DE2924249C2 - Spezifische bindungs-untersuchungsmethode zur bestimmung eines liganden in einem fluessigen medium und reagentien zur durchfuehrung dieser methode - Google Patents

Spezifische bindungs-untersuchungsmethode zur bestimmung eines liganden in einem fluessigen medium und reagentien zur durchfuehrung dieser methode

Info

Publication number
DE2924249C2
DE2924249C2 DE2924249A DE2924249A DE2924249C2 DE 2924249 C2 DE2924249 C2 DE 2924249C2 DE 2924249 A DE2924249 A DE 2924249A DE 2924249 A DE2924249 A DE 2924249A DE 2924249 C2 DE2924249 C2 DE 2924249C2
Authority
DE
Germany
Prior art keywords
ligand
binding
labeled conjugate
apoenzyme
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE2924249A
Other languages
German (de)
English (en)
Other versions
DE2924249A1 (de
Inventor
William E Hornby
David L Morris
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Miles Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miles Inc filed Critical Miles Inc
Publication of DE2924249A1 publication Critical patent/DE2924249A1/de
Application granted granted Critical
Publication of DE2924249C2 publication Critical patent/DE2924249C2/de
Expired legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Prostheses (AREA)
DE2924249A 1978-06-22 1979-06-15 Spezifische bindungs-untersuchungsmethode zur bestimmung eines liganden in einem fluessigen medium und reagentien zur durchfuehrung dieser methode Expired DE2924249C2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US91796178A 1978-06-22 1978-06-22

Publications (2)

Publication Number Publication Date
DE2924249A1 DE2924249A1 (de) 1980-01-03
DE2924249C2 true DE2924249C2 (de) 1989-04-27

Family

ID=25439569

Family Applications (1)

Application Number Title Priority Date Filing Date
DE2924249A Expired DE2924249C2 (de) 1978-06-22 1979-06-15 Spezifische bindungs-untersuchungsmethode zur bestimmung eines liganden in einem fluessigen medium und reagentien zur durchfuehrung dieser methode

Country Status (21)

Country Link
JP (1) JPS6145776B2 (xx)
AR (1) AR220947A1 (xx)
AT (1) AT363615B (xx)
AU (1) AU530673B2 (xx)
BE (1) BE877060A (xx)
BR (1) BR7903958A (xx)
CH (1) CH659713A5 (xx)
DD (1) DD144461A5 (xx)
DE (1) DE2924249C2 (xx)
DK (1) DK154670C (xx)
ES (1) ES481782A1 (xx)
FI (1) FI70726C (xx)
FR (1) FR2429258B1 (xx)
GB (1) GB2023607B (xx)
IE (1) IE48264B1 (xx)
IL (1) IL57570A (xx)
LU (1) LU81410A1 (xx)
NL (1) NL181527C (xx)
NO (1) NO154447C (xx)
SE (1) SE436522B (xx)
ZA (1) ZA793068B (xx)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1183450A (en) * 1980-10-30 1985-03-05 Alfred C. Greenquist Homogeneous specific binding assay device and method
US4378428A (en) * 1981-03-30 1983-03-29 Baker Instruments Corporation Method for carrying out non-isotopic immunoassays, labeled analytes and kits for use in such assays
AU574646B2 (en) * 1982-07-19 1988-07-14 Cooperbiomedical Inc. Enzyme assay method
US4550075A (en) * 1983-06-22 1985-10-29 Kallestad Laboratories, Inc. Method for ligand determination utilizing an immunoassay monitorable by biotin-containing enzymes, and compositions therefor
FR2556726B1 (fr) * 1983-12-20 1987-02-20 California Inst Of Techn Compositions a base d'oligonucleotides monocatenaires et procede pour leur preparation
GB8608435D0 (en) * 1986-04-07 1986-05-14 Cranfield Inst Of Tech Specific binding assays
EP0274343A1 (en) * 1987-01-06 1988-07-13 IntraCel Corporation A system of reactants involving an apo-enzyme useful in immunological analysis and a method for carrying out immunoassays with this system
NL8800820A (nl) * 1988-03-31 1989-10-16 Philips Nv Cassette.
US7341830B2 (en) * 2002-05-16 2008-03-11 Roche Diagnostics Operations, Inc. Method and reagent system having a non-regenerative enzyme-coenzyme complex
US7759072B2 (en) 2005-03-03 2010-07-20 Sekisui Medical Co., Ltd. Immunoassay method and reagent therefor
FR2889873B1 (fr) 2005-08-18 2007-11-16 Ceva Sante Animale Sa Methode de detection de la progesterone dans le lait de vache et kit correspondant
EP3576129B1 (en) * 2018-06-01 2023-05-03 Thermo Fisher Scientific (Bremen) GmbH Method for detecting the isotopic labelling state of unknown species of molecules

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN142734B (xx) * 1975-04-28 1977-08-20 Miles Lab

Also Published As

Publication number Publication date
FR2429258A1 (fr) 1980-01-18
DD144461A5 (de) 1980-10-15
IL57570A0 (en) 1979-10-31
GB2023607B (en) 1983-01-06
DK154670B (da) 1988-12-05
DE2924249A1 (de) 1980-01-03
NO154447C (no) 1986-09-17
AU530673B2 (en) 1983-07-28
IE48264B1 (en) 1984-11-14
FI70726C (fi) 1986-10-06
AU4823079A (en) 1980-01-03
NL7904879A (nl) 1979-12-28
BE877060A (fr) 1979-10-15
IL57570A (en) 1982-07-30
AR220947A1 (es) 1980-12-15
AT363615B (de) 1981-08-25
IE791156L (en) 1979-12-22
NL181527C (nl) 1987-09-01
FR2429258B1 (fr) 1985-03-29
GB2023607A (en) 1980-01-03
CH659713A5 (de) 1987-02-13
SE436522B (sv) 1984-12-17
NO792084L (no) 1979-12-28
SE7905514L (sv) 1979-12-23
JPS6145776B2 (en) 1986-10-09
DK154670C (da) 1989-05-01
ES481782A1 (es) 1980-07-01
ZA793068B (en) 1980-08-27
FI70726B (fi) 1986-06-26
BR7903958A (pt) 1980-02-12
JPS552997A (en) 1980-01-10
FI791937A (fi) 1979-12-23
NO154447B (no) 1986-06-09
ATA438979A (de) 1981-01-15
DK258079A (da) 1979-12-23
LU81410A1 (fr) 1979-10-30

Similar Documents

Publication Publication Date Title
DE2754086C3 (de) Spezifisches Bindungsverfahren zum Nachweis und zur Bestimmung eines Liganden in einem flüssigen Medium
DE3781170T2 (de) Hydrolysierbare fluoreszierende substrate und deren verwendung zur analytischen bestimmung.
DE69304511T2 (de) Testverfahren für homocysteine
DE68910758T2 (de) Bestimmung mit Affinitätssubstanzen und HPLC-Schritt.
US4238565A (en) Specific binding assay with a prosthetic group as a label component
DE69902265T2 (de) Redox-reversible imidazol-osmiumkomplex-konjugate
EP0280211B1 (de) Verfahren zur Bestimmung von Antikörpern
DE2618419C2 (de) Heterogenes spezifisches Bindungsverfahren zur Bestimmung eines Liganden in einem flüssigen Medium
EP0243429B1 (de) D-luciferin-derivate und deren verwendung
DE2924249C2 (de) Spezifische bindungs-untersuchungsmethode zur bestimmung eines liganden in einem fluessigen medium und reagentien zur durchfuehrung dieser methode
DE2206103A1 (de) Verfahren zum Nachweisen und Bestimmen einer Komponente der Reaktion zwischen spezifisch bindenden Proteinen und den Substanzen, die von diesen Proteinen spezifisch gebunden werden
DE3006118C2 (xx)
US4213893A (en) Flavin adenine dinucleotide-labeled conjugates for use in specific binding assays
US4318981A (en) Homogeneous specific binding assay employing an intramolecularly modulated photogenic enzyme substrate label
US4376165A (en) Method of preparing an enzyme-labeled ligand for use in specific binding assays and the labeled conjugate produced thereby
EP0749435B1 (de) Entstörmittel zum einsatz bei immunoassays
US4255566A (en) Flavin adenine dinucleotide derivatives and labeled conjugates prepared therefrom
DE69636013T2 (de) Verfahren zum erlangen von rezeptor-freigabeligand (reland)-komplexen und testansätze
US4318982A (en) FMN-Labeled specific binding assay
US4318983A (en) Fad-labeled specific binding assay monitored with apoglutathione reductase or apolipoamide dehydrogenase
EP0374620A2 (de) Verfahren und Mittel zur Bestimmung von Maleimidgruppen
US4340668A (en) Heme-labeled specific binding assay
DE2924332C2 (xx)
EP0465998B1 (de) Neue Beta-Galactosidase-Substrate für den CEDIA
DE69120513T2 (de) Verfahren zur schnellen Messung von Spurbestandteilen

Legal Events

Date Code Title Description
OAP Request for examination filed
OD Request for examination
8127 New person/name/address of the applicant

Owner name: MILES, INC., ELKHART, IND., US

8128 New person/name/address of the agent

Representative=s name: MUELLER, G., DIPL.-CHEM. DR.RER.NAT., PAT.-ASS., 5

D2 Grant after examination
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee