DE19819962A1 - Hochaktive alkalische Phosphatase - Google Patents
Hochaktive alkalische PhosphataseInfo
- Publication number
- DE19819962A1 DE19819962A1 DE19819962A DE19819962A DE19819962A1 DE 19819962 A1 DE19819962 A1 DE 19819962A1 DE 19819962 A DE19819962 A DE 19819962A DE 19819962 A DE19819962 A DE 19819962A DE 19819962 A1 DE19819962 A1 DE 19819962A1
- Authority
- DE
- Germany
- Prior art keywords
- biap
- seq
- amino acid
- alkaline phosphatase
- highly active
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 102000002260 Alkaline Phosphatase Human genes 0.000 title claims description 42
- 108020004774 Alkaline Phosphatase Proteins 0.000 title claims description 42
- 230000000694 effects Effects 0.000 claims abstract description 46
- 239000002299 complementary DNA Substances 0.000 claims abstract description 32
- 150000001413 amino acids Chemical group 0.000 claims abstract description 28
- 239000012634 fragment Substances 0.000 claims abstract description 25
- 229940024606 amino acid Drugs 0.000 claims abstract description 20
- 108010044467 Isoenzymes Proteins 0.000 claims abstract description 17
- 239000013598 vector Substances 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 11
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 claims abstract description 10
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 claims abstract description 10
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 9
- 229940009098 aspartate Drugs 0.000 claims abstract description 5
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims abstract description 4
- 210000001236 prokaryotic cell Anatomy 0.000 claims abstract description 3
- 239000003513 alkali Substances 0.000 claims abstract 8
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 20
- 235000001014 amino acid Nutrition 0.000 claims description 20
- 125000000539 amino acid group Chemical group 0.000 claims description 11
- 239000004471 Glycine Substances 0.000 claims description 10
- 239000000543 intermediate Substances 0.000 claims description 9
- 238000004519 manufacturing process Methods 0.000 claims description 7
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 claims description 6
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 claims description 4
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims description 4
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 claims description 4
- 239000004474 valine Substances 0.000 claims description 4
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 claims description 3
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 claims description 3
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 claims description 3
- 239000004473 Threonine Substances 0.000 claims description 3
- 235000004279 alanine Nutrition 0.000 claims description 3
- 229910019142 PO4 Inorganic materials 0.000 claims description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 2
- 239000010452 phosphate Substances 0.000 claims description 2
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- XZKIHKMTEMTJQX-UHFFFAOYSA-L 4-nitrophenyl phosphate(2-) Chemical compound [O-][N+](=O)C1=CC=C(OP([O-])([O-])=O)C=C1 XZKIHKMTEMTJQX-UHFFFAOYSA-L 0.000 description 2
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Steroid Compounds (AREA)
Priority Applications (9)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19819962A DE19819962A1 (de) | 1998-05-05 | 1998-05-05 | Hochaktive alkalische Phosphatase |
| NZ335500A NZ335500A (en) | 1998-05-05 | 1999-04-30 | Highly active eukaryotic alkaline phosphatase and a process for producing it |
| EP99108502A EP0955369B1 (de) | 1998-05-05 | 1999-04-30 | Hochaktive alkalische Phosphatase |
| DE59913343T DE59913343D1 (de) | 1998-05-05 | 1999-04-30 | Hochaktive alkalische Phosphatase |
| AT99108502T ATE323769T1 (de) | 1998-05-05 | 1999-04-30 | Hochaktive alkalische phosphatase |
| ZA9903079A ZA993079B (en) | 1998-05-05 | 1999-05-04 | Highly active alkaline phosphatase. |
| AU26926/99A AU765392B2 (en) | 1998-05-05 | 1999-05-04 | Highly active alkaline phosphatase |
| US09/305,681 US6406899B1 (en) | 1998-05-05 | 1999-05-05 | Highly active alkaline phosphatase |
| JP12649499A JP4295386B2 (ja) | 1998-05-05 | 1999-05-06 | 高活性アルカリ性ホスファターゼ |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19819962A DE19819962A1 (de) | 1998-05-05 | 1998-05-05 | Hochaktive alkalische Phosphatase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE19819962A1 true DE19819962A1 (de) | 1999-11-11 |
Family
ID=7866683
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19819962A Withdrawn DE19819962A1 (de) | 1998-05-05 | 1998-05-05 | Hochaktive alkalische Phosphatase |
| DE59913343T Expired - Lifetime DE59913343D1 (de) | 1998-05-05 | 1999-04-30 | Hochaktive alkalische Phosphatase |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE59913343T Expired - Lifetime DE59913343D1 (de) | 1998-05-05 | 1999-04-30 | Hochaktive alkalische Phosphatase |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6406899B1 (enExample) |
| EP (1) | EP0955369B1 (enExample) |
| JP (1) | JP4295386B2 (enExample) |
| AT (1) | ATE323769T1 (enExample) |
| AU (1) | AU765392B2 (enExample) |
| DE (2) | DE19819962A1 (enExample) |
| NZ (1) | NZ335500A (enExample) |
| ZA (1) | ZA993079B (enExample) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10036491A1 (de) * | 2000-07-25 | 2002-02-07 | Roche Diagnostics Gmbh | Expression von Alkalischer Phosphatase in Hefe |
| DE10213201A1 (de) | 2002-03-25 | 2003-10-16 | Roche Diagnostics Gmbh | Erzeugung von schwach aktiven oder inaktiven Mutanten von Alkalischer Phosphatase und deren Expression in Hefe |
| EP1460425A1 (en) | 2003-03-17 | 2004-09-22 | Boehringer Mannheim Gmbh | Deglycosylated enzymes for conjugates |
| US20110142817A1 (en) * | 2004-02-04 | 2011-06-16 | Pharmaaware Sepsis B.V. | Means and method for treating and/or preventing necrotizing enterocolitis |
| WO2005074978A1 (en) * | 2004-02-04 | 2005-08-18 | Pharmaaware Sepsis B.V. | Use of alkaline phosphatase for the detoxification of lps present at mucosal barriers |
| HUE051470T2 (hu) | 2014-01-24 | 2021-03-01 | Am Pharma Bv | Egy alkalikus foszfatáz feldolgozási folyamata |
| AU2015209783B2 (en) | 2014-01-24 | 2020-10-01 | Am-Pharma B.V. | Chimeric alkaline phosphatase-like proteins |
| JP6977301B2 (ja) * | 2016-04-13 | 2021-12-08 | 東ソー株式会社 | 変異型アルカリホスファターゼをコードする遺伝子 |
| JP7692683B2 (ja) * | 2017-10-03 | 2025-06-16 | キッコーマン株式会社 | アルカリホスファターゼの製造方法及びそれを用いて得られるアルカリホスファターゼ、並びにその製造のためのベクター及び形質転換体 |
| US11338020B2 (en) | 2018-01-09 | 2022-05-24 | Synthetic Biologics, Inc. | Alkaline phosphatase agents for treatment of neurodevelopmental disorders |
| US11654184B2 (en) | 2018-03-20 | 2023-05-23 | Theriva Biologics, Inc. | Alkaline phosphatase agents for treatment of radiation disorders |
| WO2019183208A1 (en) | 2018-03-20 | 2019-09-26 | Synthetic Biologics, Inc. | Intestinal alkaline phosphatase formulations |
| US11999978B2 (en) | 2018-09-25 | 2024-06-04 | Toray Industries, Inc. | Alkaline phosphatase composition, method of producing dephosphorylated nucleic acid and method of producing labeled nucleic acid |
| EP3965805A4 (en) | 2019-05-06 | 2023-01-25 | Synthetic Biologics, Inc. | ALKALINE PHOSPHATE BASED ONCOLOGICAL TREATMENTS |
| JP7510787B2 (ja) * | 2020-05-28 | 2024-07-04 | シスメックス株式会社 | アルカリフォスファターゼ融合抗体及びその製造方法、並びに免疫測定用試薬及び免疫測定方法 |
| JP2024046503A (ja) | 2022-09-22 | 2024-04-03 | シスメックス株式会社 | 細胞外小胞に含まれるアルカリフォスファターゼの活性を測定する方法、キャリブレータ及び結合体 |
| JP2025144440A (ja) | 2024-03-19 | 2025-10-02 | シスメックス株式会社 | 試料中の細胞外小胞に含まれるアルカリホスファターゼの活性又は濃度を測定する方法、ポリペプチド分子及びその製造方法、並びにキャリブレータ |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8328918D0 (en) * | 1983-10-28 | 1983-11-30 | Unilever Plc | Alkaline phosphatase |
| ATE175998T1 (de) * | 1992-03-10 | 1999-02-15 | Jolla Cancer Res Found | Rekombinierte alkalische phosphatase aus kälberdarm |
-
1998
- 1998-05-05 DE DE19819962A patent/DE19819962A1/de not_active Withdrawn
-
1999
- 1999-04-30 NZ NZ335500A patent/NZ335500A/en unknown
- 1999-04-30 AT AT99108502T patent/ATE323769T1/de not_active IP Right Cessation
- 1999-04-30 EP EP99108502A patent/EP0955369B1/de not_active Expired - Lifetime
- 1999-04-30 DE DE59913343T patent/DE59913343D1/de not_active Expired - Lifetime
- 1999-05-04 ZA ZA9903079A patent/ZA993079B/xx unknown
- 1999-05-04 AU AU26926/99A patent/AU765392B2/en not_active Ceased
- 1999-05-05 US US09/305,681 patent/US6406899B1/en not_active Expired - Lifetime
- 1999-05-06 JP JP12649499A patent/JP4295386B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US6406899B1 (en) | 2002-06-18 |
| AU2692699A (en) | 1999-11-11 |
| ZA993079B (en) | 2000-11-06 |
| EP0955369A3 (de) | 2002-02-06 |
| JP4295386B2 (ja) | 2009-07-15 |
| EP0955369B1 (de) | 2006-04-19 |
| DE59913343D1 (de) | 2006-05-24 |
| AU765392B2 (en) | 2003-09-18 |
| ATE323769T1 (de) | 2006-05-15 |
| EP0955369A2 (de) | 1999-11-10 |
| NZ335500A (en) | 2000-02-28 |
| JPH11332586A (ja) | 1999-12-07 |
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Legal Events
| Date | Code | Title | Description |
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| 8141 | Disposal/no request for examination |