DE10158516A1 - Verfahren zum Nachweis von Hybridisierungsereignissen in Nukleinsäuren - Google Patents
Verfahren zum Nachweis von Hybridisierungsereignissen in NukleinsäurenInfo
- Publication number
- DE10158516A1 DE10158516A1 DE10158516A DE10158516A DE10158516A1 DE 10158516 A1 DE10158516 A1 DE 10158516A1 DE 10158516 A DE10158516 A DE 10158516A DE 10158516 A DE10158516 A DE 10158516A DE 10158516 A1 DE10158516 A1 DE 10158516A1
- Authority
- DE
- Germany
- Prior art keywords
- nucleic acid
- nucleic acids
- probe
- probe nucleic
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (10)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10158516A DE10158516A1 (de) | 2001-11-29 | 2001-11-29 | Verfahren zum Nachweis von Hybridisierungsereignissen in Nukleinsäuren |
| AU2002352121A AU2002352121A1 (en) | 2001-11-29 | 2002-11-25 | Method for detecting hybridization events in nucleic acids |
| EP02787794A EP1453975B1 (de) | 2001-11-29 | 2002-11-25 | Verfahren zum nachweis von hybridisierungsereignissen in nukleinsäuren |
| PCT/EP2002/013215 WO2003046215A1 (de) | 2001-11-29 | 2002-11-25 | Verfahren zum nachweis von hybridisierungsereignissen in nukleinsäuren |
| AT02787794T ATE335853T1 (de) | 2001-11-29 | 2002-11-25 | Verfahren zum nachweis von hybridisierungsereignissen in nukleinsäuren |
| CNA028265599A CN1610757A (zh) | 2001-11-29 | 2002-11-25 | 检测核酸中杂化作用的方法 |
| CA002468411A CA2468411A1 (en) | 2001-11-29 | 2002-11-25 | Method for detecting hybridization events in nucleic acids |
| DE50207820T DE50207820D1 (de) | 2001-11-29 | 2002-11-25 | Verfahren zum nachweis von hybridisierungsereignissen in nukleinsäuren |
| JP2003547646A JP2005536983A (ja) | 2001-11-29 | 2002-11-25 | 核酸におけるハイブリダイゼーション事象を検出するための方法 |
| US10/497,130 US20060057569A1 (en) | 2001-11-29 | 2002-11-25 | Method for detecting hybridization events in nucleic acids |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10158516A DE10158516A1 (de) | 2001-11-29 | 2001-11-29 | Verfahren zum Nachweis von Hybridisierungsereignissen in Nukleinsäuren |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE10158516A1 true DE10158516A1 (de) | 2003-06-12 |
Family
ID=7707346
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE10158516A Withdrawn DE10158516A1 (de) | 2001-11-29 | 2001-11-29 | Verfahren zum Nachweis von Hybridisierungsereignissen in Nukleinsäuren |
| DE50207820T Expired - Fee Related DE50207820D1 (de) | 2001-11-29 | 2002-11-25 | Verfahren zum nachweis von hybridisierungsereignissen in nukleinsäuren |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE50207820T Expired - Fee Related DE50207820D1 (de) | 2001-11-29 | 2002-11-25 | Verfahren zum nachweis von hybridisierungsereignissen in nukleinsäuren |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20060057569A1 (https=) |
| EP (1) | EP1453975B1 (https=) |
| JP (1) | JP2005536983A (https=) |
| CN (1) | CN1610757A (https=) |
| AT (1) | ATE335853T1 (https=) |
| AU (1) | AU2002352121A1 (https=) |
| CA (1) | CA2468411A1 (https=) |
| DE (2) | DE10158516A1 (https=) |
| WO (1) | WO2003046215A1 (https=) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9063156B2 (en) * | 2009-06-12 | 2015-06-23 | Pacific Biosciences Of California, Inc. | Real-time analytical methods and systems |
| CN102859003B (zh) | 2010-03-08 | 2014-07-30 | 达纳-法伯癌症研究院有限公司 | 使用参考封闭序列的全cold-pcr富集 |
| EP3333269B1 (en) | 2011-03-31 | 2021-05-05 | Dana-Farber Cancer Institute, Inc. | Methods to enable multiplex cold-pcr |
| CA2990846C (en) * | 2015-06-24 | 2023-09-26 | Dana-Farber Cancer Institute, Inc. | Selective degradation of wild-type dna and enrichment of mutant alleles using nuclease |
| CN109790577B (zh) | 2016-08-01 | 2024-02-09 | 豪夫迈·罗氏有限公司 | 从核酸测序制备物除去衔接子二聚体的方法 |
| US11371090B2 (en) | 2016-12-12 | 2022-06-28 | Dana-Farber Cancer Institute, Inc. | Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection |
| JP2019154396A (ja) * | 2018-03-16 | 2019-09-19 | 株式会社リコー | 核酸検出方法、並びに、それに用いる標的核酸検出用プローブ、及び標的核酸検出用デバイス |
| JP7035972B2 (ja) * | 2018-11-09 | 2022-03-15 | 横河電機株式会社 | 核酸配列計測用デバイス |
| US20220372560A1 (en) * | 2019-10-30 | 2022-11-24 | Takeda Pharmaceutical Company Limited | Methods for detecting oligonucleotides |
| CN114280128B (zh) * | 2021-12-24 | 2022-11-18 | 清华大学 | 双标记gFET的制备及其在miRNA检测中的应用 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5543292A (en) * | 1992-06-16 | 1996-08-06 | Hitachi, Ltd. | Process for the measurement of nucleic acids |
| US5858659A (en) * | 1995-11-29 | 1999-01-12 | Affymetrix, Inc. | Polymorphism detection |
| EP0730662A4 (en) * | 1993-09-10 | 1999-11-24 | Genevue Inc | OPTICAL DETECTION OF THE POSITION OF OLIGONUCLEOTIDES ON LARGE DNA MOLECULES |
| US5925517A (en) * | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| WO2002024955A2 (en) * | 2000-09-19 | 2002-03-28 | Atom Sciences, Inc. | Detection of unlabeled hybridized dna and rna using restriction enzyme digestion |
-
2001
- 2001-11-29 DE DE10158516A patent/DE10158516A1/de not_active Withdrawn
-
2002
- 2002-11-25 US US10/497,130 patent/US20060057569A1/en not_active Abandoned
- 2002-11-25 AU AU2002352121A patent/AU2002352121A1/en not_active Abandoned
- 2002-11-25 DE DE50207820T patent/DE50207820D1/de not_active Expired - Fee Related
- 2002-11-25 CA CA002468411A patent/CA2468411A1/en not_active Abandoned
- 2002-11-25 JP JP2003547646A patent/JP2005536983A/ja not_active Ceased
- 2002-11-25 WO PCT/EP2002/013215 patent/WO2003046215A1/de not_active Ceased
- 2002-11-25 CN CNA028265599A patent/CN1610757A/zh active Pending
- 2002-11-25 EP EP02787794A patent/EP1453975B1/de not_active Expired - Lifetime
- 2002-11-25 AT AT02787794T patent/ATE335853T1/de not_active IP Right Cessation
Non-Patent Citations (3)
| Title |
|---|
| BIGGINS,John B.,et.al.: A continuous assay for DNA cleavage: The application of "break lights" to enediynes, iron-dependent agents, and nucleases. In: Proc. Natl. Acad. Sci., USA, 2000, 97, (25), S.13537-13542 * |
| LI,Jianwei Jeffery,et.al.: Using molecular beacons as a sensitive fluorescence assay for enzymatic cleavage of single-stranded DNA. In: Nucleic Acids Research,2000,Vol.28,No.11,S.E52-e52 * |
| STOJANOVIC,M.N.,et.al.: Datenbank PubMed,Zusammenfassung zu: Catalytic molecular beacons. In: Chembiochem., Juni 2001, 1,2 (6), S.411-415 * |
Also Published As
| Publication number | Publication date |
|---|---|
| ATE335853T1 (de) | 2006-09-15 |
| WO2003046215A1 (de) | 2003-06-05 |
| AU2002352121A1 (en) | 2003-06-10 |
| EP1453975B1 (de) | 2006-08-09 |
| JP2005536983A (ja) | 2005-12-08 |
| EP1453975A1 (de) | 2004-09-08 |
| CA2468411A1 (en) | 2003-06-05 |
| CN1610757A (zh) | 2005-04-27 |
| DE50207820D1 (de) | 2006-09-21 |
| US20060057569A1 (en) | 2006-03-16 |
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