DE2224336A1 - Erzeugung von Metaboliten durch Synthese in Pflanzenzellen - Google Patents
Erzeugung von Metaboliten durch Synthese in PflanzenzellenInfo
- Publication number
- DE2224336A1 DE2224336A1 DE19722224336 DE2224336A DE2224336A1 DE 2224336 A1 DE2224336 A1 DE 2224336A1 DE 19722224336 DE19722224336 DE 19722224336 DE 2224336 A DE2224336 A DE 2224336A DE 2224336 A1 DE2224336 A1 DE 2224336A1
- Authority
- DE
- Germany
- Prior art keywords
- medium
- plant
- undifferentiated
- growth
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002207 metabolite Substances 0.000 title claims description 33
- 238000004519 manufacturing process Methods 0.000 title claims description 11
- 230000015572 biosynthetic process Effects 0.000 title description 13
- 238000003786 synthesis reaction Methods 0.000 title description 2
- 239000002609 medium Substances 0.000 claims description 69
- 230000012010 growth Effects 0.000 claims description 65
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 claims description 61
- 229930192334 Auxin Natural products 0.000 claims description 43
- 239000002363 auxin Substances 0.000 claims description 43
- 230000000694 effects Effects 0.000 claims description 37
- 239000000126 substance Substances 0.000 claims description 23
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 claims description 22
- HXKWSTRRCHTUEC-UHFFFAOYSA-N 2,4-Dichlorophenoxyaceticacid Chemical compound OC(=O)C(Cl)OC1=CC=C(Cl)C=C1 HXKWSTRRCHTUEC-UHFFFAOYSA-N 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 17
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 14
- 150000001875 compounds Chemical class 0.000 claims description 13
- 238000004114 suspension culture Methods 0.000 claims description 12
- 235000015097 nutrients Nutrition 0.000 claims description 11
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- 229940088594 vitamin Drugs 0.000 claims description 11
- 229930003231 vitamin Natural products 0.000 claims description 11
- 235000013343 vitamin Nutrition 0.000 claims description 11
- 239000011782 vitamin Substances 0.000 claims description 11
- 239000005971 1-naphthylacetic acid Substances 0.000 claims description 8
- IIDAJRNSZSFFCB-UHFFFAOYSA-N 4-amino-5-methoxy-2-methylbenzenesulfonamide Chemical compound COC1=CC(S(N)(=O)=O)=C(C)C=C1N IIDAJRNSZSFFCB-UHFFFAOYSA-N 0.000 claims description 8
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 7
- 229910052799 carbon Inorganic materials 0.000 claims description 7
- 229910052757 nitrogen Inorganic materials 0.000 claims description 7
- 239000012737 fresh medium Substances 0.000 claims description 6
- SMYMJHWAQXWPDB-UHFFFAOYSA-N (2,4,5-trichlorophenoxy)acetic acid Chemical compound OC(=O)COC1=CC(Cl)=C(Cl)C=C1Cl SMYMJHWAQXWPDB-UHFFFAOYSA-N 0.000 claims description 3
- 239000003559 2,4,5-trichlorophenoxyacetic acid Substances 0.000 claims description 3
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 3
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 3
- 230000003247 decreasing effect Effects 0.000 claims description 3
- 239000011777 magnesium Substances 0.000 claims description 3
- 229910052749 magnesium Inorganic materials 0.000 claims description 3
- 239000011574 phosphorus Substances 0.000 claims description 3
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- 230000008569 process Effects 0.000 claims description 3
- SODPIMGUZLOIPE-UHFFFAOYSA-N (4-chlorophenoxy)acetic acid Chemical compound OC(=O)COC1=CC=C(Cl)C=C1 SODPIMGUZLOIPE-UHFFFAOYSA-N 0.000 claims description 2
- 238000009825 accumulation Methods 0.000 claims description 2
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- 239000005708 Sodium hypochlorite Substances 0.000 description 9
- 229930006000 Sucrose Natural products 0.000 description 9
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 9
- 239000002054 inoculum Substances 0.000 description 9
- -1 nitrate ions Chemical class 0.000 description 9
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 9
- 239000005720 sucrose Substances 0.000 description 9
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- UHOVQNZJYSORNB-UHFFFAOYSA-N Benzene Chemical compound C1=CC=CC=C1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 6
- 241000335053 Beta vulgaris Species 0.000 description 6
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- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 6
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- 238000002474 experimental method Methods 0.000 description 6
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- 244000165082 Lavanda vera Species 0.000 description 5
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 5
- 150000003797 alkaloid derivatives Chemical class 0.000 description 5
- 239000004327 boric acid Substances 0.000 description 5
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 5
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- OTYVBQZXUNBRTK-UHFFFAOYSA-N 3,3,6-trimethylhepta-1,5-dien-4-one Chemical compound CC(C)=CC(=O)C(C)(C)C=C OTYVBQZXUNBRTK-UHFFFAOYSA-N 0.000 description 4
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- 150000002484 inorganic compounds Chemical class 0.000 description 1
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- 238000005342 ion exchange Methods 0.000 description 1
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- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 239000000171 lavandula angustifolia l. flower oil Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- VUABZDVEQNHNTD-UHFFFAOYSA-K magnesium;potassium;dihydrogen phosphate;sulfate;heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[K+].OP(O)([O-])=O.[O-]S([O-])(=O)=O VUABZDVEQNHNTD-UHFFFAOYSA-K 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- ISPYRSDWRDQNSW-UHFFFAOYSA-L manganese(II) sulfate monohydrate Chemical compound O.[Mn+2].[O-]S([O-])(=O)=O ISPYRSDWRDQNSW-UHFFFAOYSA-L 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 238000001819 mass spectrum Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 229920000609 methyl cellulose Polymers 0.000 description 1
- WBYWAXJHAXSJNI-UHFFFAOYSA-N methyl p-hydroxycinnamate Natural products OC(=O)C=CC1=CC=CC=C1 WBYWAXJHAXSJNI-UHFFFAOYSA-N 0.000 description 1
- 239000001923 methylcellulose Substances 0.000 description 1
- 235000010981 methylcellulose Nutrition 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 239000011785 micronutrient Substances 0.000 description 1
- 235000013369 micronutrients Nutrition 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 238000013206 minimal dilution Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 229960002715 nicotine Drugs 0.000 description 1
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 235000011837 pasties Nutrition 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 229930195732 phytohormone Natural products 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- CLSUSRZJUQMOHH-UHFFFAOYSA-L platinum dichloride Chemical compound Cl[Pt]Cl CLSUSRZJUQMOHH-UHFFFAOYSA-L 0.000 description 1
- 229920001467 poly(styrenesulfonates) Polymers 0.000 description 1
- 239000001103 potassium chloride Substances 0.000 description 1
- 235000011164 potassium chloride Nutrition 0.000 description 1
- 229910000160 potassium phosphate Inorganic materials 0.000 description 1
- 235000011009 potassium phosphates Nutrition 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 229940080360 rauwolfia alkaloid Drugs 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
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- JUVIOZPCNVVQFO-HBGVWJBISA-N rotenone Chemical compound O([C@H](CC1=C2O3)C(C)=C)C1=CC=C2C(=O)[C@@H]1[C@H]3COC2=C1C=C(OC)C(OC)=C2 JUVIOZPCNVVQFO-HBGVWJBISA-N 0.000 description 1
- JUVIOZPCNVVQFO-UHFFFAOYSA-N rotenone Natural products O1C2=C3CC(C(C)=C)OC3=CC=C2C(=O)C2C1COC1=C2C=C(OC)C(OC)=C1 JUVIOZPCNVVQFO-UHFFFAOYSA-N 0.000 description 1
- 239000010672 sassafras oil Substances 0.000 description 1
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- 238000005204 segregation Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012807 shake-flask culturing Methods 0.000 description 1
- 231100000161 signs of toxicity Toxicity 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- BBMHARZCALWXSL-UHFFFAOYSA-M sodium dihydrogenphosphate monohydrate Chemical compound O.[Na+].OP(O)([O-])=O BBMHARZCALWXSL-UHFFFAOYSA-M 0.000 description 1
- 239000011684 sodium molybdate Substances 0.000 description 1
- 235000015393 sodium molybdate Nutrition 0.000 description 1
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000011343 solid material Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 150000008143 steroidal glycosides Chemical class 0.000 description 1
- 239000000021 stimulant Substances 0.000 description 1
- 229960005453 strychnine Drugs 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 150000003467 sulfuric acid derivatives Chemical class 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 239000006188 syrup Substances 0.000 description 1
- 235000020357 syrup Nutrition 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 229960000344 thiamine hydrochloride Drugs 0.000 description 1
- 235000019190 thiamine hydrochloride Nutrition 0.000 description 1
- 239000011747 thiamine hydrochloride Substances 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 1
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- MWOOGOJBHIARFG-UHFFFAOYSA-N vanillin Chemical compound COC1=CC(C=O)=CC=C1O MWOOGOJBHIARFG-UHFFFAOYSA-N 0.000 description 1
- FGQOOHJZONJGDT-UHFFFAOYSA-N vanillin Natural products COC1=CC(O)=CC(C=O)=C1 FGQOOHJZONJGDT-UHFFFAOYSA-N 0.000 description 1
- 235000012141 vanillin Nutrition 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 235000020047 vermouth Nutrition 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- OGWKCGZFUXNPDA-XQKSVPLYSA-N vincristine Chemical compound C([N@]1C[C@@H](C[C@]2(C(=O)OC)C=3C(=CC4=C([C@]56[C@H]([C@@]([C@H](OC(C)=O)[C@]7(CC)C=CCN([C@H]67)CC5)(O)C(=O)OC)N4C=O)C=3)OC)C[C@@](C1)(O)CC)CC1=C2NC2=CC=CC=C12 OGWKCGZFUXNPDA-XQKSVPLYSA-N 0.000 description 1
- 229960004528 vincristine Drugs 0.000 description 1
- OGWKCGZFUXNPDA-UHFFFAOYSA-N vincristine Natural products C1C(CC)(O)CC(CC2(C(=O)OC)C=3C(=CC4=C(C56C(C(C(OC(C)=O)C7(CC)C=CCN(C67)CC5)(O)C(=O)OC)N4C=O)C=3)OC)CN1CCC1=C2NC2=CC=CC=C12 OGWKCGZFUXNPDA-UHFFFAOYSA-N 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 235000014101 wine Nutrition 0.000 description 1
- 238000010626 work up procedure Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
- 229940023877 zeatin Drugs 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1562671A GB1387821A (en) | 1971-05-18 | 1971-05-18 | Producing chemical plant metabolites by suspension culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2224336A1 true DE2224336A1 (de) | 1972-11-30 |
Family
ID=10062549
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19722224336 Pending DE2224336A1 (de) | 1971-05-18 | 1972-05-18 | Erzeugung von Metaboliten durch Synthese in Pflanzenzellen |
Country Status (17)
| Country | Link |
|---|---|
| AR (1) | AR194592A1 (cg-RX-API-DMAC10.html) |
| AU (1) | AU4191772A (cg-RX-API-DMAC10.html) |
| BE (1) | BE783584A (cg-RX-API-DMAC10.html) |
| BR (1) | BR7203127D0 (cg-RX-API-DMAC10.html) |
| CS (1) | CS158726B2 (cg-RX-API-DMAC10.html) |
| DE (1) | DE2224336A1 (cg-RX-API-DMAC10.html) |
| DK (1) | DK132990C (cg-RX-API-DMAC10.html) |
| ES (1) | ES402916A1 (cg-RX-API-DMAC10.html) |
| FR (1) | FR2138121B1 (cg-RX-API-DMAC10.html) |
| GB (1) | GB1387821A (cg-RX-API-DMAC10.html) |
| HU (1) | HU165478B (cg-RX-API-DMAC10.html) |
| IL (1) | IL39353A (cg-RX-API-DMAC10.html) |
| NL (1) | NL7206585A (cg-RX-API-DMAC10.html) |
| PH (1) | PH9403A (cg-RX-API-DMAC10.html) |
| RO (1) | RO68388A (cg-RX-API-DMAC10.html) |
| SE (1) | SE7206472L (cg-RX-API-DMAC10.html) |
| ZA (1) | ZA722917B (cg-RX-API-DMAC10.html) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1981000113A1 (en) * | 1979-06-27 | 1981-01-22 | P Brodelius | Immobilized catalysts having their origin in higher plants |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE817857A (fr) * | 1974-07-19 | 1974-11-18 | Procede de multiplication acceleree de plants de fraisiers par "micropropagation in vitro" et plantules obtenues. | |
| FR2364611A1 (fr) * | 1976-09-21 | 1978-04-14 | Anvar | Procede de multiplication vegetative de vegetaux et plants ainsi obtenus |
| US4038778A (en) * | 1976-09-27 | 1977-08-02 | Gte Laboratories Incorporated | Tissue culture technique for asexual plant reproduction and a medium for use therewith |
| US4241536A (en) * | 1976-11-10 | 1980-12-30 | Saint Firmin Annette R | Embryogenesis in vitro, induction of qualitative and quantitative changes in metabolites produced by plants and products thereof |
| US4060933A (en) * | 1976-12-20 | 1977-12-06 | Gte Laboratories Incorporated | Tissue culture technique utilizing a specific light source |
| EP0007244B1 (en) * | 1978-07-19 | 1983-05-11 | Gallaher Limited | Preparing nicotine by culturing a nicotiana strain |
| US4204366A (en) * | 1978-10-13 | 1980-05-27 | Purdue Research Foundation | Method of non-agricultural production of cotyledons |
| US4326358A (en) | 1980-07-17 | 1982-04-27 | Agrigenetics Research Associates Limited | Hybrids |
| US4381624A (en) | 1980-07-17 | 1983-05-03 | Agrigenetics Research Associates Limited | High purity hybrid cabbage seed production |
| US4426808A (en) | 1981-05-22 | 1984-01-24 | Purdue Research Foundation | Method of non-agricultural production of jojoba wax |
| AU2622988A (en) * | 1987-11-24 | 1989-06-14 | Zsuzsa Abraham | Preparation and use of plants of improved biological value |
| ES2040174B1 (es) * | 1992-02-24 | 1994-05-01 | Consejo Superior Investigacion | Metodo para el enraizamiento de estaquillas semileñosas de chirimoyo (anona cherimola mill.) cv. fino de jete, micropropagadas por cultivo de tejidos in vitro. |
| ATE354272T1 (de) * | 1999-09-21 | 2007-03-15 | Woody Plant Biotech Aps | Verfahren zum reifen von somatischen koniferenembryonen |
| US6897065B1 (en) | 1999-09-21 | 2005-05-24 | Woody Plant Biotech Aps | Method for maturation of conifer somatic embryos |
| CN111066504B (zh) * | 2019-12-04 | 2021-09-14 | 广州花卉研究中心 | 一种抑制‘粉冠军红掌’丛生苗发生的方法 |
| CN114258811B (zh) * | 2021-02-05 | 2023-03-14 | 中国林业科学研究院热带林业研究所 | 一种利用高压气体诱导土沉香结香的方法及其应用 |
-
1971
- 1971-05-18 GB GB1562671A patent/GB1387821A/en not_active Expired
-
1972
- 1972-05-01 ZA ZA722917A patent/ZA722917B/xx unknown
- 1972-05-03 IL IL39353A patent/IL39353A/xx unknown
- 1972-05-04 AU AU41917/72A patent/AU4191772A/en not_active Expired
- 1972-05-05 PH PH13520*UA patent/PH9403A/en unknown
- 1972-05-10 AR AR241904A patent/AR194592A1/es active
- 1972-05-16 NL NL7206585A patent/NL7206585A/xx unknown
- 1972-05-17 BR BR3127/72A patent/BR7203127D0/pt unknown
- 1972-05-17 BE BE783584A patent/BE783584A/xx unknown
- 1972-05-17 SE SE7206472A patent/SE7206472L/sv unknown
- 1972-05-17 DK DK247172A patent/DK132990C/da active
- 1972-05-18 FR FR7217923A patent/FR2138121B1/fr not_active Expired
- 1972-05-18 RO RO7270946A patent/RO68388A/ro unknown
- 1972-05-18 DE DE19722224336 patent/DE2224336A1/de active Pending
- 1972-05-18 ES ES402916A patent/ES402916A1/es not_active Expired
- 1972-05-18 CS CS341372A patent/CS158726B2/cs unknown
- 1972-05-18 HU HUAE359A patent/HU165478B/hu unknown
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1981000113A1 (en) * | 1979-06-27 | 1981-01-22 | P Brodelius | Immobilized catalysts having their origin in higher plants |
Also Published As
| Publication number | Publication date |
|---|---|
| SU440814A3 (cg-RX-API-DMAC10.html) | 1974-08-25 |
| DK132990C (da) | 1976-08-02 |
| NL7206585A (cg-RX-API-DMAC10.html) | 1972-11-21 |
| ZA722917B (en) | 1973-02-28 |
| BR7203127D0 (pt) | 1973-05-24 |
| IL39353A0 (en) | 1972-07-26 |
| FR2138121A1 (cg-RX-API-DMAC10.html) | 1972-12-29 |
| FR2138121B1 (cg-RX-API-DMAC10.html) | 1977-08-26 |
| BE783584A (fr) | 1972-11-17 |
| GB1387821A (en) | 1975-03-19 |
| AU4191772A (en) | 1973-11-08 |
| PH9403A (en) | 1975-11-03 |
| ES402916A1 (es) | 1975-11-01 |
| HU165478B (cg-RX-API-DMAC10.html) | 1974-09-28 |
| IL39353A (en) | 1975-04-25 |
| SE7206472L (sv) | 1972-11-20 |
| AR194592A1 (es) | 1973-07-31 |
| RO68388A (ro) | 1982-04-12 |
| CS158726B2 (cg-RX-API-DMAC10.html) | 1974-11-25 |
| DK132990B (da) | 1976-03-08 |
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| OHJ | Non-payment of the annual fee |