DE102009031231A1 - Verfahren und Anordnungen für die Fluoreszenzmikroskopie - Google Patents
Verfahren und Anordnungen für die Fluoreszenzmikroskopie Download PDFInfo
- Publication number
- DE102009031231A1 DE102009031231A1 DE102009031231A DE102009031231A DE102009031231A1 DE 102009031231 A1 DE102009031231 A1 DE 102009031231A1 DE 102009031231 A DE102009031231 A DE 102009031231A DE 102009031231 A DE102009031231 A DE 102009031231A DE 102009031231 A1 DE102009031231 A1 DE 102009031231A1
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- Germany
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- microscope
- region
- fluorescence
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- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 238000000034 method Methods 0.000 title claims abstract description 39
- 238000000799 fluorescence microscopy Methods 0.000 title abstract description 4
- 238000001000 micrograph Methods 0.000 claims abstract description 58
- 238000009826 distribution Methods 0.000 claims abstract description 25
- 238000011156 evaluation Methods 0.000 claims abstract description 10
- 238000012546 transfer Methods 0.000 claims abstract description 9
- 238000001514 detection method Methods 0.000 claims description 16
- 230000005284 excitation Effects 0.000 claims description 12
- 238000005286 illumination Methods 0.000 claims description 9
- 238000005259 measurement Methods 0.000 claims description 8
- 230000002123 temporal effect Effects 0.000 claims description 8
- 238000004364 calculation method Methods 0.000 claims description 6
- 239000007850 fluorescent dye Substances 0.000 claims description 6
- 230000005855 radiation Effects 0.000 claims description 5
- 238000004590 computer program Methods 0.000 claims description 3
- 230000001131 transforming effect Effects 0.000 claims description 3
- 239000003086 colorant Substances 0.000 claims description 2
- 230000001419 dependent effect Effects 0.000 claims description 2
- 230000005540 biological transmission Effects 0.000 claims 1
- 230000005484 gravity Effects 0.000 claims 1
- 238000002165 resonance energy transfer Methods 0.000 claims 1
- 238000011158 quantitative evaluation Methods 0.000 abstract description 18
- 230000006872 improvement Effects 0.000 abstract description 3
- 238000011160 research Methods 0.000 abstract description 2
- 239000000779 smoke Substances 0.000 abstract 1
- 230000004913 activation Effects 0.000 description 8
- 238000000386 microscopy Methods 0.000 description 8
- 230000004807 localization Effects 0.000 description 6
- 238000004061 bleaching Methods 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 238000003384 imaging method Methods 0.000 description 4
- 230000009466 transformation Effects 0.000 description 4
- 238000002060 fluorescence correlation spectroscopy Methods 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 238000000090 raster image correlation spectroscopy Methods 0.000 description 3
- 230000002441 reversible effect Effects 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 2
- 229910052791 calcium Inorganic materials 0.000 description 2
- 239000011575 calcium Substances 0.000 description 2
- 238000004624 confocal microscopy Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- BUHVIAUBTBOHAG-FOYDDCNASA-N (2r,3r,4s,5r)-2-[6-[[2-(3,5-dimethoxyphenyl)-2-(2-methylphenyl)ethyl]amino]purin-9-yl]-5-(hydroxymethyl)oxolane-3,4-diol Chemical compound COC1=CC(OC)=CC(C(CNC=2C=3N=CN(C=3N=CN=2)[C@H]2[C@@H]([C@H](O)[C@@H](CO)O2)O)C=2C(=CC=CC=2)C)=C1 BUHVIAUBTBOHAG-FOYDDCNASA-N 0.000 description 1
- 238000001327 Förster resonance energy transfer Methods 0.000 description 1
- 102000007474 Multiprotein Complexes Human genes 0.000 description 1
- 108010085220 Multiprotein Complexes Proteins 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000002866 fluorescence resonance energy transfer Methods 0.000 description 1
- 230000005283 ground state Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000013307 optical fiber Substances 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000001454 recorded image Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 238000004611 spectroscopical analysis Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 230000004304 visual acuity Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/008—Details of detection or image processing, including general computer control
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
Landscapes
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Optics & Photonics (AREA)
- Health & Medical Sciences (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Computer Vision & Pattern Recognition (AREA)
- General Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Microscoopes, Condenser (AREA)
Priority Applications (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102009031231A DE102009031231A1 (de) | 2009-06-26 | 2009-06-26 | Verfahren und Anordnungen für die Fluoreszenzmikroskopie |
| JP2012516561A JP5746161B2 (ja) | 2009-06-26 | 2010-06-19 | 顕微鏡画像における蛍光の評価方法 |
| EP10728594.2A EP2446314B1 (de) | 2009-06-26 | 2010-06-19 | Verfahren zum auswerten von fluoreszenzereignissen in einem mikroskopbild |
| US13/380,171 US8988771B2 (en) | 2009-06-26 | 2010-06-19 | Method for evaluating fluorescence results in a microscope image |
| PCT/EP2010/003705 WO2010149319A1 (de) | 2009-06-26 | 2010-06-19 | Verfahren zum auswerten von fluoreszenzereignissen in einem mikroskopbild |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102009031231A DE102009031231A1 (de) | 2009-06-26 | 2009-06-26 | Verfahren und Anordnungen für die Fluoreszenzmikroskopie |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE102009031231A1 true DE102009031231A1 (de) | 2010-12-30 |
Family
ID=42357548
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE102009031231A Ceased DE102009031231A1 (de) | 2009-06-26 | 2009-06-26 | Verfahren und Anordnungen für die Fluoreszenzmikroskopie |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US8988771B2 (https=) |
| EP (1) | EP2446314B1 (https=) |
| JP (1) | JP5746161B2 (https=) |
| DE (1) | DE102009031231A1 (https=) |
| WO (1) | WO2010149319A1 (https=) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102012216164A1 (de) * | 2012-09-12 | 2014-03-13 | Forschungsverbund Berlin E.V. | Vorrichtung mit einer Anordnung optischer Elemente |
| EP3051276A4 (en) * | 2013-09-27 | 2017-05-31 | Nikon Corporation | Analysis device, microscope device, analysis method, and program |
| DE102015016353A1 (de) | 2015-12-16 | 2017-06-22 | Horst Wochnowski | PALM-Verfahren mit mehreren sich nur marginal voneinander unterscheidenden Fluorophoren |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201318598D0 (en) * | 2013-10-21 | 2013-12-04 | Univ Leicester | Improvements in or relating to super-resolution microscopy |
| US20210010920A1 (en) * | 2017-12-27 | 2021-01-14 | Riken | Spectroscopic analysis device, spectroscopic analysis method, program, recording medium, and microscope |
| US11169368B2 (en) * | 2018-01-02 | 2021-11-09 | King's College London | Method and system for localisation microscopy |
| US11674902B2 (en) * | 2018-07-16 | 2023-06-13 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. | System and method for use in fret microscopy |
| DE102018122816B9 (de) * | 2018-09-18 | 2024-07-04 | Carl Zeiss Meditec Ag | Verfahren und Vorrichtung zum Bestimmen einer Eigenschaft eines Objekts |
| DE102018220779A1 (de) | 2018-12-03 | 2020-06-04 | Carl Zeiss Microscopy Gmbh | Nachweisverfahren von induzierten Lichtsignalen in einer dreidimensionalen Region einer Probe |
| DE102019127775A1 (de) * | 2019-10-15 | 2021-04-15 | Carl Zeiss Microscopy Gmbh | Lichtmikroskop und Verfahren zur Bildaufnahme mit einem Lichtmikroskop |
| CN115190969A (zh) * | 2020-01-16 | 2022-10-14 | 耶路撒冷希伯来大学伊森姆研究发展有限公司 | 用于fret显微镜学中使用的系统和方法 |
| JP7565554B2 (ja) * | 2020-05-22 | 2024-10-11 | 国立大学法人山梨大学 | 分光計、分析システム及び分析方法 |
| DE102020006975A1 (de) * | 2020-11-11 | 2022-05-12 | Carl Zeiss Microscopy Gmbh | Laser-Scanning-Mikroskop und Verfahren zum Justieren eines Laser-Scanning-Mikroskops |
| DE102020131047B4 (de) * | 2020-11-24 | 2024-08-22 | Abberior Instruments Gmbh | Verfahren und Vorrichtung zur Aufnahme nanoskopischer Bilder mehrfach gefärbter Proben |
| AU2022234898A1 (en) | 2021-03-12 | 2023-09-28 | Stryker European Operations Limited | Neurosurgical methods and systems for detecting and removing tumorous tissue |
Citations (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5671085A (en) | 1995-02-03 | 1997-09-23 | The Regents Of The University Of California | Method and apparatus for three-dimensional microscopy with enhanced depth resolution |
| US6909105B1 (en) | 1999-03-02 | 2005-06-21 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Method and device for representing an object |
| DE102004034998A1 (de) * | 2004-07-16 | 2006-02-02 | Carl Zeiss Jena Gmbh | Lichtrastermikroskop mit bewegter Lochscheibe und Verwendung |
| WO2006127692A2 (en) | 2005-05-23 | 2006-11-30 | Hess Harald F | Optical microscopy with phototransformable optical labels |
| DE102005034443A1 (de) * | 2005-07-22 | 2007-02-22 | Carl Zeiss Jena Gmbh | Auflösungsgesteigerte Lumineszenz-Mikroskopie |
| WO2008032096A2 (en) * | 2006-09-14 | 2008-03-20 | Oxford Gene Technology Ip Limited | Apparatus for imaging single molecules |
| EP1921440A2 (en) * | 1999-11-12 | 2008-05-14 | E.I. Dupont De Nemours And Company | Fluorometer with low heat-generating light source |
| US20080158551A1 (en) * | 2006-12-21 | 2008-07-03 | Hess Harald F | Systems and methods for 3-dimensional interferometric microscopy |
| US20080182336A1 (en) * | 2006-08-07 | 2008-07-31 | President And Fellows Of Harvard College | Sub-diffraction limit image resolution and other imaging techniques |
| EP1985995A2 (en) * | 1996-08-16 | 2008-10-29 | GE Healthcare Niagara Inc. | A digital imaging system for assays in well plates, gels and blots |
| US20090059360A1 (en) * | 2007-08-31 | 2009-03-05 | The General Hospital Corporation | System and method for self-interference fluorescence microscopy, and computer-accessible medium associated therewith |
| DE102008009216A1 (de) * | 2008-02-13 | 2009-08-20 | Carl Zeiss Microimaging Gmbh | Vorrichtung und Verfahren zum räumlich hochauflösenden Abbilden einer Struktur einer Probe |
| US20090237501A1 (en) * | 2008-03-19 | 2009-09-24 | Ruprecht-Karis-Universitat Heidelberg Kirchhoff-Institut Fur Physik | method and an apparatus for localization of single dye molecules in the fluorescent microscopy |
| US20090242801A1 (en) * | 2008-03-31 | 2009-10-01 | Deutsches Krebsforschungszentrum | Method of fluorescence-microscopically imaging a structure in a sample with high three-dimensional spatial resolution |
| US20090242798A1 (en) * | 2008-04-01 | 2009-10-01 | The Jackson Laboratory | 3D Biplane Microscopy |
| DE102008021641A1 (de) * | 2008-04-30 | 2009-11-05 | Carl Zeiss Microlmaging Gmbh | Auflösungsgesteigerte Lumineszenzmikroskopie |
| DE102008028490A1 (de) * | 2008-06-16 | 2009-12-17 | Carl Zeiss Microimaging Gmbh | Beleuchtungsanordnung für die TIRF-Mikroskopie |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5784162A (en) | 1993-08-18 | 1998-07-21 | Applied Spectral Imaging Ltd. | Spectral bio-imaging methods for biological research, medical diagnostics and therapy |
| JP3616107B2 (ja) | 1992-04-10 | 2005-02-02 | 株式会社日立製作所 | ポリヌクレオチド検出法 |
| JP2001509612A (ja) * | 1997-07-10 | 2001-07-24 | ルプレヒト−カールス−ウニヴェルジテート ハイデルベルク | ウェーブフィールド顕微鏡、ウェーブフィールド顕微鏡法、ｄｎａ順序決定のためのウェーブフィールド顕微鏡法、およびウェーブフィールド顕微鏡に対する較正方法 |
| JP2005043278A (ja) | 2003-07-24 | 2005-02-17 | Institute Of Physical & Chemical Research | 蛍光相関分光測定装置及び不均一系試料における拡散係数測定方法 |
| DE10335471A1 (de) | 2003-08-02 | 2005-03-03 | Leica Microsystems Heidelberg Gmbh | Detektor und Verfahren zur Detektion schwacher fluoreszenter Strahlung mit einem Mikroskopsystem |
| JP4323369B2 (ja) | 2004-04-09 | 2009-09-02 | オリンパス株式会社 | 走査型蛍光顕微鏡装置 |
| JP4673000B2 (ja) * | 2004-05-21 | 2011-04-20 | 株式会社キーエンス | 蛍光顕微鏡、蛍光顕微鏡装置を使用した表示方法、蛍光顕微鏡画像表示プログラム及びコンピュータで読み取り可能な記録媒体並びに記憶した機器 |
| WO2006057768A2 (en) * | 2004-11-24 | 2006-06-01 | Battelle Memorial Institute | Optical system for cell imaging |
| EP1848983B1 (en) * | 2005-02-15 | 2013-03-27 | Tata Institute of Fundamental Research | Fluorescence correlation microscopy with real-time alignment readout |
| DE102006021317B3 (de) | 2006-05-06 | 2007-10-11 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Verfahren und Fluoreszenzlichtmikroskop zum räumlich hochauflösenden Abbilden einer Struktur einer Probe |
| AU2007281783A1 (en) * | 2006-08-04 | 2008-02-14 | Ikonisys, Inc. | Image processing method for a microscope system |
| US8174692B2 (en) | 2008-05-21 | 2012-05-08 | Max-Planck-Gesellschaft Zur Foerderung Der Wissenschaften E.V. | High spatial resolution imaging of a structure of interest in a specimen |
| DE102008049886B4 (de) | 2008-09-30 | 2021-11-04 | Carl Zeiss Microscopy Gmbh | Vorrichtung, insbesondere ein Mikroskop, zur Untersuchung von Proben |
| DE102008054317A1 (de) | 2008-11-03 | 2010-05-06 | Carl Zeiss Microlmaging Gmbh | Kombinationsmikroskopie |
| DE102008059328A1 (de) | 2008-11-27 | 2010-06-02 | Carl Zeiss Microimaging Gmbh | Auflösungsgesteigerte Mikroskopie |
| US8693742B2 (en) * | 2008-12-17 | 2014-04-08 | The Regents Of The University Of Colorado | Three-dimensional single-molecule fluorescence imaging beyond the diffraction limit using a double-helix point spread function |
-
2009
- 2009-06-26 DE DE102009031231A patent/DE102009031231A1/de not_active Ceased
-
2010
- 2010-06-19 WO PCT/EP2010/003705 patent/WO2010149319A1/de not_active Ceased
- 2010-06-19 JP JP2012516561A patent/JP5746161B2/ja active Active
- 2010-06-19 US US13/380,171 patent/US8988771B2/en active Active
- 2010-06-19 EP EP10728594.2A patent/EP2446314B1/de active Active
Patent Citations (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5671085A (en) | 1995-02-03 | 1997-09-23 | The Regents Of The University Of California | Method and apparatus for three-dimensional microscopy with enhanced depth resolution |
| EP1985995A2 (en) * | 1996-08-16 | 2008-10-29 | GE Healthcare Niagara Inc. | A digital imaging system for assays in well plates, gels and blots |
| US6909105B1 (en) | 1999-03-02 | 2005-06-21 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Method and device for representing an object |
| EP1921440A2 (en) * | 1999-11-12 | 2008-05-14 | E.I. Dupont De Nemours And Company | Fluorometer with low heat-generating light source |
| DE102004034998A1 (de) * | 2004-07-16 | 2006-02-02 | Carl Zeiss Jena Gmbh | Lichtrastermikroskop mit bewegter Lochscheibe und Verwendung |
| WO2006127692A2 (en) | 2005-05-23 | 2006-11-30 | Hess Harald F | Optical microscopy with phototransformable optical labels |
| DE102005034443A1 (de) * | 2005-07-22 | 2007-02-22 | Carl Zeiss Jena Gmbh | Auflösungsgesteigerte Lumineszenz-Mikroskopie |
| US20080182336A1 (en) * | 2006-08-07 | 2008-07-31 | President And Fellows Of Harvard College | Sub-diffraction limit image resolution and other imaging techniques |
| WO2008032096A2 (en) * | 2006-09-14 | 2008-03-20 | Oxford Gene Technology Ip Limited | Apparatus for imaging single molecules |
| US20080158551A1 (en) * | 2006-12-21 | 2008-07-03 | Hess Harald F | Systems and methods for 3-dimensional interferometric microscopy |
| US20090059360A1 (en) * | 2007-08-31 | 2009-03-05 | The General Hospital Corporation | System and method for self-interference fluorescence microscopy, and computer-accessible medium associated therewith |
| DE102008009216A1 (de) * | 2008-02-13 | 2009-08-20 | Carl Zeiss Microimaging Gmbh | Vorrichtung und Verfahren zum räumlich hochauflösenden Abbilden einer Struktur einer Probe |
| US20090237501A1 (en) * | 2008-03-19 | 2009-09-24 | Ruprecht-Karis-Universitat Heidelberg Kirchhoff-Institut Fur Physik | method and an apparatus for localization of single dye molecules in the fluorescent microscopy |
| US20090242801A1 (en) * | 2008-03-31 | 2009-10-01 | Deutsches Krebsforschungszentrum | Method of fluorescence-microscopically imaging a structure in a sample with high three-dimensional spatial resolution |
| US20090242798A1 (en) * | 2008-04-01 | 2009-10-01 | The Jackson Laboratory | 3D Biplane Microscopy |
| DE102008021641A1 (de) * | 2008-04-30 | 2009-11-05 | Carl Zeiss Microlmaging Gmbh | Auflösungsgesteigerte Lumineszenzmikroskopie |
| DE102008028490A1 (de) * | 2008-06-16 | 2009-12-17 | Carl Zeiss Microimaging Gmbh | Beleuchtungsanordnung für die TIRF-Mikroskopie |
Non-Patent Citations (3)
| Title |
|---|
| Heilemann et al., Angew. Chem. Int. Ed. 2008, 47, 6172, und dort zitierte Referenzen |
| Pawley, S. 529 |
| Pawley: "Biological Confocal Microscopy", Springer Verlag, 3. Auflage 2006, S. 6 |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102012216164A1 (de) * | 2012-09-12 | 2014-03-13 | Forschungsverbund Berlin E.V. | Vorrichtung mit einer Anordnung optischer Elemente |
| DE102012216164B4 (de) * | 2012-09-12 | 2016-04-28 | Forschungsverbund Berlin E.V. | Vorrichtung mit einer Anordnung optischer Elemente |
| EP3051276A4 (en) * | 2013-09-27 | 2017-05-31 | Nikon Corporation | Analysis device, microscope device, analysis method, and program |
| US10268033B2 (en) | 2013-09-27 | 2019-04-23 | Nikon Corporation | Analysis device, microscope device, analysis method, and program |
| US10527838B2 (en) | 2013-09-27 | 2020-01-07 | Nikon Corporation | Analysis device, microscope device, analysis method, and program |
| EP3051276B1 (en) | 2013-09-27 | 2021-03-31 | Nikon Corporation | Analysis device, microscope device, analysis method, and program |
| DE102015016353A1 (de) | 2015-12-16 | 2017-06-22 | Horst Wochnowski | PALM-Verfahren mit mehreren sich nur marginal voneinander unterscheidenden Fluorophoren |
Also Published As
| Publication number | Publication date |
|---|---|
| EP2446314A1 (de) | 2012-05-02 |
| US8988771B2 (en) | 2015-03-24 |
| US20120140317A1 (en) | 2012-06-07 |
| WO2010149319A1 (de) | 2010-12-29 |
| EP2446314B1 (de) | 2019-10-23 |
| JP5746161B2 (ja) | 2015-07-08 |
| JP2012530947A (ja) | 2012-12-06 |
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