DE2731421A1 - Verfahren zur bestimmung des amylasegehalts von proben - Google Patents

Verfahren zur bestimmung des amylasegehalts von proben

Info

Publication number
DE2731421A1
DE2731421A1 DE19772731421 DE2731421A DE2731421A1 DE 2731421 A1 DE2731421 A1 DE 2731421A1 DE 19772731421 DE19772731421 DE 19772731421 DE 2731421 A DE2731421 A DE 2731421A DE 2731421 A1 DE2731421 A1 DE 2731421A1
Authority
DE
Germany
Prior art keywords
substrate
maltase
radical
nitrophenyl
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
DE19772731421
Other languages
German (de)
English (en)
Other versions
DE2731421C2 (cs
Inventor
Richard Charles Burns
Robert Carroll Menson
Venkatachalam Narayanswamy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of DE2731421A1 publication Critical patent/DE2731421A1/de
Application granted granted Critical
Publication of DE2731421C2 publication Critical patent/DE2731421C2/de
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/10—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases p-Nitrophenol derivatives

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE19772731421 1976-07-13 1977-07-12 Verfahren zur bestimmung des amylasegehalts von proben Granted DE2731421A1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US70497676A 1976-07-13 1976-07-13

Publications (2)

Publication Number Publication Date
DE2731421A1 true DE2731421A1 (de) 1978-02-09
DE2731421C2 DE2731421C2 (cs) 1988-07-07

Family

ID=24831606

Family Applications (1)

Application Number Title Priority Date Filing Date
DE19772731421 Granted DE2731421A1 (de) 1976-07-13 1977-07-12 Verfahren zur bestimmung des amylasegehalts von proben

Country Status (12)

Country Link
JP (1) JPS5311092A (cs)
BE (1) BE856737A (cs)
CA (1) CA1104912A (cs)
CH (1) CH633582A5 (cs)
DE (1) DE2731421A1 (cs)
DK (1) DK155751C (cs)
FR (1) FR2358660A1 (cs)
GB (1) GB1571642A (cs)
IE (1) IE45395B1 (cs)
IT (1) IT1114891B (cs)
LU (1) LU77751A1 (cs)
NL (1) NL179399C (cs)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2752501A1 (de) * 1977-11-24 1979-05-31 Kurt Prof Dr Wallenfels Mit indikatorgruppen substituierte alpha-d-maltodextrine, verfahren zu deren herstellung und ihre verwendung zur bestimmung von glukosidasen und amylasen
DE2755803A1 (de) * 1977-12-14 1979-06-21 Boehringer Mannheim Gmbh Verfahren und reagens zur bestimmung von alpha-amylase

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4102747A (en) * 1977-07-28 1978-07-25 American Hospital Supply Corporation Amylase determination
DE2741192C2 (de) * 1977-09-13 1982-07-01 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur Bestimmung von alpha- Amylase
AT362526B (de) * 1977-09-13 1981-05-25 Boehringer Mannheim Gmbh Verfahren und reagens zur bestimmung von alpha- -amylase
JPS5768798A (en) * 1980-10-14 1982-04-27 Toyo Jozo Co Ltd Novel measurement of amylase activity
DE3328616A1 (de) * 1983-08-08 1985-02-28 Boehringer Mannheim Gmbh, 6800 Mannheim Oligoglucosidderivate
US4649108A (en) * 1984-07-26 1987-03-10 Genzyme Corporation Alpha amylase assay
US4963479A (en) * 1986-10-07 1990-10-16 Hoechst Celanese Corporation Reagent system for an alpha-amylase assay containing aromatic substituted glycoside

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1643196A1 (de) * 1966-01-20 1972-03-23 Warner Lambert Pharmaceutical Verfahren zur Herstellung eines Praeparats zur Amylasegehalts-Bestimmung
US3676303A (en) * 1968-01-15 1972-07-11 Pharmacia Ab Method for determining endohydrolases capable of breaking down polysaccharides and reagents for carrying out the method
DE2602542A1 (de) * 1975-01-24 1976-07-29 Ono Pharmaceutical Co Verfahren zur quantitativen bestimmung von glucoamylase im menschlichen urin und in koerperfluessigkeiten
DE2442561B2 (de) * 1973-09-06 1978-01-12 E.I. du Pont de Nemours and Co, Wilmington, Del. (V.St.A.) Diagnostikum und verfahren zur bestimmung des alpha-amylasegehalts einer probe

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2094693A (en) * 1935-06-17 1937-10-05 Trojan Powder Co Nitration of sugars and their glycosides
FR1508496A (fr) * 1966-01-20 1968-01-05 Warner Lambert Pharmaceutical Produit et procédé pour le dosage de l'amylase
CA973499A (en) * 1970-07-28 1975-08-26 Hayashibara, Ken Process for the preparation of amylose as the substrate for the quantitative analysis of amylose
NL188646C (nl) * 1976-07-13 1992-08-17 Du Pont Werkwijze voor het bereiden van nitroaromatische glycosiden.

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE1643196A1 (de) * 1966-01-20 1972-03-23 Warner Lambert Pharmaceutical Verfahren zur Herstellung eines Praeparats zur Amylasegehalts-Bestimmung
US3676303A (en) * 1968-01-15 1972-07-11 Pharmacia Ab Method for determining endohydrolases capable of breaking down polysaccharides and reagents for carrying out the method
DE2442561B2 (de) * 1973-09-06 1978-01-12 E.I. du Pont de Nemours and Co, Wilmington, Del. (V.St.A.) Diagnostikum und verfahren zur bestimmung des alpha-amylasegehalts einer probe
DE2602542A1 (de) * 1975-01-24 1976-07-29 Ono Pharmaceutical Co Verfahren zur quantitativen bestimmung von glucoamylase im menschlichen urin und in koerperfluessigkeiten

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BARMAN, T.E.: Enzyme Handbood, Vol.II, S.560-561, Springer-Verlag, Berlin 1969 *
BERGMEYER, H.U.: Methoden der enzymatischen Analyse, 3.Aufl., Bd.I, S.923, Verlag Chemie, Weinheim 1974 *
JANSEN et al., ?-(p-Nitrophenyl) Maltoside as a Substrate for the Assy of Amylase, Nature, 182, 525-526, 1958
JANSEN et al., alpha-(p-Nitrophenyl) Maltoside as a Substrate for the Assy of Amylase, Nature, 182, 525-526, 1958 *
Methods in Enzymology, Bd.8, S.559, 1966 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2752501A1 (de) * 1977-11-24 1979-05-31 Kurt Prof Dr Wallenfels Mit indikatorgruppen substituierte alpha-d-maltodextrine, verfahren zu deren herstellung und ihre verwendung zur bestimmung von glukosidasen und amylasen
DE2755803A1 (de) * 1977-12-14 1979-06-21 Boehringer Mannheim Gmbh Verfahren und reagens zur bestimmung von alpha-amylase

Also Published As

Publication number Publication date
IT1114891B (it) 1986-01-27
LU77751A1 (cs) 1978-02-02
DK155751B (da) 1989-05-08
GB1571642A (en) 1980-07-16
DE2731421C2 (cs) 1988-07-07
BE856737A (fr) 1978-01-12
DK315777A (da) 1978-01-14
JPS5311092A (en) 1978-02-01
NL179399B (nl) 1986-04-01
NL179399C (nl) 1986-09-01
IE45395B1 (en) 1982-08-11
FR2358660A1 (fr) 1978-02-10
FR2358660B1 (cs) 1983-08-12
NL7707682A (nl) 1978-01-17
CH633582A5 (de) 1982-12-15
IE45395L (en) 1978-01-12
JPS5753079B2 (cs) 1982-11-11
CA1104912A (en) 1981-07-14
DK155751C (da) 1989-10-23

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