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |