DE2855698A1 - Verfahren zur herstellung eines urokinase mit einem molekulargewicht von 54 000 + 10 000 enthaltenden praeparats und seine verwendung als fibrinolytikum und thrombolytikum - Google Patents
Verfahren zur herstellung eines urokinase mit einem molekulargewicht von 54 000 + 10 000 enthaltenden praeparats und seine verwendung als fibrinolytikum und thrombolytikumInfo
- Publication number
- DE2855698A1 DE2855698A1 DE19782855698 DE2855698A DE2855698A1 DE 2855698 A1 DE2855698 A1 DE 2855698A1 DE 19782855698 DE19782855698 DE 19782855698 DE 2855698 A DE2855698 A DE 2855698A DE 2855698 A1 DE2855698 A1 DE 2855698A1
- Authority
- DE
- Germany
- Prior art keywords
- urokinase
- molecular weight
- activity
- solution
- aqueous
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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- 108090000435 Urokinase-type plasminogen activator Proteins 0.000 title claims abstract description 139
- 229960005356 urokinase Drugs 0.000 title claims abstract description 133
- 230000007935 neutral effect Effects 0.000 title claims description 6
- 239000003146 anticoagulant agent Substances 0.000 title claims description 5
- 229960004676 antithrombotic agent Drugs 0.000 title 1
- 239000000243 solution Substances 0.000 claims description 38
- 238000000034 method Methods 0.000 claims description 29
- 238000002360 preparation method Methods 0.000 claims description 23
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- 230000002537 thrombolytic effect Effects 0.000 claims description 17
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- OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 description 1
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- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6462—Plasminogen activators u-Plasminogen activator (3.4.21.73), i.e. urokinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21073—Serine endopeptidases (3.4.21) u-Plasminogen activator (3.4.21.73), i.e. urokinase
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19782855698 DE2855698A1 (de) | 1978-12-22 | 1978-12-22 | Verfahren zur herstellung eines urokinase mit einem molekulargewicht von 54 000 + 10 000 enthaltenden praeparats und seine verwendung als fibrinolytikum und thrombolytikum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19782855698 DE2855698A1 (de) | 1978-12-22 | 1978-12-22 | Verfahren zur herstellung eines urokinase mit einem molekulargewicht von 54 000 + 10 000 enthaltenden praeparats und seine verwendung als fibrinolytikum und thrombolytikum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE2855698A1 true DE2855698A1 (de) | 1980-07-03 |
| DE2855698C2 DE2855698C2 (en, 2012) | 1988-07-21 |
Family
ID=6058136
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19782855698 Granted DE2855698A1 (de) | 1978-12-22 | 1978-12-22 | Verfahren zur herstellung eines urokinase mit einem molekulargewicht von 54 000 + 10 000 enthaltenden praeparats und seine verwendung als fibrinolytikum und thrombolytikum |
Country Status (1)
| Country | Link |
|---|---|
| DE (1) | DE2855698A1 (en, 2012) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0295940A3 (en) * | 1987-06-18 | 1989-05-31 | Nihon Chemical Research Kabushiki Kaisha Also Known As Japan Chemical Research Co., Ltd | Method of suppressing the thermal degradation of urokinase |
| EP0299715A3 (en) * | 1987-07-13 | 1990-07-04 | MITSUI TOATSU CHEMICALS, Inc. | Process for preparing L-tryptophan |
| DE19856443A1 (de) * | 1998-12-08 | 2000-06-21 | Centeon Pharma Gmbh | Stabilisiertes Antithrombin III-Präparat |
-
1978
- 1978-12-22 DE DE19782855698 patent/DE2855698A1/de active Granted
Non-Patent Citations (1)
| Title |
|---|
| Methods in Enzymology, Vol. XIX, S. 667-671, 1970 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0295940A3 (en) * | 1987-06-18 | 1989-05-31 | Nihon Chemical Research Kabushiki Kaisha Also Known As Japan Chemical Research Co., Ltd | Method of suppressing the thermal degradation of urokinase |
| EP0299715A3 (en) * | 1987-07-13 | 1990-07-04 | MITSUI TOATSU CHEMICALS, Inc. | Process for preparing L-tryptophan |
| DE19856443A1 (de) * | 1998-12-08 | 2000-06-21 | Centeon Pharma Gmbh | Stabilisiertes Antithrombin III-Präparat |
Also Published As
| Publication number | Publication date |
|---|---|
| DE2855698C2 (en, 2012) | 1988-07-21 |
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