DE102009002577A8 - Zellkulturträger für die Kultivierung von humanen Stamm- und Vorläuferzellen sowie ein Verfahren zur Kultivierung - Google Patents

Zellkulturträger für die Kultivierung von humanen Stamm- und Vorläuferzellen sowie ein Verfahren zur Kultivierung Download PDF

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Publication number
DE102009002577A8
DE102009002577A8 DE102009002577A DE102009002577A DE102009002577A8 DE 102009002577 A8 DE102009002577 A8 DE 102009002577A8 DE 102009002577 A DE102009002577 A DE 102009002577A DE 102009002577 A DE102009002577 A DE 102009002577A DE 102009002577 A8 DE102009002577 A8 DE 102009002577A8
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Prior art keywords
cultivation
cell culture
progenitor cells
human stem
culture carrier
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DE102009002577A
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DE102009002577A1 (de
DE102009002577B4 (de
Inventor
Tilo Dr. rer. nat. Pompe
Ina Dipl.-Biol. Kurth
Katja Dipl.-Ing. Franke
Carsten Prof. Dr.rer.nat. Werner
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Leibniz Institut fuer Polymerforschung Dresden eV
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Leibniz Institut fuer Polymerforschung Dresden eV
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Priority to DE102009002577.4A priority Critical patent/DE102009002577B4/de
Priority to PCT/EP2010/055246 priority patent/WO2010122044A2/de
Publication of DE102009002577A1 publication Critical patent/DE102009002577A1/de
Publication of DE102009002577A8 publication Critical patent/DE102009002577A8/de
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Publication of DE102009002577B4 publication Critical patent/DE102009002577B4/de
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/145—Thrombopoietin [TPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/26—Flt-3 ligand (CD135L, flk-2 ligand)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2535/00—Supports or coatings for cell culture characterised by topography
    • C12N2535/10—Patterned coating

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DE102009002577.4A 2009-04-22 2009-04-22 Zellkulturträger für die Kultivierung von humanen Stamm- und Vorläuferzellen sowie ein Verfahren zur Kultivierung Expired - Fee Related DE102009002577B4 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE102009002577.4A DE102009002577B4 (de) 2009-04-22 2009-04-22 Zellkulturträger für die Kultivierung von humanen Stamm- und Vorläuferzellen sowie ein Verfahren zur Kultivierung
PCT/EP2010/055246 WO2010122044A2 (de) 2009-04-22 2010-04-21 Zellkulturträger für die kultivierung von humanen stamm- und vorläuferzellen sowie ein verfahren zur kultivierung

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102009002577.4A DE102009002577B4 (de) 2009-04-22 2009-04-22 Zellkulturträger für die Kultivierung von humanen Stamm- und Vorläuferzellen sowie ein Verfahren zur Kultivierung

Publications (3)

Publication Number Publication Date
DE102009002577A1 DE102009002577A1 (de) 2010-10-28
DE102009002577A8 true DE102009002577A8 (de) 2011-04-07
DE102009002577B4 DE102009002577B4 (de) 2018-01-18

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DE102009002577.4A Expired - Fee Related DE102009002577B4 (de) 2009-04-22 2009-04-22 Zellkulturträger für die Kultivierung von humanen Stamm- und Vorläuferzellen sowie ein Verfahren zur Kultivierung

Country Status (2)

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DE (1) DE102009002577B4 (de)
WO (1) WO2010122044A2 (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG184204A1 (en) 2010-03-23 2012-10-30 Kuraray Co Culture method for causing differentiation of pluripotent mammalian cells
US20120052579A1 (en) * 2010-08-27 2012-03-01 Simon Kelly Shannon Peptide-modified microcarriers for cell culture
DE102011002839B4 (de) * 2011-01-18 2015-02-05 Leibniz-Institut Für Polymerforschung Dresden E.V. Verfahren zur Immobilisierung und Aufbereitung funktioneller Multikomponentenstrukturen der extrazellulären Matrix
CN103146572B (zh) * 2011-12-07 2016-01-06 清华大学 一种实现细胞集落均质性生长的装置和方法
WO2020177789A1 (de) * 2019-03-06 2020-09-10 Zellwerk Gmbh Zellkulturträger für bioreaktoren
DE102019001604B4 (de) * 2019-03-06 2020-12-24 Zellwerk Gmbh Zellkulturträger für Bioreaktoren

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10320421A1 (de) 2002-11-29 2004-06-09 Müller-Schulte, Detlef, Dr. Mikrostrukturierte Polymerträger mit speziellen Formaten für die medizinische, biochemische und molekularbiologische Analytik und Diagnostik
DE10315930A1 (de) 2003-03-31 2004-10-21 Institut Für Polymerforschung Dresden E.V. Verfahren zur Vaskularisierung von künstlichen Zellkulturträgern
US20050208644A1 (en) 2004-03-22 2005-09-22 Seiko Epson Corporation Method of immobilizing cells on solid-phase surface
DE102007055865B3 (de) 2007-12-19 2009-10-01 Leibniz-Institut Für Polymerforschung Dresden E.V. Modifizierte Multi-Well-Platte für biochemische Analysen und Zellkulturexperimente

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000300248A (ja) * 1999-04-14 2000-10-31 Japan Steel Works Ltd:The 細胞培養用基材
JP2005333938A (ja) * 2004-05-31 2005-12-08 Fujitsu Ltd 細胞固定用部材、細胞固定方法、および細胞固定用部材作製装置

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10320421A1 (de) 2002-11-29 2004-06-09 Müller-Schulte, Detlef, Dr. Mikrostrukturierte Polymerträger mit speziellen Formaten für die medizinische, biochemische und molekularbiologische Analytik und Diagnostik
DE10315930A1 (de) 2003-03-31 2004-10-21 Institut Für Polymerforschung Dresden E.V. Verfahren zur Vaskularisierung von künstlichen Zellkulturträgern
US20050208644A1 (en) 2004-03-22 2005-09-22 Seiko Epson Corporation Method of immobilizing cells on solid-phase surface
DE102007055865B3 (de) 2007-12-19 2009-10-01 Leibniz-Institut Für Polymerforschung Dresden E.V. Modifizierte Multi-Well-Platte für biochemische Analysen und Zellkulturexperimente

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
Herklotz, M. et al., Microfluid Nanofluid 3, 2007, S. 629 Abstract
JP 2000-300248 A (Abstract)
JP 2005-333938 A (Abstract)
Oswald, J. et al., Stem Cells 24, 2006 494-500
PubMed PMID 15244449 (Abstract zu: Salchert, K. et al., Biomacromolecules 5(4), 2004, S. 1340-50)
PubMed PMID 17034846 (Abstract zu: Franke, K. et al., Biomaterials 5, 2007, S.836-43)

Also Published As

Publication number Publication date
WO2010122044A3 (de) 2011-01-27
DE102009002577A1 (de) 2010-10-28
WO2010122044A2 (de) 2010-10-28
DE102009002577B4 (de) 2018-01-18

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