DE112011103187B4 - System und Verfahren zur 3D-Lokalisierungsmikroskopie - Google Patents

System und Verfahren zur 3D-Lokalisierungsmikroskopie Download PDF

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DE112011103187B4
DE112011103187B4 DE112011103187.4T DE112011103187T DE112011103187B4 DE 112011103187 B4 DE112011103187 B4 DE 112011103187B4 DE 112011103187 T DE112011103187 T DE 112011103187T DE 112011103187 B4 DE112011103187 B4 DE 112011103187B4
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image
phase
emitter
localization microscopy
microscopy system
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DE112011103187T5 (de
DE112011103187T8 (de
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Christian Soeller
David Michael Baddeley
Mark Bryden Cannell
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Carl Zeiss Microscopy GmbH
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/0092—Polarisation microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/14—Condensers affording illumination for phase-contrast observation
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/58—Optics for apodization or superresolution; Optical synthetic aperture systems
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06F—ELECTRIC DIGITAL DATA PROCESSING
    • G06F18/00—Pattern recognition
    • G06F18/20—Analysing
    • G06F18/24—Classification techniques
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/194—Segmentation; Edge detection involving foreground-background segmentation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/70—Determining position or orientation of objects or cameras
    • G06T7/73—Determining position or orientation of objects or cameras using feature-based methods
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N13/00—Stereoscopic video systems; Multi-view video systems; Details thereof
    • H04N13/20—Image signal generators
    • H04N13/204—Image signal generators using stereoscopic image cameras
    • H—ELECTRICITY
    • H04—ELECTRIC COMMUNICATION TECHNIQUE
    • H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
    • H04N17/00—Diagnosis, testing or measuring for television systems or their details
    • H04N17/002—Diagnosis, testing or measuring for television systems or their details for television cameras
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image

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  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Multimedia (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Theoretical Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Signal Processing (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Data Mining & Analysis (AREA)
  • Biomedical Technology (AREA)
  • Quality & Reliability (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Artificial Intelligence (AREA)
  • Evolutionary Biology (AREA)
  • Evolutionary Computation (AREA)
  • General Engineering & Computer Science (AREA)
  • Microscoopes, Condenser (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Image Processing (AREA)
  • Image Analysis (AREA)
DE112011103187.4T 2010-09-24 2011-09-26 System und Verfahren zur 3D-Lokalisierungsmikroskopie Active DE112011103187B4 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
NZ588199 2010-09-24
NZ58819910 2010-09-24
PCT/NZ2011/000200 WO2012039636A2 (en) 2010-09-24 2011-09-26 "3d localisation microscopy and 4d localisation microscopy and tracking methods and systems"

Publications (3)

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DE112011103187T5 DE112011103187T5 (de) 2013-07-25
DE112011103187T8 DE112011103187T8 (de) 2013-09-26
DE112011103187B4 true DE112011103187B4 (de) 2021-10-14

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US (3) US9523846B2 (https=)
JP (2) JP2013539074A (https=)
DE (1) DE112011103187B4 (https=)
WO (1) WO2012039636A2 (https=)

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DE102013208415B4 (de) 2013-05-07 2023-12-28 Carl Zeiss Microscopy Gmbh Mikroskop und Verfahren für die 3D-hochauflösende Lokalisierungsmikroskopie

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DE102012201003B4 (de) * 2012-01-24 2024-07-25 Carl Zeiss Microscopy Gmbh Mikroskop und Verfahren für die hochauflösende 3-D Fluoreszenzmikroskopie
DE102012201286B4 (de) 2012-01-30 2025-07-24 Carl Zeiss Microscopy Gmbh Mikroskop und Verfahren für die wellenlängenselektive und örtlich hochauflösende Mikroskopie
DE102012202730B4 (de) * 2012-02-22 2025-07-24 Carl Zeiss Microscopy Gmbh Wellenlängenselektive und örtlich hochauflösende Fluoreszenzmikroskopie
EP2660639B1 (en) * 2012-05-02 2016-04-13 Centre National De La Recherche Scientifique Method and apparatus for single-particle localization using wavelet analysis
JP6106956B2 (ja) * 2012-05-31 2017-04-05 株式会社ニコン 顕微鏡装置
DE102013208926A1 (de) 2013-05-14 2014-11-20 Carl Zeiss Microscopy Gmbh Verfahren zur 3D-hochauflösenden Lokalisierungsmikroskopie
DE102013208927A1 (de) 2013-05-14 2014-11-20 Carl Zeiss Microscopy Gmbh Verfahren zur 3D-hochauflösenden Lokalisierungsmikroskopie
DE102013015933A1 (de) * 2013-09-19 2015-03-19 Carl Zeiss Microscopy Gmbh Hochauflösende Scanning-Mikroskopie
DE102013016368B4 (de) * 2013-09-30 2024-05-16 Carl Zeiss Microscopy Gmbh Lichtmikroskop und Mikroskopieverfahren zum Untersuchen einer mikroskopischen Probe
EP3382439B1 (en) * 2015-11-27 2025-12-10 Nikon Corporation Microscope, observation method, and image processing program
CN105403170B (zh) * 2015-12-11 2018-05-25 华侨大学 一种显微3d形貌测量方法及装置
US10908072B2 (en) 2016-12-15 2021-02-02 The Board Of Regents Of The University Of Texas System Total internal reflection and transmission illumination fluorescence microscopy imaging system with improved background suppression
EP3561766A4 (en) * 2016-12-21 2020-07-01 Nikon Corporation INFORMATION PROCESSING DEVICE, IMAGE PROCESSING DEVICE, MICROSCOPE, INFORMATION PROCESSING METHOD AND PROGRAM
DE102017105103A1 (de) 2017-03-10 2018-09-13 Carl Zeiss Microscopy Gmbh 3D-Mikroskopie
JP7144438B2 (ja) * 2017-04-04 2022-09-29 ザ ユニバーシティ オブ ユタ リサーチ ファウンデイション 顕微鏡における高精度波長抽出用の位相板
CN107133964B (zh) * 2017-06-01 2020-04-24 江苏火米互动科技有限公司 一种基于Kinect的抠像方法
US11169368B2 (en) * 2018-01-02 2021-11-09 King's College London Method and system for localisation microscopy
CN109080144A (zh) * 2018-07-10 2018-12-25 泉州装备制造研究所 基于中心点判断的3d打印喷头末端实时跟踪定位方法
CN108982454B (zh) * 2018-07-30 2021-03-02 华中科技大学苏州脑空间信息研究院 一种轴向多层并行扫描显微成像方法及系统
US20200056615A1 (en) 2018-08-16 2020-02-20 Saudi Arabian Oil Company Motorized pump
US20200056462A1 (en) 2018-08-16 2020-02-20 Saudi Arabian Oil Company Motorized pump
DE102018122652B4 (de) * 2018-09-17 2025-11-27 Carl Zeiss Microscopy Gmbh Spektralauflösende, hochauflösende 3D-Lokalisierungmikroskopie
DE102018128590A1 (de) 2018-11-14 2020-05-14 Carl Zeiss Microscopy Gmbh Fluktuationsbasierte Fluoreszenzmikroskopie
US11347040B2 (en) * 2019-02-14 2022-05-31 Double Helix Optics Inc. 3D target for optical system characterization
DE102020113998A1 (de) 2020-05-26 2021-12-02 Abberior Instruments Gmbh Verfahren, Computerprogramm und Vorrichtung zum Bestimmen von Positionen von Molekülen in einer Probe
US11371326B2 (en) 2020-06-01 2022-06-28 Saudi Arabian Oil Company Downhole pump with switched reluctance motor
US11499563B2 (en) 2020-08-24 2022-11-15 Saudi Arabian Oil Company Self-balancing thrust disk
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US11994016B2 (en) 2021-12-09 2024-05-28 Saudi Arabian Oil Company Downhole phase separation in deviated wells
US12258954B2 (en) 2021-12-15 2025-03-25 Saudi Arabian Oil Company Continuous magnetic positive displacement pump
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DE102022200841B3 (de) 2022-01-26 2023-05-04 Carl Zeiss Microscopy Gmbh Verfahren, Anordnung und Mikroskop zur dreidimensionalen Bildgebung in der Mikroskopie unter Nutzung einer asymmetrischen Punktbildübertragungsfunktion
JP7673010B2 (ja) * 2022-03-22 2025-05-08 株式会社日立ハイテク 光電子顕微鏡

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Cited By (1)

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Publication number Priority date Publication date Assignee Title
DE102013208415B4 (de) 2013-05-07 2023-12-28 Carl Zeiss Microscopy Gmbh Mikroskop und Verfahren für die 3D-hochauflösende Lokalisierungsmikroskopie

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JP6416160B2 (ja) 2018-10-31
US20130300833A1 (en) 2013-11-14
US10571674B2 (en) 2020-02-25
WO2012039636A3 (en) 2012-07-12
US10007103B2 (en) 2018-06-26
JP2016197260A (ja) 2016-11-24
WO2012039636A2 (en) 2012-03-29
US20180348499A1 (en) 2018-12-06
DE112011103187T5 (de) 2013-07-25
JP2013539074A (ja) 2013-10-17
US20170168283A1 (en) 2017-06-15
DE112011103187T8 (de) 2013-09-26
US9523846B2 (en) 2016-12-20

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