DE2528617A1 - Verfahren zum immobilisieren von enzymen, die dabei erhaltenen produkte und ihre verwendung - Google Patents
Verfahren zum immobilisieren von enzymen, die dabei erhaltenen produkte und ihre verwendungInfo
- Publication number
- DE2528617A1 DE2528617A1 DE19752528617 DE2528617A DE2528617A1 DE 2528617 A1 DE2528617 A1 DE 2528617A1 DE 19752528617 DE19752528617 DE 19752528617 DE 2528617 A DE2528617 A DE 2528617A DE 2528617 A1 DE2528617 A1 DE 2528617A1
- Authority
- DE
- Germany
- Prior art keywords
- enzyme
- microporous
- volume
- hydrodynamic volume
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000004190 Enzymes Human genes 0.000 title claims description 158
- 108090000790 Enzymes Proteins 0.000 title claims description 158
- 238000000034 method Methods 0.000 title claims description 33
- 230000003100 immobilizing effect Effects 0.000 title description 7
- 239000000243 solution Substances 0.000 claims description 36
- 230000000694 effects Effects 0.000 claims description 22
- 239000011148 porous material Substances 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 239000000284 extract Substances 0.000 claims description 13
- 239000000835 fiber Substances 0.000 claims description 13
- 239000000463 material Substances 0.000 claims description 13
- 239000000126 substance Substances 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 8
- 230000007935 neutral effect Effects 0.000 claims description 6
- 239000003054 catalyst Substances 0.000 claims description 5
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 230000007423 decrease Effects 0.000 claims description 3
- WUBBRNOQWQTFEX-UHFFFAOYSA-N 4-aminosalicylic acid Chemical group NC1=CC=C(C(O)=O)C(O)=C1 WUBBRNOQWQTFEX-UHFFFAOYSA-N 0.000 claims 1
- 230000003247 decreasing effect Effects 0.000 claims 1
- 239000012442 inert solvent Substances 0.000 claims 1
- 229920005615 natural polymer Polymers 0.000 claims 1
- 230000000717 retained effect Effects 0.000 claims 1
- 239000002904 solvent Substances 0.000 claims 1
- 229920001059 synthetic polymer Polymers 0.000 claims 1
- 229940088598 enzyme Drugs 0.000 description 119
- 108010046334 Urease Proteins 0.000 description 13
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 12
- 238000006243 chemical reaction Methods 0.000 description 8
- 230000002255 enzymatic effect Effects 0.000 description 7
- 238000001914 filtration Methods 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 6
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- BJIOGJUNALELMI-ONEGZZNKSA-N Isoeugenol Natural products COC1=CC(\C=C\C)=CC=C1O BJIOGJUNALELMI-ONEGZZNKSA-N 0.000 description 5
- 229920002678 cellulose Polymers 0.000 description 5
- BJIOGJUNALELMI-ARJAWSKDSA-N cis-isoeugenol Chemical compound COC1=CC(\C=C/C)=CC=C1O BJIOGJUNALELMI-ARJAWSKDSA-N 0.000 description 5
- 150000001875 compounds Chemical class 0.000 description 5
- 239000012153 distilled water Substances 0.000 description 5
- -1 hydroxyalkyl acrylate Chemical compound 0.000 description 5
- BJIOGJUNALELMI-UHFFFAOYSA-N trans-isoeugenol Natural products COC1=CC(C=CC)=CC=C1O BJIOGJUNALELMI-UHFFFAOYSA-N 0.000 description 5
- 102000004452 Arginase Human genes 0.000 description 4
- 108700024123 Arginases Proteins 0.000 description 4
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 4
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 4
- 230000003197 catalytic effect Effects 0.000 description 4
- 239000001913 cellulose Substances 0.000 description 4
- 239000012154 double-distilled water Substances 0.000 description 4
- 238000002955 isolation Methods 0.000 description 4
- 229920000867 polyelectrolyte Polymers 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- 108010093096 Immobilized Enzymes Proteins 0.000 description 3
- 239000007983 Tris buffer Substances 0.000 description 3
- 229910021529 ammonia Inorganic materials 0.000 description 3
- 239000000969 carrier Substances 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 238000004108 freeze drying Methods 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 229920000642 polymer Polymers 0.000 description 3
- FERIUCNNQQJTOY-UHFFFAOYSA-N Butyric acid Chemical compound CCCC(O)=O FERIUCNNQQJTOY-UHFFFAOYSA-N 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 2
- 102000004316 Oxidoreductases Human genes 0.000 description 2
- 108090000854 Oxidoreductases Proteins 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 239000003463 adsorbent Substances 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 239000007979 citrate buffer Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 2
- 239000004810 polytetrafluoroethylene Substances 0.000 description 2
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- QTAPRNXNAHBUTR-PAMPIZDHSA-L (z)-4-hydroxy-4-oxobut-2-enoate;manganese(2+) Chemical compound [Mn+2].OC(=O)\C=C/C([O-])=O.OC(=O)\C=C/C([O-])=O QTAPRNXNAHBUTR-PAMPIZDHSA-L 0.000 description 1
- 102100026277 Alpha-galactosidase A Human genes 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 229920000298 Cellophane Polymers 0.000 description 1
- 101001055222 Homo sapiens Interleukin-8 Proteins 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 102000004157 Hydrolases Human genes 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 102100026236 Interleukin-8 Human genes 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- CERQOIWHTDAKMF-UHFFFAOYSA-M Methacrylate Chemical compound CC(=C)C([O-])=O CERQOIWHTDAKMF-UHFFFAOYSA-M 0.000 description 1
- BAVYZALUXZFZLV-UHFFFAOYSA-N Methylamine Chemical compound NC BAVYZALUXZFZLV-UHFFFAOYSA-N 0.000 description 1
- 241000282376 Panthera tigris Species 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 101710171263 Peroxidase 21 Proteins 0.000 description 1
- 101710171323 Peroxidase 29 Proteins 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 1
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 1
- 239000004952 Polyamide Substances 0.000 description 1
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 1
- 108091007187 Reductases Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 102000003425 Tyrosinase Human genes 0.000 description 1
- 108060008724 Tyrosinase Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 108010030291 alpha-Galactosidase Proteins 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 description 1
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 1
- 102000006995 beta-Glucosidase Human genes 0.000 description 1
- 108010047754 beta-Glucosidase Proteins 0.000 description 1
- 230000001588 bifunctional effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 229910010293 ceramic material Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000010908 decantation Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- ITDOFWOJEDZPCF-UHFFFAOYSA-N dl-Dehydrodiisoeugenol Natural products O1C=2C(OC)=CC(C=CC)=CC=2C(C)C1C1=CC=C(O)C(OC)=C1 ITDOFWOJEDZPCF-UHFFFAOYSA-N 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- ZZUFCTLCJUWOSV-UHFFFAOYSA-N furosemide Chemical compound C1=C(Cl)C(S(=O)(=O)N)=CC(C(O)=O)=C1NCC1=CC=CO1 ZZUFCTLCJUWOSV-UHFFFAOYSA-N 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 239000011147 inorganic material Substances 0.000 description 1
- 239000001573 invertase Substances 0.000 description 1
- 235000011073 invertase Nutrition 0.000 description 1
- 239000003456 ion exchange resin Substances 0.000 description 1
- 229920003303 ion-exchange polymer Polymers 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 229940040511 liver extract Drugs 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 150000002772 monosaccharides Chemical class 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 229920002647 polyamide Polymers 0.000 description 1
- 229920000515 polycarbonate Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920000728 polyester Polymers 0.000 description 1
- 229920000098 polyolefin Polymers 0.000 description 1
- ASHGTUMKRVIOLH-UHFFFAOYSA-L potassium;sodium;hydrogen phosphate Chemical compound [Na+].[K+].OP([O-])([O-])=O ASHGTUMKRVIOLH-UHFFFAOYSA-L 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000004809 thin layer chromatography Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J37/00—Processes, in general, for preparing catalysts; Processes, in general, for activation of catalysts
- B01J37/02—Impregnation, coating or precipitation
- B01J37/0201—Impregnation
- B01J37/0203—Impregnation the impregnation liquid containing organic compounds
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Dispersion Chemistry (AREA)
- Materials Engineering (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US48442574A | 1974-07-01 | 1974-07-01 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2528617A1 true DE2528617A1 (de) | 1976-01-22 |
Family
ID=23924126
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19752528617 Pending DE2528617A1 (de) | 1974-07-01 | 1975-06-26 | Verfahren zum immobilisieren von enzymen, die dabei erhaltenen produkte und ihre verwendung |
Country Status (9)
| Country | Link |
|---|---|
| JP (1) | JPS5126286A (enExample) |
| DE (1) | DE2528617A1 (enExample) |
| DK (1) | DK290775A (enExample) |
| FR (1) | FR2277098A1 (enExample) |
| GB (1) | GB1461025A (enExample) |
| IL (1) | IL47599A0 (enExample) |
| IT (1) | IT1040776B (enExample) |
| NL (1) | NL7507677A (enExample) |
| SE (1) | SE7507463L (enExample) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5536094A (en) * | 1978-09-08 | 1980-03-13 | Yoshiyo Hatano | Molding sand of good disintegrating quality |
-
1975
- 1975-06-26 DE DE19752528617 patent/DE2528617A1/de active Pending
- 1975-06-26 DK DK290775A patent/DK290775A/da unknown
- 1975-06-27 GB GB2729675A patent/GB1461025A/en not_active Expired
- 1975-06-27 NL NL7507677A patent/NL7507677A/xx unknown
- 1975-06-29 IL IL47599A patent/IL47599A0/xx unknown
- 1975-06-30 IT IT50296/75A patent/IT1040776B/it active
- 1975-06-30 SE SE7507463A patent/SE7507463L/ not_active Application Discontinuation
- 1975-06-30 FR FR7520543A patent/FR2277098A1/fr active Granted
- 1975-07-01 JP JP50081368A patent/JPS5126286A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| FR2277098B1 (enExample) | 1979-08-03 |
| GB1461025A (en) | 1977-01-13 |
| JPS5126286A (en) | 1976-03-04 |
| DK290775A (da) | 1976-01-02 |
| IT1040776B (it) | 1979-12-20 |
| SE7507463L (sv) | 1976-01-02 |
| FR2277098A1 (fr) | 1976-01-30 |
| NL7507677A (nl) | 1976-01-05 |
| IL47599A0 (en) | 1975-08-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE3012693C2 (enExample) | ||
| DE69404848T2 (de) | Filterpatrone mit darauf angeordneten unlöslichen enzympartikeln | |
| DE2915135C2 (enExample) | ||
| DE1949943A1 (de) | Stabilisiertes Enzympraeparat und Verfahren zu seiner Herstellung | |
| KR880700854A (ko) | 신규한 고정된 생체촉매와 그 제법 및 용도 | |
| AT401653B (de) | Verfahren zur immobilisierung biologischer komponenten in einer polymermatrix sowie biosensoren unter verwendung derartiger immobilisate | |
| DE2339238B2 (de) | Enzyme und/oder mikroorganismen in loesung oder dispersion enthaltender hohlfaden und verfahren zu seiner herstellung | |
| DE3132497C2 (enExample) | ||
| DE4429018C2 (de) | Verfahren zur Herstellung eines Enzyme immobilisierenden Trägers, Träger aus regeneriertem porösen Chitosan in Teilchenform und Verwendung des Trägers | |
| DE2410693A1 (de) | Verfahren zum immobilisieren von enzymen | |
| EP0207100B1 (de) | Verfahren zum ändern der wirksamen porenweite einer struktur | |
| DE2611258C2 (de) | Verfahren zum Extrahieren eines Polypeptids aus einer wäßrigen Lösung | |
| DE2528617A1 (de) | Verfahren zum immobilisieren von enzymen, die dabei erhaltenen produkte und ihre verwendung | |
| EP0097281B1 (de) | Verfahren zur Herstellung eines unlöslichen Biokatalysators | |
| US4830753A (en) | Membrane filtration of cell culture media with charged particles | |
| DE2605797C3 (de) | Verfahren zur Herstellung von immobilisierten Enzymen | |
| DE3236157C2 (enExample) | ||
| DE60306055T2 (de) | Verfahren zur Regeneration eines immobilisierten Enzyms | |
| DE3634761C1 (de) | Protoplasmareiche,grosstechnisch einsetzbare Pilzhyphen mit hoher Enzymaktivitaet und Verfahren zu ihrer Herstellung | |
| DE3636735C2 (de) | Biochemisch aktive Matrix und Verfahren zu deren Herstellung | |
| DE1907365A1 (de) | Verfahren zur Herstellung einer zellfreien Penicillinacylase | |
| DE3005632C2 (de) | Verfahren zur Herstellung von Biokatalysatoren mit hoher mechanischer Festigkeit und hoher Beladung an enzymatisch aktiver Substanz | |
| DE3005633C2 (de) | Verfahren zur Herstellung von perlförmigen Biokatalysatoren mit extrem empfindlicher enzymatisch aktiver Substanz | |
| DE3534983C2 (enExample) | ||
| DD285372A5 (de) | Verfahren zur immobilisierung von mikroorganismen bzw. coimmobilisierung von mikroorganismen und enzymen |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OHJ | Non-payment of the annual fee |