GB1461025A - Method of immobbilizing enzymes for isolation or use - Google Patents

Method of immobbilizing enzymes for isolation or use

Info

Publication number
GB1461025A
GB1461025A GB2729675A GB2729675A GB1461025A GB 1461025 A GB1461025 A GB 1461025A GB 2729675 A GB2729675 A GB 2729675A GB 2729675 A GB2729675 A GB 2729675A GB 1461025 A GB1461025 A GB 1461025A
Authority
GB
United Kingdom
Prior art keywords
enzyme
solution
carrier
hydrodynamic volume
isolation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2729675A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Research Corp
Original Assignee
Research Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Research Corp filed Critical Research Corp
Publication of GB1461025A publication Critical patent/GB1461025A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J37/00—Processes, in general, for preparing catalysts; Processes, in general, for activation of catalysts
    • B01J37/02—Impregnation, coating or precipitation
    • B01J37/0201—Impregnation
    • B01J37/0203—Impregnation the impregnation liquid containing organic compounds

Abstract

1461025 Enzymatic catalyst compositions RESEARCH CORP 27 June 1975 [1 July 1974] 27296/75 Heading C3H A method of preparing an enzymatic composition useful in heterogeneous catalysis comprises (1) reducing the hydrodynamic volume of an aqueous solution of the enzyme by adjusting the pH of the solution, (2) contacting the solution with a microporous carrier having micropores therein equal to or greater in volume than the reduced hydrodynamic volume of the enzyme so that the enzyme is free to enter within the pores of the carrier, and (3) readjusting the pH of the enzyme solution while in contact with the microporous carrier to increase the hydrodynamic volume thereof and physically trap the expanded enzyme within the pores of the microporous carrier. The method may be applied to isolation of water-soluble enzymes from their natural sources. Examples describe the preparation of urease, arginase and peroxidase compositions using cellulose pulp as support.
GB2729675A 1974-07-01 1975-06-27 Method of immobbilizing enzymes for isolation or use Expired GB1461025A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US48442574A 1974-07-01 1974-07-01

Publications (1)

Publication Number Publication Date
GB1461025A true GB1461025A (en) 1977-01-13

Family

ID=23924126

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2729675A Expired GB1461025A (en) 1974-07-01 1975-06-27 Method of immobbilizing enzymes for isolation or use

Country Status (9)

Country Link
JP (1) JPS5126286A (en)
DE (1) DE2528617A1 (en)
DK (1) DK290775A (en)
FR (1) FR2277098A1 (en)
GB (1) GB1461025A (en)
IL (1) IL47599A0 (en)
IT (1) IT1040776B (en)
NL (1) NL7507677A (en)
SE (1) SE7507463L (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5536094A (en) * 1978-09-08 1980-03-13 Yoshiyo Hatano Molding sand of good disintegrating quality

Also Published As

Publication number Publication date
NL7507677A (en) 1976-01-05
IL47599A0 (en) 1975-08-31
DE2528617A1 (en) 1976-01-22
FR2277098B1 (en) 1979-08-03
JPS5126286A (en) 1976-03-04
SE7507463L (en) 1976-01-02
DK290775A (en) 1976-01-02
IT1040776B (en) 1979-12-20
FR2277098A1 (en) 1976-01-30

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PCNP Patent ceased through non-payment of renewal fee