DE19702907A1 - Verfahren und Vorrichtung zur Reinigung von Nukleinsäuren - Google Patents
Verfahren und Vorrichtung zur Reinigung von NukleinsäurenInfo
- Publication number
- DE19702907A1 DE19702907A1 DE19702907A DE19702907A DE19702907A1 DE 19702907 A1 DE19702907 A1 DE 19702907A1 DE 19702907 A DE19702907 A DE 19702907A DE 19702907 A DE19702907 A DE 19702907A DE 19702907 A1 DE19702907 A1 DE 19702907A1
- Authority
- DE
- Germany
- Prior art keywords
- vessel
- sample
- nucleic acids
- nucleic acid
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 91
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 91
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 91
- 238000000034 method Methods 0.000 title claims abstract description 26
- 238000000746 purification Methods 0.000 title 1
- 238000012546 transfer Methods 0.000 claims abstract description 4
- 239000000463 material Substances 0.000 claims description 66
- 239000007788 liquid Substances 0.000 claims description 36
- 239000011521 glass Substances 0.000 claims description 12
- 238000011109 contamination Methods 0.000 claims description 9
- 230000009089 cytolysis Effects 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 4
- 238000010828 elution Methods 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 4
- 239000012528 membrane Substances 0.000 claims description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 2
- 125000003729 nucleotide group Chemical group 0.000 claims description 2
- 238000002360 preparation method Methods 0.000 claims description 2
- 239000000741 silica gel Substances 0.000 claims description 2
- 229910002027 silica gel Inorganic materials 0.000 claims description 2
- 239000007787 solid Substances 0.000 claims description 2
- GWEVSGVZZGPLCZ-UHFFFAOYSA-N Titan oxide Chemical compound O=[Ti]=O GWEVSGVZZGPLCZ-UHFFFAOYSA-N 0.000 claims 1
- 239000000835 fiber Substances 0.000 claims 1
- 229910044991 metal oxide Inorganic materials 0.000 claims 1
- 150000004706 metal oxides Chemical class 0.000 claims 1
- 229910003455 mixed metal oxide Inorganic materials 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 claims 1
- RVTZCBVAJQQJTK-UHFFFAOYSA-N oxygen(2-);zirconium(4+) Chemical compound [O-2].[O-2].[Zr+4] RVTZCBVAJQQJTK-UHFFFAOYSA-N 0.000 claims 1
- OGIDPMRJRNCKJF-UHFFFAOYSA-N titanium oxide Inorganic materials [Ti]=O OGIDPMRJRNCKJF-UHFFFAOYSA-N 0.000 claims 1
- 239000010457 zeolite Substances 0.000 claims 1
- 229910001928 zirconium oxide Inorganic materials 0.000 claims 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 239000000356 contaminant Substances 0.000 abstract 1
- 239000000523 sample Substances 0.000 description 49
- 238000002955 isolation Methods 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 239000003365 glass fiber Substances 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 230000003321 amplification Effects 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 238000001914 filtration Methods 0.000 description 5
- 238000003199 nucleic acid amplification method Methods 0.000 description 5
- 238000003752 polymerase chain reaction Methods 0.000 description 5
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 150000003839 salts Chemical class 0.000 description 4
- 238000005406 washing Methods 0.000 description 4
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000003196 chaotropic effect Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000002657 fibrous material Substances 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 238000012340 reverse transcriptase PCR Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
- 239000004365 Protease Substances 0.000 description 2
- 150000007513 acids Chemical class 0.000 description 2
- 239000000443 aerosol Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000010923 batch production Methods 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000014509 gene expression Effects 0.000 description 2
- 238000001746 injection moulding Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- -1 polyethylene Polymers 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000011534 wash buffer Substances 0.000 description 2
- 101000749837 Bos taurus Leukocyte cell-derived chemotaxin 1 Proteins 0.000 description 1
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 101710181478 Envelope glycoprotein GP350 Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 208000024556 Mendelian disease Diseases 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 108091093037 Peptide nucleic acid Proteins 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 241000726445 Viroids Species 0.000 description 1
- 238000004026 adhesive bonding Methods 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000008346 aqueous phase Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- ZRALSGWEFCBTJO-UHFFFAOYSA-O guanidinium Chemical compound NC(N)=[NH2+] ZRALSGWEFCBTJO-UHFFFAOYSA-O 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 235000021190 leftovers Nutrition 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 239000012139 lysis buffer Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000004745 nonwoven fabric Substances 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 239000011236 particulate material Substances 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 210000002307 prostate Anatomy 0.000 description 1
- 235000019419 proteases Nutrition 0.000 description 1
- 230000006920 protein precipitation Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000011146 sterile filtration Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
- 238000003828 vacuum filtration Methods 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/405—Concentrating samples by adsorption or absorption
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/56—Labware specially adapted for transferring fluids
- B01L3/563—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/56—Labware specially adapted for transferring fluids
- B01L3/563—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors
- B01L3/5635—Joints or fittings ; Separable fluid transfer means to transfer fluids between at least two containers, e.g. connectors connecting two containers face to face, e.g. comprising a filter
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0681—Filter
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
- B01L2400/0406—Moving fluids with specific forces or mechanical means specific forces capillary forces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
- B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19702907A DE19702907A1 (de) | 1997-01-28 | 1997-01-28 | Verfahren und Vorrichtung zur Reinigung von Nukleinsäuren |
| AU62132/98A AU6213298A (en) | 1997-01-28 | 1998-01-26 | Method and device for refining nucleic acids |
| EP98904143A EP0964934A1 (de) | 1997-01-28 | 1998-01-26 | Verfahren und vorrichtung zur reinigung von nukleinsäuren |
| PCT/EP1998/000408 WO1998032877A1 (de) | 1997-01-28 | 1998-01-26 | Verfahren und vorrichtung zur reinigung von nukleinsäuren |
| JP53160798A JP2001511644A (ja) | 1997-01-28 | 1998-01-26 | 核酸を精製するための方法および装置 |
| US09/355,365 US6720417B1 (en) | 1997-01-28 | 1998-01-26 | Method and device for refining nucleic acids |
| US10/786,835 US20040166524A1 (en) | 1997-01-28 | 2004-02-25 | Method and device for purifying nucleic acids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19702907A DE19702907A1 (de) | 1997-01-28 | 1997-01-28 | Verfahren und Vorrichtung zur Reinigung von Nukleinsäuren |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE19702907A1 true DE19702907A1 (de) | 1998-07-30 |
Family
ID=7818496
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE19702907A Withdrawn DE19702907A1 (de) | 1997-01-28 | 1997-01-28 | Verfahren und Vorrichtung zur Reinigung von Nukleinsäuren |
Country Status (6)
| Country | Link |
|---|---|
| US (2) | US6720417B1 (enExample) |
| EP (1) | EP0964934A1 (enExample) |
| JP (1) | JP2001511644A (enExample) |
| AU (1) | AU6213298A (enExample) |
| DE (1) | DE19702907A1 (enExample) |
| WO (1) | WO1998032877A1 (enExample) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000050870A1 (en) * | 1999-02-26 | 2000-08-31 | Mosaic Technologies | Biochemical purification devices with immobilized capture probes and their uses |
| DE10006662A1 (de) * | 2000-02-15 | 2001-08-23 | Antigen Produktions Gmbh | Gefäß zur Nukleinsäureanalytik |
| US6720417B1 (en) * | 1997-01-28 | 2004-04-13 | Roche Diagnostics Gmbh | Method and device for refining nucleic acids |
| DE102004058828A1 (de) * | 2004-10-28 | 2006-06-14 | Directif Gmbh | Vorrichtung und Verfahren zur parallelen Aufbereitung von Biopolymeren |
| EP2071037A1 (de) * | 1998-08-12 | 2009-06-17 | PreAnalytiX GmbH | Gefäß zur Entnahme von Blut |
Families Citing this family (30)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19746874A1 (de) * | 1997-10-23 | 1999-04-29 | Qiagen Gmbh | Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen |
| SE9904539D0 (sv) * | 1999-12-10 | 1999-12-10 | Alphahelix Ab | Method and device for the handling of samples and reagents |
| EP1421361B1 (de) * | 2001-08-29 | 2010-08-25 | Dahm, Michael W., Dr. Dr. | Verfahren und vorrichtung zur vorbereitung einer biologischen urprobe für die bestimmung zumindest einer darin enthaltenen komponente |
| ES2353689T5 (es) | 2003-03-14 | 2014-08-18 | Depuy Spine, Inc. | Dispositivo hidráulico de inyección de cemento óseo en vertebroplastia percutánea |
| US8066713B2 (en) | 2003-03-31 | 2011-11-29 | Depuy Spine, Inc. | Remotely-activated vertebroplasty injection device |
| US7267950B2 (en) * | 2003-05-01 | 2007-09-11 | Veridex, Lcc | Rapid extraction of RNA from cells and tissues |
| US8415407B2 (en) | 2004-03-21 | 2013-04-09 | Depuy Spine, Inc. | Methods, materials, and apparatus for treating bone and other tissue |
| WO2005030034A2 (en) | 2003-09-26 | 2005-04-07 | Depuy Spine, Inc. | Device for delivering viscous material |
| US7897378B2 (en) | 2004-03-18 | 2011-03-01 | Roche Molecular Systems, Inc. | Method and device for purifying nucleic acids |
| EP1786343B1 (en) | 2004-07-30 | 2012-05-02 | Depuy Spine, Inc. | Apparatus for treating bone and other tissue |
| US20060166223A1 (en) * | 2005-01-26 | 2006-07-27 | Reed Michael W | DNA purification and analysis on nanoengineered surfaces |
| US9381024B2 (en) | 2005-07-31 | 2016-07-05 | DePuy Synthes Products, Inc. | Marked tools |
| US9918767B2 (en) | 2005-08-01 | 2018-03-20 | DePuy Synthes Products, Inc. | Temperature control system |
| US8360629B2 (en) | 2005-11-22 | 2013-01-29 | Depuy Spine, Inc. | Mixing apparatus having central and planetary mixing elements |
| US7608399B2 (en) * | 2006-06-26 | 2009-10-27 | Blood Cell Storage, Inc. | Device and method for extraction and analysis of nucleic acids from biological samples |
| KR100805449B1 (ko) | 2006-08-18 | 2008-02-20 | 배정훈 | 비석분말을 분자체로 이용하여 아가로스겔에서 dna를정제하는 방법 |
| AU2007297097A1 (en) | 2006-09-14 | 2008-03-20 | Depuy Spine, Inc. | Bone cement and methods of use thereof |
| WO2008047371A2 (en) * | 2006-10-19 | 2008-04-24 | Depuy Spine, Inc. | Fluid delivery system |
| WO2008075501A1 (ja) | 2006-12-19 | 2008-06-26 | Konica Minolta Medical & Graphic, Inc. | 回転式抽出容器、それを用いた細胞種の同定方法、遺伝子検出方法、及び自動核酸抽出装置 |
| JP2010537797A (ja) * | 2007-05-23 | 2010-12-09 | ニプロ インク | 真空濾過システム中の泡の制御のための方法及び装置 |
| WO2009014768A1 (en) * | 2007-07-26 | 2009-01-29 | Nypro Inc. | Method and apparatus for filtrate storage handling |
| EP2183040B1 (en) | 2007-07-26 | 2012-03-07 | Roush Life Sciences, LLC | Methods and apparatus for supporting a vacuum filtration device |
| US20090048438A1 (en) * | 2007-08-13 | 2009-02-19 | Taigen Bioscience Corporation. | Method for washing a column and method for extracting membrane-bound target molecules |
| EP2055385B1 (en) | 2007-10-31 | 2010-05-26 | Roche Diagnostics GmbH | Method and device for purifying nucleic acids |
| WO2009117167A1 (en) * | 2008-01-02 | 2009-09-24 | Blood Cell Storage, Inc. | Devices and processes for nucleic acid extraction |
| CN102264899A (zh) * | 2008-11-04 | 2011-11-30 | 血细胞保存公司 | 弯曲的玻璃表面上的核酸提取 |
| WO2012159063A2 (en) | 2011-05-19 | 2012-11-22 | Blood Cell Strorage, Inc. | Gravity flow fluidic device for nucleic acid extraction |
| EP3003561B1 (en) * | 2013-05-24 | 2018-12-19 | Occam Biolabs, Inc. | System and method for collecting a sample of nucleic acid |
| AU2015349719A1 (en) | 2014-11-21 | 2017-07-13 | Occam Biolabs, Inc. | System and method for collecting a sample of nucleic acid |
| US11440000B2 (en) * | 2017-07-27 | 2022-09-13 | Biomerieux, Inc. | Isolation tube with an endcap |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU6870091A (en) | 1989-11-08 | 1991-06-13 | Fmc Corporation | Combined centrifuge tube and porous selection means for separation and recovery of biological materials |
| US5438128A (en) | 1992-02-07 | 1995-08-01 | Millipore Corporation | Method for rapid purifiction of nucleic acids using layered ion-exchange membranes |
| DE69433425T2 (de) * | 1993-08-30 | 2004-10-07 | Promega Corp | Zusammensetzungen und verfahren zur reinigung von nukleinsäuren |
| WO1995027546A1 (en) | 1994-04-08 | 1995-10-19 | Amicon, Inc. | Method and apparatus for isolation and purification of biologically active complexes |
| CA2198757A1 (en) | 1994-08-29 | 1996-03-07 | Govert Arnoldus Petrus Borst | Device for use in the isolation of a biological material such as nucleic acid |
| DE19512369A1 (de) * | 1995-04-01 | 1996-10-02 | Boehringer Mannheim Gmbh | Vorrichtung zur Isolierung von Nukleinsäuren |
| EP0734768B1 (de) * | 1995-04-01 | 2002-08-28 | Roche Diagnostics GmbH | Verfahren zur Bindung eines biologischen Materials |
| US5733449A (en) | 1995-12-08 | 1998-03-31 | Orbital Biosciences, Llc | Microconcentrator device |
| DE19702907A1 (de) * | 1997-01-28 | 1998-07-30 | Boehringer Mannheim Gmbh | Verfahren und Vorrichtung zur Reinigung von Nukleinsäuren |
| DE19725190A1 (de) * | 1997-06-14 | 1998-12-17 | Innova Gmbh | Vorrichtungen mit integrierten Elektroden aus elektrisch leitfähigen Kunststoffen |
| JP2001516731A (ja) * | 1997-09-17 | 2001-10-02 | ジエントラ・システムズ・インコーポレーテツド | 核酸を単離するための装置および方法 |
-
1997
- 1997-01-28 DE DE19702907A patent/DE19702907A1/de not_active Withdrawn
-
1998
- 1998-01-26 EP EP98904143A patent/EP0964934A1/de not_active Withdrawn
- 1998-01-26 JP JP53160798A patent/JP2001511644A/ja not_active Withdrawn
- 1998-01-26 AU AU62132/98A patent/AU6213298A/en not_active Abandoned
- 1998-01-26 WO PCT/EP1998/000408 patent/WO1998032877A1/de not_active Ceased
- 1998-01-26 US US09/355,365 patent/US6720417B1/en not_active Expired - Fee Related
-
2004
- 2004-02-25 US US10/786,835 patent/US20040166524A1/en not_active Abandoned
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6720417B1 (en) * | 1997-01-28 | 2004-04-13 | Roche Diagnostics Gmbh | Method and device for refining nucleic acids |
| EP2071037A1 (de) * | 1998-08-12 | 2009-06-17 | PreAnalytiX GmbH | Gefäß zur Entnahme von Blut |
| EP2206792A1 (de) * | 1998-08-12 | 2010-07-14 | PreAnalytiX GmbH | Gefäß zur Entnahme von Blut |
| WO2000050870A1 (en) * | 1999-02-26 | 2000-08-31 | Mosaic Technologies | Biochemical purification devices with immobilized capture probes and their uses |
| DE10006662A1 (de) * | 2000-02-15 | 2001-08-23 | Antigen Produktions Gmbh | Gefäß zur Nukleinsäureanalytik |
| WO2001060517A2 (de) | 2000-02-15 | 2001-08-23 | Antigene Biotech Gmbh | Gefäss zur nukleinsäureanalytik |
| WO2001060517A3 (de) * | 2000-02-15 | 2002-08-22 | Antigen Produktions Gmbh | Gefäss zur nukleinsäureanalytik |
| AU2001235479B2 (en) * | 2000-02-15 | 2004-10-14 | Preanalytix Gmbh | Container for nucleic acid analysis |
| EP2189220A1 (de) * | 2000-02-15 | 2010-05-26 | PreAnalytiX GmbH | Gefäss zur Nukleinsäureanalytik |
| EP2189220B1 (de) | 2000-02-15 | 2016-06-08 | PreAnalytiX GmbH | Verfahren zur Nukleinsäureanalytik |
| DE102004058828A1 (de) * | 2004-10-28 | 2006-06-14 | Directif Gmbh | Vorrichtung und Verfahren zur parallelen Aufbereitung von Biopolymeren |
| DE102004058828B4 (de) * | 2004-10-28 | 2010-08-19 | Progen Biotechnik Gmbh | Vorrichtung und Verfahren zur parallelen Aufbereitung von Biopolymeren |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2001511644A (ja) | 2001-08-14 |
| EP0964934A1 (de) | 1999-12-22 |
| US6720417B1 (en) | 2004-04-13 |
| WO1998032877A1 (de) | 1998-07-30 |
| US20040166524A1 (en) | 2004-08-26 |
| AU6213298A (en) | 1998-08-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE19702907A1 (de) | Verfahren und Vorrichtung zur Reinigung von Nukleinsäuren | |
| EP0738733B1 (de) | Vorrichtung zur Isolierung von Nukleinsäuren | |
| DE602005001156T2 (de) | Verfahren und vorrichtung zur reinigung von nukleinsäuren | |
| EP0819255B1 (de) | System zur freisetzung und isolierung von nukleinsäuren | |
| DE4143639C2 (de) | Verfahren zur Isolierung und Reinigung von Nukleinsäuren | |
| EP1121460B1 (de) | Verfahren und mittel zur isolierung und reinigung von nukleinsäuren an oberflächen | |
| EP0687502B1 (de) | Verfahren und Vorrichtung zur Behandlung von Nukleinsäuren aus einer Probe | |
| DE69223617T2 (de) | Verfahren zum Auffangen von Zellkernen und dafür verwendete Vorrichtung | |
| EP2356232B1 (de) | Verfahren zur aufreinigung von nukleinsäuren, insbesondere aus fixiertem gewebe | |
| EP1421361B1 (de) | Verfahren und vorrichtung zur vorbereitung einer biologischen urprobe für die bestimmung zumindest einer darin enthaltenen komponente | |
| EP0555270A1 (de) | Verfahren zur isolierung von nukleinsäuren aus zellsuspensionen. | |
| DE29505652U1 (de) | Gefäß zur kontaminationsreduzierten Behandlung von Flüssigkeiten | |
| EP0734768A1 (de) | Verfahren zur Bindung eines biologischen Materials | |
| EP1274745A1 (de) | Magnetische, silanisierte trägermaterialien auf basis von polyvinylalkohol | |
| EP1123980B1 (de) | System zur einfachen Nukleinsäureanalytik | |
| DE69829466T2 (de) | Biomolekularer prozessor | |
| DE202015104264U1 (de) | Vorrichtung zur Aufreinigung von Nukleinsäuren | |
| EP2880147B1 (de) | Vorrichtung und verfahren zur behandlung eines filtrationsmediums | |
| DE19605814A1 (de) | Vorrichtung und Verfahren zur Aufarbeitung einer Mehrzahl von Proben | |
| DE202004006675U1 (de) | Vorrichtung zur Reinigung von Nukleinsäuren | |
| DE19512360A1 (de) | Verfahren zur Bindung eines biologischen Materials |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8127 | New person/name/address of the applicant |
Owner name: ROCHE DIAGNOSTICS GMBH, 68305 MANNHEIM, DE |
|
| 8141 | Disposal/no request for examination |