DE2246655C2 - Temperaturunabhängige Fällmittellösung - Google Patents
Temperaturunabhängige FällmittellösungInfo
- Publication number
- DE2246655C2 DE2246655C2 DE2246655A DE2246655A DE2246655C2 DE 2246655 C2 DE2246655 C2 DE 2246655C2 DE 2246655 A DE2246655 A DE 2246655A DE 2246655 A DE2246655 A DE 2246655A DE 2246655 C2 DE2246655 C2 DE 2246655C2
- Authority
- DE
- Germany
- Prior art keywords
- weight
- solution
- amylase
- precipitant solution
- ethylene glycol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000000243 solution Substances 0.000 claims description 44
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 21
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 claims description 20
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 claims description 18
- 239000011592 zinc chloride Substances 0.000 claims description 9
- 235000005074 zinc chloride Nutrition 0.000 claims description 9
- XNWFRZJHXBZDAG-UHFFFAOYSA-N 2-METHOXYETHANOL Chemical group COCCO XNWFRZJHXBZDAG-UHFFFAOYSA-N 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 239000005711 Benzoic acid Substances 0.000 claims description 7
- 235000010233 benzoic acid Nutrition 0.000 claims description 7
- 239000007864 aqueous solution Substances 0.000 claims description 4
- 150000008044 alkali metal hydroxides Chemical class 0.000 claims description 3
- ZNQVEEAIQZEUHB-UHFFFAOYSA-N 2-ethoxyethanol Chemical compound CCOCCO ZNQVEEAIQZEUHB-UHFFFAOYSA-N 0.000 claims description 2
- 108010065511 Amylases Proteins 0.000 description 25
- 102000013142 Amylases Human genes 0.000 description 25
- 235000019418 amylase Nutrition 0.000 description 25
- 239000004382 Amylase Substances 0.000 description 24
- LYCAIKOWRPUZTN-UHFFFAOYSA-N ethylene glycol Natural products OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 14
- 238000000034 method Methods 0.000 description 13
- 229920002472 Starch Polymers 0.000 description 11
- 235000019698 starch Nutrition 0.000 description 11
- 239000008107 starch Substances 0.000 description 11
- 239000000872 buffer Substances 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 239000012153 distilled water Substances 0.000 description 7
- 210000002966 serum Anatomy 0.000 description 7
- 229920000945 Amylopectin Polymers 0.000 description 6
- -1 chlorine ions Chemical class 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000001556 precipitation Methods 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 108090000637 alpha-Amylases Proteins 0.000 description 4
- 102000004139 alpha-Amylases Human genes 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 229920000856 Amylose Polymers 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 229940024171 alpha-amylase Drugs 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 229940088598 enzyme Drugs 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000011541 reaction mixture Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 3
- 229960001763 zinc sulfate Drugs 0.000 description 3
- 229910000368 zinc sulfate Inorganic materials 0.000 description 3
- TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 239000001263 FEMA 3042 Substances 0.000 description 2
- OWYWGLHRNBIFJP-UHFFFAOYSA-N Ipazine Chemical compound CCN(CC)C1=NC(Cl)=NC(NC(C)C)=N1 OWYWGLHRNBIFJP-UHFFFAOYSA-N 0.000 description 2
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 description 2
- 210000000683 abdominal cavity Anatomy 0.000 description 2
- 230000001476 alcoholic effect Effects 0.000 description 2
- 229940025131 amylases Drugs 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 229920002258 tannic acid Polymers 0.000 description 2
- LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 description 2
- 229940033123 tannic acid Drugs 0.000 description 2
- 235000015523 tannic acid Nutrition 0.000 description 2
- RTLULCVBFCRQKI-UHFFFAOYSA-N 1-amino-4-[3-[(4,6-dichloro-1,3,5-triazin-2-yl)amino]-4-sulfoanilino]-9,10-dioxoanthracene-2-sulfonic acid Chemical compound C1=2C(=O)C3=CC=CC=C3C(=O)C=2C(N)=C(S(O)(=O)=O)C=C1NC(C=1)=CC=C(S(O)(=O)=O)C=1NC1=NC(Cl)=NC(Cl)=N1 RTLULCVBFCRQKI-UHFFFAOYSA-N 0.000 description 1
- 208000000668 Chronic Pancreatitis Diseases 0.000 description 1
- 206010010774 Constipation Diseases 0.000 description 1
- 101100346656 Drosophila melanogaster strat gene Proteins 0.000 description 1
- 208000005647 Mumps Diseases 0.000 description 1
- 206010061902 Pancreatic neoplasm Diseases 0.000 description 1
- 206010033645 Pancreatitis Diseases 0.000 description 1
- 206010033647 Pancreatitis acute Diseases 0.000 description 1
- 206010033649 Pancreatitis chronic Diseases 0.000 description 1
- 208000007107 Stomach Ulcer Diseases 0.000 description 1
- 201000003229 acute pancreatitis Diseases 0.000 description 1
- 229910052783 alkali metal Inorganic materials 0.000 description 1
- 150000001340 alkali metals Chemical class 0.000 description 1
- 108010019077 beta-Amylase Proteins 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008366 buffered solution Substances 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 208000017169 kidney disease Diseases 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 208000015486 malignant pancreatic neoplasm Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 208000010805 mumps infectious disease Diseases 0.000 description 1
- 201000002528 pancreatic cancer Diseases 0.000 description 1
- 208000008443 pancreatic carcinoma Diseases 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 210000003079 salivary gland Anatomy 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000010414 supernatant solution Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- ZHDFKPUCUYZGGM-UHFFFAOYSA-K trisodium 4-hydroxy-3-[(2-sulfonatophenyl)diazenyl]-5-[(2,5,6-trichloropyrimidin-4-yl)amino]naphthalene-2,7-disulfonate Chemical compound [Na+].[Na+].[Na+].Oc1c(N=Nc2ccccc2S([O-])(=O)=O)c(cc2cc(cc(Nc3nc(Cl)nc(Cl)c3Cl)c12)S([O-])(=O)=O)S([O-])(=O)=O ZHDFKPUCUYZGGM-UHFFFAOYSA-K 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US20529471A | 1971-12-06 | 1971-12-06 | |
| US24139072A | 1972-04-05 | 1972-04-05 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE2246655A1 DE2246655A1 (de) | 1973-06-07 |
| DE2246655C2 true DE2246655C2 (de) | 1984-01-26 |
Family
ID=26900297
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2246655A Expired DE2246655C2 (de) | 1971-12-06 | 1972-09-22 | Temperaturunabhängige Fällmittellösung |
Country Status (11)
| Country | Link |
|---|---|
| US (2) | US3753864A (enExample) |
| JP (1) | JPS5343838B2 (enExample) |
| AU (1) | AU468613B2 (enExample) |
| CA (1) | CA953623A (enExample) |
| DE (1) | DE2246655C2 (enExample) |
| FI (1) | FI53368C (enExample) |
| FR (1) | FR2164154A5 (enExample) |
| GB (1) | GB1353572A (enExample) |
| IT (1) | IT983179B (enExample) |
| NO (1) | NO138497C (enExample) |
| SE (1) | SE403519B (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3953297A (en) * | 1973-02-26 | 1976-04-27 | Pierce Chemical Company | Determination of amylase |
| US4321364A (en) * | 1980-04-17 | 1982-03-23 | Minister For Public Works For The State Of New South Wales | Preparation of soluble chromogenic substrates |
| US4963479A (en) * | 1986-10-07 | 1990-10-16 | Hoechst Celanese Corporation | Reagent system for an alpha-amylase assay containing aromatic substituted glycoside |
| US5158872A (en) * | 1986-10-07 | 1992-10-27 | Hoechst Celanese Corporation | Aromatic substituted glycoside |
-
1971
- 1971-12-06 US US00205294A patent/US3753864A/en not_active Expired - Lifetime
-
1972
- 1972-04-05 US US00241390A patent/US3758384A/en not_active Expired - Lifetime
- 1972-09-22 DE DE2246655A patent/DE2246655C2/de not_active Expired
- 1972-10-26 FI FI2972/72A patent/FI53368C/fi active
- 1972-10-30 JP JP10802672A patent/JPS5343838B2/ja not_active Expired
- 1972-11-01 AU AU48411/72A patent/AU468613B2/en not_active Expired
- 1972-11-10 GB GB5193372A patent/GB1353572A/en not_active Expired
- 1972-11-14 FR FR7240280A patent/FR2164154A5/fr not_active Expired
- 1972-11-21 CA CA157,130A patent/CA953623A/en not_active Expired
- 1972-12-05 NO NO4468/72A patent/NO138497C/no unknown
- 1972-12-05 SE SE7215860A patent/SE403519B/xx unknown
- 1972-12-06 IT IT32536/72A patent/IT983179B/it active
Also Published As
| Publication number | Publication date |
|---|---|
| DE2246655A1 (de) | 1973-06-07 |
| FI53368C (fi) | 1978-04-10 |
| US3758384A (en) | 1973-09-11 |
| IT983179B (it) | 1974-10-31 |
| AU4841172A (en) | 1974-05-02 |
| JPS5343838B2 (enExample) | 1978-11-22 |
| JPS4865996A (enExample) | 1973-09-10 |
| NO138497C (no) | 1978-09-13 |
| CA953623A (en) | 1974-08-27 |
| US3753864A (en) | 1973-08-21 |
| NO138497B (no) | 1978-06-05 |
| FI53368B (enExample) | 1977-12-30 |
| SE403519B (sv) | 1978-08-21 |
| AU468613B2 (en) | 1976-01-15 |
| GB1353572A (en) | 1974-05-22 |
| FR2164154A5 (enExample) | 1973-07-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0035211B1 (de) | Verfahren und Reagens zur Bestimmung der beta-Lipoproteine (LDL) | |
| EP0201805A1 (de) | Stabilisiertes Sarcosinoxidase-Präparat | |
| McAllister et al. | Heparin metabolism: isolation and characterization of uroheparin | |
| DE2246655C2 (de) | Temperaturunabhängige Fällmittellösung | |
| DE1643196C2 (de) | Wasserlösliche aus Stärke, Amylose oder Amylopektin und einem Farbstoff bestehende Verbindungen sowie Verfahren zu deren Herstellung | |
| DE2751904C2 (de) | Verfahren und Mittel zur Bestimmung von Kreatinin in biologischen Flüssigkeiten | |
| CH640003A5 (de) | Verfahren zur bestimmung von alpha-amylase. | |
| DE2335350C2 (de) | Bestimmung von Calcium | |
| EP0034692B1 (de) | Die Verwendung von Stärkederivaten für Indikatormittel | |
| DE2256331A1 (de) | Verfahren zur harnsaeurebestimmung | |
| DE2032270A1 (de) | Diagnostische Reagenzien für die Bestimmung von gamma-Glutamyltranspeptidase im menschlichen Serum | |
| DE2748857A1 (de) | Verfahren zur quantitativen bestimmung von harnstoff | |
| DE1940869B2 (de) | Amylaseaktivitat | |
| EP0049849B1 (de) | Verfahren zur Bestimmung der Streptokokken-Desoxiribonuclease B nach der Toluidinblau O-Methode | |
| DE69030135T2 (de) | Ein Reagenssystem zur Bestimmung von Lipase | |
| DE10144436B4 (de) | Testformulierung und ihre Verwendung zur gleichzeitigen Bestimmung von Gesamteiweiß und Harnsäure in biologischen Flüssigkeiten | |
| EP0351605B1 (de) | Quantitative Bestimmung von Phosphor | |
| DE3854127T2 (de) | Stabiles alpha-amylase-reagenz. | |
| EP0017909B1 (de) | Verfahren zur Bestimmung von Anti-Hyaluronidase und hierfür verwendbares Mittel | |
| DE2123032A1 (de) | Verfahren zur Bestimmung von Harnsäure | |
| DE3889845T2 (de) | Verfahren zur Massanalyse von Wasserstoff-Peroxyd und Reagens dafür. | |
| DE3020072A1 (de) | Mittel und verfahren zur bestimmung von lipase | |
| US3511611A (en) | Blood urea nitrogen test | |
| DE69023710T2 (de) | Verfahren zur bestimmung von enzymatischer wirksamkeit. | |
| Li | Electrophoresis of Rat Sera1 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OD | Request for examination | ||
| 8128 | New person/name/address of the agent |
Representative=s name: HENKEL, G., DR.PHIL. FEILER, L., DR.RER.NAT., PAT. |
|
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition | ||
| 8339 | Ceased/non-payment of the annual fee |