DE151475T1 - Adult-t-zell-leukemiavirus-antigenpolypeptid. - Google Patents

Adult-t-zell-leukemiavirus-antigenpolypeptid.

Info

Publication number
DE151475T1
DE151475T1 DE198585101064T DE85101064T DE151475T1 DE 151475 T1 DE151475 T1 DE 151475T1 DE 198585101064 T DE198585101064 T DE 198585101064T DE 85101064 T DE85101064 T DE 85101064T DE 151475 T1 DE151475 T1 DE 151475T1
Authority
DE
Germany
Prior art keywords
adult
recombinant plasmid
enzyme
cell leukemia
microorganism
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE198585101064T
Other languages
English (en)
Inventor
Haruo Suginami-Ku Tokyo Sugano
Mitsuaki Meguro-Ku Tokyo Yoshida
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japanese Foundation for Cancer Research
Original Assignee
Japanese Foundation for Cancer Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP59016951A external-priority patent/JPS60166699A/ja
Priority claimed from JP59046686A external-priority patent/JPH07103160B2/ja
Application filed by Japanese Foundation for Cancer Research filed Critical Japanese Foundation for Cancer Research
Publication of DE151475T1 publication Critical patent/DE151475T1/de
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/5748—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving oncogenic proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/13011—Gammaretrovirus, e.g. murine leukeamia virus
    • C12N2740/13022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/806—Antigenic peptides or proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/82—Proteins from microorganisms
    • Y10S530/825—Bacteria

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Claims (17)

EP 85 10 1064.5 Juridical Foundation, Japanese Foundation for Cancer Research P at entansprüche 5 (Deutsche Übersetzung nach Artikel 67 EPÜ)
1. Antigenes Polypeptid, dadurch gekennzeichnet, daß es vom env-Gen des Erwachsenen—T-Zell-Leukämie-Virus oder einem Teil davon codiert wird.
2. Fusionsprotein, dadurch gekennzeichnet, daß es aus einem vom env-Gen des Erwachsenen-T-Zell-Leukämie-Virus oder einem Teil davon codierten antigenen Polypeptid und einem Enzym besteht.
3. Fusionsprotein nach Anspruch 2, dadurch gekennzeichnet, daß das Enzym die ß-Galactosidase ist.
4. Rekombinantes Plasmid, dadurch gekennzeichnet, daß es
ein DNA-Fragment enthaltend das env-Gen des Erwachsenen— T-Zell-Leukämie-Virus oder einen Teil davon enthält.
5. Rekombihantes Plasmid nach Anspruch 4, dadurch gekennzeichnet, daß es ein zusätzliches für ein Enzym codierendes Gen enthält.
6. Rekombinantes Plasmid nach Anspruch 5, dadurch gekennzeichnet, daß das Enzym die ß-Galactosidase ist.
7. Rekombinantes Plasmid nach Anspruch 6, dadurch gekennzeichnet, daß es pEH9 oder pEA1 ist.
8. Verfahren zur Herstellung eines antigenen Polypeptids, das vom env-Geh des Erwachsenen—T-Zell-Leukämie-Virus oder von einem Teil davon codiert wird, dadurch gekennzeichnet, daß man in einem Medium einen Mikroorganismus
züchtet, der ein rekombinantes Plasmid trägt, in welches ein DNA-Fragment, enthaltend das env—Gen des Erwachsenen-T-Zell-Leukämie-Virus oder einen Teil davon, eingesetzt ist, das vom env—Gen oder einem Teil davon codierte antigene Polypeptid des Erwachsenen—T-ZeIl-Leukämie-Virus im Kulturmedium ansammelt und das Polypeptid daraus wiedergewinnt.
9. Verfahren nach Anspruch 8, dadurch gekennzeichnet, daß das rekombinante Plasmid zusätzlich ein für ein Enzym codierendes Gen trägt.
10. Verfahren nach Anspruch 9, dadurch gekennzeichnet, daß das Enzym die ß-Galactosidase ist.
11. Verfahren nach Anspruch 8, 9 oder 10, dadurch gekennzeichnet, daß der Mikroorganismus zur Gattung Escherichia gehört.
12. Verfahren nach Anspruch 11, dadurch gekennzeichnet, daß der Mikroorganismus zu Escherichia coli gehört.
13. Verfahren nach Anspruch 8, dadurch gekennzeichnet, daß das rekombinante Plasmid pEH9 öder pEA1 ist.
14. Mikroorganismus, dadurch gekennzeichnet, daß er ein rekombinantes Plasmid enthält, in welches ein das env-Gen des Erwachsenen-T-Zell-Leukämie-Virus oder einen Teil davon enthaltendes DNA-Fragment eingesetzt ist.
15. Mikroorganismus nach Anspruch 14, dadurch gekennzeichnet, daß das rekombinante Plasmid zusätzlich ein für ein Enzym codierendes Gen trägt.
16. Mikroorganismus nach Anspruch 15, dadurch gekennzeichnet, daß das Enzym die ß-Galactosidase ist.
1
17. Mikroorganxsmus nach Anspruch 14, dadurch gekennzeichnet, daß er zu Escherichia coli gehört.
DE198585101064T 1984-02-03 1985-02-01 Adult-t-zell-leukemiavirus-antigenpolypeptid. Pending DE151475T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP59016951A JPS60166699A (ja) 1984-02-03 1984-02-03 成人ｔ細胞白血病ウイルス抗原ポリペプチド
JP59046686A JPH07103160B2 (ja) 1984-03-12 1984-03-12 成人ｔ細胞白血病ウイルス抗原ポリペプチド

Publications (1)

Publication Number Publication Date
DE151475T1 true DE151475T1 (de) 1986-02-13

Family

ID=26353401

Family Applications (2)

Application Number Title Priority Date Filing Date
DE198585101064T Pending DE151475T1 (de) 1984-02-03 1985-02-01 Adult-t-zell-leukemiavirus-antigenpolypeptid.
DE8585101064T Expired - Lifetime DE3579009D1 (de) 1984-02-03 1985-02-01 Adult-t-zell-leukemiavirus-antigenpolypeptid.

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE8585101064T Expired - Lifetime DE3579009D1 (de) 1984-02-03 1985-02-01 Adult-t-zell-leukemiavirus-antigenpolypeptid.

Country Status (4)

Country Link
US (1) US4724258A (de)
EP (1) EP0151475B1 (de)
CA (1) CA1339361C (de)
DE (2) DE151475T1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4525300A (en) * 1983-01-07 1985-06-25 Japanese Foundation For Cancer Research Human leukemia virus-related peptides, antibodies of the peptides and a process for production of the antibodies
EP0152030A3 (de) * 1984-02-03 1986-08-13 Juridical Foundation Japanese Foundation for Cancer Research Adult-T-Zell-Leukemiavirus-Antigenpolypeptid
JPS60169498A (ja) * 1984-02-10 1985-09-02 Kyowa Hakko Kogyo Co Ltd 成人ｔ細胞白血病ウイルス抗原ペプチド誘導体
EP0203177A4 (de) * 1984-11-29 1987-04-28 Scripps Clinic Res Polypeptide und antikörper gegen entglykosilierte virale glykoproteine.
JP2564268B2 (ja) * 1985-08-28 1996-12-18 協和醗酵工業株式会社 融合抗原ポリペプチド
US5643714A (en) * 1986-12-31 1997-07-01 Genelabs Technologies, Inc. Method and assay for HTLV
US5614366A (en) * 1986-12-31 1997-03-25 Genelabs Technologies, Inc. HTLV-I peptide antigens and kit
US20030022826A1 (en) * 1987-09-08 2003-01-30 Duke University Use of synthetic peptides to induce tolerance to pathogenic T and B cell epitopes of autoantigens or infectious agents
US5516632A (en) * 1988-02-08 1996-05-14 Duke University Synthetic peptides
AU5827590A (en) * 1989-06-01 1991-01-07 E.I. Du Pont De Nemours And Company Htlv-1 env gene products expressed in (e. coli)
US5882853A (en) * 1989-09-01 1999-03-16 The United States Of America As Represented By The Department Of Health And Human Services Method of evaluating HTLV-I-specific T cell responses
ES2072346T3 (es) * 1989-10-23 1995-07-16 Hoffmann La Roche Peptidos sinteticos de la cubierta del htlv-i.
AU7258491A (en) * 1990-02-09 1991-09-03 E.I. Du Pont De Nemours And Company Htlv envelope antigenic segment
EP0618804A4 (de) * 1991-10-08 1995-06-07 Univ Duke Antigenen Determinanten von HTLV entsprechende Peptide.

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4572800A (en) * 1982-09-30 1986-02-25 Otsuka Pharmaceutical Co., Ltd. Human leukemia virus-related peptides, a process for production thereof, antibodies of the peptides and a process for production of the antibodies
JPS59104325A (ja) * 1982-12-07 1984-06-16 Japan Found Cancer ヒト白血病ウイルスの遺伝子ｒｎａに相補性を示すｄｎａ
AU583473B2 (en) * 1983-04-27 1989-05-04 President And Fellows Of Harvard College Method and products for detection of human t cell leukemia virus
EP0152030A3 (de) * 1984-02-03 1986-08-13 Juridical Foundation Japanese Foundation for Cancer Research Adult-T-Zell-Leukemiavirus-Antigenpolypeptid
US4520113A (en) * 1984-04-23 1985-05-28 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Serological detection of antibodies to HTLV-III in sera of patients with AIDS and pre-AIDS conditions

Also Published As

Publication number Publication date
EP0151475A2 (de) 1985-08-14
CA1339361C (en) 1997-08-26
EP0151475B1 (de) 1990-08-08
US4724258A (en) 1988-02-09
EP0151475A3 (en) 1986-08-13
DE3579009D1 (de) 1990-09-13

Similar Documents

Publication Publication Date Title
DE151475T1 (de) Adult-t-zell-leukemiavirus-antigenpolypeptid.
DE214555T1 (de) Fusionspolypeptid mit antigener wirkung.
SG49097A1 (en) Nucleotide sequences coding for a protein with urease activity
EP0174367A4 (de) Menge und verfahren zur herstellung rekombinierter erzeugnisse in grosser ausbeute.
ATE121781T1 (de) Verwendung von dns-fragmenten, die ein signalpeptid kodieren zur sekretion von reifen proteinen in rekombinanten hefen, expressions- kassetten, transformierte hefen und verfahren zur herstellung dieser proteine.
ES8308534A1 (es) Un metodo de producir un polipeptido que exhibe actividad inmunologica o biologica del interferon de leucocitos humanos.
IT8119760A0 (it) Polipeptide prodotto microbiologicamente con la sequenza di aminoacidi dell'interferone umano, dna e plasmidi che codificano per questa sequenza, microorganismi che contengono queste informazioni genetiche e processo per la loro preparazione.
IL77369A (en) Alpha, beta or omega horse interferon, their preparation and pharmaceutical compositions containing them, recombinant dna coding therefor and transformed host organisms containing such dna
WO1988003173A3 (en) New forms of colony stimulating factor-1
ATE284954T1 (de) Hyaluronan synthase gen und seine verwendung
HUT46061A (en) Dna sequences encoding hirudin-like protein and process for producing such proteins
HUT63652A (en) Process for producing cd44 surface protein variants, dna sequences encoding them and antibodies against them, as well as their application in diagnosis and therapy
DK1396542T3 (da) P-selectin ligand protein
ES555124A0 (es) Procedimiento de preparar particulas que contienen secuencias de aminoacidos y que tienen propiedades inmunogenas
DE152030T1 (de) Adult-t-zell-leukemiavirus-antigenpolypeptid.
EP0139878A3 (de) Herstellung von Immun-Interferon und seiner m-RNS
IL75963A (en) Omega interferon polypeptides of type 1,n-glycosylated derivatives thereof with omega interferon activity,polynucleotide sequences encoding the omega interferon polypeptides,methods for the preparation of the polypeptides and pharmaceutical compositions containing them
DE146944T1 (de) Menschliches gamma-interferonpolypeptidderivat.
DE3667488D1 (de) Verfahren zur herstellung von glucagon oder dessen derivaten in einem transformierten hefestamm.
DE152076T1 (de) Derivat des adult-t-zell-leukemiavirus-antigenpeptids.
ES8609360A1 (es) Procedimiento para preparar peptidos
EP0835661A3 (de) Menslisches Interferon-Beta 2A zur Verwendung als Arzneimittel
HU895573D0 (en) Process for producing human fibroblast growth factor derivatives
EP0054330A3 (en) Dna sequences encoding the various allelic forms of mature thaumatin, and cloning vehicles comprising said dna's and their use in transforming microorganisms
DE3583634D1 (de) Gentechnologisches verfahren zur herstellung von human-interleukin-2 und mittel zur durchfuehrung dieses verfahrens.