DE2154672A1 - Feste Komplexe von Enzymen und Verfahren zu ihrer Herstellung - Google Patents
Feste Komplexe von Enzymen und Verfahren zu ihrer HerstellungInfo
- Publication number
- DE2154672A1 DE2154672A1 DE19712154672 DE2154672A DE2154672A1 DE 2154672 A1 DE2154672 A1 DE 2154672A1 DE 19712154672 DE19712154672 DE 19712154672 DE 2154672 A DE2154672 A DE 2154672A DE 2154672 A1 DE2154672 A1 DE 2154672A1
- Authority
- DE
- Germany
- Prior art keywords
- enzyme
- clay
- invertase
- bentonite
- binder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000004190 Enzymes Human genes 0.000 title claims description 54
- 108090000790 Enzymes Proteins 0.000 title claims description 54
- 238000000034 method Methods 0.000 title claims description 17
- 239000007787 solid Substances 0.000 title claims description 11
- 238000002360 preparation method Methods 0.000 title description 11
- 108010051210 beta-Fructofuranosidase Proteins 0.000 claims description 55
- 239000001573 invertase Substances 0.000 claims description 55
- 235000011073 invertase Nutrition 0.000 claims description 55
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical group O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 52
- 239000000440 bentonite Substances 0.000 claims description 28
- 229910000278 bentonite Inorganic materials 0.000 claims description 28
- 239000000243 solution Substances 0.000 claims description 28
- 239000004927 clay Substances 0.000 claims description 25
- MGNCLNQXLYJVJD-UHFFFAOYSA-N cyanuric chloride Chemical compound ClC1=NC(Cl)=NC(Cl)=N1 MGNCLNQXLYJVJD-UHFFFAOYSA-N 0.000 claims description 20
- 239000011230 binding agent Substances 0.000 claims description 15
- 108090000604 Hydrolases Proteins 0.000 claims description 10
- 150000004820 halides Chemical class 0.000 claims description 6
- 238000006911 enzymatic reaction Methods 0.000 claims description 4
- 239000011541 reaction mixture Substances 0.000 claims description 4
- 239000007853 buffer solution Substances 0.000 claims description 3
- -1 cyanide halide Chemical class 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- QPJDMGCKMHUXFD-UHFFFAOYSA-N cyanogen chloride Chemical compound ClC#N QPJDMGCKMHUXFD-UHFFFAOYSA-N 0.000 claims description 2
- 239000000758 substrate Substances 0.000 claims description 2
- 239000012062 aqueous buffer Substances 0.000 claims 2
- 241000607479 Yersinia pestis Species 0.000 claims 1
- 230000004048 modification Effects 0.000 claims 1
- 238000012986 modification Methods 0.000 claims 1
- 239000003960 organic solvent Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 description 30
- 229940088598 enzyme Drugs 0.000 description 30
- 238000005406 washing Methods 0.000 description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
- 230000002255 enzymatic effect Effects 0.000 description 12
- 230000000977 initiatory effect Effects 0.000 description 9
- 239000008351 acetate buffer Substances 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 8
- 239000012153 distilled water Substances 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 8
- 102000004157 Hydrolases Human genes 0.000 description 6
- 238000003860 storage Methods 0.000 description 6
- 235000000346 sugar Nutrition 0.000 description 6
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 5
- 239000000969 carrier Substances 0.000 description 5
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- 150000008163 sugars Chemical class 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- GHCZTIFQWKKGSB-UHFFFAOYSA-N 2-hydroxypropane-1,2,3-tricarboxylic acid;phosphoric acid Chemical compound OP(O)(O)=O.OC(=O)CC(O)(C(O)=O)CC(O)=O GHCZTIFQWKKGSB-UHFFFAOYSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 102000035195 Peptidases Human genes 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- VZGDMQKNWNREIO-UHFFFAOYSA-N tetrachloromethane Chemical compound ClC(Cl)(Cl)Cl VZGDMQKNWNREIO-UHFFFAOYSA-N 0.000 description 2
- JVIPLYCGEZUBIO-UHFFFAOYSA-N 2-(4-fluorophenyl)-1,3-dioxoisoindole-5-carboxylic acid Chemical compound O=C1C2=CC(C(=O)O)=CC=C2C(=O)N1C1=CC=C(F)C=C1 JVIPLYCGEZUBIO-UHFFFAOYSA-N 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108090000317 Chymotrypsin Proteins 0.000 description 1
- 229920001425 Diethylaminoethyl cellulose Polymers 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- CERQOIWHTDAKMF-UHFFFAOYSA-N Methacrylic acid Chemical compound CC(=C)C(O)=O CERQOIWHTDAKMF-UHFFFAOYSA-N 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 102000016679 alpha-Glucosidases Human genes 0.000 description 1
- 108010028144 alpha-Glucosidases Proteins 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229960002376 chymotrypsin Drugs 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000008094 contradictory effect Effects 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical compound O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 150000002561 ketenes Chemical class 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 229920003145 methacrylic acid copolymer Polymers 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 229910052901 montmorillonite Inorganic materials 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 235000019833 protease Nutrition 0.000 description 1
- 230000022558 protein metabolic process Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000000932 sedative agent Substances 0.000 description 1
- 230000001624 sedative effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000009958 sewing Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 125000000185 sucrose group Chemical group 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
Landscapes
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Inorganic Chemistry (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR7039544A FR2112634A5 (enExample) | 1970-11-03 | 1970-11-03 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE2154672A1 true DE2154672A1 (de) | 1972-05-10 |
Family
ID=9063694
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19712154672 Pending DE2154672A1 (de) | 1970-11-03 | 1971-11-03 | Feste Komplexe von Enzymen und Verfahren zu ihrer Herstellung |
Country Status (8)
| Country | Link |
|---|---|
| BE (1) | BE774832A (enExample) |
| CA (1) | CA995607A (enExample) |
| DE (1) | DE2154672A1 (enExample) |
| FR (1) | FR2112634A5 (enExample) |
| GB (1) | GB1363526A (enExample) |
| IT (1) | IT946038B (enExample) |
| LU (1) | LU64188A1 (enExample) |
| NL (1) | NL7115167A (enExample) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102007027195A1 (de) | 2007-06-13 | 2008-12-18 | Süd-Chemie AG | Thermisch modifizierte Tonmineralien als Trägermaterialien für Enzyme |
| DE102007027206A1 (de) | 2007-06-13 | 2008-12-18 | Süd-Chemie AG | Immobilisierung von Enzymen auf Bleicherden |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7348182B2 (en) * | 2000-10-03 | 2008-03-25 | Mirari Biosciences, Inc. | Directed microwave chemistry |
| EP1330532B1 (en) * | 2000-10-03 | 2011-12-14 | Mirari Biosciences, Inc. | Methods and compositions for directed microwave chemistry |
| US20040209303A1 (en) | 2000-10-03 | 2004-10-21 | Martin Mark T. | Methods and compositions for directed microwave chemistry |
-
1970
- 1970-11-03 FR FR7039544A patent/FR2112634A5/fr not_active Expired
-
1971
- 1971-11-01 GB GB5067171A patent/GB1363526A/en not_active Expired
- 1971-11-02 CA CA126,721A patent/CA995607A/fr not_active Expired
- 1971-11-03 IT IT3066471A patent/IT946038B/it active
- 1971-11-03 DE DE19712154672 patent/DE2154672A1/de active Pending
- 1971-11-03 BE BE774832A patent/BE774832A/xx unknown
- 1971-11-03 LU LU64188D patent/LU64188A1/xx unknown
- 1971-11-03 NL NL7115167A patent/NL7115167A/xx unknown
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102007027195A1 (de) | 2007-06-13 | 2008-12-18 | Süd-Chemie AG | Thermisch modifizierte Tonmineralien als Trägermaterialien für Enzyme |
| DE102007027206A1 (de) | 2007-06-13 | 2008-12-18 | Süd-Chemie AG | Immobilisierung von Enzymen auf Bleicherden |
Also Published As
| Publication number | Publication date |
|---|---|
| LU64188A1 (enExample) | 1972-05-17 |
| IT946038B (it) | 1973-05-21 |
| NL7115167A (enExample) | 1972-05-05 |
| FR2112634A5 (enExample) | 1972-06-23 |
| CA995607A (fr) | 1976-08-24 |
| GB1363526A (en) | 1974-08-14 |
| BE774832A (fr) | 1972-03-01 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| OGA | New person/name/address of the applicant | ||
| OHA | Expiration of time for request for examination |