DE2747853C2 - Verfahren zum quantitativen Messen von angiotensinumwandelnder Enzymaktivität - Google Patents

Verfahren zum quantitativen Messen von angiotensinumwandelnder Enzymaktivität

Info

Publication number
DE2747853C2
DE2747853C2 DE2747853A DE2747853A DE2747853C2 DE 2747853 C2 DE2747853 C2 DE 2747853C2 DE 2747853 A DE2747853 A DE 2747853A DE 2747853 A DE2747853 A DE 2747853A DE 2747853 C2 DE2747853 C2 DE 2747853C2
Authority
DE
Germany
Prior art keywords
substrate
enzyme
peptide
product
reaction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE2747853A
Other languages
German (de)
English (en)
Other versions
DE2747853A1 (de
Inventor
Alfred Miami Fla. Chung
James Walter Ryan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
VENTREX LABORATORIES Inc PORTLAND ME US
Original Assignee
VENTREX LABORATORIES Inc PORTLAND ME US
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by VENTREX LABORATORIES Inc PORTLAND ME US filed Critical VENTREX LABORATORIES Inc PORTLAND ME US
Publication of DE2747853A1 publication Critical patent/DE2747853A1/de
Application granted granted Critical
Publication of DE2747853C2 publication Critical patent/DE2747853C2/de
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/08—Tripeptides
    • C07K5/0821—Tripeptides with the first amino acid being heterocyclic, e.g. His, Pro, Trp
    • C07K5/0823—Tripeptides with the first amino acid being heterocyclic, e.g. His, Pro, Trp and Pro-amino acid; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/08—Tripeptides
    • C07K5/0802—Tripeptides with the first amino acid being neutral
    • C07K5/0804—Tripeptides with the first amino acid being neutral and aliphatic
    • C07K5/0806—Tripeptides with the first amino acid being neutral and aliphatic the side chain containing 0 or 1 carbon atoms, i.e. Gly, Ala
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96486—Metalloendopeptidases (3.4.24)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
DE2747853A 1977-05-10 1977-10-26 Verfahren zum quantitativen Messen von angiotensinumwandelnder Enzymaktivität Expired DE2747853C2 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US05/795,497 US4115374A (en) 1977-05-10 1977-05-10 Substrates for angiotensin converting enzyme

Publications (2)

Publication Number Publication Date
DE2747853A1 DE2747853A1 (de) 1978-11-23
DE2747853C2 true DE2747853C2 (de) 1986-11-13

Family

ID=25165666

Family Applications (3)

Application Number Title Priority Date Filing Date
DE2760343A Expired - Fee Related DE2760343C2 (US08066781-20111129-C00013.png) 1977-05-10 1977-10-26
DE2760342A Expired - Fee Related DE2760342C2 (US08066781-20111129-C00013.png) 1977-05-10 1977-10-26
DE2747853A Expired DE2747853C2 (de) 1977-05-10 1977-10-26 Verfahren zum quantitativen Messen von angiotensinumwandelnder Enzymaktivität

Family Applications Before (2)

Application Number Title Priority Date Filing Date
DE2760343A Expired - Fee Related DE2760343C2 (US08066781-20111129-C00013.png) 1977-05-10 1977-10-26
DE2760342A Expired - Fee Related DE2760342C2 (US08066781-20111129-C00013.png) 1977-05-10 1977-10-26

Country Status (4)

Country Link
US (1) US4115374A (US08066781-20111129-C00013.png)
CA (1) CA1129847A (US08066781-20111129-C00013.png)
DE (3) DE2760343C2 (US08066781-20111129-C00013.png)
GB (1) GB1555832A (US08066781-20111129-C00013.png)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4251628A (en) * 1978-03-14 1981-02-17 Ryan James W Substrates for angiotensin converting enzyme
US4260682A (en) * 1979-04-04 1981-04-07 Ryan James W High sensitivity assays for angiotensin converting enzyme
US4327178A (en) * 1979-05-01 1982-04-27 University Of Miami Urinary kallikrein assay: specific substrates and assay method
US4372941A (en) * 1980-09-05 1983-02-08 Ryan James W Novel radioassay procedure
JPS5935597B2 (ja) * 1980-12-23 1984-08-29 富士レビオ株式会社 アンジオテンシン変換酵素の活性測定法
US4563305A (en) * 1981-01-07 1986-01-07 University Of Miami Radiolabelled substrates for assaying mammalian enzymes
US4448715A (en) * 1981-11-02 1984-05-15 University Of Miami Tagged pyroglu-L-Phe-L-Arg derivatives, substrates and assays for kallikrein
US5045303A (en) * 1985-05-17 1991-09-03 Neorx Corporation Radiohalogenated small molecules for protein labeling
JPS62277331A (ja) * 1986-04-21 1987-12-02 ネオルツクス　コ−ポレイシヨン 蛋白質標識のための放射性ハロゲン化小分子
US4883862A (en) * 1988-04-13 1989-11-28 Albert Einstein College Of Medicine - Of Yeshiva University Mercaptosuccinyl glycyl-glycyl-glycine a complex thereof with Tc-99m, and methods of making the same
CN2409803Y (zh) * 1999-12-21 2000-12-13 黄义全 导流通风安全帽

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4057629A (en) * 1974-12-18 1977-11-08 Daiichi Seiyaku Co., Ltd. Tyrosine derivatives and process for preparing the same

Also Published As

Publication number Publication date
DE2760342A1 (US08066781-20111129-C00013.png) 1986-03-20
US4115374A (en) 1978-09-19
GB1555832A (en) 1979-11-14
DE2760343A1 (US08066781-20111129-C00013.png) 1986-03-20
DE2760343C2 (US08066781-20111129-C00013.png) 1990-06-28
DE2760342C2 (US08066781-20111129-C00013.png) 1990-06-28
CA1129847A (en) 1982-08-17
DE2747853A1 (de) 1978-11-23

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