DE2131139C2 - Quervernetzte, perlförmig ausgebildete Agar- oder Agarose-Derivate, Verfahren zu ihrer Herstellung und ihre Verwendung - Google Patents
Quervernetzte, perlförmig ausgebildete Agar- oder Agarose-Derivate, Verfahren zu ihrer Herstellung und ihre VerwendungInfo
- Publication number
- DE2131139C2 DE2131139C2 DE2131139A DE2131139A DE2131139C2 DE 2131139 C2 DE2131139 C2 DE 2131139C2 DE 2131139 A DE2131139 A DE 2131139A DE 2131139 A DE2131139 A DE 2131139A DE 2131139 C2 DE2131139 C2 DE 2131139C2
- Authority
- DE
- Germany
- Prior art keywords
- agar
- gel
- cross
- agarose
- linked
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
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- 239000008272 agar Substances 0.000 title claims description 96
- 229920000936 Agarose Polymers 0.000 title claims description 33
- 238000000034 method Methods 0.000 title claims description 26
- 230000008569 process Effects 0.000 title claims description 12
- 238000004519 manufacturing process Methods 0.000 title claims description 9
- 239000000047 product Substances 0.000 claims description 44
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 44
- 239000000126 substance Substances 0.000 claims description 25
- 239000002245 particle Substances 0.000 claims description 20
- BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 claims description 14
- 230000009467 reduction Effects 0.000 claims description 10
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims description 9
- 239000003513 alkali Substances 0.000 claims description 9
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 8
- 239000012279 sodium borohydride Substances 0.000 claims description 8
- 229910000033 sodium borohydride Inorganic materials 0.000 claims description 8
- 239000007795 chemical reaction product Substances 0.000 claims description 6
- 239000011049 pearl Substances 0.000 claims description 6
- DEWLEGDTCGBNGU-UHFFFAOYSA-N 1,3-dichloropropan-2-ol Chemical compound ClCC(O)CCl DEWLEGDTCGBNGU-UHFFFAOYSA-N 0.000 claims description 5
- -1 bisepoxides Chemical compound 0.000 claims description 5
- 239000003638 chemical reducing agent Substances 0.000 claims description 5
- GKIPXFAANLTWBM-UHFFFAOYSA-N epibromohydrin Chemical compound BrCC1CO1 GKIPXFAANLTWBM-UHFFFAOYSA-N 0.000 claims description 5
- 238000005904 alkaline hydrolysis reaction Methods 0.000 claims description 4
- AFOSIXZFDONLBT-UHFFFAOYSA-N divinyl sulfone Chemical compound C=CS(=O)(=O)C=C AFOSIXZFDONLBT-UHFFFAOYSA-N 0.000 claims description 4
- 239000003960 organic solvent Substances 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 239000003431 cross linking reagent Substances 0.000 claims description 3
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- 239000001301 oxygen Substances 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 230000035484 reaction time Effects 0.000 claims description 2
- 239000002002 slurry Substances 0.000 claims description 2
- ZXCYIJGIGSDJQQ-UHFFFAOYSA-N 2,3-dichloropropan-1-ol Chemical compound OCC(Cl)CCl ZXCYIJGIGSDJQQ-UHFFFAOYSA-N 0.000 claims 1
- 241000766754 Agra Species 0.000 claims 1
- 239000000499 gel Substances 0.000 description 59
- 235000018102 proteins Nutrition 0.000 description 35
- 102000004169 proteins and genes Human genes 0.000 description 35
- 108090000623 proteins and genes Proteins 0.000 description 35
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 30
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- ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 6
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- JROOCDXTPKCUIO-UHFFFAOYSA-N 2-[4-(oxiran-2-ylmethoxy)butan-2-yloxymethyl]oxirane Chemical compound C1OC1COC(C)CCOCC1CO1 JROOCDXTPKCUIO-UHFFFAOYSA-N 0.000 description 3
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 3
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- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 2
- 239000005695 Ammonium acetate Substances 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
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- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 2
- 101710177166 Phosphoprotein Proteins 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- 239000004280 Sodium formate Substances 0.000 description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
- 239000012670 alkaline solution Substances 0.000 description 2
- 229910052782 aluminium Inorganic materials 0.000 description 2
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- 235000019257 ammonium acetate Nutrition 0.000 description 2
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- DURPTKYDGMDSBL-UHFFFAOYSA-N 1-butoxybutane Chemical compound CCCCOCCCC DURPTKYDGMDSBL-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G59/00—Polycondensates containing more than one epoxy group per molecule; Macromolecules obtained by polymerising compounds containing more than one epoxy group per molecule using curing agents or catalysts which react with the epoxy groups
- C08G59/18—Macromolecules obtained by polymerising compounds containing more than one epoxy group per molecule using curing agents or catalysts which react with the epoxy groups ; e.g. general methods of curing
- C08G59/40—Macromolecules obtained by polymerising compounds containing more than one epoxy group per molecule using curing agents or catalysts which react with the epoxy groups ; e.g. general methods of curing characterised by the curing agents used
- C08G59/62—Alcohols or phenols
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0036—Galactans; Derivatives thereof
- C08B37/0039—Agar; Agarose, i.e. D-galactose, 3,6-anhydro-D-galactose, methylated, sulfated, e.g. from the red algae Gelidium and Gracilaria; Agaropectin; Derivatives thereof, e.g. Sepharose, i.e. crosslinked agarose
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L63/00—Compositions of epoxy resins; Compositions of derivatives of epoxy resins
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Compositions Of Macromolecular Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE7008819A SE403116B (sv) | 1970-06-25 | 1970-06-25 | Stabiliserad agarprodukt samt sett for dess stabilisering |
| SE7107180A SE414405B (sv) | 1971-06-03 | 1971-06-03 | Agarprodukt for separationsendamal, som gelmatriser for fixering av enzymer eller andra biokemiska produkter eller liknande |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE2131139A1 DE2131139A1 (de) | 1971-12-30 |
| DE2131139C2 true DE2131139C2 (de) | 1986-08-07 |
Family
ID=26654766
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE2131139A Expired DE2131139C2 (de) | 1970-06-25 | 1971-06-23 | Quervernetzte, perlförmig ausgebildete Agar- oder Agarose-Derivate, Verfahren zu ihrer Herstellung und ihre Verwendung |
Country Status (7)
| Country | Link |
|---|---|
| JP (1) | JPS5713561B1 (enrdf_load_stackoverflow) |
| CA (1) | CA995606A (enrdf_load_stackoverflow) |
| CH (1) | CH564040A5 (enrdf_load_stackoverflow) |
| DE (1) | DE2131139C2 (enrdf_load_stackoverflow) |
| FR (1) | FR2098036A5 (enrdf_load_stackoverflow) |
| GB (1) | GB1352613A (enrdf_load_stackoverflow) |
| IL (1) | IL37131A (enrdf_load_stackoverflow) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1435508A (en) * | 1972-05-01 | 1976-05-12 | Rech Et Dapplications Scient S | Process for the preparation of crosslinked polysaccharide gels |
| GB8516570D0 (en) * | 1985-07-01 | 1985-08-07 | Common Services Agency For The | Coupling reaction |
| US5998606A (en) * | 1997-11-10 | 1999-12-07 | Grandics; Peter | Mn(IV)-mediated crosslinking and functionalization of chromatography media |
| GB0316742D0 (en) | 2003-07-17 | 2003-08-20 | Fermentas Uab | Electrophoretic gels and their manufacture |
| WO2008136742A1 (en) * | 2007-05-04 | 2008-11-13 | Bio-Works Company Limited | Method for the manufacture of agarose gels |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3507851A (en) * | 1966-03-24 | 1970-04-21 | Victor Ghetie | Synthesis derivatives of agarose having application in electrophoresis on gel and in chromatography |
-
1971
- 1971-06-21 CH CH902271A patent/CH564040A5/xx not_active IP Right Cessation
- 1971-06-23 IL IL37131A patent/IL37131A/en unknown
- 1971-06-23 CA CA116,437A patent/CA995606A/en not_active Expired
- 1971-06-23 DE DE2131139A patent/DE2131139C2/de not_active Expired
- 1971-06-25 GB GB3001771A patent/GB1352613A/en not_active Expired
- 1971-06-25 FR FR7123243A patent/FR2098036A5/fr not_active Expired
- 1971-06-25 JP JP4627271A patent/JPS5713561B1/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CH564040A5 (enrdf_load_stackoverflow) | 1975-07-15 |
| CA995606A (en) | 1976-08-24 |
| DE2131139A1 (de) | 1971-12-30 |
| JPS5713561B1 (enrdf_load_stackoverflow) | 1982-03-18 |
| IL37131A0 (en) | 1971-08-25 |
| IL37131A (en) | 1976-02-29 |
| FR2098036A5 (enrdf_load_stackoverflow) | 1972-03-03 |
| GB1352613A (en) | 1974-05-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| D2 | Grant after examination | ||
| 8364 | No opposition during term of opposition |