DE102012201003B4 - Mikroskop und Verfahren für die hochauflösende 3-D Fluoreszenzmikroskopie - Google Patents
Mikroskop und Verfahren für die hochauflösende 3-D Fluoreszenzmikroskopie Download PDFInfo
- Publication number
- DE102012201003B4 DE102012201003B4 DE102012201003.3A DE102012201003A DE102012201003B4 DE 102012201003 B4 DE102012201003 B4 DE 102012201003B4 DE 102012201003 A DE102012201003 A DE 102012201003A DE 102012201003 B4 DE102012201003 B4 DE 102012201003B4
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
- G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
- G02B27/58—Optics for apodization or superresolution; Optical synthetic aperture systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/061—Sources
- G01N2201/06113—Coherent sources; lasers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/12—Circuits of general importance; Signal processing
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- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Optics & Photonics (AREA)
- Engineering & Computer Science (AREA)
- Multimedia (AREA)
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Microscoopes, Condenser (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102012201003.3A DE102012201003B4 (de) | 2012-01-24 | 2012-01-24 | Mikroskop und Verfahren für die hochauflösende 3-D Fluoreszenzmikroskopie |
| US14/373,740 US9885860B2 (en) | 2012-01-24 | 2012-12-13 | Microscope and method for high-resolution 3D fluorescence microscopy |
| PCT/EP2012/075465 WO2013110408A1 (de) | 2012-01-24 | 2012-12-13 | Mikroskop und verfahren für die hochauflösende 3-d fluoreszenzmikroskopie |
| JP2014552544A JP6148256B2 (ja) | 2012-01-24 | 2012-12-13 | 高分解能3d蛍光顕微鏡法のための顕微鏡および方法 |
| EP12809201.2A EP2807515B1 (de) | 2012-01-24 | 2012-12-13 | Mikroskop und verfahren für die hochauflösende 3-d fluoreszenzmikroskopie |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102012201003.3A DE102012201003B4 (de) | 2012-01-24 | 2012-01-24 | Mikroskop und Verfahren für die hochauflösende 3-D Fluoreszenzmikroskopie |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE102012201003A1 DE102012201003A1 (de) | 2013-07-25 |
| DE102012201003B4 true DE102012201003B4 (de) | 2024-07-25 |
Family
ID=47471763
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE102012201003.3A Active DE102012201003B4 (de) | 2012-01-24 | 2012-01-24 | Mikroskop und Verfahren für die hochauflösende 3-D Fluoreszenzmikroskopie |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US9885860B2 (enExample) |
| EP (1) | EP2807515B1 (enExample) |
| JP (1) | JP6148256B2 (enExample) |
| DE (1) | DE102012201003B4 (enExample) |
| WO (1) | WO2013110408A1 (enExample) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102012201003B4 (de) | 2012-01-24 | 2024-07-25 | Carl Zeiss Microscopy Gmbh | Mikroskop und Verfahren für die hochauflösende 3-D Fluoreszenzmikroskopie |
| DE102013106895B4 (de) * | 2013-07-01 | 2015-09-17 | Leica Microsystems Cms Gmbh | Lichtmikroskopisches Verfahren zur Lokalisierung von Punktobjekten |
| JP2016206652A (ja) * | 2015-04-21 | 2016-12-08 | オリンパス株式会社 | 試料の3次元構造の撮像方法及び顕微鏡装置 |
| WO2017090210A1 (ja) * | 2015-11-27 | 2017-06-01 | 株式会社ニコン | 顕微鏡、観察方法、及び画像処理プログラム |
| CN109154569A (zh) | 2016-02-12 | 2019-01-04 | 麻省理工学院 | 用于对未切片组织样本成像的方法和装置 |
| DE102017129519B4 (de) * | 2017-12-12 | 2020-08-06 | Technische Universität Ilmenau | Anordnung und Verfahren zur simultanen Messung der Fluoreszenz einzelner Schichten in einem Schichtsystem, beispielsweise dem Augenhintergrund |
| GB2578769B (en) | 2018-11-07 | 2022-07-20 | Advanced Risc Mach Ltd | Data processing systems |
| DE102018220779A1 (de) * | 2018-12-03 | 2020-06-04 | Carl Zeiss Microscopy Gmbh | Nachweisverfahren von induzierten Lichtsignalen in einer dreidimensionalen Region einer Probe |
| GB2583061B (en) * | 2019-02-12 | 2023-03-15 | Advanced Risc Mach Ltd | Data processing systems |
| JP7329951B2 (ja) * | 2019-04-01 | 2023-08-21 | キヤノン株式会社 | 画像処理装置およびその制御方法 |
| KR102719078B1 (ko) | 2021-10-26 | 2024-10-24 | 포항공과대학교 산학협력단 | 목시플록사신을 이용한 생체조직표면 세포 영상검사장치 및 목시플록사신을 이용한 생체조직표면 세포 영상검사방법 |
Citations (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006127692A2 (en) | 2005-05-23 | 2006-11-30 | Hess Harald F | Optical microscopy with phototransformable optical labels |
| DE102006021317B3 (de) | 2006-05-06 | 2007-10-11 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Verfahren und Fluoreszenzlichtmikroskop zum räumlich hochauflösenden Abbilden einer Struktur einer Probe |
| US20090059360A1 (en) | 2007-08-31 | 2009-03-05 | The General Hospital Corporation | System and method for self-interference fluorescence microscopy, and computer-accessible medium associated therewith |
| DE102008009216A1 (de) | 2008-02-13 | 2009-08-20 | Carl Zeiss Microimaging Gmbh | Vorrichtung und Verfahren zum räumlich hochauflösenden Abbilden einer Struktur einer Probe |
| US20090237501A1 (en) | 2008-03-19 | 2009-09-24 | Ruprecht-Karis-Universitat Heidelberg Kirchhoff-Institut Fur Physik | method and an apparatus for localization of single dye molecules in the fluorescent microscopy |
| US20090242798A1 (en) | 2008-04-01 | 2009-10-01 | The Jackson Laboratory | 3D Biplane Microscopy |
| US20090242801A1 (en) | 2008-03-31 | 2009-10-01 | Deutsches Krebsforschungszentrum | Method of fluorescence-microscopically imaging a structure in a sample with high three-dimensional spatial resolution |
| US20090263002A1 (en) | 2008-04-17 | 2009-10-22 | Ruprecht-Karls-Universitat Heidelberg | Wave field microscope with sub-wavelength resolution and methods for processing microscopic images to detect objects with sub-wavelength dimensions |
| DE102008024568A1 (de) | 2008-05-21 | 2009-12-03 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Verfahren zum räumlich hochauflösenden Abbilden einer interessierenden Struktur einer Probe |
| US7675045B1 (en) | 2008-10-09 | 2010-03-09 | Los Alamos National Security, Llc | 3-dimensional imaging at nanometer resolutions |
| DE102008049886A1 (de) | 2008-09-30 | 2010-04-01 | Carl Zeiss Microimaging Gmbh | Vorrichtung, insbesondere ein Mikroskop, zur Untersuchung von Proben |
| DE102008059328A1 (de) | 2008-11-27 | 2010-06-02 | Carl Zeiss Microimaging Gmbh | Auflösungsgesteigerte Mikroskopie |
| US20100160613A1 (en) | 2008-12-22 | 2010-06-24 | Leica Microsystems Cms Gmbh | Method for high spatial resolution stochastic examination of a sample structure labeled with a substance |
| US20100207037A1 (en) | 2009-01-26 | 2010-08-19 | The General Hospital Corporation | System, method and computer-accessible medium for providing wide-field superresolution microscopy |
| US20100283835A1 (en) | 2007-01-11 | 2010-11-11 | Joerg Bewersdorf | Microscopic imaging techniques |
| DE102009043744A1 (de) * | 2009-09-30 | 2011-03-31 | Carl Zeiss Microlmaging Gmbh | Verfahren und Mikroskop zur dreidimensional auflösungsgesteigerten Mikroskopie |
| DE102009060490A1 (de) | 2009-12-22 | 2011-06-30 | Carl Zeiss Microlmaging GmbH, 07745 | Hochauflösendes Mikroskop und Bildteileranordnung |
| WO2012039636A2 (en) * | 2010-09-24 | 2012-03-29 | Christian Soeller | "3d localisation microscopy and 4d localisation microscopy and tracking methods and systems" |
| EP2437097A2 (de) | 2010-09-30 | 2012-04-04 | Carl Zeiss MicroImaging GmbH | Mikroskopsystem, Mikroskopieverfahren und Computerprogrammprodukt |
| DE102010044013A1 (de) | 2010-11-16 | 2012-05-16 | Carl Zeiss Microimaging Gmbh | Tiefenauflösungsgesteigerte Mikroskopie |
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| JP2001307366A (ja) * | 1999-08-02 | 2001-11-02 | Matsushita Electric Ind Co Ltd | 光ピックアップ装置 |
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| US6947127B2 (en) * | 2001-12-10 | 2005-09-20 | Carl Zeiss Jena Gmbh | Arrangement for the optical capture of excited and/or back scattered light beam in a sample |
| US6888148B2 (en) * | 2001-12-10 | 2005-05-03 | Carl Zeiss Jena Gmbh | Arrangement for the optical capture of excited and /or back scattered light beam in a sample |
| DE10227119A1 (de) * | 2002-06-15 | 2004-01-15 | Carl Zeiss Jena Gmbh | Optische Anordnung zur Gewinnung von Informationen von einer Probe oder einem Beobachtungsobjekt |
| DE10227120A1 (de) * | 2002-06-15 | 2004-03-04 | Carl Zeiss Jena Gmbh | Mikroskop, insbesondere Laserscanningmikroskop mit adaptiver optischer Einrichtung |
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-
2012
- 2012-01-24 DE DE102012201003.3A patent/DE102012201003B4/de active Active
- 2012-12-13 JP JP2014552544A patent/JP6148256B2/ja active Active
- 2012-12-13 WO PCT/EP2012/075465 patent/WO2013110408A1/de not_active Ceased
- 2012-12-13 US US14/373,740 patent/US9885860B2/en active Active
- 2012-12-13 EP EP12809201.2A patent/EP2807515B1/de active Active
Patent Citations (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006127692A3 (en) | 2005-05-23 | 2007-04-26 | Harald F Hess | Optical microscopy with phototransformable optical labels |
| WO2006127692A2 (en) | 2005-05-23 | 2006-11-30 | Hess Harald F | Optical microscopy with phototransformable optical labels |
| DE102006021317B3 (de) | 2006-05-06 | 2007-10-11 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Verfahren und Fluoreszenzlichtmikroskop zum räumlich hochauflösenden Abbilden einer Struktur einer Probe |
| US20090134342A1 (en) | 2006-05-06 | 2009-05-28 | Stefan Hell | High spatial resolution imaging of a structure of interest in a specimen |
| US20100283835A1 (en) | 2007-01-11 | 2010-11-11 | Joerg Bewersdorf | Microscopic imaging techniques |
| US20090059360A1 (en) | 2007-08-31 | 2009-03-05 | The General Hospital Corporation | System and method for self-interference fluorescence microscopy, and computer-accessible medium associated therewith |
| DE102008009216A1 (de) | 2008-02-13 | 2009-08-20 | Carl Zeiss Microimaging Gmbh | Vorrichtung und Verfahren zum räumlich hochauflösenden Abbilden einer Struktur einer Probe |
| US20090237501A1 (en) | 2008-03-19 | 2009-09-24 | Ruprecht-Karis-Universitat Heidelberg Kirchhoff-Institut Fur Physik | method and an apparatus for localization of single dye molecules in the fluorescent microscopy |
| US20090242801A1 (en) | 2008-03-31 | 2009-10-01 | Deutsches Krebsforschungszentrum | Method of fluorescence-microscopically imaging a structure in a sample with high three-dimensional spatial resolution |
| US20090242798A1 (en) | 2008-04-01 | 2009-10-01 | The Jackson Laboratory | 3D Biplane Microscopy |
| US20090263002A1 (en) | 2008-04-17 | 2009-10-22 | Ruprecht-Karls-Universitat Heidelberg | Wave field microscope with sub-wavelength resolution and methods for processing microscopic images to detect objects with sub-wavelength dimensions |
| DE102008024568A1 (de) | 2008-05-21 | 2009-12-03 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Verfahren zum räumlich hochauflösenden Abbilden einer interessierenden Struktur einer Probe |
| DE102008049886A1 (de) | 2008-09-30 | 2010-04-01 | Carl Zeiss Microimaging Gmbh | Vorrichtung, insbesondere ein Mikroskop, zur Untersuchung von Proben |
| US7675045B1 (en) | 2008-10-09 | 2010-03-09 | Los Alamos National Security, Llc | 3-dimensional imaging at nanometer resolutions |
| DE102008059328A1 (de) | 2008-11-27 | 2010-06-02 | Carl Zeiss Microimaging Gmbh | Auflösungsgesteigerte Mikroskopie |
| US20100160613A1 (en) | 2008-12-22 | 2010-06-24 | Leica Microsystems Cms Gmbh | Method for high spatial resolution stochastic examination of a sample structure labeled with a substance |
| US20100207037A1 (en) | 2009-01-26 | 2010-08-19 | The General Hospital Corporation | System, method and computer-accessible medium for providing wide-field superresolution microscopy |
| DE102009043744A1 (de) * | 2009-09-30 | 2011-03-31 | Carl Zeiss Microlmaging Gmbh | Verfahren und Mikroskop zur dreidimensional auflösungsgesteigerten Mikroskopie |
| DE102009060490A1 (de) | 2009-12-22 | 2011-06-30 | Carl Zeiss Microlmaging GmbH, 07745 | Hochauflösendes Mikroskop und Bildteileranordnung |
| WO2012039636A2 (en) * | 2010-09-24 | 2012-03-29 | Christian Soeller | "3d localisation microscopy and 4d localisation microscopy and tracking methods and systems" |
| EP2437097A2 (de) | 2010-09-30 | 2012-04-04 | Carl Zeiss MicroImaging GmbH | Mikroskopsystem, Mikroskopieverfahren und Computerprogrammprodukt |
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| www.bostonmicromachines.com/light-modulator.htm oder www.imagine-optic.com |
Also Published As
| Publication number | Publication date |
|---|---|
| EP2807515B1 (de) | 2016-03-09 |
| EP2807515A1 (de) | 2014-12-03 |
| JP6148256B2 (ja) | 2017-06-14 |
| JP2015505620A (ja) | 2015-02-23 |
| DE102012201003A1 (de) | 2013-07-25 |
| US20150160446A1 (en) | 2015-06-11 |
| WO2013110408A1 (de) | 2013-08-01 |
| US9885860B2 (en) | 2018-02-06 |
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